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Citations for Thermo Fisher :
8701 - 8750 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... The DNA purity was determined by 0.8% agarose gel 100 V electrophoresis for 40 min and DNA concentration was determined by Qubit® 2.0 fluorescent meter (Invitrogen, Carlsbad, USA). Following quality assessment ...
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bioRxiv - Biochemistry 2020Quote: ... In-gel digestion was performed as previously described.31 Digested samples were desalted using Pierce™ C18 Spin Tips (Thermo Scientific #84850) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2020Quote: ... The quality and quantity (concentration) of total DNA were determined by 1% agarose gels and a NanoDrop 2000 spectrophotometer (Thermo Scientific, USA), respectively.
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bioRxiv - Immunology 2019Quote: ... to ensure equal amounts of total protein were loaded in each well of NuPAGE 4–12% Bis-Tris Gel (Thermo Fisher Scientific). Proteins were transferred onto a nitrocellulose membrane and blotted with the appropriate antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... Target protein bands detection was done in Gel Documentation system (Syngene: chemiXX9) using Super signal West Pico Chemiluminescent substrate (Thermo Scientific, #34077) and densitometric quantitation assessed using Image Studio Lite v5.2 (LI-COR Biosciences).
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bioRxiv - Neuroscience 2019Quote: ... 5μg of protein from synaptoneurosome fractions and molecular weight marker (Li-Cor, Cambridge, UK) was loaded onto NuPAGE 4-12% Bis-Tris precast polyacrylamide 15 well gels (Invitrogen, Paisley, UK). Proteins were transferred to polyvinylidene fluoride (PDVF ...
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bioRxiv - Molecular Biology 2019Quote: ... 13 µL of each sample was electrophoresed on a 4%–12% Bis-Tris protein gel (NuPAGE Novex, (ThermoFisher Scientific, Waltham, MA, USA) for 35 minutes at 200 V using MOPS buffer in a PowerPac™ HC Power Supply (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... 20 μg of total protein was separated on NuPAGE® 4-12% Bis-Tris gels using MES buffer and the XCell sure-lock mini-cell system (ThermoFisher Scientific), transferred to a polyvinylidene difluoride membrane(PVDF ...
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bioRxiv - Microbiology 2020Quote: ... For gel electrophoresis we transferred 5 μl of the PCR reaction to a 1.5% agarose gel stained with SYBR Safe (Invitrogen, Thermo F. Scientific) and visualized by UV illumination ...
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bioRxiv - Neuroscience 2019Quote: ... while nine tenth of the samples were analyzed via Coomassie staining after separation on 7% NuPAGE™ Tris-Acetate protein gels (Thermo Fisher) in NuPAGE™ Tris-Acetate SDS running buffer (Thermo Fisher) ...
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bioRxiv - Immunology 2020Quote: ... Size-based protein separation was achieved by use of 4-12% SDS PAGE gels (Novex) and MES-running buffer (Thermo Fisher Scientific). Proteins were then transferred onto a PVDF membrane (Immobilon®-P Transfer Membrane ...
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bioRxiv - Biochemistry 2019Quote: ... proteinase K was added to the samples for 1 hours and DNA was extracted by phenol/chloroform and phenol protocol and ran on a 10% Novex TBE gel (ThermoFisher cat #EC6275BOX). The gel was post-stained with Syto 60 (Invitrogen cat #S11342 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein samples were heat-denatured at 98°C for 8 minutes in denaturing Laemmli buffer (containing 2-mercaptoethanol) and resolved on 4–12% gradient Invitrogen Bolt Bis-Tris Plus gels (Thermo Fisher Scientific). The resolved proteins were transferred via wet transfer in buffer (1X Tris-glycine ...
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bioRxiv - Physiology 2019Quote: ... Approximately 20⍰ μg protein from each sample was electrophoresed on a NuPage 4–12% Bis-Tris gel and blotted onto nitrocellulose membrane using the Novex system (Thermo Fisher Scientific). Blots were stained with Ponceau S and probed with IgG purified rabbit anti-Drip/Prip/Eglp2/Eglp4 antibodies (1 μg mL−1 ...
