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Citations for Thermo Fisher :
8701 - 8750 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Slides were washed three times with PBS and incubated with goat anti-mouse IgG (H+L) Alexa Fluor® 647 (ThermoFisher #A21235) and goat antimouse IgG (H+L ...
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bioRxiv - Microbiology 2019Quote: ... were radioactively labeled on its 5’-end with 3 µL of γ-32P-ATP (3,000 Ci/mmol, Hartmann Analytic) per 20 μL reaction by using T4 polynucleotide kinase (Thermo Fisher Scientific), and further purified with the QIAquick Nucleotide Removal Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2019Quote: ... containing 4.5 g/L glucose, supplemented with 10% fetal bovine serum (FBS, Silantes, Munich, Germany) and 1% penicillin–streptomycin–neomycin (Invitrogen, Carlsbad, CA) at 37 °C in a humidified atmosphere of 5% CO2.
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bioRxiv - Neuroscience 2019Quote: ... 5% CO2 and 37°C in neuronal culture medium (Gibco Neurobasal medium supplemented with 1x Gibco B-27 supplement, 2 mM L-glutamine and 250 μM penicillin-streptomycin; all Thermo Fisher Scientific). After 24 h ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... targeting Vero cells line (ATCC® CCL-81™) cultured in complete medium containing 13.5 g/L EMEM (Gibco, Waltham, MA USA), 10% foetal bovine serum (Gibco ...
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bioRxiv - Genetics 2019Quote: ... 7.5 µl qPCR master mix and 266.7 nmol l−1 of each primer in a total volume of 15 µl (ThermoFisher Scientific, Wilmington, DE), as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... LOX activity in 15 μl of concentrated conditioned media was determined using the Amplex Red H2O2 detection kit following the manufacture’s instructions (Life Technologies, Carlsbad, CA). This assay is based on the ability of endogenous LOX to oxidize 10 mM DAP (a LOX substrate ...
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bioRxiv - Genomics 2020Quote: ... 2000) and derivatives were cultured in 4.5g/l glucose DMEM with 10% FBS and 2× NEAA (all Thermo-Fisher Scientific, Loughborough, UK). SK-MEL-28 cells (kind gift of Dr ...
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bioRxiv - Neuroscience 2020Quote: ... Polysomes were immunoprecipitated using monoclonal anti-EGFP antibodies (clones 19C8 and 19F7; see Heiman et al., 200830) bound to biotinylated-Protein L (Pierce; Thermo Fisher Scientific)-coated streptavidin-conjugated magnetic beads (Life Technologies) ...
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bioRxiv - Genomics 2020Quote: ... Cells were grown in HyClone RPMI-1640 medium (15% FBS [HyClone], 1% L-Glutamine and 1% Penicillin:streptomycin [Invitrogen]; 37°C, 5% CO2). RNA was extracted with Trizol LS (Invitrogen ...
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bioRxiv - Genetics 2020Quote: ... a cell suspension at each time point was spread on a poly-L-lysine-coated slide before observing in an EVOS FL Auto 2 inverted microscope (Thermo Fisher Scientific) with a 60X/1.42 PlanApo N Lens ...
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bioRxiv - Evolutionary Biology 2020Quote: MCF-7 cell line was procured from National Centre for Cell Sciences, (Pune, India) and cultured in GlutaMax-DMEM high glucose (4.5gm/l) (Gibco, Cat. No. 10569044) supplemented with 10% heat inactivated FBS (Gibco ...
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bioRxiv - Biophysics 2019Quote: Cells were cultured at 37°C in 5% CO2 in DMEM which contains 4.5 g/l of glucose and supplied by fetal bovine serum (Gibco/Life Technologies #16140-071) and 1% Pen Strep (Gibco/Life Technologies#15140-122 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then left untreated or subjected to heat-shock for 15 min prior to the addition of the methionine analogue L-azidohomoalane (AHA, 50 μM) (Thermo Fisher Scientific) and incubations continued for an additional 30 min.
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bioRxiv - Genomics 2021Quote: ... were transfected into 4×105 suspended U2OS pSLQ4356 L-A5 pSLQ6901 cells using the standard protocol for the Neon Transfection System 10 μL kit (Thermo Fisher, MPK1025). The transfected cells were plated in 96-well ibidi μ-plates and treated with 100 μM ABA or DMSO while seeding for 6 h ...
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bioRxiv - Genomics 2021Quote: ... The secondary antibody used in this study is Alexa Fluor® 647 Donkey Anti-Rabbit IgG (H+L) Antibody (Life Technologies A31573) used at 1:500 dilution.
