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Citations for Thermo Fisher :
8801 - 8850 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The sandwich method was then used to transfer the proteins from the gel to a methanol-activated polyvinylidene fluoride (PVDF) membrane (ThermoFisher Scientific, UK) at a constant current of 30 V for 1 hour and 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg total protein per sample was separated by SDS-PAGE using a NuPAGE™ 4-12 % Tris-Acetate gel (Invitrogen, UK) and NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride membrane [36] and detected with the anti-His (C-terminal)-HRP antibody (Thermo Fisher/Invitrogen) according to the manufacturer's instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... unit of cells in a 1X Laemmli/8 M Urea/ 2.5 % β-mercaptoethanol sample buffer and incubated for 10 minutes at 70 °C prior to SDS-PAGE using 4-12 % Bis-Tris gels (Invitrogen NuPAGE®, Thermo Scientific) in a MOPS buffer (Invitrogen NuPAGE® ...
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bioRxiv - Neuroscience 2020Quote: ... Denatured samples from both the total lysates and surface fractions were separated on NuPAGE 3-8 % Tris-Acetate protein gels (Thermo Fisher Scientific) at 200 V for 40 min ...
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bioRxiv - Molecular Biology 2020Quote: ... protein samples were boiled at 95°C for 5 min before separating on Novex WedgeWell 8–16% Tris-Glycine Mini Gels (Thermo Fisher Scientific). Protein samples were transferred to polyvinylidene fluoride membrane (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... 2-4 × 105 cell equivalents per well were loaded into a NuPAGE 4-12% Bis-Tris density gradient gel (Thermo Fisher Scientific) and ran at a constant 150V for 80 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... DNA quality was determined via agarose gel electrophoresis and the quantity was measured on a NanoDrop (Thermo Fisher Scientific, Waltham, MA, USA). An Illumina paired-end DNA library with an insert size of ∼300 bp was constructed using the Genomic DNA Sample Prep kit (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... VGAT and VGLUT2 DIG antisense riboprobes were transcribed in vitro from purified PCR-amplified DNA fragments (PureLink Quick Gel Extraction Kit, Invitrogen, CA, USA). Forward and reverse primers used were 5’-GCCATTCAGGGCATGTTC-3’ and 5’-AGCAGCGTGAAGACCACC-3’ for VGAT DIG antisense ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions were analyzed by SDS-PAGE with Coomassie staining (10 μL of each sample into a 15-well Bis-Tris NuPAGE gel (Thermo Fisher NP0321BOX), 180V ...
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bioRxiv - Biochemistry 2020Quote: ... Purified RNA amounts were quantified using nanodrop and verified for purity and size using a denaturing agarose gel and Millenium RNA ladder (ThermoFisher Scientific AM7151).
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bioRxiv - Bioengineering 2021Quote: ... Qiagen miniprep Kit`for recombinant plasmid isolation and purification and SDS-NuPAGE precast gels for molecular characterization of expressed enzyme were obtained from Invitrogen (Carlsbad, CA). Polygalacturonic acid (PGA ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were then loaded on a NuPAGE® Novex® 4-12% Bis-Tris Gel and ran at 120 V in MOPES running buffer (Invitrogen). The proteins on the gel were transferred to an iBlot nitrocellulose membrane (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was verified by agarose gel electrophoresis and used for TOPO cloning with the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen). After selection on kanamycin ...
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bioRxiv - Microbiology 2022Quote: ... The integrity of the extracted RNA was evaluated in a 1% (m/v) agarose gel and the material was quantified using the nanodrop (ThermoFisher, Massachusetts, USA).
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bioRxiv - Systems Biology 2022Quote: ... and denatured at 70°C for 10 min prior to loading onto Novex NuPAGE 10% Bis-Tris Protein gels (Invitrogen, Cat.# NP0315BOX) and separated half way (15min at 200 constant voltage) ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA gene PCR products were visualized on an agarose gel stained with SYBR™ safe (Thermo Fisher Scientific, Waltham, MA, USA), purified using GENEJET PCR purification kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... #NP0007)—and boiled at 95°C for 10 minutes before the samples were loaded in the NuPAGE 4%–12% Bis-Tris Gels (Invitrogen, #NP0335BOX & #NP0336BOX) for further resolution and western blot analysis ...
