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Citations for Thermo Fisher :
8501 - 8550 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: A Mettler-Toledo (Columbus, OH) Seven Easy model pH meter was used with a gel-filled pencil-thin pH electrode from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2023Quote: ... The remaining ∼10% of the cell lysate was then homogenized into a denaturing buffer solution containing 4X NuPAGE™ LDS Sample Buffer in preparation for polyacrylamide gel electrophoresis (Invitrogen™ NuPAGE™ ...
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bioRxiv - Molecular Biology 2023Quote: ... 10μg of total protein were heat-denatured for 5 minutes at 100°C before loading onto NuPAGE 3– 8% TRIS Acetate Midi Gel (Novex, Life Technologies) and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of whole protein extracts were loaded and separated by electrophoresis on a 4–12% Bis-Tris gel (Life Technologies) and transferred to a PVDF membrane (Thermo Fischer Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... alkylated with IAA at RT for 45 min and loaded in a NuPAGE® 4-12% Bis-Tris Gel 1.0 mm (Life Technologies). After a migration of 20 min at 200 V ...
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bioRxiv - Cell Biology 2023Quote: ... digitonin-solubilized (4 g/g) mitochondria (10–50 μg) were loaded in NativePAGE Novex 3–12% (vol/vol) gels (Life Technologies). After electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... Lysates containing 60ug of total protein were electrophoresed for 3 hrs at 40C and 100 V using 3%-12% Bis-Tris polyacrylamide gels (ThermoFisher, BN1001BOX) and a running buffer containing 15mM Bis-Tris ...
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bioRxiv - Cell Biology 2023Quote: ... After elution from the column as described above, the RNA is combined with 12 μL of Gel Loading Buffer II (GLBII, 95% formamide (ThermoFisher Scientific), 18 mM EDTA (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... Immunoblotting was performed according to standard procedures with the following modifications: a 3-8% tris acetate gel (Thermo Fisher Scientific, EA03752) was used and samples were run for 2 hours 15min at 80V at 4 °C in 1X tris-acetate running buffer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Eluted proteins and 50 μg of protein from whole cell lysate were resolved in 4-15% NuPAGE gradient gel (ThermoFisher, NP0335) using MOPS running buffer (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were sonicated (10x 5s on/off) and the samples were loaded onto SDS PAGE gel and transferred to PVDF membranes using the NuPAGE system (Life technologies) and visualized using SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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A new vulnerability to BET inhibition due to enhanced autophagy in BRCA2 deficient pancreatic cancerbioRxiv - Cancer Biology 2023Quote: ... Protein lysates were separated on 4% to 12% Bis-Tris NuPAGE gels and run with MOPS Running Buffer (Life Technologies, J62847), transferred to polyvinylidene difluoride membrane (Millipore ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... Total lysates (10-20 µg) were separated by SDS-PAGE on NuPAGE™ 4-12% Bis-Tris Midi Protein Gels (Invitrogen) or 3-8% Tris-Acetate CriterionTM XT Precast Gels (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... DNA migration was detected by a 5 min incubation with SYBR Gold Nucleic Acid Gel Stain (Invitrogen #S11494, 1/10, 000) and revealed using a Chemidoc MP Immaging System (BIO-RAD) ...
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bioRxiv - Molecular Biology 2023Quote: ... The immunoprecipitated proteins were run on 4-12 % SDS-PAGE gel by 1 cm from the well and stained with SimplyBlue (Thermo Fisher) for in-gel digestion ...
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bioRxiv - Neuroscience 2023Quote: TG tissue was extracted fresh and flash frozen on dry ice before being embedded in optimal cutting temperature gel (Cat. No. 23-730-571, Fisher Scientific). Tissue was sectioned at 20 µm on a cryostat at - 20°C and mounted directly onto SuperFrost Plus microscope slides (cat ...
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bioRxiv - Molecular Biology 2023Quote: ... The T7-sgRNA PCR product was gel-purified and employed as the template for IVT using a MEGAshortscript T7 kit (Life Technologies). Both the hCas9 mRNA and Rtl8a ...