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bioRxiv - Immunology 2021Quote: ... 2 μL MgSO4 (2 mM final concentration) and 0.2 μL Invitrogen™ Platinum™ Taq DNA Polymerase High Fidelity(1IU; all ThermoFisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... The secondary antibodies used in a study were as follows: donkey anti-rabbit IgG (H+L, Highly Cross-Adsorbed, Alexa Fluor 488; 1:500, catalog: A-21206, Thermo Fisher Scientific) and donkey anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... cells (ATCC CCL-10) were maintained in DMEM containing 10% fetal bovine serum (FBS; Cell Sera F31803) and 5 mM L-Glutamine (Invitrogen, 25030-081) at 37°C under 5% CO2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 100 µL of this culture was pipetted into each well of a sterile 96-well plate (Nunc™Edge 2.0, Thermo Scientific™) with the edge moats filled with 1.7 mL of sterile H2O ...
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bioRxiv - Biophysics 2021Quote: ... Cover glasses were mounted on the imaging chamber and DMEM medium was replaced by pre-warmed L-15 imaging medium (cat. no. 11415049, Gibco, Fisher scientific). Imaging was done at 37°C on a 3i second generation lattice-light-sheet microscope with a 0.71 NA LWD WI objective for excitation and a 1.1 NA WI objective for imaging and equipped with 2 Hamamatsu ORCA-Flash 4.0v3 sCMOS cameras for simultaneous dual color imaging providing a 62.5x magnification with 230×230×370 nm (x-y-z ...
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bioRxiv - Biochemistry 2020Quote: ... Peptide sample (2 μL) was injected onto an Acclaim PepMap 1000 trap column (75 μm × 2 cm, nanoViper C18 3 μm, 100Å, Thermo Fisher Scientific) which was heated to 40 °C in a chamber which was connected to a C18 reverse-phase Aurora UHPLC Emitter Column with nano Zero & Captive Spray Insert (75 μm × 25 cm ...
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bioRxiv - Bioengineering 2021Quote: ... followed by incubation for 2 hours in Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody Alexa Fluor 647 (Thermofisher, A-21235) or Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2020Quote: ... unreacted PTAD was removed by dialysis overnight in 1 L of 500 mM Tris (pH 7.4) with Slide-A-Lyzer™ G2 dialysis cassettes (10 K MWCO, Fisher Scientific, #87729). CD spectra were averaged from two or three replicates.
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bioRxiv - Biochemistry 2020Quote: ... at 37°C in Dulbecco’s modified Eagle’s medium (DMEM) with 4.5 g/L glucose and GlutaMAX™ (Product No. 35050061, ThermoFisher Scientific, Carlsbad, CA, USA) or 20% heat-inactivated fetal calf serum (FCS) ...
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bioRxiv - Cell Biology 2021Quote: ... Akata cells were grown at 37°C under 5% CO2 in RPMI-1640 supplemented with 10% FCS and 2 mM of L-Glutamine (Gibco, 25030-081). Cells KD for BHRF1 were generated from Akata cells (see below ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... 8 µL of PFFs were diluted with 32 µL HBSS (HBSS minus calcium and magnesium, no phenol red, 14175-053, Thermo Fisher Scientific) and mixed briefly 5 times ...
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bioRxiv - Physiology 2020Quote: ... containing 1.5 ml of chilled (4°C) transfer media composed of Ham’s F-10 medium including 1 mM L-glutamine (LG; Thermo Fisher Scientific, Denmark), 0.1% heat inactivated fetal bovine serum (hiFBS ...
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bioRxiv - Physiology 2020Quote: ... and 32 μl of RNA with an average yield of 48 ng/μl were reverse transcribed with Superscript II reverse transcriptase (Life Technologies, Germany) to obtain 80 μl of cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... glucose, vitamin B12, folic acid, phenol red (US Biological, R9001-02) were further supplemented with 300 mg/l glutamine (Thermo Fisher, 25030081), 25 mM glucose ...
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bioRxiv - Cell Biology 2021Quote: ... cultured parasites were washed twice in SDM79 medium without serum and spread directly onto poly-L-lysine coated slides (Fisher Scientific J2800AMMZ). Cells were left for 10 minutes to settle prior to treatment with 1 volume 4% PFA solution in PBS at pH 7 ...
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bioRxiv - Microbiology 2021Quote: ... immunostained with rabbit anti-O78 antibody followed by cross absorbed goat anti-rabbit AlexaFluor 594-conjugated IgG (H+L) (ThermoFisher A-11072). Membranes with fluorescently labeled samples were excised and mounted onto glass slides with ProLong Gold (ThermoFisher Scientific P36930) ...
