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Citations for Thermo Fisher :
8451 - 8500 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... containing TCEP (Pierce #77720) and were separated by SDS-PAGE on Bolt 4%–12% Bis-Tris Plus gels (Thermo Scientific #NW04122) and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2024Quote: ... 20-30 µg of total protein were loaded and separated using 7.5-10% SDS-PAGE gels then transferred onto PVDF membranes (ThermoFisher Scientific, #88518). Membranes were blocked using 15% skimmed milk in TBST at least for 3 hours at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The surface of the gel was then rendered permissive to cell adhesion by coating Rat Tail collagen I (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... The gel was run in 0.5x TBE buffer at 120 V for 42 min and stained with 1x SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen) diluted with 0.5x TBE buffer for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... The iBlot® Transfer Stacks were used to transfer proteins using the iBlot® Gel Transfer Device (Life technologies Corp. USA). We blocked the membrane with 3% P-Bovine Serum Albumin (P-BSA ...
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bioRxiv - Physiology 2024Quote: ... Twenty-five µg of protein were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Fisher Scientific). Western blot analysis was performed with the following antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... Equal protein amounts (25 µg) were resolved by SDS-PAGE on Bolt™ Bis-Tris Plus pre-cast 4-12% gradient gels (Invitrogen) and transferred to 0.45 mM polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... Efficacy of the process and final protein purity was assessed by SDS-PAGE using NuPAGE 4-12 % Bis-Tris Midi Protein Gels (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... The quality and concentration of DNA were assessed by agarose gel electrophoresis and the Qubit®3.0 Fluorometer (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... the integrity of total RNA was evaluated using agarose gel electrophoresis followed by reverse transcription with Maxima H Minus First-Strand cDNA Synthesis Kit (K1682; Thermo Scientific). Quantitative RT-PCR was performed with SYBR Green regent (Takara ...
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bioRxiv - Molecular Biology 2024Quote: ... extracts with high percentages of ssDNA were analyzed on denaturing gels (1 M urea, 0.8% agarose, 1x Tris-acetate-EDTA (TAE) buffer and 1x SybrGold (Thermo Fisher Scientific)) at 50 V for 3 hours ...
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bioRxiv - Microbiology 2024Quote: ... 2.5 µL of the reaction were then denatured in Laemmli buffer and run on a 8-16% polyacrylamide gel by SDS-PAGE and stained with either Brilliant Blue R250 or Coomassie Fluor Orange (Molecular Probes) and visualized on a Bio-Rad ChemiDoc MP imager.
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Microbiology 2024Quote: His-pulldowns and OD600-matched periplasmic extracts were mixed with LDS sample buffer and denatured at 95°C for 10 min before being separated on 4-12 % bis-tris gels in MOPS or MES buffer (Invitrogen, USA). Gels were then electroblotted onto a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2024Quote: ... Samples (20 ug/sample/lane) were loaded and run on a NuPage Bis-Tris 4-12% protein gel (Thermo Scientific, 23225), and transferred onto a 0.45 um PVDF membrane (Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Nicked plasmid pUC19 DNA was prepared by EcoRI digestion in the presence of 150 µg/ml EtBr77 and purified using the GeneJET Gel Extraction Kit (Thermofisher Scientific) to be used as the substrate for supercoiling assays ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 99 °C with shaking for 7 min and 25 μg of protein per sample was analysed with 4-12% Bis-Tris gels (Life Technologies) according to manufacturer’s instructions ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... and the RNA product purified from a 6% (vol vol-1) PAA-7M urea gel using a LowRange RNA ladder (ThermoFisher Scientific) for precise sizing ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of protein (40-50µg) were denatured and separated on 12% Bis-Tris SDS–polyacrylamide electrophoresis gels (NP0341BOX, Invitrogen, Carlsbad, CA) and transferred to a nitrocellulose membrane (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: Crude protein extraction obtained as described above was electrophoresed through a slab isoelectric focusing gel (pH 3-7, Invitrogen Novex EC66452) employing freshly made cathode and anode buffers (Novex) ...
