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Citations for Thermo Fisher :
8401 - 8450 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Anaerobic conditions were achieved by incubating plates in an anaerobic jar with 3x AnaeroGen 2.5 L sachets (Thermo Scientific, UK, AN0025A) to generate anaerobic gas ...
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bioRxiv - Cell Biology 2019Quote: ... Conditioned medium was obtained by mixing 50% fresh DMEM supplemented with 10% FBS and 1% penicillin/streptomycin and 50% filtered medium obtained from U2OS cells grown for 48 h and supplemented with 1% L-glutamate (Life Technologies). After 10 to 14 days ...
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bioRxiv - Biochemistry 2019Quote: ... A 1 µL aliquot of trypsin (Pierce Scientific, MS-Grade; 1 µg/µl in 50 mmol/L acetic acid; Fisher Scientific) was added (final mass ratio of 1:50 trypsin:protein ...
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bioRxiv - Immunology 2020Quote: ... Human WT THP-1 and THP-1 KO cGAS cells48 were maintained in RPMI 1640 with L-glutamine and phenol red (Life Technologies) supplemented with 10% FBS ...
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bioRxiv - Genomics 2021Quote: ... donovani promastigotes of the strain MHOM/NP/03/BPK282/0 clone 4 (further called BPK282, reference genome of L. donovani) were maintained at 26°C on HOMEM medium (Gibco, ThermoFisher) supplemented with 20% Fetal Bovine Serum ...
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bioRxiv - Systems Biology 2020Quote: ... Single U-937 monocyte and macrophage cells were isolated and distributed into 1μl of pure water with MassPREP peptide mixture (25fmol/μl final concentration, Waters 186002337) in 384-well PCR plates (ThermoFisher AB1384) using a BD FACSAria I cell sorter ...
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bioRxiv - Cell Biology 2019Quote: ... Transformants with pPICZc plasmid were selected on yeast extract peptone dextrose (YPD) solid media containing 100 mg/l Zeocin (Gibco, #R25001). For transformation of cells with pIB2 or pIB4 plasmids ...
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bioRxiv - Biochemistry 2020Quote: ... and cells were washed twice with PBS and labeled with a secondary antibody (Alexa Fluor 488 donkey anti-Goat IgG (H+L) (A11055, 1:200, Life Technologies) in PBS for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... in TBST buffer with 0.5% milk and goat α-rabbit IgG (H+L) HRP conjugated secondary antibody (1:10,000, ThermoFisher Scientific 31460) in TBST were used to detect PstP-strep ...
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bioRxiv - Microbiology 2020Quote: ... BHK21 and Vero R cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) 4.5 g/L glucose (Gibco, Thermo Fisher Scientific Inc.) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... The antigens were then reacted with an Alexa Fluor 488-conjugated goat anti-mouse IgG H+L antibody (Thermo Fisher Scientific). The antibody titer was defined as the reciprocal of the highest dilution level at which a specific fluorescent signal was detected.
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bioRxiv - Cancer Biology 2020Quote: ... and HS27A (ATCC, VA) cells were maintained in RPMI-1640 with 2 mM L-glutamine and 25 mM HEPES (ThermoFisher, MA) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2021Quote: ... Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 488 (Thermo Fisher Scientific, A32731, 1:1000), PNA (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated in click reaction buffer (10 mM sodium-l-ascorbate, 20 μM biotin azide [Life Technologies], and 2 mM CuSO4) for 2h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were seeded on poly-L-lysine-coated (MW > 300,000, 0.1 mg/ml, Biochrom) 8-well chamber slides (Nunc Lab-Tek II) and transfected the next day (50-70% confluency) ...
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bioRxiv - Biochemistry 2021Quote: ... Dried pellets were resuspended in 43 µL of water and incubated with 5 µL of 10X TURBO DNase buffer and 2 µL of TURBO DNase (Thermo Fisher) for 1 hour at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Culture dishes (15cm) coated with poly-L-lysine were plated with 6×106 neurons each and cultures maintained in Neurobasal media (Thermo Fisher) supplemented with GlutaMAX (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were then washed with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin and treated with ACK lysis buffer (Thermo Fisher) for 5 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cat#PA1-8463) or goat-anti-monkey IgG (H+L, Cat#A18811) secondary antibody conjugated to horseradish peroxidase (HRP, Thermo Fisher) in blocking buffer at 1:10,000 or 1:4,000 dilution ...