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bioRxiv - Neuroscience 2020Quote: ... The day before transfection, 1× 106 HEK293T cells were seeded in 10cm plate (DMEM medium supplemented with FBS and L-Glutamine, (Gibco, Ottawa, ON)) ...
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bioRxiv - Genomics 2021Quote: Cryopreserved stocks of the KH004 parent and the Mal31×KH004 uncloned bulk recombinant progeny pool and were thawed and grown in complete media (CM) [RPMI 1640 with L-glutamine (Gibco, Life Technologies), 50 mg/L hypoxanthine (Calbiochem ...
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Pax6 loss alters the morphological and electrophysiological development of mouse prethalamic neuronsbioRxiv - Developmental Biology 2021Quote: ... 130μl of the culture medium containing the desired number of resuspended cells was then added onto the 9mm circular coverslips (Fischer Scientific) coated with Poly-L-lysine and Laminin (Thermo Fisher Scientific). Due to the surface tension of the culture medium ...
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bioRxiv - Neuroscience 2020Quote: ... the blots were incubated for 1 hr with goat anti-rabbit HRP conjugated IgG (H+L) (1:1000; Thermo Scientific, Waltham, MA). PierceTM ECL Western blotting substrate (Thermo Scientific ...
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bioRxiv - Physiology 2021Quote: ... Membrane damage was assessed using anti-IgG staining using an anti-rat antibody (IgG H+L AlexaFluor® 647 1:100, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and 20 μl of such mixtures were loaded on 96-well white plates (Microamp Optical 96-well Reaction Plate with Barcode, Applied Biosystems, #4306737), containing 5 μl of an appropriate 5x buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Secondary antibodies for immunofluorescence were Alexa Fluor™ 488 Goat anti-Rabbit IgG (H+L) (Cat no. A11034, 1:500, ThermoFisher Scientific), Alexa Fluor™ 546 Goat anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... cells were cultured in RPMI 1640 plus L-glutamine and 25 mM HEPES media (Gibco RPMI 1640 Medium, HEPES, Thermo Fisher Scientific), supplemented with 10% heat-inactivated fetal bovine serum (hiFBS ...
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bioRxiv - Cell Biology 2021Quote: ... were maintained in DMEM with 1.5 g/L sodium bicarbonate & sodium pyruvate (Wisent Bio Products 319-007-CL) supplemented with 10% heat-inactivated fetal bovine serum (Gibco 12483-020).
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with respectively Alexa Fluor 555-conjugated goat anti-mouse IgG (H+L) (Thermo Fisher Scientific #A21422, diluted 1:400) and 488-conjugated goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... Stefan Finke) and secondary antibody goat anti-rabbit IgG (H+L) conjugated with Alexa Fluor 488 (dilution 1:1000; Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2020Quote: ... Cells are plated at constant density (250000 cells in each M24 well) for all experiments in Neurobasal medium without L-glutamine (ThermoFisher 21103-049), Glutamax (ThermoFisher 35050-038) ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed 3 times for 10 min in PBS 1X + 0.1% Triton X-100 + 1% BSA and incubated for 6h with secondary antibodies at 4°C: goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 568 (1/500, Invitrogen #A-11031), goat anti-rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2022Quote: Dry peptides were reconstituted in 5 μl water and labeled by adding 2 μl TMT label (20 μg/μl in acetonitrile (ACN)) (TMTpro 16 plex, Thermo Fisher Scientific) and incubating 1 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-Goat Mouse IgG (H+L) cross-adsorbed secondary antibody with Alexa Fluor 647 (Thermo Fisher Scientific, catalog number A-21235). The colocalized areas from YFP-Parkin and anti-Tom20 fluorescence were quantitated using Nikon NIS-Elements AR imaging software.
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bioRxiv - Bioengineering 2022Quote: ... they were washed twice with DPBS and incubated with goat anti-rabbit IgG (H + L)-Alexa Fluor 647 (1:1000, Invitrogen, Waltham, MA) for 1 h at room temperature ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... and were seeded in 100-mm round tissue-culture treated polystyrene dishes at 1 million cells/mL in complete RPMI medium (with L-glutamine, Gibco, Cat# 11875119) with 10% characterized fetal bovine serum (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... slides were incubated with a purified anti-CD11b/c (a list of the clones and suppliers of all mAbs used in the study in Supplemental Table 1) and the staining was revealed using a goat anti-mouse IgG (H+L)-AF488 (Invitrogen, Carlsbad, CA). After staining ...