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Citations for Thermo Fisher :
8651 - 8700 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 100 µl of diluted phage and 300 µl of bacterial culture at OD600 between 0.4 and 0.5 as measured on a Genesys 30 Spectrophotometer (Thermo Scientific, Waltham, USA) were added to 3 ml of molten LM17-CaCl 0.75% w·v-1 agar and poured over LM17 1% w ·v-1 agar plates ...
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bioRxiv - Neuroscience 2024Quote: ... and the secondary antibody used was a goat anti-mouse IgG (H+L) with either an Alexa Fluor 488 (1:250; Invitrogen A11029) or Alexa Fluor 647 congugate (1:250 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed three times with 1X PBT for 5 minutes each and then incubated for 1 h at room temperature with secondary antibody (goat anti-rabbit IgG (H+L) Alexa Flour 546 (Invitrogen, A11035) or goat anti-mouse IgG (H + L ...
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bioRxiv - Microbiology 2024Quote: ... between 5 and 50 μL of cell sample was dropped onto either poly-l-lysine coated chambered slides (Nunc Lab-Tek 8-Well chambered slides, Thermo scientific) or 10-well diagnostic slides (MP Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was adjusted to pH 5.8 using 1N KOH prior to addition of 6 g/L Phytagar (Gibco, Life Technologies, U.S.A) and autoclaving for 20 min at 121°C ...
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bioRxiv - Genomics 2024Quote: ... DNA samples were eluted in 30 µl 10 mM Tris HCl pH 8 and analysed for purity and quantity using the Nanodrop spectrophotometer (NanoDrop One, ThermoFisher Scientific) and Qubit fluorometer (Invitrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 0.2 μl 50x oligos of the AarI recognition site for Level 0 and Level 2 cloning or Eco31I/BsaI-HFv2 (ThermoFisher/NEB) for Level 1 cloning ...
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bioRxiv - Plant Biology 2020Quote: ... 10 μL of peptide digests were loaded onto a C18 PepMap trap column (300 μm inner diameter x 5 mm, Thermo Fisher Scientific) at a flow rate of 30 μL/min ...
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bioRxiv - Plant Biology 2020Quote: ... Enzyme-treated and mock-treated samples were diluted down to 10 ng µl-1 and 1 µl DNA was amplified by qPCR using 200 nM of each primer and Power Up SYBR Green Master Mix (Thermo Fisher Scientific). Details on the primers used for the analysis can be found in Table S2 ...
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bioRxiv - Biophysics 2020Quote: ... A single colony from the LB-agar plate with ampicillin was inoculated into 10 mL of media containing 20 g/L tryptone (Fisher Scientific, BP1421), 10 g/L yeast extract (Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed three times with PBS on the next day and incubated with corresponding secondary antibodies goat anti-rabbit IgG (H+L) FITC (Thermo Fisher Scientific), goat anti-mouse IgM (H ...
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bioRxiv - Cancer Biology 2021Quote: ... The wells were washed four times with PBST and were incubated at RT with Goat anti-rabbit IgG (H+L) Cross-adsorbed -HRP conjugate secondary Ab (ThermoFisher G-21234) for 1h ...
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bioRxiv - Microbiology 2020Quote: ... isolates were revived from glycerol stocks into 500µL liquid LB-L media in wells of 2.0mL 96-well DeepWell™ blocks (ThermoFisher™ Nunc™). The blocks were incubated with shaking until the cultures reached stationary phase ...
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bioRxiv - Molecular Biology 2019Quote: ... purified RNA was resuspended in 100 μl RNase-free water and DNase treated using the TURBO DNA-free kit according to the manufacturer’s instructions (Invitrogen - catalogue no. AM1907M). Residual inactivation beads were removed by spinning the RNA sample through a QIAshredder column at 1000 g for 1 min (Qiagen-catalogue no ...
