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Citations for Thermo Fisher :
8751 - 8800 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... DNA quality was assessed on a 1% agarose gel and quantified using the Quant-IT dsDNA Broad Range Kit (Thermo Fisher Scientific). Genomic DNA was sheared using a Covaris LE220 focused-ultrasonicator to a 450 bp mean insert size.
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bioRxiv - Cell Biology 2021Quote: ... and Reducing Agent (NuPAGE™ Sample Reducing Agent (10X) Invitrogen NP0004) at 70°C for 10 minutes before loading in NuPAGETM 4-12% Bis-Tris Gels (Invitrogen NuPAGE NP0336). 1x MOPS (BOLT Invitrogen B000102 ...
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bioRxiv - Physiology 2021Quote: ... aliquots of 30µg of protein were prepared in native Tris glycine sample buffer and analysed from NuPAGE 3-8% Tris-Acetate polyacrylamide gel electrophoresis in a tris-glycine native running buffer (Thermo Fisher Scientific). After 2-hours of migration at 130 V ...
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bioRxiv - Cell Biology 2021Quote: ... Lphn2a-/- heterozygous and homozygous mutants were identified by analyzing in agarose gel the PCR product digested with TasI restriction enzyme (TSP509I, Thermo Fisher Scientific) and by sequencing.
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bioRxiv - Cell Biology 2021Quote: In-gel digested samples were analyzed on a Dionex Ultimate 3000 nano-UHPLC coupled to a Q Exactive mass spectrometer (Thermo Scientific, Bremen). The samples were washed on a trap column (Acclaim Pepmap 100 C18 ...
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bioRxiv - Plant Biology 2021Quote: ... 2 μg of EV and OM samples and 20 μg of WC samples were loaded on a NuPAGE 4-12% Bis-Tris Protein gels (Thermofisher Scientific, US), and the gels were run for 3 min only ...
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bioRxiv - Synthetic Biology 2021Quote: ... Colony PCR products were versified on 1% agarose gels stained with RedSafe™ (ChemBio) and then purified with GeneJET PCR purification kit (Thermo Scientific) and sequenced by SourceBioscience.
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bioRxiv - Microbiology 2020Quote: ... 20 μl of antibody-conjugated beads/resin (FLAG: EZview Red ANTI-FLAG M2 Affinity Gel, Sigma-Aldrich; HA: Pierce Anti-HA Agarose, Thermo Scientific) for 30-60 in at 4°C with rotation ...
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bioRxiv - Plant Biology 2020Quote: ... The electrophoresis of amplified products was performed on 6% acrylamide gels at 160 Volts for 30 minutes (Thermo Electron Electrophoresis EC250-90, Thermo Fisher Scientific). The results were visualized under UV light using UVITEC Imaging Systems (Cleaver Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The resulting PCR product was purified using the QIAquick Gel Extraction Kit and then cloned with the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) into competent E ...
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bioRxiv - Microbiology 2021Quote: ... 10 µg of the whole cell lysate was separated using NuPAGE 4−12% Bis-Tris gradient gels (Invitrogen Life Sciences, Carlsbad, CA) and transferred to a PVDF membrane (Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... EV lysates corresponding to 5–10 µg of total protein were run into 4-12% Bis-Tris plus gels (Invitrogen, ThermoFisher Scientific). Prior to loading ...
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bioRxiv - Plant Biology 2021Quote: ... Quality of the purified PCR products was checked on a 1% agarose gel and then quantified spectrophotometrically using a NanoDrop 1000 (Thermo Fisher Scientific). The libraries were pooled using an equimolar ratio ...
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bioRxiv - Molecular Biology 2020Quote: ... A fraction of the purified proteins was separated by SDS-PAGE on a 4-12% NuPAGE Novex Bis-Tris precast gel (Thermo Fisher Scientific) and visualized by silver staining and western blot analysis with the indicated antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... The biotinylated DNA fragments were applied to the gel-shift assay by using the LightShift Chemiluminescent EMSA Kit (Thermo Fisher Scientific, US) and Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... each of the libraries was diluted 4-fold prior to running on a 1% Agarose E-gel (Life Technologies, #G402001, Carlsbad, CA) with a E-Gel 1-Kb Plus DNA Ladder (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: Total proteins were prepared by direct lysis of 2 million of SiHa cells in 350 ul of 4× SDS-sample buffer and 15 ul of the sample was separated in a NuPAGE Bis-Tris 4– 12% gel (Thermo Fisher Scientific) in 1× MOPS SDS buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: 10 ul whole cell extracts of 2 × 105 cells in 40 μl 6X SDS loading buffer were run on 4-14% bis-tris gel (Invitrogen cat # NP0335). Membranes were transferred by semi-dry apparatus (Bio-Rad Transblot cat # 170-3940 ...
