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Citations for Thermo Fisher :
8551 - 8600 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Eluted samples were run on 10% SDS-PAGE and the gel was stained with SimplyBlue SafeStain (ThermoFisher Scientific, cat. no. LC6065). For the digestion ...
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bioRxiv - Bioengineering 2023Quote: ... The conjugates were further incubated with 1.1:1 Cyanine 5 trans-cyclooctene (AAT Bioquest):Met-NHS for a minute and run through NuPAGE 4-12% Bis-Tris premade polyacrylamide gel (Invitrogen by Thermo Fisher Scientific). The mini gel tank (Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately 15 – 20 μg of mitochondria or 70 μg cellular protein were subjected to NuPAGE Novex 4 – 12% Bis-Tris gels (Life Technologies) according to manufacturer’s instructions and electro-transferred to polyvinyl difluoride membranes (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... the His-tagged target protein was eluted with 100% elution buffer at 1 mL min−1 flow rate and the collected fractions checked for recombinant ZNF524 expression on 4-12% NuPAGE Novex Bis-Tris precast gels (Thermo Scientific). His-MBP-ZNF524 of about 75% purity was sent to Pineda Antikörper-Service ...
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bioRxiv - Cancer Biology 2023Quote: ... and the size of the amplicon was checked by electrophoresis of the PCR product on an agarose gel in TAE 1X (Life Technologies) (data not shown) ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of total protein (30 to 50 μg) were separated in a 4–12% Bis-Tris polyacrylamide gels (Invitrogen, #NP03354) and transferred to a 0.22 μm nitrocellulose membrane (BIO-RAD ...
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bioRxiv - Genetics 2023Quote: ... the denatured nuclear extract was subjected to electrophoresis on a precast 3-8% Tris-Acetate polyacrylamide gel (Thermo Fisher Scientific, EA0378BOX) in 1× Tris-Acetate SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... The supernatants were quantified using the BCA kit (Pierce) and ∼10-30 µg total protein subjected to electrophoresis using NuPAGE 12% Bis-Tris gel in MES buffer (Thermo Fisher). The proteins were transferred to a 0.2-micron nitrocellulose membrane using a XCell II blot module (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... 20 μl of input and 30 μl of co-IP samples were loaded onto a 12% NuPAGE™ Bis-Tris gel (Invitrogen) and separated at 200 V for 40 min ...
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bioRxiv - Microbiology 2022Quote: ... Guide RNAs were purified using a 2X SPRI reaction and checked for purity by running on a 15% TBE-Urea Gel (Invitrogen EC6885BOX). Guide RNA concentration was quantified using the Broad Range RNA Qubit kit (Thermo Fisher Scientific Q10210).
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bioRxiv - Molecular Biology 2023Quote: ... 20 μl was loaded on to NuPAGE 4 to 12% Protein Gels with 7 μl of 4X NuPAGE LDS sample buffer as described by the manufacturer (Invitrogen: NP0322BOX) and run for 2 h at 100 V ...
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bioRxiv - Cell Biology 2023Quote: ... small sections of the larger gel were cut and gently dried before being placed into 35 mm #1.5 coverslips bottomed imaging dishes (Cellvis; Fisher Scientific #NC0409658) precoated with poly-D-lysine ...
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bioRxiv - Bioengineering 2023Quote: ... and purity were estimated using agarose gel electrophoresis (1%) and with a NanodropTM 2000 UV-Vis spectrophotometer (Thermo Scientific, Waltham, USA).
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bioRxiv - Neuroscience 2023Quote: ... Cell lysates were combined with NuPAGE LDS Sample Buffer (Novex #1249698) and run directly on Invitrogen Blot 4 – 12% Bis-Tris Protein Gel (Invitrogen #NW04125BOX) in 1XMOPS solution ...
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bioRxiv - Systems Biology 2023Quote: ... The quality of total DNA was measured by 1% agarose gel electrophoresis and Nano drop 8000 Spectrophotometer (Thermo Scientific, Waltham, MA) (260 nm/280 nm ratio) ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting supernatant was mixed with 0.25% G-250 Sample Additive and loaded on a NativePAGE 3-12% Bis-Tris gel (BN1001BOX, Invitrogen, CA, USA) for electrophoresis.
