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Citations for Thermo Fisher :
8351 - 8400 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 10μl of each LPS was mixed with 2x Novex™ Tris-Glycine SDS Sample Buffer and separated by gel electrophoresis for 1.5 hours at 30mA using Novex WedgeWell 12% Tris-Glycine Mini gels in Novex™ Tris-Glycine SDS Running Buffer (ThermoFisher). The gels were stained using sodium-m-periodate silver staining as described previously40.
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... Elution fractions were tested on 12% SDS-PAGE gel and fractions containing protein were pooled and dialyzed overnight at 4 °C using Snakeskin dialysis tubing 10K MWCO (Thermo Scientific) in kinase storage buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... DNA integrity was assessed using agarose gel electrophoresis and quantified with a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a Bio-Tek Powerwave HT microplate reader ...
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bioRxiv - Microbiology 2021Quote: ... the quality of genomic DNA was evaluated by pulsed-field gel electrophoresis (Sage Science Pippin Pulse) and genomic DNA concentration was quantified using a Qubit 3.0 Fluorometer (Life Technologies Q33216). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... 50 μl fractions were collected and analyzed by SDS–PAGE electrophoresis using 4–12% NuPage Bis-Tris precast gels (Life Technologies). Gels were stained with 0.01% (v/v ...
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bioRxiv - Immunology 2021Quote: The total amount of the pellets and 2.5 μg of input proteins were electrophoresed in Bolt 4–12% Bis-Tris Plus Gels (Thermo Fisher Scientific), and transferred onto polyvinylidene difluoride membranes with iBlot Dry Blotting System (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Precipitated proteins solubilized in 4X LDS Buffer with 2-mercaptoethanol were resolved by SDS-PAGE on Novex® Tris-Glycine Gels (Invitrogen) and transferred onto Immobilon-FL PVDF membranes (Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... SDS-PAGE was performed at 200 volts for 35 minutes following which the gels were stained with SimplyBlue Safe Stain (Thermo Fisher) to visualize the bands alongside a color prestained protein broad range standard (New England Biolabs).
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bioRxiv - Immunology 2020Quote: ... Equal amounts of protein (20-30 μg) were loaded on NuPAGE Bis-Tris 4%–12% gradient polyacrylamide gels in the MOPS or MES buffer system (ThermoFisher Scientific) and subsequently electrotransferred from mini gels to a solid nitrocellulose membrane using the iBlot 2 system (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... blood samples (60 to maximum 80 μL) were collected by retromandibular puncture in serum-separating tubes (Microvette 200 serum-gel, Sarstedt TPP, Fisher Scientific). After centrifugation ...
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bioRxiv - Immunology 2020Quote: ... The desired bands from the gel were excised and purified using GeneJET Gel Extraction and DNA Cleanup Micro Kit and ligated using T4 DNA Ligase (ThermoFisher, USA). After ligation ...
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bioRxiv - Immunology 2020Quote: ... The reduced polysaccharide was passed through two Bio-gel P2 mini columns and the level of biotinylation was detected with Pierce Biotin Quantitation Kit (28055, Thermo Scientific).
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bioRxiv - Immunology 2020Quote: ... Flash column chromatography was performed using silica gel (230-400 mesh, grade 60, particle size 40 to 63 μm) purchased from Fisher Scientific. pH 7 buffered silica gel was prepared by adding 10% weight pH 7 phosphate buffer to silica and rotating for ~12 hrs ...
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bioRxiv - Immunology 2020Quote: ... T7-Cas9 and T7-sgRNA PCR products were gel purified and used as the template for in vitro transcription with the MEGAshortscript T7 kit (Life Technologies) according to the kit protocol ...
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bioRxiv - Genomics 2020Quote: ... The quality and DNA concentration using both methodologies were verified in 1% agarose gel using the 1Kb Plus DNA Ladder (Invitrogen®) and also by Nanodrop DNA/RNA Quantification system.
