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Citations for Thermo Fisher :
8401 - 8450 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Samples were loaded onto a NuPage 4-12% Bis-Tris gel along with a combination of SeeBlue:Magic Marker (Thermo-Fisher Scientific, UK) 4:1 ratio (final volume 5 μl ...
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bioRxiv - Immunology 2022Quote: ... reactions were heated to 70 °C for 2 min and 15 μl loaded on 15% polyacrylamide gels containing 7 M urea (Invitrogen, EC8852BOX). Low range ssRNA Ladder (NEB ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the fragment of expected size was excised from the agarose gel and purified with the GeneJet PCR Purification kit (Thermo Scientific). For each sample ...
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bioRxiv - Genetics 2022Quote: ... The denatured samples were electrophoresed at 100-120 volts for 1.5-2.5 hours on NuPAGE 4-12% Bis-Tris Proteins Gels (ThermoFisher Scientific; catalogue # NP0321BOX) in NuPAGE MES SDS Running Buffer (ThermoFisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... The quality and quantity of the extracted RNA were assessed on 1.5% agarose gels and a NanoDrop Lite Spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Evolutionary Biology 2022Quote: ... was added to cDNA and the DNA was denatured at 95°C for 3 min prior to loading in Novex 6% TBE-urea gel (Thermo Fisher). Samples were separated for 45 min at 180 V ...
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bioRxiv - Immunology 2022Quote: ... heated at 95°C for 5min and separated on a Bolt 4-12% Bis-Tris gel (Cat. No. NW04122BOX, Thermo Fisher), alongside a Precision Plus Protein Dual Colour Standard (Cat ...
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bioRxiv - Microbiology 2022Quote: ... were loaded and separated on 8% and 12% SDS-PAGE gel and transferred onto a polyvinylidene difluoride transfer membrane (Thermo Fisher). Immunoblotting was performed by using the corresponding antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... The products were analyzed in a denaturing 6% polyacrylamide gel with a 5’-labeled RiboRuler Low Range RNA Ladder (Thermo Scientific) as size markers ...
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bioRxiv - Neuroscience 2022Quote: ... were loaded and separated electrophoretically using a 1.0 mm thick 4 to 12 % Bis-Tris gel together with NuPAGE MOPS SDS Running Buffer (both by Invitrogen™ NuPAGE). The gels were then electrotransferred onto nitrocellulose membranes (Hybond C Extra ...
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bioRxiv - Microbiology 2022Quote: ... The gel was run for 45 minutes at 100 Volts and RNA bands were visualized using SYBR Green II RNA Gel Stain (ThermoFisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... medium was removed and replaced with 2 mL of a standard agarose gel overlay prepared with 2X concentrated DMEM (Invitrogen #12100046) supplemented with 10% FBS and 1% NuSieve Agarose (Lonza ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA quality was checked on an agarose gel and genomic DNA contamination was removed using a DNA-free DNA removal kit (ThermoFisher; AM1906) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Digested DNA and PCR products were separated on 1 % agarose gels in Tris-borate-EDTA buffer and stained with SYBR™ Safe DNA stain (Invitrogen). For HRTV-DL ...
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bioRxiv - Microbiology 2022Quote: ... The quality and quantity of DNA was assessed by performing gel electrophoresis and the Qubit dsDNA BR Assay Kit (Thermo Scientific), respectively ...
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bioRxiv - Molecular Biology 2022Quote: ... at 100 V for 40 minutes using 1 μl of PCR product to 1 μl of 10x BlueJuiceTM Gel Loading Buffer (Invitrogen, UK). Gels were visualised with a Bio-Rad Gel Doc™ EZ Imaging System.
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bioRxiv - Microbiology 2022Quote: ... and heated at 70°C for 10 min before being loaded onto NuPAGE 4 to 12% bis-Tris protein gels (Life Technologies). The proteins were transferred using iBlot™ 2 Transfer Stacks (Life Technologies ...