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bioRxiv - Cancer Biology 2023Quote: ... 20 mg of total protein was loaded for each sample into NuPAGE™ 4-12% Bis-Tris gels (Thermo Fisher Sci.) and separated electrophoretically in NuPage LDS Running Buffer (Thermo Fisher Sci. ...
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bioRxiv - Bioengineering 2023Quote: VHH proteins were incubated with enteric proteases for indicated times and resolved on a 4–20% Novex™ Tris/Glycine SDS/PAGE gel (Invitrogen). The gel was then electroblotted onto PVDF membrane and visualized with 0.02% Coomassie Brilliant blue in 40% methanol ...
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bioRxiv - Genetics 2023Quote: ... The quality of the DNA was assessed visually by electrophoresis through 0.7% agarose gel and the amount quantified with a QubitTM following the manufacturer’s instructions (Thermo Fisher Scientific Inc.). DNA extractions were adjusted to a final concentration at 10ng DNA/μL.
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bioRxiv - Neuroscience 2023Quote: ... 7-10 μg (cultured astrocyte lysates) of protein was loaded into Bolt™ 4–12% Bus-Tris Plus gels (Thermo Scientific) and run at 150 V for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... The T7-sgRNA PCR product was purified using Gel Extraction Kit (Omega) and used as the template for IVT of sgRNAs using the MEGAshortscript T7 kit (Life Technologies). Both the Cas9 mRNA and sgRNAs were purified using the MEGAclear kit (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... The 10 μg of samples and the PageRuler Plus Prestained Protein Ladder (Protein Biology, Cat#26619) were then loaded onto a NuPAGE™ 4-12% Bis-Tris Gel with 12 wells (Invitrogen). The gel was run with 1X NuPAGE™ MOPS SDS Running Buffer (20X ...
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bioRxiv - Neuroscience 2022Quote: ... The gel was fixed in 40% methanol and 10% acetic acid and subsequently stained for 1h using colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Fisher). Each lane from the gel was cut in 3 gel pieces and placed in 0.5-mL tubes ...
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bioRxiv - Bioengineering 2022Quote: All vectors were denatured at 100°C for 5 min using Laemmli sample buffer and separated by SDS-PAGE on 10% Tris-glycine polyacrylamide gels (Life Technologies). Precision Plus Protein All Blue Standards (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... The beads were again separated using a magnetic rack and the entire volume was then loaded onto a precast 10-well 4-12% Bis-/Tris NuPAGE gel to separate the proteins for 45 min and 180 V in 1x MES buffer (Thermo Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... 10µg of protein was loaded per well and run on a 4- 12% bis-tris precast SDS page gel (Invitrogen, NP0323BOX). Semi-dry transfer was performed using the TransBlot Turbo transfer system (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... alkylated with IAA at RT for 45 min and loaded in a NuPAGE® 4-12% Bis-Tris Gel 1.0 mm (Life Technologies). After a migration of 20 min at 200 V ...
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bioRxiv - Cell Biology 2023Quote: ... digitonin-solubilized (4 g/g) mitochondria (10–50 μg) were loaded in NativePAGE Novex 3–12% (vol/vol) gels (Life Technologies). After electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... Specificity and concentration of purified PCR products were checked on 1% agarose gel and with a NanoDrop One© spectrophotometer (ThermoFisher), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... to a final detergent:dye ratio of 16 g/g before running on a 3-12% NativePAGE Mini Protein Gel (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 15 µg of total protein was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a 10-20% tris-glycine gradient (Invitrogen, XP10205BOX), transferred to a PVDF membrane using the Trans-Blot Turbo Transfer System (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... After elution from the column as described above, the RNA is combined with 12 μL of Gel Loading Buffer II (GLBII, 95% formamide (ThermoFisher Scientific), 18 mM EDTA (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Protein sample (10-15 µg) was resolved in a 12% SDS-PAGE gel and transferred to a PVDF transfer membrane (Thermo Scientific). The membrane was blocked for 15 minutes in buffer containing 50 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were spun at top speed in a tabletop centrifuge and quantified by Qubit and BCA protein quantification assay before loading in a NuPage 4-12% Bis-Tris gel (ThermoFisher NP0322BOX) and subjected to electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... The optimal amplification cycle (defined as the cycle before the PCR reaction reaching a plateau) was preliminarily determined by a diagnostic PCR visualized on a 10% TBE polyacrylamide gel (Invitrogen, EC6275BOX). PCR products of miRNAs (expected size range ...
