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Citations for Thermo Fisher :
8451 - 8500 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... was dissolved in a diacetyl-L-tartaric anhydride(DATAN) solution (90 μl, 50 mG/ml in freshly mixed 80% acetonitrile/20% acetic acid, DATAN, Acros Organics). The solution was sonicated ...
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bioRxiv - Developmental Biology 2022Quote: ... Triplicate 20 µl reactions containing 1 µl of gDNA solution were amplified and denatured in the presence of MeltDoctor HRM Dye (Applied Biosystems) using an Applied Biosystems 7900HT Fast Real-Time PCR System according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... washed and equilibrated at 15°C for 30min in Hank’s balanced salt solution (HBSS) containing 1g/L glucose (Gibco, 14025-092) at a concentration of 500,000 cells/mL ...
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bioRxiv - Cell Biology 2022Quote: ... samples were diluted to 300 ng/µL (for the Orbitrap Elite) or 200 ng/µL (for the Q Exactive HF) in 5% HPLC grade acetonitrile (Fisher Scientific), 0.1% trifluoroacetic acid in deionized water ...
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bioRxiv - Cell Biology 2022Quote: ... the primary antibody was washed out and goat anti-mouse IgG (H+L) antibody conjugated with Alexa fluor 568 (Invitrogen; A11031) was added (1:1000 in PBS with Alexa Fluor 647 Phalloidin (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Translation activity at the midbody was assessed by detecting protein synthesis level using an L-HPG-translation kit (ThermoFisher, Cat# C10429) as previously described 44 ...
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bioRxiv - Genomics 2022Quote: ... SK-MEL-2 cells were obtained directly from the NCI-60 collection following written request and approval and were grown in RPMI-1640 media with 2 mM L-Glutamine (Gibco, 11875) with 10% FBS and 1X Pen/Strep ...
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bioRxiv - Genetics 2022Quote: ... Secondary antibodies: Goat anti-Mouse IgG (H+L) Superclonal Recombinant Secondary Antibody Alexa Fluor 555 (1:200, ThermoFisher Scientific catalogue #A28180), Goat Anti-Rabbit IgG H&L Alexa Fluor 488 (1:200 ...
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bioRxiv - Immunology 2022Quote: Human embryonic kidney HEK293T cell line was grown in DMEM medium containing 4.5 g/L glucose and sodium pyruvate (Gibco; 10569-010). Medium was supplemented with 10% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Genetics 2022Quote: ... 1×106 cells were plated per well in the prepared Matrigel plates in iN-NBM/B27 media {Neurobasal Medium without L-glutamine (Thermofisher 2110304) supplemented with 2% B27 (Thermofisher 17504044) ...
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bioRxiv - Developmental Biology 2022Quote: ... PV follicles were isolated from the ovary and incubated in 60% Leibovitz L-15 medium (Gibco, Thermo Fisher Scientific, Waltham, MA) in the presence or absence of recombinant goldfish activin B (5 U/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... or non-silencing control siRNA (Dharmacon, SMARTpool) at a final concentration of 10 nmol/L using Lipofectamine RNA iMAX transfection reagent (Invitrogen 13778150) in antibiotic-free growth medium ...
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bioRxiv - Immunology 2022Quote: ... The cells were transfected with 75 nmol/L siRNA targeting TLR2 (GenePharma, China) or siRNA negative control using 0.75 μl of Lipofectamin 3000 (ThermoFisher Scientific, USA). After 72 h ...
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bioRxiv - Microbiology 2022Quote: ... growth medium (200 µL, 24 h), bacterial cultures (200 µL, 24 h of growth)) and pre-cleaned type 1 glass vials (VOA; Thermo Scientific) were chilled on ice for 10 min ...
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bioRxiv - Immunology 2022Quote: ... and cells were collected in RPMI-1640 supplemented with 10% fetal bovine serum (HyClone) and antibiotics (Penicillin-Streptomycin-L-Glutamine, 100x (Gibco, Invitrogen)) ...
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bioRxiv - Molecular Biology 2022Quote: ... Dissociated hepatocytes were thereafter filtered through a 100 μm cell strainer and rinsed with ice-cold Leibovitz’s L-15 medium with GlutaMAX™ supplement (ThermoFisher Scientific). Hepatocytes were harvested at 100× g for 5 min at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 U Ribolock (40U/μl), 1x TaqMan RT primer per each gene of interest (miR-7a, miR-671, Let-7a, snoRNA202, U6 snRNA) (Applied Biosystems) and 1x first-strand synthesis buffer were filled up to 20 μl reaction volume with ddH2O and mixed ...
