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Citations for Thermo Fisher :
8801 - 8850 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... and then the cells were suspended in culture medium composed of 4.5 g/L glucose-Dulbecco’s modified Eagle’s medium (Gibco, Thermo Fisher Scientific K.K., Kanagawa, Japan) supplemented with 15% regular fetal bovine serum (Biowest ...
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bioRxiv - Physiology 2020Quote: ... Thermo Fisher Scientific, Waltham, MA, USA, supplemented with 2 mM L-glutamine, 1000 units/mL of both penicillin and streptomycin, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... 8 µL viral RNA were reverse-transcribed into cDNA with random primers and the SuperScript III First-Strand Synthesis System (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Femurs were removed and marrow flushed out with complete medium (RPMI, 10% fetal calf serum, 10 units/mL penicillin, 10 μg/mL streptomycin and 2 mM L-glutamine, all Gibco, ThermoFisher UK). Single cell suspensions were prepared by passing the cells through a 19G needle ...
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bioRxiv - Immunology 2020Quote: ... 10% fetal calf serum, 10 units/mL penicillin, 10 μg/mL streptomycin and 2 mM L-glutamine, all supplied by Gibco, ThermoFisher UK), with the correct combination of cytokines and neutralizing antibodies as follows ...
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bioRxiv - Genetics 2020Quote: ... 4 μL of purified product was cloned into the pCR-4TOPO vector using the TOPO TA Cloning Kit for Sequencing (ThermoFisher Scientific, USA). In both cases ...
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bioRxiv - Cell Biology 2020Quote: ... The slices were floated in a 37 °C water bath and mounted either on frosted-edge slides for histological staining (Hurst Scientific, Forrestdale, Western Australia) or on poly-L-lysine-coated slides for immunostaining (Thermo Fisher Scientific). The mounted sections were dried overnight at room temperature.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 50 µl of cell supernatant from each well was transferred into a 96-microwell white plate (polystyrene, Nunclon Delta, Nunc, Thermo Fisher Scientific) and served as a measure of basal luminescence levels in each well ...
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bioRxiv - Physiology 2019Quote: HEK293FT cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 2 mM L-Glutamine and 10% fetal bovine serum (all from Thermo fisher Scientific, USA). MS1 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cell Biology 2020Quote: ... the gel bands were incubated in 150 μL of 100% ACN for 5 minutes at RT and dried for 5 minutes in a SpeedVac Vacuum Concentrator (Thermo Fisher Scientific). Ingel digestion was performed overnight in 50 μL reactions containing 12.5 ng/μL trypsin in 50 mM TEAB at 30°C ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated for 1 h with an F(ab’)2-Goat anti-Mouse IgG (H+L) cross-adsorbed secondary antibody conjugated with Alexa Fluor 488 (Invitrogen, Thermo Fisher Scientific) diluted in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... containing macrophage colony-stimulating factor (M-CSF) was generated by culturing L929 cells in RPMI 1640 medium with L-Glutamine (Thermo Fisher Scientific) containing 10 % fetal bovine serum (FBS ...
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bioRxiv - Physiology 2020Quote: ... Sections were then incubated with Alexa Fluor® 488 conjugated goat anti-rat immunoglobulin G (IgG; [H+L]; 1:100, Life Technologies) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: Samples (20 µL containing 10 µg protein) were loaded per well of a NuPage 4-12 % Bis-Tris gradient gels (#NP0336BOX, Thermo Fisher Scientific), and the SDS-Page gel was subsequently transferred to nitrocellulose membranes (#1620115 ...
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bioRxiv - Microbiology 2021Quote: ... were diluted 1:1000 in milliQ water and left to adhere on a poly-L-lysine glass slide (Thermo Scientific, Menzel-Gläser) for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... to p53 (L-003329-00) or non-targeting control (D-001810-10-05) were transfected into cells with Lipofectamine RNAiMAX (Thermo Fisher Scientific). For TopBP1 transfection ...
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bioRxiv - Genomics 2020Quote: ... cells were maintained at 37°C with 5% CO2 in D10 media: DMEM with high glucose and stabilized L-glutamine (Caisson DML23) supplemented with 10% fetal bovine serum (Thermo Fisher 16000044). K562 cells were maintained at 37°C with 5% CO2 in R10 media ...
