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Citations for Thermo Fisher :
8601 - 8650 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... We obtained 40-80 μg of histone proteins per extraction and loaded 20 μg of protein onto a 4-12% Bis-Tris Plus mini gel (Invitrogen# NW04120BOX). Gels were run for ∼20 minutes at 200V using the Invitrogen mini gel tank with Blot MES SDS Running buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The samples were first heated for 5 min at 95°C and then centrifuged at 11,000xg in microfuge before loading them on 4-12% pre-cast mini-or midi-gels (Thermo-Fisher Scientific/Life Technologies). The running buffer contained MOPS [3-(N-morpholino ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 µL of the cell-gel solution was added into each well of a 16-well chamber slide (Thermo Fisher Scientific). Cells were thoroughly mixed in the pre-gel solution and then incubated at 37°C for 30 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Five μL of each PCR product was mixed with loading dye and run on a 1 % agarose gel (SYBR Safe; Life Technologies) at 90 V for ∼45 min ...
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bioRxiv - Genomics 2023Quote: ... Cell lysates with equal amounts of total protein (15-20 μg) were loaded and separated on a SDS polyacrylamide gel (10–15%) and were electrophoretically transferred onto nitrocellulose membranes (Thermo Scientific). The membranes were blocked with non-fat dry milk in TBST (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... After the PCR run 2 µl of the PCR reaction was run on a 1% agarose gel containing ethidium bromide and the GeneRuler 1 kb Plus DNA ladder (Thermo Scientific) was used as a DNA size reference ...
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bioRxiv - Microbiology 2023Quote: ... 10 µl of the whole cell lysate was loaded on 15% resolving gel and subjected to mouse primary anti-FLAG antibody (Thermo Scientific) and secondary goat anti-mouse HRP-conjugated antibody (Cell Signalling Technology).
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bioRxiv - Microbiology 2023Quote: ... The concentration of protein was then assessed via BCA and samples were normalized and added to sample buffer supplemented with 10% BME before being run on an SDS-PAGE gel (Invitrogen NW04125BOX). Gels were transferred using an iBLOT transfer system (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... Neurons were transfected with pEGFP-C1 vectors and cultured on polyacrylamide gels with embedded 200-nm fluorescent beads (Thermo Fisher Scientific) for 2 days ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μg total RNA was denatured in formamide loading dye and resolved on the 6% TBE-Urea gel (Novex, ThermoFisher Scientific) in 1 X TBE buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products were analyzed on a 1.8% agarose TBE-gel with GeneRuler 100 bp Plus DNA Ladder for sizing (Thermo Scientific, #SM0322). The intensity of the bands reflected the expression level of the splice isoforms in the original RNA ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µg total RNA was resolved on 1% agarose gel with TRI/TRI buffer and overnight capillary transfer onto BrightStar-Plus Positively Charged Nylon Membrane (ThermoFisher Scientific). The detailed method and hybridization conditions are published (Robertson et al ...
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bioRxiv - Cell Biology 2023Quote: ... 4 and 5 we confined cells by using a soft acrylamide gel and either 1% Low Melting Agarose (Invitrogen, 16520-100) or a second soft acrylamide gel ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were tested at a final concentration of 7.5 µM in a 50 mM Tris (pH 8.0) and supplemented with 1x SYPRO™ Orange Protein Gel Stain (Life Technologies) after incubation with the compounds for 30 minutes on ice ...
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bioRxiv - Cell Biology 2023Quote: ... The reactions were quenched with final concentration 100 mM Tris–HCl before separation on Bolt™ 4–12% Bis-Tris Plus gels (Invitrogen). Sulfo-SDA (sulfosuccinimidyl 4,4’-azipentanoate ...
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bioRxiv - Cancer Biology 2023Quote: ... and subjected to electrophoresis in 10% or 4–12% Bis-Tris gels and transferred to nitrocellulose membranes using iBlot2 (Life technologies). Membranes were probed for VEGF-C followed by horseradish peroxidase-conjugated secondary antibodies (Thermo Fischer Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were then separated on a NuPAGE NOVEX 4-12% gradient Bis– TRIS pre-cast gel (Thermo Fisher Scientific, Waltham, MA) at 150V for 1 hour and transferred onto a 0.2 μm Amersham™ Portran™ nitrocellulose membrane at 400 mA for 50 minutes on ice ...
