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Citations for Thermo Fisher :
8601 - 8650 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific) cultured for 4-7 days at 8% CO2 saturation and 37°C with shaking ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was then incubated with a secondary antibody: goat anti-rabbit IgG (H+L) antibody (1:5000; Thermo Fisher Scientific) or Goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... E3.5 embryos were plated individually into gelatine-coated wells of 24-well plates in embryonic stem cell (ESC) medium (DMEM, high glucose 4500 mg/L (Gibco, ThermoFisher, #11965,), 100 µM 2-mercapto-ethanol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were seeded onto poly-L-lysine (PLL)-coated 12-well plates in DMEM supplemented with 10% FBS and transfected using the RNAiMAX reagent (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: BP were dissected in cold L-15 media and incubated for 1 hour at room temperature with 5 µM pHrodo Red Intracellular pH Indicator (Invitrogen P35372) and 1 nM SiR-actin (SpiroChrome SC001 ...
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bioRxiv - Microbiology 2023Quote: ... N (75 fmol) and L (25 fmol) proteins and the T7 RNA polymerase (50 fmol) using lipofectamine 3000 (Invitrogen™ L3000150) for 3 h at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... patens gametophores were grown on KNOP ME agar plates (12 g l−1 purified agar; Oxoid, Thermo Scientific, Waltham, MA, USA). Light intensity in growth cabinets was set to 70-100 µmol photons m−2 s−1 and 16:8 h light/dark cycle at 22 °C ...
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bioRxiv - Plant Biology 2023Quote: ... Each sample was ligated with a different L#clip2.0 adapter (see Supplementary Data 12) and purified again with the DynaBeads MyONE Silane kit (Thermo Fisher, 37002D). cDNAs were PCR pre-amplified — 98°C for 30 s (1X) ...
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bioRxiv - Biochemistry 2023Quote: ... Plates were then washed 4x in TBST and 30 µL of 1:5,000 Goat anti-mouse IgG (H+L) Secondary Antibody HRP (ThermoFisher 62-6520) were added to each well and incubated at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa tet-on shDCAF1 and U2OS cells (ATCC HTB-96) were cultured in Dulbeccós modified Eaglés medium containing 4.5 g/l glucose (Gibco, catalog no.: 41965-039) supplemented with 10 % fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2023Quote: ... supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Immunology 2023Quote: ... were coated with 50μl/well of RBD monomer at a concentration of 2 μg/ml in Dulbecco’s phosphate buffered saline (DPBS; Gibco Life Technologies). Plates were incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... was incubated overnight at 4°C and Alexa Fluor 488 goat anti-chicken IgY (H+L) secondary antibody (1:100, A-11039, ThermoFisher Scientific) was applied to detect GFP+ cells ...
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bioRxiv - Biochemistry 2023Quote: ... fresh P3 virus from γ-TuRC-GFP and γ-TuSC were mixed in a 1:1 ratio and used to infect 1.8-2 L of High five cells (Thermo Fisher Scientific) at a density of 3-3.5 x106 cells/mL by adding 30 mL of the γ-TuRC-GFP and γ-TuSC virus mixture as above ...
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bioRxiv - Bioengineering 2023Quote: ... 20 µL of 1:100 diluted fluorescent beads in DI water (FluoSpheres carboxylate-modified 0.2 µm, 660/680, F8807, Thermo Fisher Scientific), 6 µL 10% v/v tetramethylethylenediamine (TEMED ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were added to a 96-well PCR plate (10 µL/well) and subjected to heat challenge at temperatures as indicated for 3 minutes using a Veriti Thermal Cycler (Applied Biosystems) with variable temperature zones ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... HEK293T cells (ATCC® CRL-1573™) were cultured in DMEM plus L-glutamine (high glucose) supplemented by 10% FBS (Gibco) and penicillin/streptomycin (Gibco) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Five μL of each PCR product was mixed with loading dye and run on a 1 % agarose gel (SYBR Safe; Life Technologies) at 90 V for ∼45 min ...
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bioRxiv - Immunology 2023Quote: ... supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific), cultured for 4-7 days at 8% CO2 saturation and 37°C with shaking ...
