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Citations for Thermo Fisher :
8351 - 8400 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The resulting cell lysate (L) was then supplemented with 2 mM GTP +/- 40 μM Taxol (Life technologies, cat. R0461 and P3456) and incubated at 37°C for 30 min to induce the polymerization of free tubulin into microtubules ...
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bioRxiv - Microbiology 2022Quote: ... two 10 µL aliquots of each dilution (technical replicates) were spread onto CDMN agar supplemented with erythromycin (100 mg/L, Acros Organics). Erythromycin supplementation further reduces growth of bacteria from mouse feces and has no impact on C ...
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bioRxiv - Pathology 2022Quote: ... confluent 12-well Vero plates were inoculated with 250 μL of 1:10 and 1:20 dilutions of macaque tissue homogenate in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) supplemented with 2% fetal bovine serum and allowed to absorb at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: Sample preparation for live-cell imaging: Transfected RPE-1 cells were imaged in phenol-free Leibovitz’s L-15 Medium (Gibco, 21083-027) at 37°C.
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was generated from the total RNA by using Maxima H Minus Reverse Transcriptase (200 U/L) according to manufacturer’s protocol (Thermo Fisher Scientific). Quantitative Real-time PCR (qPCR ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μl of this mix was dropped on a freshly plasma cleaned 5 mm round glass coverslips (Art. No. 11888372, Thermo Scientific). Coverslips were then transferred to a small humidified chamber and incubated at 37 °C until gel formation was complete ...
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bioRxiv - Cancer Biology 2022Quote: ... or siGRHL2 #2 (qPCR validation) (Dharmacon, #L-014515-02-0050) using 2.5 ug/mL of Lipofectamine 2000 (Thermo Fisher Scientific, #11668019) in Pen/Strep-free media.
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bioRxiv - Developmental Biology 2022Quote: ... PV follicles were isolated from the ovary and incubated in 60% Leibovitz L-15 medium (Gibco, Thermo Fisher Scientific, Waltham, MA) in the presence or absence of recombinant goldfish activin B (5 U/ml ...
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Purkinje cardiomyocytes of the ventricular conduction system are highly diploid but not regenerativebioRxiv - Developmental Biology 2022Quote: ... subsequently with goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody AlexaFluor 488 conjugate 1:1000 (A11001, Thermo Fisher Scientific) to define CMs ...
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bioRxiv - Immunology 2022Quote: ... (36) was propagated in L929 cells (mouse fibroblast cell line, ATCC, CCL-1) cultured in RPMI1640 with L-Glutamine (Gibco, 22400089) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Transverse cryosections were cut at 12-micron thickness and mounted onto poly-L-lysine pre-coated glass slides (Superfrost Plus; Thermo Fisher). For mCherry and ZPR2 staining ...
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bioRxiv - Pathology 2019Quote: ... After loading 100 μl of the sample-specific PCR mixture into one sample port of the microfluidic cards (Human ABC Transporter Panel; Applied Biosystems), the cards were centrifuged twice for 1 min at 280g and sealed to prevent well-to-well contamination ...
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bioRxiv - Immunology 2019Quote: ... Dead cells were discriminated using the LIVE/DEAD (L/D) fixable viability dye eFluor 506 or near-IR dead cell stain kit (Thermo Fisher) and samples were stained for extracellular and intracellular markers ...
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bioRxiv - Immunology 2020Quote: ... 10% fetal calf serum, 10 units/mL penicillin, 10 μg/mL streptomycin and 2 mM L-glutamine, all supplied by Gibco, ThermoFisher UK). Cytokines added were ...
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bioRxiv - Microbiology 2019Quote: ... 1 µL of cDNA was used as template in a 10 µL qRT-PCR reaction performed with Power SYBR reagent (Applied Biosystems). qRT-PCR was performed on an ABI QuantStudio 6 using the ∆∆Ct method ...
