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Citations for Thermo Fisher :
8751 - 8800 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Leaves discs were allowed to recover overnight in water and assayed in 20 µg/mL HRP and 6 µM L-012 in a 96-well luminometer plate (Thermo Fisher Scientific), with or without 100 nm flg22 ...
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bioRxiv - Developmental Biology 2022Quote: ... Xeno-free media (XFM-) was prepared using 500 mL of RPMI 1640 medium without L-Glutamine (Thermo Fisher Scientific, Canada. Catalog# 11875101), 10mL B-27 Supplement minus insulin (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... and the secondary antibody reaction was performed using goat anti-mouse IgG (H+L) (Alexa Fluor Plus 555; Invitrogen, Waltham, MA, USA) at 1:200 dilution with 1% BSA-PBS for 40 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... originally obtained from ATCC) were cultured at 37 °C and 5% CO2 in DMEM (high glucose 4.5 g/l; Gibco, Thermo Fisher cat# 10566024) supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... ATCC TIB-202) or primary human monocytes (PHMs) were propagated in RPMI 1640 with L-glutamine and 25 mM HEPES buffer (Invitrogen, Carlsbad, CA), supplemented with 1 mM sodium pyruvate (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... adherent cells were maintained in complete DMEM media (Dulbecco’s Modified Eagle Medium supplemented with 10% FBS, 2 mM L-Glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin – all from Life Technologies, Gibco). Cells were routinely detached with trypsin EDTA and passaged 1 in 10.
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bioRxiv - Microbiology 2022Quote: Biotinylation of HBs-S and HBs-L was performed using the Antibody Biotinylation Kit for IP (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2022Quote: ... PC9 cells were maintained in Roswell Park Memorial Institute (RPMI) medium with L-glutamine (Nacalai Tesque) supplemented with 10% FBS (Thermo Fisher Scientific) and 1% P/S ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells expressing ACE2 were seeded at 1.5×104 cells/well in poly-L-Lysine (0.01%) coated 96-well black plates (Thermo Fisher Scientific, Cat# 3904) one day before titration ...
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bioRxiv - Immunology 2020Quote: Cloned mAb vectors for IgG1 heavy chain and Kappa or Lambda light chains were co-transfected at a ratio of 1:3 (H:K/L) into Expi293F cells (Thermo Fisher Scientific Inc.) using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific Inc.) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were probed with goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody linked to Alexa Fluor 647 (Thermo Fisher Scientific) (0.6 μl/100 μl per tube ...
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bioRxiv - Microbiology 2021Quote: ... Samples were injected in randomized order at a volume of 10 μL and eluted using a polar to apolar gradient formed using Ultrapure water (A) and LC/MS grade acetonitrile (Thermo Fisher Scientific) (B) ...
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bioRxiv - Cell Biology 2019Quote: ... with TBST and then incubated for 1-1.5 h at room temperature in the darkness with a goat polyclonal anti-rabbit IgG (H+L) DyLight 800 4X PEG (Invitrogen, SA5-35571) diluted (1:10,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Gels were run at 100 V using 1 X SDS-PAGE Running Buffer [100 mL of 10 × 1 L Stock [30.2 g Tris Base (Fisher Scientific, BP152-5), 188 g Glycine (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: Each dissected brain sample was digested with 8 µl of protease in 200 µl of digestion buffer from the RecoverAllTM Total Nucleic Acid Isolation Kit for FFPE (Thermo Fisher Scientific). Samples containing big chunks of brain tissue were homogenized in an electronic homogenizer ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown at 37°C / 7.5% CO2 in DMEM medium containing 4.5 g/l glucose (Life Technologies, Thermo Fisher Scientific, Germany) and 10% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown at 37°C / 7.5% CO2 in DMEM medium containing 4.5 g/l glucose (Life Technologies, Thermo Fisher Scientific, Germany) and 10% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed in 10% formamide and 2× SSC and adhered to 0.01% poly-L-lysine–coated coverslips before mounting in ProLong diamond antifade mounting solution with DAPI (Life Technologies Cat# P36970).
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bioRxiv - Microbiology 2021Quote: ... 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin, Vector Laboratories, #L-1060 + DyLight755 Antibody Labelling Kit, Thermofisher Scientific, #84538), 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31 ...