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bioRxiv - Cancer Biology 2020Quote: Ten to thirty micrograms of proteins were loaded on to pre-casted SDS-PAGE gels (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and transferred to a PVDF membrane (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... 100-120 µg of total protein were run on Invitrogen NuPAGE 4-12% precast Bis-Tris polyacrylamide gels for SDS-Page (Thermo Fisher Scientific) and then transferred onto nitrocellulose membranes for Western Blotting ...
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bioRxiv - Biophysics 2021Quote: ... Fractions were collected as shown in Figure 4C and the amount of recovered protein was determined by SDS-PAGE (Nu-PAPGE 12% Bis-tris gel, Thermo Fisher Scientific) stained with SYPROTM Orange (Invitrogen).
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bioRxiv - Biophysics 2020Quote: ... To determine the point of elution of aSyn from the chromatography column protein fractions which were collected and monitored on absorption at 280 nm were ran on a 4-12% Bis-Tris gel (Invitrogen, Thermo Fisher) using SDS-PAGE and stained with Coomassie blue ...
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miR-206 inhibits estrogen-induced proliferation and invasion of ER-α36 positive gastric cancer cellsbioRxiv - Cancer Biology 2021Quote: ... 60 μg protein was separated with 12% SDS-PAGE gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Thermo Fisher Scientific, Inc.). The PVDF membrane was sealed with 5% skim milk powder in the PBS containing tween (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant 30 μL was loaded into each well of 4-20 % or 12% Tris-glycine Novex™ WedgeWell™ precast gel (Invitrogen). Equal quantities of protein samples were loaded in all the wells of each individual gel ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μg of protein samples were subjected to SDS-PAGE on a 10% polyacrylamide gel followed by Western blotting using an iBlot 2 Dry Blotting System (Thermo Fisher Scientific). 1.0 μg/ml each of rabbit polyclonal anti-nucleolin antibody ...
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bioRxiv - Cell Biology 2021Quote: ... 40 µg of total RNA isolated from mESC were separated with agarose electrophoresis with RNA Gel Loading Dye (2X) (Thermo, R0641) and NorthernMax™ 10X Running Buffer (Ambion, AM8671). The separated total RNA samples were further transblotted to positively charged nylon transfer membrane (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified by centrifugation and were resolved on a 15-well NuPAGE 4-12% Bis-Tris protein gel (Thermo Fisher Scientific) prior to transfer onto nitrocellulose membranes ...
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bioRxiv - Microbiology 2021Quote: 5 - 10μg of protein from the cell lysates were run on 4-12% NuPAGE Bis-Tris SDS-PAGE gels (Invitrogen by ThermoFisher Scientific®) and transferred to PVDF membranes (ThermoFisher Scientific® ...
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bioRxiv - Genomics 2019Quote: ... Total RNA quality and quantity was assessed with gel electrophoresis and a Broad Range RNA Qubit assay (Thermo Fisher Scientific, Waltham, MA). Illumina sequence libraries were prepared using the NEB Next Ultra Directional RNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... were separated using NuPAGE Bis-Tris gels (4 – 12 %) with 3-(N-morpholino)propanesulfonic acid (MOPS) or 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (Thermo Fisher Scientific) followed by transferring to polyvinylidene difluoride (PVDF ...
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bioRxiv - Genomics 2022Quote: ... We used 1ul for quantification with Qubit (Oxford Nanopore Technologies) and 1ul for determining the DNA size with an E-gel EX cartridge (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... or 30μg (cell lines) of protein lysates were separated on Tris-Glycine (TGX) precast gels (BIO RAD) and transferred to nitrocellulose membranes (Thermo Fisher Scientific). Membranes were probed overnight with antibodies to FoxA1 (1:1000 ...