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bioRxiv - Cell Biology 2021Quote: ... An equal amount of protein for each lysate was loaded into NuPAGE 10% Bis-Tris gels (Thermo Fisher Scientific, NP0301, US) (2017-2020) ...
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bioRxiv - Cell Biology 2020Quote: Protein lysates from induced (day 2) and non induced TAP110 overexpressing whole cells were separated on 10% NOVEX gradient SDS gel (Thermo Scientific) for 8 min at 180V in 1x MES buffer (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: For all other Western blot analyses 20μg protein lysates were loaded on 4–12% NuPAGE® Bis-Tris gels (Life Technologies) and later transferred to an Immobilon–FL membrane ...
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bioRxiv - Genomics 2020Quote: ... The quality and quantity of the isolated DNA were examined by 1% agarose gels (Figure S1) and a Qubit Fluorometer v2.0 (Thermo Scientific, USA) respectively ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were resolved on Bis-Tris SDS-PAGE gels in MOPS buffer (pH 7.7, 100-150 V) and transferred to nitrocellulose membrane (Thermo Fisher Scientific). Membranes were blocked for 1 h in 1x TBS containing 0.1% Tween20 (TBST ...
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bioRxiv - Cell Biology 2021Quote: ... 10 μg total protein per lane in Novex NuPAGE LDS-sample buffer supplemented with DTT were loaded on NuPAGE Novex 4-12 % bis-tris proteins gels in NuPAGE MES SDS running buffer (all: Thermo Scientific). Gels were blotted onto nitrocellulose membranes (Biorad ...
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bioRxiv - Genetics 2020Quote: ... PCR products were confirmed by agarose gel electrophoresis and sequentially either purified using the PureLink PCR Purification Kit (Thermo Fisher Scientific) or gel-extracted using the QIAquick® Gel Extraction Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins (30 micrograms per condition) were resolved on SDS-PAGE gradient gels and transferred to a polyvinylidene difluoride (PVDF) membrane (ThermoFisher Scientific). Blots were blocked in 5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were mixed withTM sample buffer and G250 loading dye and added to a 4-12% Bis-Tris NativePAGE gel (Life Technologies). BN-PAGE gels were run for 2 to 2.5 hours at 150 V using the NativePAGETM running buffer (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were separated by Bolt 4-12% Bis-Tris pre-cast gels and transferred onto nitrocellulose membranes using iBlot® transfer NC stacks with iBlot Dry Blotting system (Invitrogen). Membranes were blocked with buffer containing 0.05% v/v Tween-20 (Sigma P1379 ...
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bioRxiv - Immunology 2022Quote: ... Samples were heated in LDS (lithium dodecyl sulfate) loading buffer then loaded into 4–12% bis-tris gels (Thermo Scientific #NW04122BOX) followed by transfer to a PVDF membrane ...
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bioRxiv - Microbiology 2022Quote: ... These aliquots were stained with 1X Sybr Green I nucleic acid gel stain (Sybr Green I; Invitrogen by Thermo Fisher Scientific) for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... These aliquots were stained with 1X Sybr Green I nucleic acid gel stain (Sybr Green I; Invitrogen by Thermo Fisher Scientific) for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... All samples were stained with 1X Sybr Green I nucleic acid gel stain (Sybr Green I; Invitrogen by Thermo Fisher Scientific) for 15 minutes before acquisition on a flow cytometer ...
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bioRxiv - Microbiology 2022Quote: ... All samples were stained with 1X Sybr Green I nucleic acid gel stain (Sybr Green I; Invitrogen by Thermo Fisher Scientific) for 15 minutes before acquisition on a flow cytometer ...
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bioRxiv - Microbiology 2022Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific). For protein detection the following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Developmental Biology 2022Quote: ... Bands of interest between 150 and 500 bp were excised and purified using the GeneJET Gel Extraction Kit (Cat. #K0691, Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Then the gel was stained with Coomassie blue or silver staining according to manufacturer’s protocol (Pierce sliver stain kit, Thermo Fisher Scientific) to visualize the proteins.