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bioRxiv - Bioengineering 2023Quote: (GT)15-SWCNT-containing (1.0 mg/L) alginate-acrylamide gels were prepared by modifying a previously published procedure.40 Sodium alginate (116.7 mg, Thermo Fisher Scientific) and n-isopropylacrylamide (1524.94 mg ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 20 µg of protein per sample was separated by electrophoresis on 4-12% Bis-Tris Plus Gels (Life Technologies), transferred to nitrocellulose ...
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bioRxiv - Microbiology 2023Quote: ... Gels have been stained with silver to observe the total protein profile or with Pierce™ Glycoprotein Staining Kit (Thermo Fisher) to detect glycoprotein sugar moieties according to the manufacturer’s instruction.
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bioRxiv - Immunology 2023Quote: ... Paired HC and LC sequences were amplified using nested PCR reactions and analyzed by 2% 96 E-gels (Thermo Fisher Scientific) followed by sequencing (Eton Bioscience ...
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bioRxiv - Plant Biology 2024Quote: ... Denatured samples were spun down at 5,000 xg for 3 minutes and supernatant was run on 4%-20% Bio-Rad 4%-20% Mini-PROTEAN TGX gels alongside a PageRuler Plus prestained protein ladder (Thermo Scientific). The proteins were then transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: ... Approximately 2.5 µg of protein was loaded into each well of precast 10% NuPAGE Bis-Tris gels (Invitrogen, Thermo Fisher Scientific). Each gel contained two control samples ...
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bioRxiv - Genetics 2024Quote: ... Approximately 2.5 µg of protein was loaded into each well of precast 10% NuPAGE Bis-Tris gels (Invitrogen, Thermo Fisher Scientific). Each gel contained two control samples ...
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bioRxiv - Cancer Biology 2024Quote: ... An amount of 20 µg of total protein was added to each lane of a 1.5 mm × 10 well NuPAGE 4–12% Bis-Tris Gel (Invitrogen, Cat#NP0335BPX). Loaded gels were run using 1× NuPAGE MES SDS Running Buffer at 90 volts for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The reactions were separated on a 6% precast DNA retardation gel in 0.5% Tris borate-EDTA buffer (TBE, Thermo Fisher Scientific) at 100 V for 1.5 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were boiled in loading dye (LiCOR #928-40004) and equal protein amounts for each sample were loaded onto NuPAGE™ 4-12% Bis-Tris SDS-PAGE gels (Invitrogen Cat#s WG1402BOX ...
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bioRxiv - Cell Biology 2024Quote: Samples from Co-IP experiments were resolved by SDS-PAGE in a gradient polyacrylamide gel Bolt™ 4-20% Bis-Tris Plus (Invitrogen). After running ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were boiled in Laemmli buffer (95°C, 10 min) and 20 µl of each sample were loaded on a 4-12% Nupage gel (Invitrogen, NP0336) to be revealed by WB ...
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bioRxiv - Biochemistry 2024Quote: Purified Sl-Enc1/His-Sl-Enc2 protein samples were analyzed via SDS-PAGE with a 14% Novex Tris-Glycine Gels (Thermo-Fisher Scientific). The SDS-PAGE gel was stained with Coomassie and the overexpressed bands at the correct molecular weights were excised and stored in water ...
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bioRxiv - Biochemistry 2024Quote: ... plasmid isolation and PCR product extraction from agarose gels were carried out using GeneJET kits (Thermo Fisher Scientific, USA, #K0503, #K0832). Site directed mutagenesis was carried out using Phusion™ Plus DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Large chunks of precipitates were removed then from the cell lysate samples before running the samples through NuPAGE 10% Bis-Tris protein gels (Invitrogen, NP0301). For blotting phosphatase-treated Nucleocapsid proteins ...
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bioRxiv - Biochemistry 2024Quote: ... Each tube was then filled with 200 μL (sufficient to cover the gel particles) of a 50 mM NH4HCO3 solution containing 2 μg of trypsin (Fisher Scientific) and incubated in a temperature-controlled shaker at 37 °C for approximately 20 hours ...