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bioRxiv - Microbiology 2022Quote: ... and resuspended to 106-107 CFU/ml in RPMI 1640 medium (with L-glutamine, buffered with MOPS; Thermo Fisher Scientific, USA). For Lactobacillus ...
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bioRxiv - Molecular Biology 2023Quote: ... TryPLE was then removed by twice washing the digested tissue in the dissection medium (Leibovitz’s L-15 Medium (Gibco, 11415-04) + 7 mM HEPES (Milian ...
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bioRxiv - Neuroscience 2023Quote: ... LDH assay was performed with 50 µl medium taken at 60 h post-transfection according to the manufacturer’s instructions (Pierce LDH Cytotoxicity Assay Kit, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... an overlay of DMEM (high glucose, +L-Glutamine) with 2% Hi-FBS and 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500) was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... a second agarose overlay containing DMEM (high glucose, +L-Glutamine) with 2% Hi-FBS 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500) and 200 ng/mL rifampicin was added 16 – 24 h after infection ...
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bioRxiv - Plant Biology 2023Quote: 0.5 μl of cDNA was mixed with appropriate qPCR primers (see Table S3) and Maxima SYBR Green qPCR Master Mix (Thermo Scientific). The qPCR reactions were run in technical triplicates on a Bio-Rad CFX ConnectTM thermal cycler ...
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bioRxiv - Plant Biology 2022Quote: ... For RT-PCR and RT-qPCR beads were washed in 20 μl 1 x SSIV buffer before resuspension in 12 μl sterile H2O with 1 μl dNTP 10 mM each mix and incubated for 5 min at 50 °C in a Proflex PCR machine (Applied Biosystems). Then the following were added 4 μl 10 x SSIV buffer ...
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bioRxiv - Physiology 2023Quote: ... Total liver cholesterol and triglycerides were determined with colorimetric assay kits (L-Type Triglyceride M – Fujifilm Wako, Infinity Cholesterol Liquid Stable Reagent – Thermo Fisher) and normalized to total protein content.
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bioRxiv - Microbiology 2022Quote: ... plates were washed three times with PBS-T and incubated with horse radish peroxidase (HRP)-conjugated goat anti-mouse IgG (H+L) (Thermo Fisher) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: Cell pellets were re-suspended in 240 µl PBS and 9 μl 10% triton X-100 containing 0.3 µl of 1 mg/ml Streptavidin conjugated with Alexa Fluor® 647 (Life technologies) and 0.6 μl of 1 mg/ml Wheat Germ Agglutinin (WGA ...
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bioRxiv - Cell Biology 2022Quote: ... The complex was diluted in 263 μl prewarmed imaging media (low glucose (1g/L) DMEM without phenol red (Thermo Fisher, #11054020) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... with 0.5% BSA and goat anti-rabbit IgG (H+L) horseradish peroxide (HRP)-conjugated secondary antibody (1:10,000, Thermo Fisher Scientific 31460) in TBST ...
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bioRxiv - Biochemistry 2022Quote: ... medium supplemented with 10 % fetal bovine serum (FBS) and 0.5 mM L-glutamine in humidified atmosphere at 37°C and 5 % CO2 (all from Gibco-Life Technologies).
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bioRxiv - Biophysics 2022Quote: ... DMT sample (4 μl) was applied to the chloroformtreated, negatively glow-discharged (10 mA, 15 s) grids inside the Vitrobot Mk IV (Thermo Fisher) chamber ...
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bioRxiv - Biophysics 2022Quote: ... Frogs are euthanized by immersion for 10 min in 1 L water + 5 g Tricaine-S (Pentair via Fisher Scientific #NC0342409) + 10 g sodium bicarbonate (Fisher Scientific # MK-7396-500) ...
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bioRxiv - Neuroscience 2024Quote: ... NPCs were cultured in 2% B-27-supplemented Neurobasal CTS Media (plus 2 uM L-glutamine and 0.2% vol/vol penicillin–streptomycin; Thermo Fisher Scientific) plus 20 ng/ml BDNF ...