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bioRxiv - Neuroscience 2020Quote: Adult C57BL/6J dissociated DRG cells were plated at a concentration of 5,000 – 10,000 cells / ml in tissue culture plates coated with poly-L-lysine (Invitrogen, 0.1 mg/ml) and laminin (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Australia) primary antibody and a donkey anti-mouse IgG (H+L) secondary antibody conjugated to Alexa Fluor 568 (A10037; ThermoFisher, France), essentially as described (28) ...
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bioRxiv - Cell Biology 2020Quote: ... and 1.25µl of gene specific primer (0.5µm from IDTDNA)] on a Step One Plus Real-Time PCR System (Applied Biosystems™, USA). PCR amplification was carried out at 95°C for 10s ...
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bioRxiv - Genetics 2021Quote: Circulating cells from ten larvae were isolated in 20 μL of 1X PBS for each biological replicate and total RNA was extracted using the PureLink™ RNA mini kit (Ambion) and quantitated using a spectrophotometer (Implen) ...
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bioRxiv - Biophysics 2021Quote: ... Sample aliquots of 100 μl were heated for 10 min over a temperature range of 20–80°C in a PCR thermocycler (Veriti, Applied Biosystems) and heat-denatured material pelleted at 18 000 × g for 30 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... After 2 washes cells were incubated for 1 hour with 0.5 μg/ml of Alexa Fluor 555 goat anti-mouse IgG (H+L) (ThermoFisher Scientific, #A21424). After 2 washes cells were resuspended in PBS and analyzed by flow cytometry using a FACS BD LSR-Fortessa.
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bioRxiv - Microbiology 2020Quote: ... we use 1µg/ml final concentration of ethidium bromide to stain parasitized erythrocytes and 4 µl/ml Alexa Fluor 488-conjugated goat anti-human IgG (H+L) (Invitrogen, Life Technologies), based on optimal signal to noise between positive control plasma (10 Mali highly exposed individual plasma ...
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bioRxiv - Genomics 2019Quote: ... a different group of divers sampled 1 L of seawater using clean Nalgene™ Wide-Mouth HDPE Bottles (Thermo Scientific™). After seawater collection ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were washed again as described and then incubated with secondary donkey anti-mouse IgG (H+L) Alexa Fluor 594 (Thermo Fisher Scientific Cat# A-21203 ...
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bioRxiv - Microbiology 2021Quote: ... aliquots of 50μl parasite cultures were washed in PBS and incubated 30 min with 1:1000 SYBR Green I DNA stain (Life Technologies). The fluorescence profiles of infected erythrocytes were measured on CytoFLEX (Beckman Coulter ...
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bioRxiv - Molecular Biology 2020Quote: RL-118 drug was diluted to a concentration of 20 μM (DMSO<1%) in tissue culture medium (DMEM with 10% FBS, 1% L-Glutamine, 1% penicillin-streptomycin, (Life Technologies)) ...
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bioRxiv - Immunology 2020Quote: ... a history of peanut-induced reactions consistent with immediate hypersensitivity and peanut-specific serum IgE levels greater than 5 kU/L (ImmunoCAP; Thermo Fisher). Subjects who met these criteria then underwent a double-blind placebo-controlled food challenge to confirm peanut allergy ...
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bioRxiv - Immunology 2021Quote: ... HTB-55) cells were maintained at 37°C in 5% CO2 in DMEM with high glucose and L-glutamine (Gibco 11965092), containing 10% heat inactivated fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: ... 10% fetal calf serum, 10 units/mL penicillin, 10 μg/mL streptomycin and 2 mM L-glutamine, all supplied by Gibco, ThermoFisher UK). To model interaction of naïve T cells and resting neutrophils ...
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bioRxiv - Microbiology 2021Quote: ... Alexa Fluor 647 (Jackson Immuno Research; Ref# 705-605-003) and donkey anti-rabbit IgG (H+L) Alexa Fluor 647 (Invitrogen; A32733). Flowcytometry was performed on a Becton Dickinson FACS Calibur and analyzed by Flow Jo.