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bioRxiv - Biochemistry 2022Quote: Peptides from on-gel digestion or on-beads digestion were analysed on a Orbitrap Fusion with UltiMate 3000 RSLC-nano System (Thermo Scientific). The raw data were searched using MaxQuant software (Version ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysate was loaded and resolved by SDS-PAGE on a 12% Bis-Acrylamide gel and electrophoresis was carried out at using NuPAGE MES SDS running buffer (ThermoFisher Scientific). The separated proteins were transferred onto a nitrocellulose membrane (BioRad Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: Total 40μg of protein from cell lysates were loaded in 8%SDS-PAGE gel and immunoblotted with RAD51 antibody (1:1000; PA5-27195; Thermofisher Scientific) diluted in 1%BSA+TBS+ 0.1%Tween 20 ...
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bioRxiv - Biochemistry 2022Quote: A Mettler-Toledo (Columbus, OH) Seven Easy model pH meter was used with a gel-filled pencil-thin pH electrode from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10 μg of extracted protein was separated using Novex SDS– polyacrylamide gel electrophoresis reagents and transferred to nitrocellulose membranes (Life Technologies). Membranes were blocked in 5% milk protein and incubated with primary antibodies overnight ...
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bioRxiv - Biochemistry 2022Quote: ... The gel was then washed 4 times for 5 minutes each with deionized water and stained with Imperial Blue stain (ThermoFisher #24615) for two hours before washing in deionized water overnight ...
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bioRxiv - Cancer Biology 2022Quote: Cell numbers were counted by digesting the 3D collagen gels (described in previous section) and counting the number of cells using Trypan Blue (Thermo Fisher) stain ...
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bioRxiv - Biophysics 2022Quote: ... The purified samples were also incubated in seven different buffers ranging in pH from 3.0 to 12.0 and then subjected to Blue Native PAGE using a NativePAGE™ Sample Prep Kit and a 3–12% Bis-Tris Gel (Invitrogen).
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bioRxiv - Cancer Biology 2022Quote: ... 15-20 μg protein samples were run in SDS–PAGE followed by protein transfer using Mini Gel Tank and Mini Blot Module (Life Technologies). Immunoblotting was detected using near-infrared fluorescence (LI-COR ...
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bioRxiv - Cancer Biology 2022Quote: ... and equal amounts of total protein were separated by NuPAGE 4-12% Bis-TRIS gel in MES SDS running buffer (ThermoFisher Scientific). Protein was transferred from the gel to a nitrocellulose membrane and blocked with Odyssey Blocking Buffer (LiCor ...
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bioRxiv - Biochemistry 2022Quote: ... PCR reaction mixture was loaded on 2.5% agarose gel (140 V for 2 h) and post-stained with SYBR gold (Thermo Fisher Scientific). Fractions between 100–400 nt were cut and DNA was extracted from the gel by Monarch DNA Gel extraction Kit (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... proteins contained in the eluates were subjected to SDS-PAGE analysis on a 4-12% gradient gel (NuPage, Thermo Fisher Scientific) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2022Quote: ... A total of 16 μg of proteins were loaded and separated on 4-12% polyacrylamide-SDS gels under reducing conditions (Invitrogen, NP0322) and transferred to nitrocellulose membranes (Amersham ...
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bioRxiv - Cell Biology 2022Quote: ... Gel was fixed with 40% methanol/10% acetic acid and then stained with colloidal Coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific) for 1 hour ...
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bioRxiv - Genomics 2022Quote: ... The lysate was run on a 10% SDS page gel with MOPS buffer and transferred using the iBlot system (ThermoFisher Scientific). The anti-Myc antibody (Abcam ...
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bioRxiv - Genomics 2022Quote: ... Bands of interest between 150 and 500 bp were cut out and purified using the GeneJET Gel Extraction Kit (Cat. #K0691, Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... All DNA samples were run in a 1% agarose 1X TBE gel and quantified using a Qubit fluorometer (Thermo Fisher Scientific) with a high-sensitivity dsDNA quantification kit (Invitrogen ...