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Citations for Thermo Fisher :
8501 - 8550 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody (Alexa Fluor 546) (Cat. NO. A11003) were from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2024Quote: ... and subsequently incubated for 30 min with secondary antibodies (Alexa Fluor goat anti-mousse IgG (H+L)/Alexa Fluor 647 secondary antibody (Invitrogen, A21236) at 1:1000 ...
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bioRxiv - Physiology 2024Quote: ... Sections were incubated with secondary antibody 1:1,000 Alexa fluor 488 goat anti Rabbit IgG (H+L) (Invitrogen, cat# A-11008) 1 hr at room temperature ...
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bioRxiv - Physiology 2024Quote: ... and incubated with secondary antibody 1:1,000 Alexa fluor 555 goat anti Rabbit IgG (H+L) (Life Technologies, cat#A-A21428), 1:1,000 Alexa fluor 568 goat anti Mouse IgG2b (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR reactions were carried out with 5 µL template volumes in a final reaction volume of 10 µL volumes in MicroAmp 96-Well Fast Reaction Plates (ThermoFisher, #4366932). Each target or multiplex combination was prepared with TaqPath 1-Step RT-qPCR Master Mix (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... substituted with 10% fetal calf serum (FCS; Capricorn Scientific, Palo Alto, CA, USA) and 2 mM L-glutamine (Invitrogen, Carlsbad, USA), 50 U/ml penicillin/streptomycin (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... in PBS + 3% NDS at RT for 1 h followed by Goat anti Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (DyLight 405, Invitrogen #PI35500) in PBS + 3% NDS at RT for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... and goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody conjugated with Alexa Fluor 546 (A-11030; Thermo Fisher Scientific). The following inhibitors or solutions were used for IVM or embryonic culture assays ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/) ...
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bioRxiv - Evolutionary Biology 2024Quote: Fluorescently labeled samples were mounted in glycerol mounting solution (80% glycerol, 0.1 M Tris, pH 8.0) on microscope slides coated with poly-L-lysine (Thermo Fisher Scientific #86010). Samples were imaged at ×40 on a Leica TCS SP8 confocal microscope ...
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bioRxiv - Immunology 2024Quote: ... slices were incubated with the following secondary antibodies in PBS-Triton 0.01%: F(ab’)-goat anti-rabbit IgG (H+L) A488 (Invitrogen, A-11070) 1:1000 ...
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bioRxiv - Immunology 2024Quote: Whole intact tumours were harvested on days 10-13 post engraftment and immediately immersed in fixative solution (1% PFA, 75 mM L-lysine [Sigma, Cat. L5501], 10 mM sodium m-periodate [Thermo Scientific Pierce ...
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bioRxiv - Bioengineering 2023Quote: ... 20 µL of 1:100 diluted fluorescent beads in DI water (FluoSpheres carboxylate-modified 0.2 µm, 660/680, F8807, Thermo Fisher Scientific), 6 µL 10% v/v tetramethylethylenediamine (TEMED ...
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bioRxiv - Cell Biology 2023Quote: ... followed by PBS-Tween washes and secondary antibody incubation (2h at room temperature): Horseradish peroxidase secondary antibodies: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen® ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed and then incubated with Horseradish peroxidase secondary antibodies against the last antibody: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen ® ...
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bioRxiv - Immunology 2023Quote: ... supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific), cultured for 4-7 days at 8% CO2 saturation and 37°C with shaking ...
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bioRxiv - Immunology 2023Quote: ... supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific), cultured for 4-7 days at 8% CO2 saturation and 37°C with shaking ...
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bioRxiv - Immunology 2023Quote: Antigen protein (100 µL; 10 µg/mL) in carbonic buffer (50 mM; pH 9.5) was immobilized on Maxisorp 96-well plates (Thermo Fisher Scientific) and incubated at 4 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with secondary antibody (Alexa Fluor 488 donkey anti-rabbit or Alexa Fluor 647 donkey conjugated to anti-goat immunoglobulin G (H+L) (Life Technologies). The coverslips were then mounted with DAPI (diamidino-2-phenylindole)-free Fluoro-Gel on glass slides (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... a 1.8 L preculture of BHK21 C13-2P cells (106 cells/ml) grown in Glasgow’s modified Eagle’s medium (GMEM; Thermo Fisher, MA, USA) supplemented with Bacto Tryptose Phosphate (1.5 g/L) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 25 µL of freshly made HiBiT reagent was added to new 96-well white opaque plates used to detect bioluminescence (Fisher Scientific). Corresponding wells in the growth plates were mixed well by pipetting up and down before transferring the same volume (25 µL ...
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bioRxiv - Genomics 2022Quote: ... Liver samples were collected from the organ and placed in 25 mL of RPMI-1640 media with 25 mM HEPES and L-glutamine (ThermoFisher Scientific) + 10% heat inactivated FBS (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were washed for 5 minutes 3 times with PBS followed by incubation with goat anti-Rat IgG (H+L) Alexa Fluor 594 secondary antibody (A11012, Thermo Fisher) at 1:500 dilution for one hour at room temperature ...
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bioRxiv - Immunology 2023Quote: Dried peptides were re-suspended in 50 µL Fe-NTA binding buffer (0.1% TFA, 80% acetonitrile; included in High-SelectTM Fe-NTA Phosphopeptide Enrichment Kit, Thermo Scientific #A32992).