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bioRxiv - Cancer Biology 2021Quote: ... purchased from Jackson Immunology) and Alexa Fluor® 555 AffiniPure Donkey Anti-Rabbit IgG (H+L) (1:200, purchased from Thermo Fisher) for 120 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed and incubated with Alexa Fluor 488 goat anti-rabbit (H+L) antibody (1:1,000; Thermo Fisher Scientific; A-11008) for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% FBS and 1% penicillin-streptomycin) and eWAT cells were cultured in ITS media (ITS media: 56.65% 1g/L glucose DMEM (Gibco, Cat#11885-084), 37.65% 1X MCDB210 pH 7.25 (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... transfection medium was removed and replaced with 150 μl/well of DMEM with 4.5 mg/ml glucose and sodium pyruvate without L-glutamine and phenol red (w-G-SP, wo-G-PR) (Thermo Fisher Scientific) containing 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... Antibody was harvested from the cell supernatant after 5 to 7 days post inoculation and purified using Protein L Sepharose beads (Thermo Fisher Scientific).
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bioRxiv - Genetics 2021Quote: ... The sections were rinsed in PBS/Tween buffer before incubating for 60 min in 1:200 goat anti-rat IgG (H+L) antibody conjugated to horseradish peroxide (HRP) (Invitrogen, ThermoFisher Scientific). The slides were then developed in 3,3’ diaminobenzidine (DAB ...
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bioRxiv - Microbiology 2019Quote: ... using a Micro Amp Fast Optical 48-well reaction plate with 15 μ L reaction volume containing 1 x Power Sybr Green PCR Master Mix (Thermo Fisher), 0.2 μM of each primer and 1.5 μg of cDNA ...
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bioRxiv - Developmental Biology 2021Quote: ... The spermatozoa were washed three times with PBS and incubated with x1/2000 Alexa 488-conjugated anti-mouse IgG (H+L) (Thermo Fisher Scientific) or x1/2000 DyLight550-conjugated anti-rabbit IgG (Abcam ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Fluorescently labeled samples were mounted in glycerol mounting solution (80% glycerol, .1M Tris, pH 8.0) on microscope slides coated with poly-L-lysine (Thermo Fisher Scientific #86010). Samples for all species except D ...
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bioRxiv - Evolutionary Biology 2021Quote: ... interaction zones containing protozoan cells were gently harvested using a 1 μl loop and resuspended in 10 μl of Tris HCl buffer and stained with 2% Propidium Iodide (V/V) (Thermo Scientific, P3566). PI stains cells that have lost membrane potential ...
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bioRxiv - Developmental Biology 2020Quote: ... 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (#A32733, 1:1000 dilution)(all Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... All cell lines were cultured under standard conditions in ES medium (DMEM/Gibco, 15 % FBS/Gibco, 2mM L-glutamine/Thermo Fisher Scientific, 1X Penicillin Streptomycin/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 200,000 cells were transfected in 12 well plate with 25 pmol siRNA (siTFEB : ON-TARGETplus Human TFEB, L-009798-00-0005, Dharmacon™) using Lipofectamine RNAiMAX Transfection Reagent (1:200; Life Technologies). Cells were incubated 72 h at 37°C prior to further manipulation or drug treatment ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then washed with PBS and incubated with Alexa Fluor 647 Donkey Anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody (Invitrogen A-21447) in PBS at 4°C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... diced tissue was incubated at 37°C with gentle rocking in DMEM/F-12 (with L-glutamine and HEPES, ThermoFisher Scientific 11330032) supplemented with collagenase (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... while for the ECAR measurements the media was supplemented with 1 mM of Sodium Pyruvate and 2 mM of L-glutamine (both from Life Technologies, UK), pH 7.4 at 37°C.
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bioRxiv - Neuroscience 2021Quote: ... Sodium pyruvate (2 mM), MEM amino acids solution (50X, without L-Glutamine) and MEM vitamins solution (100X) were added from concentrates (Fisher Scientific, UK). The pH was adjusted to 7.5 (osmolality ~308 mmol kg-1).
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bioRxiv - Molecular Biology 2021Quote: ... Slides were then incubated with Alexa 594 goat-anti rabbit secondary antibody at 1:750 and with Alexa Fluor 488 goat anti-human IgG(H+L) at 1:500 (both Invitrogen; in PBS) for 2 h at RT.
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bioRxiv - Molecular Biology 2021Quote: ... One µl of each DNA sample was used as template for qPCR with Applied Biosystems PowerUp™ SYBR™ Green Master Mix (ThermoFisher) and primers targeting the X ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µl of each cDNA sample was used as template for qPCR using Applied Biosystems PowerUp SYBR Green Master Mix (Thermo Fisher Scientific). X ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for one hour on the rocker at room temperature in blocking solution with 1:1000 Goat anti-Rabbit IgG H&L Alexa Fluor 405 (Thermo Fisher Scientific). Then sections were washed once in PBS-T ...