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bioRxiv - Microbiology 2021Quote: ... 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin, Vector Laboratories, #L-1060 + DyLight755 Antibody Labelling Kit, Thermofisher Scientific, #84538) and 50 μg of Hydrazide AlexaFluor633 were injected in the mouse tail vein to label and visualize the human vessels and the arteriole walls ...
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bioRxiv - Microbiology 2021Quote: ... 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin, Vector Laboratories, #L-1060 + DyLight755 Antibody Labelling Kit, Thermofisher Scientific, #84538) were injected in the mouse tail vein to label and visualize the human vessels ...
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bioRxiv - Microbiology 2021Quote: ... and the human vasculature with 100μg of Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin, Vector Laboratories, #L-1060 + DyLight755 Antibody Labelling Kit, Thermofisher Scientific, #84538). Neutrophils were labelled with 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G ...
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bioRxiv - Microbiology 2020Quote: TSA assays were performed by mixing 5 μL protein solution at 2 mg/mL with 5 μL SYPRO®Orange (1:50 diluted from 5000x stock solution, Invitrogen, USA), 5 μl of 8-fold buffer (1200 mM NaCl and 400 mM Tris pH 8) ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were kept at 37°C and 5% CO2 in neurobasal medium containing 2 mM L-glutamine and B27 supplement (Thermo Fisher Scientific) with an exchange of half the medium after 4 days in culture.
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bioRxiv - Microbiology 2021Quote: ... The next day samples were washed 3 times with 1X PBS and incubated with secondary antibody (goat anti- mouse IgG H+L Alexa Fluor 594 (Invitrogen, A-11032)) diluted in blocking buffer for 1 hr at room temperature in the dark ...
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bioRxiv - Microbiology 2021Quote: ... 0.12 U μl−1 Turbo DNase (Albion) or 5 U RNase A and 20 U RNase T1 (RNase Cocktail enzyme mix, Thermo Fisher Scientific) for 1 h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells of 5 mL culture were incubated for 15 min with 2.5% glutaraldehyde and pre-fixed for 2 h on poly-L-lysine coated coverslides (12 mm, Fisher Scientific, Schwerte, Germany). Cover slides were washed three times with 0.1 M sodium cacodylate (pH 7.2 ...
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bioRxiv - Cancer Biology 2020Quote: ... 100nM of siRNA non-targeting control (siRNA NTC, D-001810-10-05) or siRNA SOX11 (L-017377-01-0005, Dharmacon) were transiently transfected using DharmaFect 2 (Thermo Fisher Scientific) according to the manufacturer's guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... AP Rabbit anti-Goat IgG (H+L) secondary antibody and 1-Step NBT-BCIP substrate were purchased from Thermo Fisher (Waltham, MA). The CellTiter 96® AQueous One Solution Cell Proliferation Assay was purchased form Promega (Madison ...
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bioRxiv - Neuroscience 2020Quote: Ba/F3 cell line was cultured and passaged as described (Cermak et al., 2016).It was maintained in RPMI-1640 media with L-Glutamine (Invitrogen, Cat #11875093) supplemented with 10% FBS ...
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bioRxiv - Biophysics 2020Quote: ... Alexa Fluor 647 F(ab’)2 of goat anti mouse IgG (A-21235, Thermo Fisher, Al532-Goat anti Rabbit IgG (H+L) (A-11009 ...
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bioRxiv - Biophysics 2020Quote: ... Alexa Fluor 647 F(ab’)2 of goat anti mouse IgG (A-21235, Thermo Fisher, Al532-Goat anti Rabbit IgG (H+L) (A-11009, Thermo Fisher), Al532-Goat anti Rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were then treated for 60-90 min with a secondary antibody solution containing goat anti-guinea-pig IgG (H+L) antibody conjugated with Alexa Fluor 594 (A-11076; Thermo Fisher Scientific) (1,000× dilution) ...
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bioRxiv - Cancer Biology 2021Quote: ... Transient transfections of SMARTPool SGK3 siRNA (L-004162-00-0005, Horizon Discovery) were performed with Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... Ixodes ricinus (IRE/CTVM19) cell lines were maintained at 28 °C in Leibovitz L-15 medium (Gibco, Thermo Fisher Scientific, Waltham, USA) supplemented with 10% tryptose phosphate broth (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... by using non targeting (D-001810-10-20) or TAZ (L-016083-00-0005) ON-TARGETplus SMARTpools and Lipofectamine RNAimax (Invitrogen 13778-075).
