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Citations for Thermo Fisher :
8551 - 8600 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Dissociated rat hippocampal cells were plated on 25 mm poly-l-lysine-coated glass coverslips and cultured at 37 °C with 5% CO2 in neurobasal media (ThermoFisher, 21103049) supplemented with B27 (GIBCO) ...
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bioRxiv - Neuroscience 2023Quote: Early postnatal P1 or P6 murine cochleae were dissected as a single piece in Leibovitz’s L-15 culture medium (21083-027, Thermo Fisher Scientific) and then fixed in 4% PFA in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Neuroscience 2023Quote: ... Early postnatal (P3-5) murine cochleae were dissected as a single piece in Leibovitz’s L-15 culture medium (21083-027, Thermo Fisher Scientific) then fixed in 4% formaldehyde for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... The conditioned organoid media collected from small intestinal organoids before passaging comprised of Advanced DMEM/F12 supplemented with 1x L-Glutamine (Life Technologies), 10 mM HEPES buffer (Life Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... An aliquot (25□l) of this lysate was used for protein quantitation using Pierce Coomassie protein assay kit (cat#23200, ThermoFisher scientific). The remaining lysate was centrifuged at 5000xg at 4C to pellet debris ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated for 2 h at room temperature in secondary antibody goat anti-rabbit IgG (H+L) Alexa 647 (Invitrogen, A32733) diluted 1:500 in PB 0.1 M containing 0.3% Triton X-100 and 3% NGS.
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bioRxiv - Neuroscience 2023Quote: ... we washed 5 times with PB 0.1 M and incubated them for 1 h at room temperature in secondary antibody goat anti-rabbit IgG (H+L) Alexa 647 (Invitrogen, A32733) diluted 1:1000 in PB 0.1 M containing 1% NGS ...
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bioRxiv - Molecular Biology 2023Quote: Cell pellets were re-suspended in 240 µl PBS and 9 μl 10% triton X-100 containing 0.3 µl of 1 mg/ml Streptavidin conjugated with Alexa Fluor® 647 (Life technologies) and 0.6 μl of 1 mg/ml Wheat Germ Agglutinin (WGA ...
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bioRxiv - Immunology 2023Quote: ... and 4T1 (obtained from AstraZeneca) mammary carcinoma cells were cultured in DMEM supplemented with 2mM L-glutamine (#21875034 Thermo Fisher Scientific), 10% FBS (#F9665 Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2023Quote: A 50 µL of dodecane overlay was extracted from each culture media and analysed by GC–MS using Trace 1300 GC (ThermoFisher Scientific), equipped with TraceGOLD TG-5MS (30 m[×[0.25 mm[×[0.25 μm) ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were incubated with the secondary antibodies goat α-rabbit IgG (H&L) Alexa Fluor 488 (1:250, Invitrogen A11008) and goat α-mouse IgG (H&L ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transfected with the TRIB1 targeting (L-003633-00-0010) or non-targeting control (D- 001810-10-2) ON-TARGETplus siRNA SMARTpools (Dharmacon) using RNAiMAX (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Peptides were resuspended in 2% acetonitrile/0.1% trifluoroacetic acid to a volume of 30 µl and 2 µL of protein digest was analyzed using nanoflow liquid chromatography on a Dionex UltiMate 3000 system (Thermo Scientific) coupled to a Q Exactive Plus mass spectrometer (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... 1:100 fold diluted Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor™ 488, ThermoFisher, Cat.#R37116) was then added and incubated at room temperature for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... Anoxic plate cultivation was done in 3.5 L anaerobic jars with Oxoid™ AnaeroGen™ gas generation sachets (Thermo Fisher Scientific) to remove oxygen to yield levels < 1% ...
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bioRxiv - Genomics 2023Quote: ... and then cultured in Neural Crest Differentiation Medium (50%-50% v/v mixture of DMEM/F12 1:1 medium with L-glutamine, without HEPES (Cytiva, SH30271.FS) and Neurobasal medium (Gibco, 21103049) with 0.5x N2 NeuroPlex (Gemini Bio ...