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bioRxiv - Pathology 2024Quote: ... Myofibroblasts were grown on uncovered polystyrene culture plates in 16% FCS in DMEM supplemented with 1% Penicillin-Streptomycin-L-Glutamine and subcultured at a 1:3 ratio using Gibco TrypLETM Express Enzyme (12605010, Fisher Scientific) for cell dissociation ...
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bioRxiv - Cell Biology 2024Quote: ... and subsequently incubated for 30 min with secondary antibodies (Alexa Fluor goat anti-mousse IgG (H+L)/Alexa Fluor 647 secondary antibody (Invitrogen, A21236) at 1:1000 ...
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bioRxiv - Microbiology 2023Quote: Experiments were conducted in solution with an initial composition of either 8 g/l (0.14 molal (m)) NaCl solution (ThermoFisher Scientific, 207790010), 5.8 g/l (0.14 m ...
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bioRxiv - Neuroscience 2023Quote: ... NPCs to be terminally differentiated were passaged on three days after IL-6 stimulation (D21) into 50ng/ml poly-l-ornithine (Gibco; A3890401) and 20ng/ml laminin (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... and goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody (Alexa Fluor 546) (Cat. NO. A11003) were from Thermo Fisher Scientific.
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bioRxiv - Microbiology 2024Quote: ... The cells were washed three times with 200 µl PBS and resuspended in 40 µl of PBS/1% BSA containing 1/2500 of VybrantTM DyeCycleTM Violet stain (Invitrogen, V35003) to stain parasite DNA ...
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bioRxiv - Cell Biology 2024Quote: The hippocampus was dissected from TP18-day Sprague Dawley rat fetuses of either sex using cold dissection medium L-15 (Gibco, 21083027). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were next incubated in fluorescent secondary antibodies: AlexaFluor Plus 488 goat anti-mouse IgG (H + L) (1:500; A32723; ThermoFisher Scientific) and Texas Red Streptavidin conjugate (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/).
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bioRxiv - Cell Biology 2023Quote: ... and unlabeled L-ARGININE:HCL (final concentration 42.0 mg/mL) to RPMI SILAC growth medium [RPMI 1640 SILAC (Gibco, Thermo Fisher Scientific) supplemented with 100 U/ml penicillin (Gibco) ...
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bioRxiv - Physiology 2024Quote: ... The blocks were then treated with 1 mg/ml collagenase type II in Leibovitz’s L-15 Medium (Thermo Fisher Scientific Inc.) at 37°C for 30-45 minutes ...
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bioRxiv - Physiology 2024Quote: ... Sections were incubated with secondary antibody 1:1,000 Alexa fluor 488 goat anti Rabbit IgG (H+L) (Invitrogen, cat# A-11008) 1 hr at room temperature ...
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bioRxiv - Physiology 2024Quote: ... and incubated with secondary antibody 1:1,000 Alexa fluor 555 goat anti Rabbit IgG (H+L) (Life Technologies, cat#A-A21428), 1:1,000 Alexa fluor 568 goat anti Mouse IgG2b (H+L ...
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bioRxiv - Microbiology 2024Quote: ... 1 ml of the microbial cell suspension obtained was stained with 1 μL SYBR Green I (1:100 dilution in dimethylsulfoxide; shaded 20 min incubation at 37 °C; 10,000 concentrate, Thermo Fisher Scientific). The flow cytometry analysis of the bacterial cells present in the suspension was performed using a Cytoflex flow cytometer (CytoFLEX 3 ...
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bioRxiv - Molecular Biology 2024Quote: ... The mixture of SUMO protease cleavage products was dialyzed overnight (4 ºC, 4 L, 20 mM Tris-HCl, 150 mM NaCl, Slide-A-Lyzer→ cassette, 10 kDa, Thermo Scientific). The hexa-histidine tagged SUMO protein was separated from non-tagged authentic Mpro present in the dialysate by IMAC and collecting Mpro in the flow through ...
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bioRxiv - Neuroscience 2024Quote: ... in PBS + 3% NDS at RT for 1 h followed by Goat anti Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (DyLight 405, Invitrogen #PI35500) in PBS + 3% NDS at RT for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were then divided into two tubes of 540 μL sample to which was added 540 μL of phenol/chloroform/isoamyl alcohol (25:24:1, pH=7.9) (ThermoFisher, MA, USA). The samples were gently mixed by inverting the tubes and centrifuged at 13,100 g for 10 min ...