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bioRxiv - Molecular Biology 2023Quote: ... The T7-Cas9 PCR product was gel purified and employed as the template for in vitro transcription (IVT) using a mMESSAGE mMACHINE T7 ULTRA kit (Life Technologies). The T7 promoter was added to the Rtl8a ...
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bioRxiv - Microbiology 2023Quote: ... to be separated on 8% SDS-polyacrylamide gel electrophoresis (PAGE) or subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) using Superscript IV (Invitrogen Life Technologies), to create cDNA and then DNA to amplify for sequencing.
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bioRxiv - Microbiology 2023Quote: PCR products were verified on a 2% agarose gel electrophoresis and the expected products were subsequently purified using ExoSAP-IT PCR Product Cleanup Reagent (Applied Biosystems) or Zymoclean Gel DNA Recovery Kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2023Quote: ... equal amounts of proteins were resolved by SDS-PAGE and transferred to nitrocellulose membrane (IBlot gel transfer, Life Technologies, California, USA). The membrane was saturated with 5% lyophilized milk in TBST and primary antibodies were incubated at 4°C overnight ...
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bioRxiv - Physiology 2023Quote: ... The gel was then rinsed with deionized water and fluorescence was visualized at 800 nm using an iBright Imaging System (Thermo Fisher).
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bioRxiv - Cell Biology 2023Quote: ... Lysates were normalized to the same protein concentration and run on precast 48-well E-PAGE gels (Thermo Fisher Scientific, #EP04808). Gels were transferred to nitrocellulose membranes using the iBlot semidry system (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in PBS and 1 μL of this cell suspension was placed onto a gel pad composed of 1.2% agarose (TopVision, Thermo Fisher Scientific) prepared in PBS and mounted on a microscopy slide.
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bioRxiv - Systems Biology 2023Quote: The PCR products (6 µl per well) were confirmed through 96-well E-gel with SYBR (Thermo Fisher, Catalog no G720801) using 25 µl of loading buffer (Thermo Fisher ...
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bioRxiv - Systems Biology 2023Quote: The PCR products (6 µl per well) were confirmed through 96-well E-gel with SYBR (Thermo Fisher, Catalog no G720801) using 25 µl of loading buffer (Thermo Fisher ...
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bioRxiv - Systems Biology 2023Quote: ... The samples were normalized for protein amounts and an equal amount of protein from all the samples were run on 4-12% bis-tris gels (Invitrogen, NP0336BOX). For the Far11 mobility shift assay ...
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bioRxiv - Immunology 2023Quote: ... then S-glutathionylated proteins were released and prepared for SDS polyacrylamide gel electrophoresis (SDS-PAGE) with reducing sample buffer at 100°C (ThermoFisher #39000); immunoblotting was performed with anti-PDCE2 antibodies (GeneTex #GTX109766) ...
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bioRxiv - Biophysics 2023Quote: ... NuPAGE® LDS Sample Buffer (4X) and NuPAGE®Novex 4-12% Bis-Tris Protein Gels (1.0 mm 10 well) were obtained from Life Technologies. Quick StartTM Bradford 1x Dye Reagent was from Bio-Raid ...
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bioRxiv - Biophysics 2023Quote: ... heated to 95 °C for 5 min and loaded onto the gel with PageRuler™ Prestained Protein Ladder (Thermo Fisher Scientific) as a reference marker ...
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bioRxiv - Developmental Biology 2023Quote: ... and 37 and 75 kDa were removed and further excised into small pieces 1.5 ml tube followed by in-gel digested using Tryptic Digestion Kit (Thermo Scientific, 89871) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The lysates (20 - 30 μg of total protein) were subjected to electrophoresis on NuPAGE 4-12% Bis-Tris gels (Invitrogen, NP0322BOX), transferred to PVDF membranes (BIO-RAD ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were subjected to a 4%-15% polyacrylamide SDS gel electrophoresis followed by electrotransfer to a nitrocellulose membrane (Fisher Scientific). The membrane was blocked with 3% milk ...
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bioRxiv - Immunology 2023Quote: ... Integrity and purity of purified proteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Coomassie G-250 staining (ThermoFisher Scientific). Purified proteins were stored at –80°C until use.