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bioRxiv - Cancer Biology 2024Quote: ... 25-30 µg protein samples were run in SDS–PAGE followed by protein transfer using Mini Gel Tank and Mini Blot Module (Life Technologies). Immunoblotting was detected using near-infrared fluorescence (LI-COR ...
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bioRxiv - Biochemistry 2023Quote: ... vortexed and 5 μl was loaded onto a 15% pre-cast Novex Urea-TBE gel (Thermo Fisher Scientific Corp, Waltham MA) and run at 180V for 60 minutes alongside Small RNA Marker (Ab Nova GmbH ...
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bioRxiv - Cancer Biology 2023Quote: ... 5-20 μg of total protein was separated by NuPAGE 4-12% Bis-Tris Mini Protein Gels (NP0336BOX; Thermo Fisher Scientific) and transferred to Immobilon-P PVDF Membrane (IPVH00010 ...
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bioRxiv - Immunology 2023Quote: ... Equivalent amounts of total protein lysate were subjected to electrophoresis on NuPAG 4–12% Bis-Tris Protein Gels (Thermo Fisher Scientific) and proteins were transferred to polyvinylidene difluoride membranes (Millipore) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... of the homogenized protein-loading buffer sample were loaded in duplicates on a NuPAGE™ 3-8% Tris-Acetate gel (ThermoFisher) to be probed separately with FLAG and ATPase antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... either for 2-3 cm or ran completely and stained with colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). The lane was cut into bands ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... 10-20 µg of lysate or 25% of the immunoprecipitated samples were loaded onto NuPAGE 4–12% Bis–Tris Midi Gels (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RNA integrity was assessed by gel electrophoresis and its concentration quantified spectrophotometrically with a NanoDrop (Thermo Fisher Scientific, Waltham MA, USA). The obtained RNA was used to inoculate groups of 4-week-old N ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The quality of DNA was checked using 1.5% agarose gel electrophoresis and concentration was measured by Qubit 4 fluorometer with 1X dsDNA HS Assay Kit (Thermo Fisher Scientific). As the basis for ddRAD library protocol development ...
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bioRxiv - Genetics 2024Quote: ... Equal amounts of the tiled indexed PCR products were pooled together and the resulting pooled library of an expected size of ∼300bp was purified using a 4%EX e-gel (Life Technologies) followed by MinElute Gel Extraction (Qiagen) ...
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bioRxiv - Genetics 2024Quote: ... Samples were electrophoresed under a reducing condition on NuPAGE™ 4 to 12% Bis-Tris Mini Protein Gels (Thermo Fisher Scientific). Proteins on the gel were transferred to a Polyvinylidene Difluoride (PVDF ...
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bioRxiv - Evolutionary Biology 2024Quote: ... by recording the absorbance ratios at 260/280 and 260/230 followed by running a 0.7% agarose gel with SYBR safe staining (Thermo Fisher Scientific). Whole genome short read sequencing was undertaken by BGI Hong Kong ...
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bioRxiv - Genomics 2024Quote: ... The purified DNA solutions from the eight aliquots were combined and loaded into 5 lanes of 4% E-gel (Invitrogen, G401004). The DNA bands with the size between 300 bp and 1.2 Kb were excised ...
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bioRxiv - Genetics 2023Quote: ... Samples were heated at 70 °C for 10 minutes prior to loading and electrophoresis on a 10% Bis-Tris polyacrylamide gel (ThermoFisher, #NP0316BOX), followed by electroblotting on to a PVDF membrane ...
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bioRxiv - Microbiology 2024Quote: ... The quality and concentration of DNA were determined by 1.0% agarose gel electrophoresis and a NanoDrop2000 spectrophotometer (Thermo Scientific, United States). The hypervariable region V3–V4 of the bacterial 16S rRNA gene was amplified using the primers 338F (5’-ACTCCTACGGGAGGCAGCAG-3’)/ 806R (5’-GGACTACHVGGGTWTCTAAT-3’ ...