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bioRxiv - Cell Biology 2023Quote: ... The secondary antibodies used in these experiments are goat anti-rabbit 488 and goat anti mouse H+L 568 (Life Technologies) at 1:1000 and goat anti guinea pig 568 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... neutrophils (2 × 106 cells per well) were seeded on poly-l-lysine coated coverslips in HBSS supplemented with 100 μM CellTracker™ Blue (Invitrogen) for 30min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... were detected with mouse anti-Opa 4B12 and mouse anti-porin H5.2 respectively followed by goat anti-Mouse IgG (H+L) Dylight 800 cross adsorbed secondary antibody (Invitrogen SA5-10176). All antibodies were diluted in blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... 100 μl of His-GLPR at 25 μM was incubated with 20x molar excess of EZ-Link NHS-PEG4-Biotin (Thermo Scientific) following supplier instructions ...
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bioRxiv - Microbiology 2023Quote: ... directed against the cytoplasmic domain of E-cad and revealed using an anti-mouse IgG (H+L) secondary antibody (Alexa Fluor 555) (Life Technologies). The 4’,6’-diamino-2-fenil-indol (DAPI ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were diluted at 5 million cells per 100μl in shearing buffer (1% SDS, 10mM EDTA, 50mM Tris-HCl pH8.0, 5mM NaF, Halt™ Protease Inhibitor Cocktail (Thermo Fisher), 1mM PMSF) ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated with goat anti-rabbit IgG (H+L) HRP conjugated secondary antibody (1:5000) (Thermofisher Scientific, 31460) for 1 hour at RT ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with plasmids expressing bRSV N-GFP and P in DMEM supplemented with 2% FCS.16 h post transfection medium was replaced with Leibovitz’s L-15 medium without phenol red (Gibco, ThermoFisher Scientific), and imaged live with a Leica TCS SP8 confocal microscope using a 63× oil immersion objective ...
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bioRxiv - Neuroscience 2023Quote: ... were maintained in DMEM with 1g/L of D-glucose supplemented with 8% Fetal Bovine Serum/FBS and 1% Pen/Strip (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: The expression of NPP-19 [1-301] fragment N-terminally fused to a 6xHis tag was induced by the addition of 1 mM of isopropyl β-D-thiogalactopyranoside (IPTG) to 1 L Luria Broth cultures of exponentially growing Escherichia coli BL21 DE3 pLysS (Invitrogen) strain (OD = 0.6) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were washed three times with PME buffer for 10 minutes on ice and mounted on glass slides in PME buffer plus 5 µg/L Hoechst 33342 (ThermoFisher Scientific) and squashed with a cover slip before sealing.
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bioRxiv - Neuroscience 2022Quote: ... RRID: AB_2893433), tdTomato (SICGEN, 1:500, RRID: AB_2722750) donkey anti-goat IgG (H+L) Alexa Fluor 488 (Invitrogen, 1:1000, A11057), Cy3 donkey anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... Following repeated washing in PBS slices were incubated in donkey anti-goat IgG H&L (Invitrogen Alexa Fluor 488; 1:500) and donkey anti-rabbit IgG H&L (Alexa Fluor 568 ...
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bioRxiv - Genetics 2023Quote: ... T1 Arabidopsis transgenic plants were selected on plates with MS medium containing 15 mg l−1 hygromycin B (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... E3.5 embryos were plated individually into gelatine-coated wells of 24-well plates in embryonic stem cell (ESC) medium (DMEM, high glucose 4500 mg/L (Gibco, ThermoFisher, #11965,), 100 µM 2-mercapto-ethanol (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... buffered to pH ranging from 7.2-7.5 with 2.2 g/L sodium bicarbonate and 15 mM HEPES (Gibco, Grand Island, New York). Media was supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK-293T cells for viral vectors production were cultured in DMEM high-glucose (w/ 4.5 g/l; #11995065; Thermo Fisher Scientific) with 10% heat inactivated FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were seeded onto poly-L-lysine (PLL)-coated 12-well plates in DMEM supplemented with 10% FBS and transfected using the RNAiMAX reagent (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: BP were dissected in cold L-15 media and incubated for 1 hour at room temperature with 5 µM pHrodo Red Intracellular pH Indicator (Invitrogen P35372) and 1 nM SiR-actin (SpiroChrome SC001 ...
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bioRxiv - Cell Biology 2023Quote: ... the basolateral medium was changed to the differentiation medium DMEM/ F-12 (1:1) supplemented with 2.5 mM L-glutamine (Fisher Scientific, Germany) + 2% Ultroser G (Cytogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Slides were washed three times in 1× PBS at RT and incubated in a combination of secondary antibodies (Cy3-conjugated goat anti-rabbit IgG (H+L) (Molecular Probes) and Alexa-488-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Biophysics 2023Quote: ... were plated in a 35-mm glass-bottomed dish coated with poly-L-lysine (Matsunami Glass Ind. Ltd., Osaka, Japan) and were incubated in Dulbecco’s modified Eagle medium (DMEM; Thermo Fisher Scientific) supplemented with 10 % fetal bovine serum (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... slides were washed with PBS and incubated for 2 h at room temperature with the secondary antibody (goat anti-rabbit IgG H+L conjugated with Alexa Fluor 488, Thermo Scientific), washed with PBS and mounted in VECTASHIELD® HardSet™ antifade medium with DAPI (Vector Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... MSCs were passaged twice a week in DMEM low glucose(1g/L)/pyruvate/HEPES medium (Gibco, ThermoFisher Scientific, Bleiswijk, The Netherlands) supplemented with 15% fetal calf serum (FCS ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... The total dissociated single cells were resuspended in 500 μL sorting buffer (25 mM HEPES, 5 mM EDTA, 1% BSA, in HBSS) containing Normal Rat Serum (1:100; Invitrogen, 10710C) and TruStain FcX™ (anti-mouse CD16/32 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µl of 5 µM targeting or non-targeting siRNAs were mixed with 190 µl reduced serum medium (Opti-MEM™, Gibco). In a separate tube ...