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Citations for Thermo Fisher :
8651 - 8700 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: Purified Sl-Enc1/His-Sl-Enc2 protein samples were analyzed via SDS-PAGE with a 14% Novex Tris-Glycine Gels (Thermo-Fisher Scientific). The SDS-PAGE gel was stained with Coomassie and the overexpressed bands at the correct molecular weights were excised and stored in water ...
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bioRxiv - Biochemistry 2024Quote: ... plasmid isolation and PCR product extraction from agarose gels were carried out using GeneJET kits (Thermo Fisher Scientific, USA, #K0503, #K0832). Site directed mutagenesis was carried out using Phusion™ Plus DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... Native PAGE or SDS PAGE gels were transferred to a nitrocellulose membrane using an iBlot2 (20 V, 7 min; Invitrogen™). The membrane was incubated with blocking buffer (PBS ...
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bioRxiv - Biophysics 2024Quote: ... protein fractions were mixed 1: 1 with SDS loading buffer and loaded on a NuPageTM 4-12% Bis-Tris PAGE Gel (Invitrogen™) along with Novex™ Sharp Pre-stained Protein Standards (range from 3.5 to 260 kDa ...
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bioRxiv - Biochemistry 2024Quote: ... Protein isolates were mixed with protein loading buffer containing 100 mM DTT reducing agent and denatured for 5 min at 95°C before loading onto 4–12% Bis-Tris NuPAGE precast gels (Thermo Fisher) for electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured by boiling for 15 minutes and separated on a 15-well Novex WedgeWell 4-20% Tris-Glycine Gel (Thermo Scientific) before transfer to a 0.22µm PVDF membrane overnight at 4°C ...
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bioRxiv - Genomics 2024Quote: ... After the de-staining in-gel trypsin digestion was performed and samples were loaded into Mass spectrometry using Q Exactive (Thermo Scientific) interfaced with nanoflow LC system (Easy nLC 1200 ...
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bioRxiv - Biochemistry 2024Quote: ... 20 μg of total protein (from whole cell extracts or isolated lysosomes) was separated with a 4%–12% Bis-Tris Plus Gels (Invitrogen, Thermo Scientific) and then transferred onto PVDF (polyvinylidene difluoride ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were incubated for 10 min at 95 °C and were immediately loaded in 4%–12% Bis-Tris Plus Gels (Invitrogen, Thermo Scientific) and ran at constant 120 V (see below).
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bioRxiv - Pathology 2024Quote: Western blotting was performed using standard procedures as described previously.26 A total of 15 µg of protein was fractionated using NuPAGE 4–12% Bis-Tris Mini gels (Thermo Scientific) and transferred to Protran nitrocellulose transfer membranes (Whatman) ...
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bioRxiv - Molecular Biology 2024Quote: ... Either 20 μL of the FLAG elution described above or 1.1 pmol purified Tom1p were loaded to a NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Invitrogen) and electrophoresed in MOPS buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The protein lysates were run on 12.5% SDS-PAGE gel and were transferred onto 0.2 µm PVDF membrane (Thermo Scientific, cat# 88520). The membrane was incubated in blocking buffer consisting of 5% non-fat dried milk in 1x Tris-buffered saline/0.1% Tween 20 (TBS-T ...
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bioRxiv - Microbiology 2024Quote: ... 0.25% Orange G w/v in water) before loading on to a 0.7% agarose gel in TAE buffer containing SybrSafe dye (ThermoFisher Scientific, S33102). DNA bands were visualized over blue light and a clean razor was used to cut out gel portions containing bands greater than 2 kb in length ...
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bioRxiv - Microbiology 2024Quote: ... for standard protein analysis or to native PAGE using a NativePAGETM 3‒12% mini protein gel and a Sample Prep Kit (Thermo Fisher) for analysis of the heptamerization of PA28γ ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified DNAs from excised gel slices were cloned into a pCR4Blunt-TOPO vector using a Zero Blunt TOPO PCR Cloning Kit for Sequencing (ThermoFisher Scientific) and subjected to Sanger DNA sequencing using M13 forward and M13 reverse primers (Eurofins Genomics).
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bioRxiv - Cell Biology 2024Quote: Samples from Co-IP experiments were resolved by SDS-PAGE in a gradient polyacrylamide gel Bolt™ 4-20% Bis-Tris Plus (Invitrogen). After running ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates were loaded into a 10-well 10% Bis-Tris SDS-polyacrylamide gel along with PageRuler Plus Protein ladder (26619, ThermoFisher Scientific). Proteins were separated by electrophoresis at 130V for 90 min and subsequently transferred to 0.2 micron pore size nitrocellulose membrane.