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bioRxiv - Immunology 2022Quote: ... and donkey anti-rabbit IgG (H + L) highly cross-adsorbed secondary antibody conjugated with Alexa Fluor 594 (A-21207, Thermo Fisher Scientific). The mouse monoclonal anti-HCV core antibody was a gift from Chen Liu.
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bioRxiv - Genomics 2022Quote: ... Hutchinson-Gilford Progeria Syndrome (HGPS) fibroblasts were purchased from the Progeria Research Foundation and cultured in high glucose DMEM without L-glutamine (ThermoFisher, 11960-440) supplemented with 20% FBS (Corning ...
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bioRxiv - Cell Biology 2022Quote: ... The bacterial pellets from 1 L of growth media were resuspended in 30 mL of B-PER reagent (Thermo Scientific, Waltham, MA), and expressed proteins were purified from the lysis supernatant by Nickel affinity and gel filtration (Superdex 75 26/60 ...
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bioRxiv - Developmental Biology 2022Quote: ... day 4 aggregates were dissociated into single cells and spread onto poly-L-lysine-treated Superfrost™ Plus Microscope Slides (Fisher Scientific). The slides were fixed in 4% paraformaldehyde in PBS for 15 min at room temperature (RT) ...
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bioRxiv - Cancer Biology 2022Quote: ... was diluted in T cell medium (RPMI 1640 + 10%FBS + L-glutamine (2 mM) + penicillin (100 U/ml + streptomycin (100 μg/ml) + 55 μM 2-Mercaptoethanol (Thermo Fisher, #21985023)) ...
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bioRxiv - Cell Biology 2022Quote: ... first on solid YE4S agar and then in liquid Edinburgh Minimal Media supplemented with 20.0 g/L Dextrose Anhydrous (Fisher Scientific BP350-1) and 5.0 g/L Ammonium Chloride (Sigma A9434 ...
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Purkinje cardiomyocytes of the ventricular conduction system are highly diploid but not regenerativebioRxiv - Developmental Biology 2022Quote: ... heart tissue sections were incubated at room temperature for 2 hr with secondary antibodies: goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody AlexaFluor 488 conjugate 1:500 (A11001, Thermo Fisher Scientific); goat anti-rabbit IgG (H+L ...
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bioRxiv - Immunology 2019Quote: ... paraffin embedded DI sections (4 µm) were mounted on poly-L-lysine-coated glass slides (Superfrost Plus, Thermo Fisher scientific, Braunschweig, Germany) and left to dry at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... while for the ECAR measurements the media was supplemented with 1 mM of Sodium Pyruvate and 2 mM of L-glutamine (both from Life Technologies, UK), pH 7.4 at 37ºC.
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bioRxiv - Immunology 2020Quote: ... 10% fetal calf serum, 10 units/mL penicillin, 10 μg/mL streptomycin and 2 mM L-glutamine, all supplied by Gibco, ThermoFisher UK). Cytokines added were ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... RNA was eluted in 30 µl RNAse free water and RNA concentration was determined by measuring absorbance at 260 nm using a Nanodrop one (Thermo Fisher Scientific).
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bioRxiv - Immunology 2019Quote: ... Secondary antibodies against mouse IgG (H+L) F(ab’)2 fragment conjugated to AlexaFluor 647 or FITC (Molecular Probes, Thermo Fisher Scientific) was used for the detection of PTPN2 ...
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bioRxiv - Immunology 2019Quote: ... Secondary antibodies against mouse IgG (H+L) F(ab’)2 fragment conjugated to AlexaFluor 647 or FITC (Molecular Probes, Thermo Fisher Scientific) was used for the detection of PTPN2 ...
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bioRxiv - Cancer Biology 2019Quote: ... containing 4.5 g/L glucose, supplemented with 10% fetal bovine serum (FBS, Silantes, Munich, Germany) and 1% penicillin–streptomycin–neomycin (Invitrogen, Carlsbad, CA) at 37 °C in a humidified atmosphere of 5% CO2.