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bioRxiv - Neuroscience 2024Quote: ... PCR product size and quality were assessed by sample analysis by electrophoresis through 10% polyacrylamide gels with post-staining using SYBR Gold (Invitrogen, S11494) at this point and just before sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... The RNA quantity and quality was inspected on a 1.2% agarose gel and with a Multiscan® GO Microplate Spectrophotometer (Thermo Scientific). RNA integrity (RIN between 6.6-8.8 ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of protein lysates are denatured in Laemmli SDS buffer and were run on 4–20% Tris-Glycine gels (Invitrogen, XP04205BOX), and transferred to nitrocellulose membrane ...
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bioRxiv - Neuroscience 2024Quote: ... and run using Invitrogen buffers (BN2002, BN2001) as described (Ref: Nishihara).23 The gel was stained with Coomassie to image the NativeMark ladder (Thermofisher, #LC0275), then destained and transferred onto a PVDF membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysate supernatants were quantified for protein concentration using the BCA Protein Assay Kit (TermoFisher Scientific, 23225) and separated on 4-20% Tris-Glycine gels (NovexTM, Life Technologies). In the case of WNK1 ...
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bioRxiv - Bioengineering 2024Quote: ... Sample proteins were then separated via SDS-PAGE and transferred to nitrocellulose membranes using the iBlot 2 Gel Transfer (Invitrogen, IB21001). Membranes were blocked with Odyssey Blocking Buffer (LICOR 927-60001 ...
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bioRxiv - Biochemistry 2024Quote: ... Efficacy of the process and final protein purity was assessed by SDS-PAGE using NuPAGE 4-12 % Bis-Tris Midi Protein Gels (ThermoFisher Scientific) and mass spectrometry ...
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bioRxiv - Biochemistry 2024Quote: ... Each tube was then filled with 200 μL (sufficient to cover the gel particles) of a 50 mM NH4HCO3 solution containing 2 μg of trypsin (Fisher Scientific) and incubated in a temperature-controlled shaker at 37 °C for approximately 20 hours ...
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bioRxiv - Biophysics 2024Quote: ... Native PAGE or SDS PAGE gels were transferred to a nitrocellulose membrane using an iBlot2 (20 V, 7 min; Invitrogen™). The membrane was incubated with blocking buffer (PBS ...
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bioRxiv - Biophysics 2024Quote: ... protein fractions were mixed 1: 1 with SDS loading buffer and loaded on a NuPageTM 4-12% Bis-Tris PAGE Gel (Invitrogen™) along with Novex™ Sharp Pre-stained Protein Standards (range from 3.5 to 260 kDa ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were incubated for 10 min at 95 °C and were immediately loaded in 4%–12% Bis-Tris Plus Gels (Invitrogen, Thermo Scientific) and ran at constant 120 V (see below).
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bioRxiv - Biochemistry 2024Quote: ... 20 μg of total protein (from whole cell extracts or isolated lysosomes) was separated with a 4%–12% Bis-Tris Plus Gels (Invitrogen, Thermo Scientific) and then transferred onto PVDF (polyvinylidene difluoride ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured by boiling for 15 minutes and separated on a 15-well Novex WedgeWell 4-20% Tris-Glycine Gel (Thermo Scientific) before transfer to a 0.22µm PVDF membrane overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Protein isolates were mixed with protein loading buffer containing 100 mM DTT reducing agent and denatured for 5 min at 95°C before loading onto 4–12% Bis-Tris NuPAGE precast gels (Thermo Fisher) for electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... and denatured at 70°C for 10 min prior to loading onto Novex NuPAGE 10% Bis-Tris Protein gels (Invitrogen, NP0315BOX) and separated half way (15min at 200 constant voltage) ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified DNAs from excised gel slices were cloned into a pCR4Blunt-TOPO vector using a Zero Blunt TOPO PCR Cloning Kit for Sequencing (ThermoFisher Scientific) and subjected to Sanger DNA sequencing using M13 forward and M13 reverse primers (Eurofins Genomics).
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bioRxiv - Microbiology 2024Quote: ... 0.25% Orange G w/v in water) before loading on to a 0.7% agarose gel in TAE buffer containing SybrSafe dye (ThermoFisher Scientific, S33102). DNA bands were visualized over blue light and a clean razor was used to cut out gel portions containing bands greater than 2 kb in length ...