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bioRxiv - Immunology 2020Quote: ... The secondary antibodies goat anti-rabbit IgG H&L (HRP) (GE NA934,1:4000) and HRP-labeled goat anti-human IgG (gamma chain) cross-adsorbed secondary antibody (Invitrogen, 62-8420) (1:1000) ...
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bioRxiv - Immunology 2021Quote: ... The vRNA was the eluted (45 μL) from which 5 μL was added in a 0.1 mL fast 96-well optical microtiter plate format (Thermo Fisher, CA) for a 20 μL RT-qPCR reaction ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times 1x PBS then incubated for one-hour room temperature with goat anti-rabbit IgG (H+L) Alexa Fluor Plus 488 (Invitrogen; A32731TR). After washing three times for 5 minutes with 1x PBS cells were fixed to glass microscopy slides (Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... cells were serum-starved with Mac media containing 1% serum for 3 hours before media replacement with Leibovitz’s (L-15) media (Invitrogen, Burlington, ON) supplemented with 1% serum ...
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bioRxiv - Genomics 2021Quote: ... mycoplasma tested) and HG002 cells (Coriell Institute, Camden, NJ; mycoplasma tested) were maintained in RPMI-1640 with L-glutamine (Gibco 11875093) supplemented with 15% Fetal Bovine Serum (VWR 89510-186 ...
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bioRxiv - Genetics 2020Quote: ... A Masterflex L/S peristaltic pump (Cole Parmer) was used for consistent dispensing of agar media in imagingtype monowell plates (Nunc #267060). A Caliper Sciclone ALH 3000 or Biotek MultiFlo FX instrument was used for liquid dispensing into microwell plates.
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bioRxiv - Cancer Biology 2021Quote: ... an additional 100 μL of EGM-2 without growth factor was added with 1.5 μL of 1 mg/mL calcein-am (ThermoFisher Cat # C1430). Cells were then imaged 30 minutes later at the 4-hour timepoint ...
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bioRxiv - Immunology 2022Quote: ... and 1 µl was injected into an UltiMate 3000 RSLC nanoflow system coupled to a Q Exactive HF-X mass spectrometer (Thermo Scientific). Peptides were reversed phase separated at a flow rate of 300 nL/min using a 25 cm Acclaim PepMap 100 C18 column (2 µm particle ...
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bioRxiv - Cancer Biology 2022Quote: ... PAR1-KO and PAR2-KO PC3 cells (100 µl per well) were plated in a 96-well cell culture plate (polystyrene, clear flat-bottom, Nunclon Delta, Nunc, ThermoFisher Scientific) at a cell density of 2 × 103 cells per well and cultured for 24 h in F-12K complete (10% FBS ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μL was injected with a split mode 1/20 at 260°C on a TG-5MS column (26098-1420, Thermo Scientific). Separation was performed in a Helium gas-stream at 1 mL.min−1 using the following temperature ramp ...
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bioRxiv - Molecular Biology 2022Quote: ... and subsequently transferred to a cell culture plate containing DMEM plus 10% FBS and antibiotics (penicillin 50 U/l and streptomycin 50 mg/ml; ThermoFisher Scientific). MaxSuppressor In Vivo RNA-LANCEr II (Bioo Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... and Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Alexa Fluor Plus 488 Secondary Antibody were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2022Quote: ... Amplified products were treated with 0.4U/μl Dpnl for 1 h at 37°C prior to the PCR purification with the GeneJET PCR Purification Kit (Thermo Scientific). 100 ng of DNA products were blunt-end ligated in 20 μl reactions with 40U/μl T4 DNA ligase in 1× T4 DNA ligase buffer overnight at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... and buffer exchanged into L-lactate (-) buffer (30 mM MOPS, 100 mM KCl, pH 7.2) using centrifugal spin column (10K MWCO, Thermo Fisher Scientific). Prior to analysis ...
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bioRxiv - Biophysics 2022Quote: ... The medium contains L-glutamine and sodium pyruvate and is supplemented with 10% (v/v) fetal bovine serum (FBS, ThermoFisher 10500064) and 1% penicillin-streptomycin (Gibco 15140122) ...
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bioRxiv - Biophysics 2022Quote: ... were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/F-12 mixture medium with L-glutamine and HEPES (1:1; 11330-032, Gibco, Thermo Fisher Scientific) at 37°C under 5% CO2 with 10% fetal bovine serum (FBS ...