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bioRxiv - Molecular Biology 2024Quote: ... The quality of extracted RNA was identified by visualization of the RNA integrity using 1% agarose gel and using a spectrophotometer (NanoDrop; Thermo Scientific). The final RNA concentration was adjusted to be equal in all the samples and cDNA was synthesized for each sample using a TAKARA cDNA synthesis kit for real-time gene quantification ...
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bioRxiv - Plant Biology 2024Quote: ... Amplicons were visually inspected by gel electrophoresis and prepared for sequencing by treatment with alkaline phosphatase FastAP and exonuclease ExoI (Thermo Scientific) for 15 min at 37°C followed by 20 min at 85°C ...
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bioRxiv - Plant Biology 2020Quote: ... The integrity and approximate concentration of the extracted RNA was determined by electrophoresis of the RNA extracts in a 1% agarose gel containing Sybr Safe (Invitrogen, Carlsbad, CA). One microgram of total RNA was reverse transcribed to complementary DNA (cDNA ...
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bioRxiv - Plant Biology 2020Quote: ... Cleared cell lysate was denatured with SDS buffer and separated by SDS–PAGE with Novex 4–12% Tris-glycine polyacrylamide gel (Invitrogen, Life Technologies, CA). Western blot analysis was performed using anti-mCherry antibody (16D7 ...
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bioRxiv - Biophysics 2021Quote: ... RNA concentration was determined by 260 nm absorbance and verified for purity and size using a denaturing agarose gel and Millenium RNA ladder (ThermoFisher Scientific AM7151).
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bioRxiv - Biochemistry 2020Quote: Equal amounts of protein (20-50 μg) were loaded and were separated by electrophoresis in 4 to 12% Bis-Tris Gel (Thermo Fisher Scientific), transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Biophysics 2020Quote: Cut gel bands were de-stained with a wash buffer (50% aqueous acetonitrile with 50 mM ammonium bicarbonate) and cut into ∼ 1 mm gel pieces and dried by incubation with acetonitrile followed by SpeedVac (Thermo Fisher Scientific). Digestion buffer (10% aqueous acetonitrile with 50 mM ammonium bicarbonate ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples were run on 10% Bolt Bis-Tris Plus gels with NuPAGE MES SDS running buffer (Thermo Fisher Scientific, Waltham, MA). Transfer to PVDF membranes in NuPAGE transfer buffer was carried out at 10 V overnight at 4°C ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... A total of 30 μg of protein per sample was separated on NuPAGE 4-12% Bis-Tris pre-cast polyacrylamide gels (Novex, Thermo Fisher Scientific), transferred onto iBLOT2 Polyvinylidene Difluoride (PVDF ...
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bioRxiv - Immunology 2021Quote: ... proteins from cell lysates were separated by 10% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using MOPS running buffer (Thermo Fisher, USA), and transferred to a nitrocellulose membrane (Millipore ...
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bioRxiv - Genetics 2021Quote: ... The gDNA sample integrity was controlled on 1% agarose gel and quantified on Nanodrop and Qubit using the Qubit™ dsDNA BR Assay Kit (Invitrogen™). 2 × 2 µg of gDNA were digested in parallel with 50 units of DpnII (NEB #R0543L ...
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bioRxiv - Genetics 2021Quote: ... Samples were heated at 100 °C for 10 min prior to electrophoresis through a Novex NuPAGE 4–12 % Bis-Tris gel (Life Technologies, # NP0335BOX) in MES SDS running buffer (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Samples were boiled in 95°C for 10 minutes and loaded into NuPage Novex 4-12% Bis-Tris SDS-PAGE Gels (Thermo Fisher Scientific), electroblotting was performed using the Biorad Trans-Blot Turbo Transfer System ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products detected on 6% polyacrylamide or 2% agarose gels were purified with a Charge Switch PCR Clean-Up kit (Invitrogen, Carlsbad, CA) and then sent to BMR genomics (Padova ...
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bioRxiv - Evolutionary Biology 2020Quote: ... an enhanced chemiluminescence substrate was used to visualise the blotting results in an iBright CL1000 gel imaging system (Thermo Fisher Scientific, USA). The densitometry analyses of the bands were performed using ImageJ (62).