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bioRxiv - Biochemistry 2021Quote: ... 4 µl of resuspended MTG1-3xFLAG tagged mitoribosome sample was directly applied onto the grid mounted in the Vitrobot chamber (Thermo Fisher Scientific) and incubated for 1 min ...
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bioRxiv - Plant Biology 2021Quote: ... Eight reactions with 5 μL mRNA each and a GFP construct-specific primer were prepared for cDNA synthesis using SuperScript IV reverse transcriptase (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant bacmid containing hPIV3 L-P genes was generated and isolated following the instruction manual of the bac-to-bac baculovirus expression system (Thermo Fisher Scientific). Viral stocks generated from purified bacmids were amplified and used for protein expression ...
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bioRxiv - Neuroscience 2020Quote: ... Totally around 50,000 cells were plated as thin-layer culture onto poly-L-ornithine coated circular cover glasses (9 mm of diameter, Thermo Fisher Scientific). Co-cultures were cultured 5 weeks in neurosphere medium (NSM) ...
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bioRxiv - Microbiology 2020Quote: ... spun at 200 × g for 10 min and resuspended in macrophage medium (DMEM [Invitrogen] plus 10% FCS and 2 mM L-glutamine) in order to have 2.5 × 106 cells per well ...
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bioRxiv - Microbiology 2020Quote: Metabolic labelling and visualisation were done using Click-iT® AHA (L-azidohomoalaine) and the Click-iT™ Cell Reaction Buffer Kit (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... One μL of the diluted libraries were used to quantify as recommended in the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) with standard range from 0 to 14 ng/ well ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were transfected with 2.5 µM ON-TARGET plus human SAMHD1 siRNA SMART-pool obtained from Dharmacon (Munich, Germany, L-013950-01-0050) in resuspension electroporation buffer R (Invitrogen, Dreieich, Germany) using the Neon transfection system (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... To induce differentiation the cells were seeded into 24-well plates at a density of 2.5 x 104 cells/well and then shifted to DMEM with low glucose (1 g/L; Gibco, Life Technologies, cat. # 11885076) with the same FCS concentration and supplements used for BM-derived SSC differentiation.
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bioRxiv - Genomics 2021Quote: ... K562 cells were acquired from ATCC (CCL-243) and were maintained at 37°C with 5% CO2 in R10 media: RPMI with stabilized L-glutamine (Thermo Fisher 11875119) supplemented with 10% fetal bovine serum (Thermo Fisher 16000044) ...
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bioRxiv - Genomics 2021Quote: ... cells were maintained at 37°C with 5% CO2 in D10 media: DMEM with high glucose and stabilized L-glutamine (Caisson DML23) supplemented with 10% fetal bovine serum (Thermo Fisher 16000044). K562 cells were acquired from ATCC (CCL-243 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... An approximate estimation of concentration in ng •l-1 and purity (260/280 and 260/230 ratios) of each DNA sample was assessed with a NanoDrop (Thermo Scientific, U.S.). The mtMutS region was targeted using the protocols described in the literature [20].
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed three times with PBS-T and incubated with Goat Anti-Mouse IgG (H+L) HRP Conjugate (Thermo Fisher Scientific), Goat Anti-Rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: Secondary antibodies used for tri-functional linker conjugation in LR-ExM were: Goat anti-Guinea Pig IgG (H+L) unconjugated secondary antibody (Invitrogen A18771, RRID:AB_2535548), Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... Wells were coated with 100 μL of 20 μg/mL porcine fibronectin (prepared from whole blood) diluted in PBS at 37°C for 30 min and washed 3x using L-15 media (Gibco, 21083-027). Next ...
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bioRxiv - Physiology 2021Quote: ... they were plated on Poly-L-Ornithine (PLO)-laminin-coated coverslips and differentiated for the first seven days with a formula containing: neurobasal medium (Gibco, Cat # 21103049), 1X B27 plus supplement (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... and HT-29 (HTB-38) were obtained from ATCC (Manassas, VA, USA) and cultured in Leibovitz’s L-15 Medium (11415064, Thermo Fisher Scientific/Gibco) supplemented with 10% FCS at 37°C without CO2 ...