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bioRxiv - Developmental Biology 2019Quote: ... An aliquot containing 25μg protein was fractionated on a 4-12% Bis-Tris gel and blotted onto Hybond C-extra nitrocellulose membrane using NuPAGE transfer buffer (Thermo Fisher Scientific) containing antioxidant ...
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bioRxiv - Developmental Biology 2019Quote: ... were fractionated on NuPAGE 4-12% Bis Tris gels in MOPS buffer and blotted onto Hybond C-extra nitrocellulose membrane using NuPAGE transfer buffer (Thermo Fisher Scientific) containing antioxidant ...
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bioRxiv - Genomics 2019Quote: The yield and quality of DNA were assessed by agarose gel electrophoresis and by a NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, Delaware) and quantified by Qubit broad range assay (Thermo Fisher Scientific). Illumina sequencing libraries were prepared ...
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bioRxiv - Genomics 2019Quote: ... 9- and 12-Kbp insert sizes) were prepared using Illumina’s Nextera Mate Pair kit with size selection performed on precast E-gel (Life Technologies, Europe BV) 0.8% agarose gels ...
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bioRxiv - Biochemistry 2019Quote: ... The purified core complex was analyzed by 10 % polyacrylamide SDS-gel electrophoresis and its purity was determined to be 80-90 % by staining with Coomassie Brilliant Blue (Thermo Fisher Scientific). The composition of the core complex was confirmed by immunoblotting with anti-Flag and anti-c-Myc antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... the TFM gels were incubated for 30 minutes at RT with an activation solution [0.2 mg/ml of Sulfo-SANPAH (Thermo Fisher Scientific, 22589), 2 mg/ml N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC ...
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bioRxiv - Bioengineering 2019Quote: ... All samples were run on NativePAGE 4-16% Bis-Tris Gel to separate dCas9 bound gRNAs and unbound gRNAs (Thermo Fisher Scientific). Samples containing only dCas9 ...
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bioRxiv - Biochemistry 2020Quote: ... of HLC protein extract in 4 × Sample Loading Buffer (Li-Cor, 928-40004) were loaded onto NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen, NP0326BOX). Following resolution ...
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bioRxiv - Genetics 2020Quote: ... were added to the samples These were heated at 70°C for 10 minutes and loaded onto 4-12% Bis-Tris gel (Thermo Fisher, NW04125BOX). The proteins were separated with 150V electrophoresis for 30 minutes and blotted onto a PVDF membrane using the iBlot Dry 2 blotting system ...
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bioRxiv - Genetics 2019Quote: ... fractions or equal amounts of protein lysates were separated on either 4-12% or 12% NuPAGE Bis-Tris gels (Thermo Fisher Scientific) depending on the size of the protein of interest and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Genetics 2021Quote: ... The samples were returned as pellets to be re-suspended with 50 ml Elution Buffer before the DNA quality and quantity was assessed using gel electrophoresis and NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Evolutionary Biology 2020Quote: ... We quantified DNA concentration using a Qubit fluorometer and checked quality by running the samples on a 1% agarose gel (Invitrogen; Carlsbad, CA). We sent samples to the Cornell Institute of Biotechnology for library preparation and sequencing ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Quality control of the RNA was performed on agarose gels and the Qubit RNA HS or BR Assay kit (Invitrogen, Carlsbad, CA) for quantification ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated at 80 °C for 10 min and run on NuPAGE Bis-Tris polyacrylamide gels in NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific). The iBlot 2 gel transfer device (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... All dsRNA reagents were verified by running on a 1% agarose gel and quantified by measuring absorbance at 260 nm using a NanoDrop™ (ThermoFisher Scientific).
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bioRxiv - Pathology 2021Quote: ... were separated on 4-12% Bis-Tris gels in MOPS buffer and transferred to 0.45 μm nitrocellulose membranes via semi-dry conditions (iBlot 2, NuPage, Thermo Scientific, Paisley, UK). PageRuler Plus prestained protein ladders (3 μg/lane ...
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA was visualized on a 1% agarose gel and quantified with a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Neuroscience 2021Quote: ... Beads were immediately placed immediately onto a magnetic rack and the entire sample was immediately loaded into 10% Bis-Tris polyacrylamide gels (Invitrogen, ThermoFisher catalog #NP0303BOX) and transferred to PVDF membranes (Immobilon-P ...