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bioRxiv - Molecular Biology 2021Quote: ... Add 100 µL Pol II antibody-conjugated beads (10 µg Pol II 8WG16 antibody conjugated with Dynabeads™ Protein G for Immunoprecipitation (ThermoFisher, #1004D)) and incubate on a tube rotator for 1 hour in the 4 °C room ...
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bioRxiv - Molecular Biology 2020Quote: ... MSC and HEK cell-lines were maintained in high-glucose (4.5 g/l) DMEM GlutaMax (Thermo Fisher Scientific, Waltham, MA, USA; HEK) or RPMI medium 1640 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... which was replaced the next day with human neural induction media (DMEM HAM’s F12, with 50 μg/ml penicillin-streptomycin, 2 mM L-glutamine, 1:100 of N2 (Invitrogen, cat. 17502-048), 1:50 of B27 (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 2µl lysis buffer with 1U/µl Rnase Inhibitor (RRI) and ERCC spike in mix at a 1:20 dilution (Ambion Cat #4456740). Plates were promptly sealed (Thermo Fisher Scientific #AB0626) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were cultured in complete RPMI media (RPMI 1640 plus L-glutamine and 25 mM HEPES media (Gibco RPMI 1640 Medium, HEPES, Thermo Fisher Scientific), supplemented with 10% heat-inactivated fetal bovine serum (hiFBS ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μL of cDNA was assayed in duplicate in a 20 μL reaction composed of TaqMan Universal PCR Master Mix (Thermo Fisher Scientifc), 450 nM primers ...
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bioRxiv - Immunology 2022Quote: ... 10% foetal calf serum, 10 units/ml penicillin, 10 μg/ml streptomycin and 2 mM L-glutamine, all supplied by Gibco, ThermoFisher UK). All cells were incubated for 72 hours with Myelin Oligodendrocyte Protein (MOG ...
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bioRxiv - Immunology 2022Quote: ... 10% foetal calf serum, 10 units/ml penicillin, 10 μg/ml streptomycin and 2 mM L-glutamine, all supplied by Gibco, ThermoFisher UK). All cells were differentiated for 48 hours in the presence of plate-bound αCD3 (2.5ug/ml ...
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bioRxiv - Immunology 2021Quote: ... 50 μl serially diluted pMHC were added to each well of high-binding capacity streptavidin-coated 96-well plates (#15500; Thermo Fisher Scientific). After a minimum 45-min incubation at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... primary antisera incubated sections were washed three times in PBS each for 10 minutes and subsequently incubated for 2 hours at room temperature in PBS with 2% NGS plus a dilution of an Alexa Fluor 488 goat anti-rabbit IgG (H+L) (1:1000, Molecular Probes, #A11034) or Alexa Fluor 594 goat anti-mouse IgG (H+L ...
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bioRxiv - Molecular Biology 2019Quote: ... 3 μl of FuGENE® 6 and 1.3 μg of plasmid were added to 100 μl of OPTI-MEM Medium (Thermo Fisher Scientific). The mixture was incubated at room temperature for 30 minutes prior to the addition to the coverslips.
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bioRxiv - Genetics 2019Quote: ∼50μl pellets of L4 stage worms were flash frozen in liquid nitrogen and RNA extraction was performed using TRIzol (Life Technologies, USA) and chloroform (Sigma ...
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bioRxiv - Bioengineering 2019Quote: ... A 100 μl aliquot of Matrigel was spread uniformly over the surface of a Lak-Tek 4-well chambered coverglass well (ThermoFisher, Catalog #155383) chilled on ice and placed in an incubator at 37°C for 5 minutes to polymerize ...
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bioRxiv - Evolutionary Biology 2019Quote: ... were inoculated on the top of a test tube containing agar and tributyrin for lypolytic activity measure (10 mL/L, ACROS Organics, Belgium) or semi-skimmed milk for the proteolytic activity measure (40 g/L ...
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bioRxiv - Microbiology 2019Quote: ... which were generated by combining defined ratios of DMEM with or without L-glutamine (41965, 25 mM glucose, 4 mM glutamine; and, 11960, 25 mM glucose, no glutamine; Thermo Fisher Scientific).