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bioRxiv - Cell Biology 2023Quote: ... dried peptides were resuspended in 50 μL of 100 mM TEAB pH 8.5 and labeled using two tags from the TMT10plex Isobaric Labeling Kit (Thermo Fisher Scientific) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was then incubated with a secondary antibody: goat anti-rabbit IgG (H+L) antibody (1:5000; Thermo Fisher Scientific) or Goat anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific) cultured for 4-7 days at 8% CO2 saturation and 37°C with shaking ...
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bioRxiv - Genomics 2023Quote: ... All cells were maintained at 37 °C and 5% CO2 in high glucose DMEM medium supplemented with L-Glutamine and Sodium Pyruvate (Gibco 11995065) and 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: ... The first-strand synthesis reactions were performed in 10 µl total volume with 5 µl of RT Buffer and 0.125 µl of Maxima H minus Reverse Transcriptase (Thermofisher Scientific, #EP0753) and 1 µl of 10 μM template switch oligo (TSO ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody HRP conjugated (31460) and goat anti-mouse IgG (H+L) secondary antibody HRP conjugated (31430) were from Thermo Scientific and rabbit anti-ATP13A3 antibody (HPA029471 ...
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bioRxiv - Genetics 2022Quote: ... Cells were passaged twice per week following a 5-min incubation in PBS containing 0.2 g/L EDTA (Invitrogen, Carlsbad, CA).
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibody staining was performed using goat anti-mouse polyclonal IgG (H+L) AlexaFluor™ 647 (Invitrogen A-20990, 1:1000) for 2 hrs ...
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bioRxiv - Bioengineering 2023Quote: ... The harvested cells incubated for 30 min or 4 h were spin washed with PBS and stained with LIVE/DEAD™ Fixable Violet Dead Cell Stain dye (L/D violet) (Invitrogen) diluted in PBS (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: The resulting construct (OR51E2-miniGs399) was transfected into 1 L of inducible Expi293F-TetR cells (unauthenticated and untested for mycoplasma contamination, Thermo Fisher) using the ExpiFectamine 293 Transfection Kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... 1X Phosphate Buffered Saline (PBS) and standard growth media was replaced with phenol red-free Leibovitz’s L-15 medium (Thermo Fisher Scientific), supplemented with 20% FBS and 1% penicillin-streptomycin.
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bioRxiv - Cell Biology 2023Quote: ... then cells were homogenized and seeded on poly-L-lysine coated #1.5H glass coverslips to a density of 4000 cells per cm2 in MEM (ThermoFisher #21090-055) supplemented with fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: The expression of NPP-10N N-terminally fused to MBP was induced by the addition of 1 mM of isopropyl β-D-thiogalactopyranoside (IPTG) to 1 L Luria Broth cultures of exponentially growing Escherichia coli BL21 DE3 pLysS (Invitrogen) strain (OD = 0.6) ...
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bioRxiv - Cell Biology 2023Quote: The expression of NPP-19 full length N-terminally fused to GST was induced by the addition of 1 mM of isopropyl β-D-thiogalactopyranoside (IPTG) to 1 L Luria Broth cultures of exponentially growing Escherichia coli BL21 DE3 pLysS (Invitrogen) strain (OD = 0.6) ...
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bioRxiv - Plant Biology 2023Quote: ... 20 μl of input and 30 μl of co-IP samples were loaded onto a 12% NuPAGE™ Bis-Tris gel (Invitrogen) and separated at 200 V for 40 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies were captured by 20 µ l of a 50:50 mix of Protein-A and Protein-G Dynabeads (Life Technologies). The beads were washed several times sequentially in the buffers containing low salt (150 mM NaCl) ...
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bioRxiv - Bioengineering 2022Quote: ... 100 μL of AIM-V medium and 10 μL of the prepared cell suspension was added to each well (Thermo Scientific Nunc™ F96 MicroWell™ Polystyrene Plate ...
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bioRxiv - Systems Biology 2023Quote: ... 150 μL aliquots of infected cells were deposited in a white 96-well plate (F96 MicroWell™ Plates, non-treated form Nunc) and the luminescent signal was measured with the help of the plate reader.