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bioRxiv - Bioengineering 2020Quote: ... The gels were subsequently silver stained in clean glass trays using the Pierce Silver Stain kit for mass spectrometry (ThermoFisher Scientific, Inc.). Each gel was developed for approximately 45 seconds prior to halting stain development in 5% acetic acid ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were incubated at 70°C for 10 minutes and resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific) followed by electroblotting onto Immun-Blot PVDF Membrane (Biorad ...
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bioRxiv - Biochemistry 2021Quote: ... Sample purity was assessed via SDS-PAGE gel and working concentrations determined via Pierce 660nm protein assay reagent and BSA standards (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2021Quote: ... Sample purity was assessed via SDS-PAGE gel and working concentrations determined via Pierce 660nm protein assay reagent and BSA standards (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2021Quote: ... and then heated at 95 °C for 5 min before loading onto a pre-cast 4-12 % Bis-Tris polyacrylamide gel (Thermo Fisher Scientific). Samples were run at 200 V for 45 min before staining with Coomassie Blue.
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bioRxiv - Neuroscience 2020Quote: ... 10μg protein was loaded per well on 8% acrylamide gels with prestained standard molecular weight markers (PageRuler prestained protein ladder; Thermo Fisher Scientific) and run at 60mV for 3 hours using the mini Protean II gel electrophoresis apparatus (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2021Quote: ... purified α-Syn monomers were passed through a polymyxin B-conjugated resin (Detoxi-gel endotoxin removing column, Thermo Scientific, Waltham, MA, USA). α-Syn oligomers (Oα-Syn ...
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bioRxiv - Neuroscience 2020Quote: ... To elute samples the beads were then boiled in SDS sample buffer for 5 mins and run on a 4-12% Bis-Tris gel (Invitrogen, Cat# NW04127BOX). Rabbit GluR2 polyclonal antibody (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... and heated for 10 mins at 70 °C (apart from samples meant for detection of amyloid) and run on 4–12% Bis-Tris precast gels (Nupage, Thermo Fisher Scientific) in MOPS buffer (Nupage ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were heated at 95 °C for 5 min and run on a 4-12% Bis-Tris NuPage gel (Thermo Fisher, #NP0321). Samples were then transferred to PVDF membranes ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10 to 30 μg of total protein was loaded onto NuPAGE Bis-Tris 4-12% acrylamide gels (Thermo Fischer Scientific) along with prestained protein ladder PageRuler (10-180 kDa) (Thermo Fisher Scientific) and run at 100 V ...
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bioRxiv - Cancer Biology 2021Quote: ... A total amount of 30 µg protein was loaded per lane of NuPAGE Tris-Acetate Protein Gel 3-8% (Life Technologies, EA0375BOX) and separated according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... and fractions were collected according to the SEC profile and SDS-PAGE (NuPAGE™ 4-12% Bis-Tris Protein Gels, Invitrogen™). Antibodies were stored at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... 20 μl of untreated and of ProK treated PMV (before dilution for FRET) was loaded to 8 % SDS-PAGE gels and the proteins were stained with GelCold Blue Stain (Thermo Fisher Scientific). A control in which PMSF was added for 15 min at room temperature before adding ProK also prepared and loaded on the gel.
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bioRxiv - Cell Biology 2021Quote: Equal amounts of protein were separated via SDS PAGE using a 1.5 cm 4-12% Bis-Tris gel (Thermo Fisher Scientific, NP0336) or 1 cm 4-12% Bis-Tris gel for ProQ diamond stain with 1x MOPS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The protein extracts are then centrifuged at 15000 rpm and separated on a NuPAGE 4%-12% Bis-Tris gel (Life Technologies, NP0321BOX). The non-specific sites are then blocked after the incubation for one hour with PBS Tween 0.1% (Tris Base NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... samples of each elution were loaded onto a poly-acrylamide gel which was stained by coomassie-blue followed by destain (Thermo Fisher Scientific). Eluates that contained the appropriate proteins were pooled together in a pre-chilled tube on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... we eluted the bound proteins with 2x SDS Laemmli buffer and ran total lysate on 4-12% bis-Tris gradient gel (NuPAGE, Thermo Fisher Scientific) for downstream Western blotting.