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bioRxiv - Synthetic Biology 2022Quote: ... proteins were transferred from gels onto 0.45-μm Immobilon-P polyvinylidene difluoride membranes (PVDF) using a mini blot module (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins were denatured with NuPAGE LDS sample buffer and NuPAGE Sample Reducing Agent and then loaded on a precast 4-12% Bis-Tris gel (NuPAGE Novex, Life Technologies). After migration at 100 V ...
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bioRxiv - Plant Biology 2022Quote: ... The electrophoretic migration of biotin-labeled probes was resolved on 6% native polyacrylamide gels and detected using an enhanced chemiluminescence substrate (Thermo Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... RNA products were then excised from a 6% (vol vol−1) PAA-7M urea gel by comparison to a LowRange RNA ladder (ThermoFisher Scientific) and eluted overnight in elution buffer (0.1 M NaOAc ...
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bioRxiv - Microbiology 2023Quote: ... cells were transferred into a QIAshredder tube and mixed with 100 µl of gel loading buffer containing 2.5 ml of 4× LDS loading buffer (Thermo Fisher Scientific), 1 proteinase inhibitor tablet ...
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bioRxiv - Neuroscience 2023Quote: ... were subjected to SDS-polyacrylamide gel electrophoresis and transferred to PVDF membranes or nitrocellulose membranes and blocked with I-Block (ThermoFisher, T2015). Primary antibodies used were ...
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bioRxiv - Physiology 2022Quote: ... Proteins were denatured by 2-mercaptoethanol at 95°C and separated using SDS-PAGE in MOPS running buffer on a NuPAGE Bis-Tris 4-12% gel (ThermoFisher, UK). Separated proteins were transferred to nitrocellulose membranes (GE healthcare ...
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bioRxiv - Microbiology 2023Quote: ... The RNA quality and concentration was assessed by NanodropTM and visually on a 2 % E-Gel with SYBR safe (Invitrogen, Thermo) and visualized by E-Gel Power Snap Electrophoresis device (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... The samples and standards were then loaded onto a native PAGE 3 to 12% Bis-Tris Gel (BN1001BOX, Thermo Fisher Scientific), and electrophoresis was performed at 150 V for 3 hours on ice ...
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bioRxiv - Physiology 2022Quote: ... and loaded at 1.08 μg (Aug. 2021) or 1.3 μg (Aug. 2022) protein per well into a 4– 12% tris-glycine gel (Thermo Fisher Scientific). Electrophoresis was performed for 30 minutes at 60 V followed by an hour at 160 V ...
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bioRxiv - Biochemistry 2022Quote: ... Gels were run until the 37 kDa marker reached the bottom third of the gel and were immediately washed with water prior to incubation at room temperature in 1X renaturing buffer (Novex, ThermoFisher, LC2670) for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... DNA concentration and quality were checked via gel-electrophoresis and UV-Vis spectrophotometry (NanoDrop 1000c, Thermo Fisher Scientific, Waltham, MA, USA). DNA was stored at −20 °C until further processing.
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of protein (40-50µg) were denatured and separated on 12% Bis-Tris SDS–polyacrylamide electrophoresis gels (NP0341BOX, Invitrogen, Carlsbad, CA) and transferred to a nitrocellulose membrane (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... 25-30 µg protein samples were run in SDS–PAGE followed by protein transfer using Mini Gel Tank and Mini Blot Module (Life Technologies). Immunoblotting was detected using near-infrared fluorescence (LI-COR ...
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bioRxiv - Cancer Biology 2024Quote: Crude protein extraction obtained as described above was electrophoresed through a slab isoelectric focusing gel (pH 3-7, Invitrogen Novex EC66452) employing freshly made cathode and anode buffers (Novex) ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining sample was denatured at 95°C for 5 minutes in 4× Laemmli buffer and subjected to SDS-PAGE using 4-15% Mini-PROTEAN® TGX™ Precast Protein Gels followed by protein transfer to a 0.45-μm pore PVDF membrane (Cat#: 88585, ThermoFisher). Membranes were blocked in 3% BSA in TBS with 0.1% Tween-20 (TBS-T) ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with excess DTT was added to all reactions and samples were fixed for 5 min at 95°C before loading on a 4-12% Bis-Tris gel (Invitrogen WG1402BOX). The same samples without BS3 were run as a loading control and stained using a coomassie protein stain (Abcam ab119211 ...