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bioRxiv - Molecular Biology 2023Quote: ... RNA fragments of appropriate size (28-32 nt) were obtained by running samples on 15% polyacrylamide denaturing TBE-Urea gel and visualized by SYBR Gold dye (Life Technologies). Size selected RNA was dephosphorylated by T4 polynucleotide kinase (PNK ...
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bioRxiv - Genetics 2023Quote: ... Equal amounts of protein (30-50 μg) were loaded on NuPAGE Bis-Tris 4%–12% gradient polyacrylamide gels in the MOPS or MES buffer system (ThermoFisher Scientific) and subsequently electrotransferred from mini gels to a solid nitrocellulose membrane using the iBlot 2 system (ThermoFisher Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The DNA integrity and purity was monitored on 1% agarose gels and by NanoDropTM One UVVis spectrophotometer (Thermo Fisher Scientific, USA). DNA concentration was further measured using Qubit® 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Thirty µg of cytosolic or 10µg of mitochondrial proteins from each sample were separated on NuPAGE® Novex® Bis-Tris 4–12% gels kit (Invitrogen) and transferred to PVDF membranes (Trans-blot® Turbo™ Transfer System ...
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bioRxiv - Cancer Biology 2023Quote: ... The reaction products were separated on denaturing polyacrylamide gel (15% w/v) and the DNA products were visualized and photographed using iBright FL 1500 (ThermoFisher Scientific). The band intensities in the gels were determined using ImageJ software (NIH).
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bioRxiv - Immunology 2023Quote: ... was added to a concentration of 1X and the total protein from each sample was resolved on NuPage 4–12% gels (Life Technologies), transferred to nitrocellulose membranes (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... boiled for 5 min at 95°C and separated on a 4-12% NuPAGE Novex Bis-Tris precast gel (Thermo Scientific).
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bioRxiv - Neuroscience 2023Quote: ... The optimal amplification cycle (defined as the cycle before the PCR reaction reaching a plateau) was preliminarily determined by a diagnostic PCR visualized on a 10% TBE polyacrylamide gel (Invitrogen, EC6275BOX). PCR products of miRNAs (expected size range ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were spun at top speed in a tabletop centrifuge and quantified by Qubit and BCA protein quantification assay before loading in a NuPage 4-12% Bis-Tris gel (ThermoFisher NP0322BOX) and subjected to electrophoresis ...
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bioRxiv - Biophysics 2023Quote: ... 20 µL of annealed origami samples were loaded onto the gel alongside a DNA reference ladder (100 bp GeneRuler, Thermo Scientific). Control samples included origami staples and annealed scaffold ...
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bioRxiv - Bioengineering 2023Quote: ... The conjugates were further incubated with 1.1:1 Cyanine 5 trans-cyclooctene (AAT Bioquest):Met-NHS for a minute and run through NuPAGE 4-12% Bis-Tris premade polyacrylamide gel (Invitrogen by Thermo Fisher Scientific). The mini gel tank (Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: Eluted samples were run on 10% SDS-PAGE and the gel was stained with SimplyBlue SafeStain (ThermoFisher Scientific, cat. no. LC6065). For the digestion ...
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bioRxiv - Bioengineering 2023Quote: ... The conjugates were further incubated with 1.1:1 Cyanine 5 trans-cyclooctene (AAT Bioquest):Met-NHS for a minute and run through NuPAGE 4-12% Bis-Tris premade polyacrylamide gel (Invitrogen by Thermo Fisher Scientific). The mini gel tank (Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately 15 – 20 μg of mitochondria or 70 μg cellular protein were subjected to NuPAGE Novex 4 – 12% Bis-Tris gels (Life Technologies) according to manufacturer’s instructions and electro-transferred to polyvinyl difluoride membranes (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... the His-tagged target protein was eluted with 100% elution buffer at 1 mL min−1 flow rate and the collected fractions checked for recombinant ZNF524 expression on 4-12% NuPAGE Novex Bis-Tris precast gels (Thermo Scientific). His-MBP-ZNF524 of about 75% purity was sent to Pineda Antikörper-Service ...
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bioRxiv - Cancer Biology 2023Quote: ... and the size of the amplicon was checked by electrophoresis of the PCR product on an agarose gel in TAE 1X (Life Technologies) (data not shown) ...