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bioRxiv - Plant Biology 2023Quote: ... The amplified PCR product gel-extracted and gateway cloned into the pDONR207 entry clone with the BP Clonase II Enzyme Mix (ThermoFisher Scientific). Using the resulting pDONR207-RKF1 entry vector ...
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bioRxiv - Plant Biology 2023Quote: ... The RNA 5’ ends were radiolabeled using [γ-32P]-ATP and polynucleotide kinase and the covalently-linked protein-RNA complexes separated on a 4-12% NuPAGE Bis-Tris gel (Thermo Scientific) before transfer onto a nitrocellulose membrane ...
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bioRxiv - Physiology 2023Quote: ... We performed electrophoresis with 20 μg/lane of each lysate in Bolt 4–12% Bis-Tris Plus Gels (Thermo Fisher Scientific). We transferred the protein samples onto polyvinylidene difluoride membranes with the iBlot2 Dry Blotting System (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... Denatured samples were spun down at 5,000 g for 3 min and supernatant was run on 4–20% Bio-Rad 4–20% Mini-PROTEAN TGX gels alongside a PageRuler Plus prestained protein ladder (Thermo Scientific). The proteins were then transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad ...
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Comparative Proteomic Study of Retinal Ganglion Cells Undergoing Various Types of Cellular StressorsbioRxiv - Molecular Biology 2023Quote: ... and reduced with DTT then denatured at 70°C for 10min prior to loading 20μg onto Novex NuPAGE 10% Bis-Tris Protein gels (Invitrogen, Cat.# NP0315BOX) and separated appropriately (@ 200 constant V) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Successful extractions were verified by visualizing DNA using gel electrophoresis as above and quantified using a NanoDrop 2000 (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: Cell lysates and pull-down samples were analysed on 4-12% bis-tris SDS-PAGE gels and transferred onto nitrocellulose membrane using iBlot2 (Invitrogen, IB21001). Membranes were treated in 5% non-fat milk blocking solution for 1hr and incubated with primary antibodies listed in Suppl ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gels were then rinsed one time in deionized water and placed into an iBlot 2 mini transfer stack (Thermo Fisher Scientific). The transfer stack was loaded onto the iBlot 2 Gel Transfer Device (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... The purity of the recombinant proteins was checked by SDS–PAGE gels and verified by a Voyager STR matrix-assisted laser desorption ionization time-of-flight-mass spectrometer (Applied Biosystems), as well as NMR assignments ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a BCA protein assay kit (Pierce) and 15-20 μg of protein was separated on a 4-12% NuPAGE Bis-Tris gel (Life Technologies) followed by transfer to PVDF membranes ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 25 µg for each sample was loaded on a NuPAGE 4-12% Bis-Tris gel with 1X NuPAGE MOPS Running buffer (Life Technologies, Invitrogen) and separated for 1.5 h at 120V ...
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bioRxiv - Bioengineering 2023Quote: ... The gels were immediately imaged on an EVOS FL Auto microscope (4X LPlan PH2 objective, NA: 0.13, Life Technologies, Carlsbad, CA).
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bioRxiv - Synthetic Biology 2023Quote: ... The samples were run through the SDS gel for 2 hours at 120 Volts and then transferred to a polyvinylidene difluoride (PVDF) membrane (Thermo Scientific) via an electroblotting system (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... Protein extracts were resolved on a 12% SDS-PAGE gel and transferred to a nitrocellulose membrane using the iBlot® (Invitrogen) gel transfer device ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins/complex were subject to SDS-PAGE electrophoresis followed by protein staining (SYPRO Ruby Protein Gel Stain, Thermo Fisher Scientific) and immunoblotting.