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bioRxiv - Molecular Biology 2022Quote: IP samples were eluted with 40 µl 1xLDS buffer adjusted to 10 mM DTT (5 min, 90°C) and separated on 4-12% Bis-Tris NuPAGE gels (Thermo Fisher) followed by colloidal coomassie staining (Instant Blue ...
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: ... with 5% β-mercaptoethanol at 50°C for 10mins and loaded onto a 4-20% Tris-glycine SDS-PAGE gel (Invitrogen). The proteins were transferred to a nitrocellulose membrane with an iBlot (ThermoFischer) ...
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bioRxiv - Molecular Biology 2022Quote: ... the fragments were mixed in a 3:1 ratio with an equally digested and gel purified pJAK112 and ligated overnight at 4 °C using T4 DNA ligase (Thermo Scientific), resulting in pFF-170 (SpoY deletion ...
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bioRxiv - Molecular Biology 2022Quote: ... MF002 - plus-10) and the PCR products were fractionated on 8% TBE gel stained with SYBR Gold nucleic acid stain (Invitrogen, S11494). Quantitative PCR (qPCR ...
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bioRxiv - Biochemistry 2022Quote: Phagosomes or cells were lysed directly in 2x Laemmli buffer (with 5% ß-mercaptoethanol) and subjected to SDS-Page using 4-12% NuPAGE gels (Invitrogen) and immunoblotted to PVDF membrane ...
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bioRxiv - Plant Biology 2022Quote: ... DNA concentration was quantified using Qubit and the quality of DNA was visualized using 8% TBE gel (Invitrogen, Cat. No. EC62152BOX) performed at 230 V for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... LM/LAM samples were separated by SDS-PAGE (15% gel) and visualized using ProQ Emerald 488 glycan staining kit (Life Technologies). To detect LM/LAM in culture supernatants ...
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bioRxiv - Physiology 2022Quote: ... equivalent amounts of protein (40 μg) diluted in diH2O with laemmli buffer were denatured and separated on 4-12% Bis-Tris gels (Invitrogen; NW04120). Protein was then transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2022Quote: ... Aliquots of 30-40⍰μg of protein were loaded on a NuPAGE 4–12% denaturing Bis-Tris gel and transferred to a nitrocellulose membrane (Thermo Scientific). Membranes were incubated for 1h at room temperature in a blocking solution of 5% milk in TRIS Buffer Saline 0.05% Tween (TBS-T) ...
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bioRxiv - Immunology 2022Quote: BMDMs were lysed with RIPA buffer and the whole-cell protein lysates were separated on pre-cast Bolt 12% Bis-Tris Plus gels (Invitrogen, NW00127BOX) and transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2022Quote: ... the cells were collected into a QIAshredder tube and incubated for 1 min at RT with 100 μl of gel-loading buffer made from 2.5 ml of 4x LDS loading buffer (Thermo Fisher Scientific), 1 proteinase inhibitor tablet and 7.5 ml of PBS ...
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bioRxiv - Bioengineering 2022Quote: ... We loaded 200 μg RBC protein lysate into each well of a NuPAGE 4-12% Bis-Tris gel (Thermo Fisher Scientific) for protein separation by SDS-PAGE ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 μg of each protein lysates were separated by SDS-PAGE on 4-12% gradient gels (NuPAGE™, ThermoFisher Scientific, #NP0322) and transferred onto PVDF membrane (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 2019).The quality of co-expressed proteins were evaluated through resolving the protein bands on SDS-PAGE gel (Bolt 4-12%, Invitrogen™) Following the completion of protein expression ...
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.