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bioRxiv - Immunology 2023Quote: ... supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific), cultured for 4-7 days at 8% CO2 saturation and 37°C with shaking ...
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bioRxiv - Immunology 2023Quote: Antigen protein (100 µL; 10 µg/mL) in carbonic buffer (50 mM; pH 9.5) was immobilized on Maxisorp 96-well plates (Thermo Fisher Scientific) and incubated at 4 °C overnight ...
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Wild and cultivated rice host different populations of the blast fungus, Pyricularia oryzae, in MalibioRxiv - Microbiology 2023Quote: ... 1.5 μL of amplified products (1/70 dilution) were mixed with 15 μL Formamide HiDi and GeneScan-500LIZ size marker (Applied Biosystems). Amplifications were repeated twice and control strains were included in all amplification plates to verify calibration ...
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bioRxiv - Microbiology 2023Quote: ... A 5μl volume of this inoculum was then added in triplicate to a 96-well plate (BioLite 96 Well Multidish—Thermo Scientific) with wells each containing 200 ml of TSB+YE (control) ...
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bioRxiv - Immunology 2023Quote: HeLa-H2B-eGFP and MRC5 (ATCC CCL-171) were maintained as monolayers in Dulbecco’s modified Eagle’s medium (DMEM) (1 g/L glucose) (Gibco, Billings, MT, USA) supplemented with 10% heat-inactivated FBS and Pen–Strep.
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bioRxiv - Microbiology 2023Quote: ... The prepared cDNA (0.25 µl) were used for quantitative real time-PCR using 2x sybr green master mix (cat no.4344463, applied biosystems/Thermofisher scientific) to measure the amount of CHPV-N specific mRNA in infected Vero cells using CHPV-N specific primers (forward 5′-ACCTGGCTCCAAATCCAATAC-3′ and reverse 5′-GGTGGATCAGACGGAGAGATA-3′) ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed and then incubated with Horseradish peroxidase secondary antibodies against the last antibody: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen ® ...
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bioRxiv - Cell Biology 2023Quote: ... followed by PBS-Tween washes and secondary antibody incubation (2h at room temperature): Horseradish peroxidase secondary antibodies: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen® ...
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bioRxiv - Bioengineering 2023Quote: ... 2 µL of Lipofectamine™ 2000 were added to a final volume of 25μL of Opti-MEM™ Reduced Serum Medium (ThermoFisher). Then Cas9 RNP solution was added to Opti-MEM/lipid solution ...
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bioRxiv - Neuroscience 2023Quote: ... 1X N-2 (Thermo Fisher Scientific, 17502-048], 1X B-27 [Thermo Fisher Scientific, 17504-044], 1mM L-Glutamine [Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Neurons were then plated onto 18mm glass coverslips coated with 1 mg ml-1 Poly-L-Lysine (Dissolved in borate buffer, pH 8.5) and maintained in culture media composed of Neurobasal-A medium (Gibco/Thermo Fisher), 0.1% B27 supplement (Gibco/Thermo Fisher) ...
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bioRxiv - Bioengineering 2023Quote: (GT)15-SWCNT-containing (1.0 mg/L) alginate-acrylamide gels were prepared by modifying a previously published procedure.40 Sodium alginate (116.7 mg, Thermo Fisher Scientific) and n-isopropylacrylamide (1524.94 mg ...
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bioRxiv - Microbiology 2023Quote: ... 2.0×106 cells were fixed in 2% paraformaldehyde in PBS for 10 min at room temperature (RT) onto poly L-lysine treated 12 mm glass coverslips (Fisher Scientific). The coverslips were washed three times in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... 3D iHeps (Day 22) were incubated for 2 hours with HCM media containing 1μmol/L of 6-CDF (Thermofisher, #C-369). Samples were then washed with PBS for 15 minutes before staining with Hoescht in HCM.