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bioRxiv - Bioengineering 2022Quote: ... 40 µg total protein from planarian lysate or 5 µg total protein from HeLa cell lysate were loaded per well onto a NuPAGE 4-20 % BisTris gel (ThermoFisher, Cat#NP0321BOX) and run in 1× MOPS buffer (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: Samples were resolved on 4-12% Bis-Tris NuPAGE gel at constant voltage (120V) using NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific) and transferred to nitrocellulose membrane in NuPAGE transfer buffer using BioRad Protean wet-transfer system at constant voltage (100V ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were resolved by electrophoresis at 150 V for 3 h at room temperature in Novex TBE-Urea Gels (EC6885BOX, Thermo Fisher Scientific) in 1x TBE buffer and cleaved and uncleaved products were imaged on ChemiDoc (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... 30 μg from each sample were run on 4-12% SDS gels and transferred to nitrocellulose membranes with the iBlot® dry blotting system (Life Technologies) for 7 min ...
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bioRxiv - Neuroscience 2022Quote: ... and protein levels resolved using 1x LI-COR Protein Loading Buffer (Li-Cor #928-40004) loaded into a 4-20% SDS-PAGE gradient gel (ThermoFisher Scientific #26224) and separated for 2h at room temperature in 1x SDS running buffer (Pierce 20x Tris Hepes SDS Buffer #28368 ...
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bioRxiv - Microbiology 2022Quote: ... China).The purity of the protein was assessed by SDS-PAGE gel and its concentration was determined using the BCA Protein Assay Reagent (Thermo Fisher Scientific). AdpA cysteine mutant proteins (C62S ...
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bioRxiv - Plant Biology 2022Quote: ... the amplified fragments between 250-300 bp were specified on the E-gel system and sent for sequencing on an Ion Proton sequencer (Life Technologies, Inc.). The sequencing data were first trimmed to 64 bp ...
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bioRxiv - Neuroscience 2022Quote: ... 50-75 μg of soluble protein per sample (same for all samples in a set) was run on 10% Bis-Tris Bolt PAGE gels (Fisher Scientific, #NW00100BOX), and wet-transferred to nitrocellulose membrane (Protran 0.2 μm NC ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were killed with 10 μL of 6X SDS loading buffer and pooled or directly loaded onto a 15-well Novex Wedgewell 8% Tris-Glycine Gel (Thermo Fisher Scientific) and ran at 175 V for ~ 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were subjected to SDS-PAGE electrophoresis using NuPAGE 4-12% Bis-Tris gels and MES running buffer (Thermo Fisher Scientific) according the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... All purified PCR products were visually checked for expected size via 1% agarose gel electrophoresis and assessed for purity and yield via Nanodrop (Thermo Fisher Scientific).
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bioRxiv - Genetics 2022Quote: ... Subsequent nested PCRs were performed to generate amplicons for NGS and the second PCR product was purified using GeneJET Gel Extraction Kit (Thermo Fisher Scientific). Furthermore ...
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bioRxiv - Molecular Biology 2022Quote: ... (all the primer sequences are listed in Table 1 and the PCR conditions are listed in Table 2) respectively.The PCR product were purified using Quick gel extraction kit (Invitrogen: Cat no. # K210012). The purified double-stranded 5′UTR fragment and 3′UTR fragment (containing overhangs for insert and vector fragment ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were denatured for 1 minute at 70°C and RNA-protein complexes were resolved on NuPAGE 4-12% Bis-Tris Gels (Thermo Fisher, #WG1402A) at 180V for 1 hour ...
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bioRxiv - Biophysics 2022Quote: Blue Native-PAGE was performed as descripted before (38) with the NativePAGE™ Bis-Tris Gel System (Life Technologies, Carlsbad, CA, USA). Total membranes of E ...
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bioRxiv - Biochemistry 2022Quote: ... a total of 120 μg of protein from lysates was migrated on a pre-cast 4-12% Novex NuPAGE SDS Mini Gel (Invitrogen/Thermo Fisher) using the Bis/Tris buffer system (product number NP0326BOX ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The quality and size of the precursor was checked on agarose gel and its concentration was measured using Qubit RNA BR (Broad Range) Assay Kit with the Qubit Fluorometer (Thermo Fisher Scientific). The concentration ranged from 1500 ng/μl to 2000 ng/μl.
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bioRxiv - Cell Biology 2022Quote: ... 10 μL of sample (which is the equivalent of 10 embryos) was loaded into each lane of a 3-8% Tris-Acetate pre-cast SDS-PAGE gel (Invitrogen, Thermo Fisher Scientific) and afterwards transferred from the gel onto a nitrocellulose membrane (BIO-RAD ...