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bioRxiv - Immunology 2024Quote: ... The samples were run on BoltTM bis-tris plus 4-12 % gels using Bolt™ MES SDS running buffer (Thermo Fisher Scientific NW04122Box and B0002 ...
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bioRxiv - Biochemistry 2024Quote: ... 25% glycerol and 0.05% bromophenol blue] and were separated on 4 -16% Native PAGE Bis-Tris gradient gels (Thermo Fisher Scientific) (44) ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting PCR product was then purified using the Macherey-Nagel NucleoSpin gel and PCR clean-up kit (Thermo Fisher Scientific). Linearized vector (67.4ng ...
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bioRxiv - Cancer Biology 2024Quote: ... The conjugation efficiency was assessed by size shift of the conjugated antibodies on a 4-12% PAGE-gel (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... 30-60 μg protein samples were loaded into 4-12% Bis- Tris gels and transferred onto nitrocellulose membrane (ThermoFisher Scientific: 88018) using the BioRad Trans-blot Turbo System ...
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bioRxiv - Cell Biology 2024Quote: ... BN-PAGE was performed at 4°C on Native PAGE Novex 3-12% Bis-Tris Protein gels (Thermo Fisher Scientific, BN1001) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... cell extracts and purified protein samples were boiled at 95°C for 5 minutes followed by fractionation on NuPAGE Bis-Tris polyacrylamide gels (Thermo Scientific). Separated proteins were transferred to Immobilon FL polyvinylidene difluoride (PVDF ...
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bioRxiv - Zoology 2023Quote: ... The RNA quality was evaluated by gel electrophoresis and absorbance determination using a NanoDrop 2000 spectrophotometer (Thermo Scientific, New York, USA). Genomic DNA was removed ...
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bioRxiv - Molecular Biology 2024Quote: ... Following washes in RPE buffer RNA was eluted in RNase free water and analysed by electrophoresis on an agarose gel and quantified using the Qubit RNA High Sensitivity Assay Kit (ThermoFisher Q32852).
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bioRxiv - Biochemistry 2024Quote: Proteins (between 5 and 10µg) from tissue preparations were solubilized in Laemmli buffer before loading on top of a 4–12% NuPAGE gel (Life Technologies), stained with R-250 Coomassie blue (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... The Proemerald Q stain was performed following the manufacturer’s instructions (Pro-Q Emerald 300 lipopolysaccharide gel stain kit-Molecular Probes P20495). The gel was imaged using the Bio-Rad ChemiDocTM MP Imaging System.
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bioRxiv - Molecular Biology 2023Quote: DNA illustration by agarose gel electrophoresis has been conducted by exploiting 2% agarose gel and 1X TAE buffer (1 mM EDTA and 40 mM Tris-acetate, Fisher Scientific) and by applying constant voltage of 120 V for 40 to 60 min ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of total protein were mixed with 4X NuPAGE LDS sample buffer and 10X NuPAGE reducing agent and subsequently denatured for ten minutes at 95°C with a thermo mixer and proteins were separated by size using 4-12% Bis-Tris Plus Gels (Life Technologies) in a SDS Polyacrylamide Gel Electrophoresis System (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: Epimastigotes and trypomastigotes lysates containing 2 x 107 parasites in 20 µL of SDS-PAGE sample buffer containing 5 mM DTT were boiled for 5 min and loaded onto precast Novex Value 4-12% Tris-Glycine gels (Thermo Fisher). The electrophoresis was performed in 50 mM MOPS-50 mM Tris Base ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were heated at 70°C for 10 minutes and separated on 4-12% gradient Bis-Tris SDSPAGE gels (Invitrogen, NP0323) and transferred to 0.2μm nitrocellulose membrane (Bio-Rad Laboratories ...