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bioRxiv - Microbiology 2022Quote: ... The samples were sonicated and heated at 72°C for 10 minutes before loading 20 μl/well on NuPAGE™ 3 to 8% Tris-Acetate gels (ThermoFisher, EA03785BOX) alongside high molecular weight markers (ThermoFisher LC5699) ...
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bioRxiv - Neuroscience 2019Quote: ... A total of 5 or 10 μg protein lysates were resolved in Novex 4-20% Tris-Glycine polyacrylamide electrophoresis gels (XP04205BOX; Invitrogen, Carlsbad, CA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Synthetic Biology 2019Quote: ... 20 μL of the supernatant containing the extracted polypeptides was loaded on a NuPAGE Bis Tris 4-12% gels (Thermo Fisher Scientific) for gel electrophoresis and Western blot analysis ...
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bioRxiv - Neuroscience 2019Quote: ... Fractions of pure proteins from the gels were pooled together and the protein stock concentrations were measured using the BCA assay (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2019Quote: ... 125ng of total recombinant protein was separated on 4-20% or 10-20% Novex WedgeWell Tris-Glycine precast gels (Thermofisher, Waltham, MA). For silver stain ...
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bioRxiv - Molecular Biology 2019Quote: ... Isolated NSUN2 was quantified based on the intensity of the corresponding band in an SDS-PAGE gel stained with SYPRO Ruby (Thermo Fisher Scientific), using BSA as a standard.
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bioRxiv - Microbiology 2019Quote: ... 6 µl from each sample was pooled together and the mixture size-selected to ~500-900 bp using a 1.9% agarose gel visualized with SYBR-Safe dye (Thermo Fisher cat. # S33102). DNA was purified from gel slices using a Zymo Research gel DNA recovery kit ...
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bioRxiv - Microbiology 2019Quote: ... Reactions were incubated at 50 °C for 1 h and terminated by the addition of Gel Loading Buffer II (Thermo Fisher Scientific) and incubation at 95 °C for 10 min ...
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bioRxiv - Biophysics 2019Quote: ... heated at 100°C for 5 min and run on a 4 to 12% Bis-Tris SDS-polyacrylamide gel (Life Technologies, CA). Loading amounts of proteins were 1 μg for Coomassie staining and 0.5 μg – for Western Blot ...
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bioRxiv - Bioengineering 2020Quote: ... The quality and quantity of extracted DNA were verified via gel electrophoresis (0.8% agarose) and photometrically using a NanoDrop ND 1000 spectral photometer (Thermo Fisher Scientific, USA). Extracted DNA was stored at −20°C until use ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Whole lung homogenates and bronchoalveolar lavage aliquots were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris gradient gel; Life Technologies, CA) and transferred to PVDF membrane ...
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An engineered receptor-binding domain improves the immunogenicity of multivalent SARS-CoV-2 vaccinesbioRxiv - Microbiology 2020Quote: ... The proteins were mixed with sample buffer and G250 loading dye and added to a 3-12% Bis-Tris NativePAGE™ gel (Life Technologies). BN-PAGE gels were run for 2 hours at 150 V using the NativePAGE™ running buffer (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... Samples were subjected to SDS-Page separation by running 20μg of total protein on a NuPage 4-12% Bis-Tris gel (Thermo Fisher Scientific NP0336BOX) using NuPAGE™ MOPS SDS Running Buffer (Thermo Fisher Scientific NP000102) ...
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bioRxiv - Systems Biology 2021Quote: ... Lysates were separated on Novex NuPAGE Bis-Tris 4-12% polyacrylamide gels with MOPS SDS running buffer (Thermo Fisher #NP0366 and NP0001) and transferred to 0.45 μm PVDF membrane (Millipore #IPFL00010) ...
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bioRxiv - Genomics 2019Quote: ... The agarose plugs were washed with Tris-EDTA buffer the following day and digested at 43°C with GELase™ Agarose Gel-Digesting Prep (2 unit/µl, Thermo Fisher) for 50 min ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein (30-40 µg) were loaded and were separated by electrophoresis in 4 to 12% Bis-Tris Gel (Thermo Fisher Scientific), transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Pathology 2021Quote: ... gels were incubated in 10% ethanol for dehydration before blotting the proteins to a membrane using the BlotTM 2 Gel Transfer Device (Thermo Fisher Inc.). After staining the membranes in Ponceau solution ...