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bioRxiv - Evolutionary Biology 2021Quote: ... We then visualized PCR products on agarose gels and quantified them with the Qubit Broad Range kit (Thermo Fisher, Waltham, MA, USA) and normalized samples to 540ng of cDNA ...
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bioRxiv - Neuroscience 2021Quote: ... 20 µl of either type of samples were run on BOLT 4-12% Bis-Tris mini protein gels for SDS-PAGE and electroblotted using the iBlot 2 dry blotting system (Thermo Fisher, USA). Polyvinylidene fluoride membranes were probed with anti-PrP monoclonal antibody ICSM-18 (1:4,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... in vitro transcription products were purified with a GeneMATRIX Universal RNA purification kit (EURx) and visualized on a 10% PAA gel with SYBR Safe reagent (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... the amplified products were visualized in 2% agarose gel electrophoresis in 1X TAE buffer and stained with SYBR Safe (Invitrogen, Waltham, MA) according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were incubated at 90C for 10min and then loaded on a pre-run (75v, 10min) NuPAGE Bis-Tris Gel (Life Technologies NP0307) with 1x MOPS running buffer and run for 10-15min at 75v and then 120V until each sample has been satisfactorily separated ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.2 M DTT] and briefly boiled at 95°C for 5 min before loading 1% on a 10% Tris-Glycine SDS-PAGE gel (Thermo Fisher Scientific) and run for app ...
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bioRxiv - Molecular Biology 2021Quote: LC-MS/MS analyses for samples that were digested on beads were performed on a Q Exactive mass spectrometer and for samples processed by in-gel digestion on an Orbitrap Fusion Lumos Tribrid mass spectrometer (both Thermo Fisher Scientific), both coupled on-line to Ultimate 3000 RSLCnano Systems (Dionex ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified RNA amounts were quantified using nanodrop and verified for purity and size using a denaturing agarose gel and Riboruler RNA ladder (Thermo Scientific SM183).
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bioRxiv - Molecular Biology 2021Quote: ... subjected to in-gel digestion with trypsin and submitted for LC-MS/MS analysis (LTQ Orbitrap Velos Pro mass spectrometer; Thermo Fisher Scientific). Three biological replicates were performed for each genotype.
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bioRxiv - Molecular Biology 2021Quote: ... Solubilised proteins in a 25 μL volume were prepared and separated by electrophoresis on NativePAGE 3-12% Bis-Tris gels (Thermo Fisher Scientific) and NuPAGE Tris-Acetate buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... and 5 μL of input and 10 μL of eluate were separated on NuPAGE 3-8 % Tris-Acetate gels (Thermo Fisher Scientific) run at 4 °C and 35 mA/gel for 1.5 h ...
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bioRxiv - Biophysics 2022Quote: ... Approximately 50 μg proteins were loaded onto 15% (v/v) denaturing SDS-PAGE gels and stained using Coomassie Brilliant Blue G-250 (ThermoFisher Scientific, UK). Immunoblot analyses were performed using anti-RbcL (1:10,000 diution ...
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bioRxiv - Cell Biology 2022Quote: ... fluorescent transgenic or mRNA injected embryos (injection protocols in [33]) were mounted in 2% low melting agarose gel (Thermo Fisher Scientific; 16520100) at 512-cell stage and imaged using SP8 LSCM or spinning disk confocal microscopes.
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bioRxiv - Genomics 2020Quote: ... QC was performed using absorbance assay for quantity and running a sample on a 4% agarose E-Gel (#G800804, Thermo Fisher Scientific) to check for fragmentation ...
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bioRxiv - Genomics 2020Quote: ... RT-PCR products were fractioned by electrophoresis using 6% polyacrylamide gels in 1 x TBE and Sybr safe staining (Thermo scientific, S33102). The bands corresponding to exon inclusion or skipping were quantified using ImageJ 1.47v (NIH ...
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bioRxiv - Genomics 2019Quote: ... boiled in 2X NuPAGE LDS Sample Buffer (including 1.8% β-Mercaptoethanol) for 5min and separated in NuPAGE 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific).
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bioRxiv - Genomics 2019Quote: ... boiled in 2X NuPAGE LDS Sample Buffer (including 1.8% β-Mercaptoethanol) for 5 min and separated in NuPAGE 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific).
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bioRxiv - Genomics 2019Quote: ... Ground tissue was boiled in 2X NuPAGE LDS Sample Buffer (including 1.8% β-Mercaptoethanol) for 5min and separated in NuPAGE 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific). Following antibodies were used for immunoblotting ...