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bioRxiv - Microbiology 2023Quote: ... King’s B (KB) medium was prepared by mixing 20 g/L of proteose peptone #3 (BactoTM Proteose Peptone No. 3, ThermoFisher Scientific), 1.5 g/L of dipotassium phosphate (Carl Roth GmbH + Co ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, 4.5 g/l glucose, Thermo Fisher Scientific AG, Waltham, MA, USA), supplemented with 10% FCS ...
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bioRxiv - Neuroscience 2023Quote: ... Labeled protein was filter-dialyzed against 10 L of PBS using a 3K-CutOff Slide-A-Lyzer™ Dialysis Cassette (ThermoFisher). Labeling quality was assessed by Mass Spectrometry as previously described [46].
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bioRxiv - Systems Biology 2023Quote: ... were maintained in Dulbecco’s Modified Eagle’s Media (DMEM, 4500 mg/L glucose, MilliporeSigma) supplemented with 10% (v/v) fetal bovine serum (FBS; Thermo Fisher Scientific). MCF-7 cells tagged with mVenus fluorescent protein at the endogenous p21 locus and transfected with DHB-mCherry and H2B-mTurquoise (a kind gift from Dr ...
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bioRxiv - Biochemistry 2023Quote: ... GH43A Humicola insolens Y1 β-xylosidase / α-l-arabinofuranosidase (HiXyl43A)32 and GH10 Xyn10A from Paenibacillus barengoltzii CAU904 xylanase (PbXyn10A)33 were ordered from GeneArt (ThermoFisher) and cloned into pET26b expression vector with a C-terminal hexahistidine tag by the Gibson assembly method ...
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bioRxiv - Cancer Biology 2023Quote: ... L-005139-00-0005) or a non-targeting control siRNA (Dharmacon, D-001810-10-05) by using Opti-MEM (Gibco, 31985062) and the TransIT-X2® Dynamic Delivery System (Mirus ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5 μL of the resulting sample was analyzed by LC-MS/MS using an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Scientific) connected to a nanoACQUITY UPLC system (Waters ...
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bioRxiv - Microbiology 2023Quote: ... The plates were washed three times with in PBST and 50 µL of HRP-conjugated goat anti-hamster IgG (H+L) cross-adsorbed secondary antibody (Invitrogen, HA6007) was added at 1:5000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated in click reaction buffer (10 mM sodium-l-ascorbate, 20 µM biotin azide [Life Technologies], and 2 mM CuSO4) at RT for 2 hours on a rotator ...
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bioRxiv - Cell Biology 2023Quote: ... L-003253-00-0005) or a non-targeting control (Dharmacon, D-001810-01-05) using Lipofectamine RNAiMAX Transfection Reagent (Life Technologies), according to the manufacturer’s procedures ...
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bioRxiv - Immunology 2023Quote: ... The following secondary antibodies were used at 1:300 dilution: Alexa Fluor 555 donkey anti-rabbit IgG (H+L) cross-adsorbed (ThermoFisher Scientific), Alexa Fluor 647 donkey anti-rat IgG (H+L ...
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bioRxiv - Biochemistry 2023Quote: ... Freshly isolated mitochondria were resuspended in 50 μl of click-it lysis buffer (50 mM TRIS-HCl pH 8, 1% SDS, 250 U/mL Universal Nuclease [Thermo Fisher]) and incubated on ice for 15 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... All eluted proteins were dialyzed against 5 L 1x PBS using SnakeSkin dialysis tubes with 3.5k molecular weight cut-off (MWCO) (Thermo Fisher Scientific) at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The virus was propagated in C6/36 Aedes albopictus cell line at a multiplicity of infection (MOI) of 0.01 and cultured for 6 days in Leibovitz’s L-15 medium (Thermo Fisher Scientific, 11415064) supplemented with 0.3% tryptose phosphate broth (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa tet-on shDCAF1 and U2OS cells (ATCC HTB-96) were cultured in Dulbeccós modified Eaglés medium containing 4.5 g/l glucose (Gibco, catalog no.: 41965-039) supplemented with 10 % fetal bovine serum (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... HEK293T cells (ATCC® CRL-1573™) were cultured in DMEM plus L-glutamine (high glucose) supplemented by 10% FBS (Gibco) and penicillin/streptomycin (Gibco) ...