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bioRxiv - Microbiology 2020Quote: ... BHK21 and Vero R cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) 4.5 g/L glucose (Gibco, Thermo Fisher Scientific Inc.) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2021Quote: ... was diluted in a final volume of 220 μL of Sf900 III medium and then combined with a mix of 10 μL of XtremeGene (Cellfectin™ II Reagent, Gibco #10362100) in 100 μL Sf900 III medium ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 2 mM L-Glutamine, 100 nM Dexamethasone, 10% Fetal Bovine Serum (FBS, life technologies, #10270-106) and 1% penicillin-streptomycin (PenStrep, Life Technologies, #15140122). After 4 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... L-013571-00-0005), or siERBB2 (Dharmacon, L-003126-00-0005) SMARTpool siRNA was transfected per well using Lipofectamine™ RNAiMAX (Thermofisher, 13778150) as per the manufacturer’s instructions and incubated for 72 hours ...
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bioRxiv - Plant Biology 2021Quote: ... five-day old seedlings from the indicated genotypes were placed for 3 h in a 2.5 L AnaeroGenTM chamber equipped with an OxoidTM bag to generate hypoxic conditions (Thermo Scientific, Waltham, MA). After the treatment ...
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bioRxiv - Biochemistry 2021Quote: ... SILEC media was prepared by the addition 15N113C3-pantothenate (Isosciences) (1 mg/L) to custom pantothenate free DMEM (Gibco, Thermo Fisher Scientific) containing 25 mM glucose and 4 mM glutamine with all other components at concentrations according to the standard formulation (Dulbecco and Freeman ...
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bioRxiv - Biochemistry 2021Quote: ... SILEC media was prepared by the addition 15N113C3-pantothenate (Isosciences) (1 mg/L) to custom pantothenate free DMEM (Gibco, Thermo Fisher Scientific) containing 25 mM glucose and 4 mM glutamine with all other components at concentrations according to the standard formulation (Dulbecco and Freeman ...
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Natural abundance isotope ratios to differentiate sources of carbon used during tumor growth in vivobioRxiv - Cancer Biology 2020Quote: ... a white rice obtained from a supermarket and homogenized with a ball mill, δ13C = -27.43‰ ; and “JGLUT”, L-glutamic acid obtained from Fisher Scientific, δ13C = -13.43‰) were incorporated into each analytical batch run and used to normalize the data to the Vienna Pee Dee Belemnite (VPDB ...
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bioRxiv - Biophysics 2020Quote: ... overnight at 37°C and then used to inoculate 1 L of fresh LB and 10 μg/mL kanamycin (Fisher Scientific, BP906). Growth of cells were performed at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was performed using 2.5 µL of cDNA as template with attB-flanked gene-specific primers (Supplementary Table S5) in a Veriti Thermo Cycler (Thermo Fisher Scientific) using Phusion polymerase master mix (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed with PBS and blocked with R10 media (RPMI-1640 with 10% FCS, 1% Pen/Strep, 2mM L-Glutamine (all from Thermo Fisher Scientific)) for 30 minutes at RT before the addition of freshly isolated PBMCs ...
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bioRxiv - Microbiology 2021Quote: ... 500 µL of high titer stocks (∼1010 pfu/mL) of wild-type of mutant T7 were treated with TURBO DNAse (Thermo Fisher Scientific) for 30 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were grown at 20 °C for 72h in 160 μl 1 g l−1 TSB in 96-well microtiter plate with TSP lid (TSP, NUNC, Roskilde, Denmark). Planktonic cells were removed by immersing the lid with pegs three times in phosphate-buffered saline solution (PBS) ...
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bioRxiv - Microbiology 2020Quote: ... we use 1µg/ml final concentration of ethidium bromide to stain parasitized erythrocytes and 4 µl/ml Alexa Fluor 488-conjugated goat anti-human IgG (H+L) (Invitrogen, Life Technologies), based on optimal signal to noise between positive control plasma (10 Mali highly exposed individual plasma ...
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bioRxiv - Immunology 2021Quote: DuckCelt®-T17 cells in a working volume of 10mL in TubeSpin® 50 mL or 500 mL in 1 L Erlenmeyer shaker flasks were inoculated with HMPV in OptiPRO™ SFM (Gibco) supplemented with 1% penicillin/streptomycin (Gibco) ...