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bioRxiv - Neuroscience 2020Quote: Samples (20 µL containing 10 µg protein) were loaded per well of a NuPage 4-12 % Bis-Tris gradient gels (#NP0336BOX, Thermo Fisher Scientific), and the SDS-Page gel was subsequently transferred to nitrocellulose membranes (#1620115 ...
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bioRxiv - Plant Biology 2020Quote: For staining of nucleoids with SYBR Green I (SYBR™ Green I Nucleic Acid Gel Stain, 10,000X concentrate in DMSO, Invitrogen, Karlsruhe, Germany), cross-sections from the first true leaves of 14 days old Arabidopsis seedlings were excised and fixed overnight at 4°C in 4% (w/v ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and visually evaluated by gel electrophoresis on a 1.2% agarose gel (GelRed nucleic acid stain, Lambda DNA/HindIII Marker and 1kb DNA marker (Invitrogen; Thermo Fisher Scientific, Germany)).
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bioRxiv - Molecular Biology 2021Quote: ... Primer dimers were removed by extracting PCR products using two lanes (10 μL each) of an E-Gel Power Snap Electrophoresis Device (ThermoFisher Scientific, Germany). This resulting library was sequenced on a MiSeq v2 250 bp PE Illumina at CeGaT (Tübingen ...
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bioRxiv - Genetics 2020Quote: ... Barcoded products were pooled and gel purified from a 1% agarose gel using a QIAgen kit (#28115) to remove primers before quantitation with a Qubit dsDNA HS Kit (ThermoFisher Scientific #Q32851). Samples were loaded onto an Illumina MiSeq instrument with a 300 cycle v2 kit for sequencing ...
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bioRxiv - Microbiology 2020Quote: ... 40 μg protein were mixed with loading dye and loaded on a Bolt™ 4-12% Bis-Tris Plus Gels (ThermoFisher Scientific). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified using the QIAquick Gel Extraction Kit after electrophoresis in agarose gel 1% and then cloned with the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) into competent E ...
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bioRxiv - Molecular Biology 2019Quote: ... Ultrapure N,N,N’,N’-tetramethylethylenediamine (TEMED, cat. #15524010) and SYBR™ Gold Nucleic Acid Gel Stain (cat. #S11494) were obtained from Thermo Scientific. The SequaGel-UreaGel System was purchased from National Diagnostics (cat ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was denatured before separation on 1.5 mm 4%–12% NuPAGE Bis-Acrylamide gels with NuPAGE MOPS SDS running buffer and NuPage antioxidant (Thermo Fisher Scientific, MA). Gels were primed for 10 min at 100 V and run for 1 h at 125 V ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The translation into liposomes was done as described previously95 and the output was analyzed by Blue Native PAGE using 2% digitonin and NativePAGE Novex 4-16% Bis-Tris Protein Gel (Thermo Fisher Scientific). For the in vitro mitochondrial protein import ...
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bioRxiv - Immunology 2021Quote: ... RNA from the barcoded cells for each sample was subsequently reverse-transcribed inside gel bead-in-emulsion using Veriti Thermal Cycler (Thermo Fisher Scientific), and all subsequent steps to generate single-cell libraries were performed according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... The concentration of purified protein was determined by Coomassie staining of 14 % gels after SDS-PAGE with BSA standards obtained from the Pierce BCA protein assay kit (23209, Thermo Fisher Scientific). The purified BZLF1 protein was used as a quantitative standard and reference for subsequent Western blot analyses.
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bioRxiv - Pathology 2019Quote: ... Proteins (10 μg) were separated on 10 % (vol/vol) SDS-polyacrylamide gels followed by electrophoretic transfer of protein to nitrocellulose membrane (Thermo Fisher Scientific). Membranes were incubated overnight with antibodies against Smad2/3 (#8685 and #3122 ...
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bioRxiv - Microbiology 2019Quote: ... 3.75% w/v stacking gel) prepared as described elsewhere [72] and run alongside a protein standard (NativeMark Unstained Protein Standard, Thermo Fisher Scientific) at 25 mA for 3 hr ...