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bioRxiv - Cell Biology 2024Quote: ... was amplified by creating single colonies of Escherichia coli in agar plates and incubated in Lennox L Broth (LB Broth; Invitrogen, UK). The pDNA was stored as glycerol stocks and purified using QIAprep spin miniprep kit (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with 1.0 or 10 µg/mL of an AF 488 conjugated Dk anti-Ms IgG (H+L) Ab (A21202, Thermo Fisher Scientific) for 4 h ...
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bioRxiv - Neuroscience 2024Quote: ... The number of Aβ plaques decorated with an AF 488 Dk anti-Ms IgG (H+L) Ab (A21202, Thermo Fisher Scientific) among total Aβ plaques was counted ...
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bioRxiv - Cancer Biology 2024Quote: ... EFM19, EFM192A, HCC1428, T47D, ZR751 & ZR7530 were cultured in in RPMI 1640 Medium (no phenol red, no L-glutamine) (Gibco # 32404014). The following supplements were added ...
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bioRxiv - Cancer Biology 2024Quote: ... A172 cell line was cultured in high glucose DMEM media plus 4 mM L-glutamate and 1 mM sodium pyruvate (Thermo Fisher) supplemented with 10% FBS and 1% penicillin and streptomycin mixture ...
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bioRxiv - Bioengineering 2024Quote: ... 100 μL of cell-containing hydrogels were added to the bottom of a well in 8-well LabTek chamber (#155360, Thermo Scientific). After 30 minutes ...
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bioRxiv - Genomics 2024Quote: ... qPCR was performed in a total volume of 15 μL (6 µL purified DNA or phage particle dilution and 9 µL of amplification mix) in MicroAmp Fast Optical 96-well plates sealed with MicroAmp Optical Adhesive Film (both from Applied Biosystems, reference 4346906 and 4311971 ...
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bioRxiv - Biochemistry 2024Quote: ... in 300 µl and the sample aliquots of 100 μl were heated at individual temperatures ranging from 20-80°C for 10 min using a PCR thermocycler (Veriti, Applied Biosystems). Heat-denatured material was pelleted at 5000 × g for 30 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... and HL-60 cells (ATCC® CCL-240) were grown in DMEM culture medium containing 1.0 g/l D-glucose (Gibco, 21885), supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used: goat anti-mouse IgG (H + L) highly cross-adsorbed secondary antibody Alexa Fluor 488 (Invitrogen/ThermoFisher), and goat anti-rabbit IgG (H + L ...
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bioRxiv - Microbiology 2024Quote: ... 100 µl of diluted phage and 300 µl of bacterial culture at OD600 between 0.4 and 0.5 as measured on a Genesys 30 Spectrophotometer (Thermo Scientific, Waltham, USA) were added to 3 ml of molten LM17-CaCl 0.75% w·v-1 agar and poured over LM17 1% w ·v-1 agar plates ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells were immobilized in 8-well poly L-lysine-coated IBIDI chambers and stimulated by the addition of CD3/28 Dynabeads (cat. 11452D, Thermo Fisher) for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed three times with 1X PBT for 5 minutes each and then incubated for 1 h at room temperature with secondary antibody (goat anti-rabbit IgG (H+L) Alexa Flour 546 (Invitrogen, A11035) or goat anti-mouse IgG (H + L ...
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bioRxiv - Neuroscience 2024Quote: ... and the secondary antibody used was a goat anti-mouse IgG (H+L) with either an Alexa Fluor 488 (1:250; Invitrogen A11029) or Alexa Fluor 647 congugate (1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 μg/mL Penicilin-Streptomicin) supplemented with 2 mM L-Glutamine (ref.07100, Stemcell) and 10 mM 2-Mercaptoethanol (ref.11528926, Thermo Fisher). The medium was partially changed every other day extremely slowly to prevent disturbing the EB formation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HEK293T cells were purchased from the ATCC and cultured in DMEM supplemented with L-glutamine and 10% fetal bovine serum (Life Technologies) at 37°C and 5% CO2 ...
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bioRxiv - Physiology 2024Quote: ... then incubated at RT for 2 h with goat anti-Rabbit IgG (H+L) Alexa Fluor Plus 488 (ThermoFisher, cat#A32731) and goat anti-guinea pig IgG (H+L ...