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bioRxiv - Molecular Biology 2019Quote: ... bakeri worm lysates or EVs were carried out with rat polyclonal anti-exWAGO antibody (raised against full length protein) or rat IgG (control) using protein L magnetic beads (Fisher Scientific). Equivalent amounts of the input ...
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bioRxiv - Genetics 2019Quote: ... The library was cleaned up each time using 2 μl of AMPure XP DNA beads per 1 μl of sample and we added 3 μl of RNase OUT (Thermo Scientific) to protect the RNA ...
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bioRxiv - Bioengineering 2019Quote: ... Mouse-anti-His6 primary antibody and goat anti-mouse IgG H&L (Alexa Fluor® 488) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Bioengineering 2019Quote: ... Neurons were plated on glass coverslips coated with 1 mg/mL poly-L-lysine at an approximate density of 70 cells/mm2 and were transfected with Lipofectamine 2000 (Life Technologies). All neurons used in this study were between days in vitro (DIV ...
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bioRxiv - Biochemistry 2019Quote: ... Secondary antibodies were donkey anti-mouse 800CW and goat anti-rabbit IgG (H+L) Alexa Fluor 680 (Li-COR Biosciences and Thermofisher, respectively). Immunoblots were analysed and protein densities quantified using an Odyssey CLx imager and Image Studio Lite software (Li-COR Biosciences).
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bioRxiv - Immunology 2020Quote: ... 10% fetal calf serum, 10 units/mL penicillin, 10 μg/mL streptomycin and 2 mM L-glutamine, all Gibco, ThermoFisher UK). Single cell suspensions were prepared by passing the cells through a 19G needle ...
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bioRxiv - Immunology 2020Quote: ... 10% fetal calf serum, 10 units/mL penicillin, 10 μg/mL streptomycin and 2 mM L-glutamine, all supplied by Gibco, ThermoFisher UK), with the correct combination of cytokines and neutralizing antibodies as follows ...
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bioRxiv - Cell Biology 2019Quote: ... 2X SSC and adhered to 0.01% Poly-L-Lysine coated coverslips before mounting in ProLong™ diamond antifade mountantwith DAPI (Life Technologies).
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bioRxiv - Systems Biology 2019Quote: ... 5μl of cDNA were used and amplification of selected target genes was detected using PowerUp SYBR Green Master Mix (Applied Biosystems) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Ligand was added in 0.4 μL of appropriate dilution in DMSO and 10 μL of DBC working solution was added using a Multidrop Combi (Thermo Scientific) and shaken for 10 seconds (final 5 μM) ...
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bioRxiv - Genomics 2020Quote: ... Hs578Bst was cultured with Hybri-Care Medium (ATCC, catalog#. 46-X) and supplemented with 1.5 g/L sodium bicarbonate (ThermoFisher, catalog# 25080094), 30 ng/ml mouse EGF (ThermoFisher ...
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bioRxiv - Synthetic Biology 2020Quote: ... 2 µL of 1.25 µM 6-FAM-labeled ADO-22 primer and 1 µl of 100 mM ddTTP (USB-Affymetrix, Cleveland, OH). Fluorescently-labeled cDNA fragments were analyzed by capillary electrophoresis (CE) ...
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bioRxiv - Developmental Biology 2020Quote: ... overnight at 4°C and further stained with 1:200 Alexa Fluor 633 Goat anti-Rabbit IgG (H+L) Secondary Antibody (Invitrogen A21071) at a 20°C for 3 hours ...
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bioRxiv - Biophysics 2019Quote: ... 4 µL of sample was blotted onto the grids and grids were blotted for 4 s using a Vitrobot IV (Thermo Fisher) before plunge freezing into liquid ethane ...
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bioRxiv - Cancer Biology 2020Quote: ... PI3K was then immunoprecipitated with 5 μl of antibody (anti-p85α, MilliporeSigma, Burlington, MA, USA) and 60 μl of Pierce Protein A/G magnetic beads (Thermo Scientific). PI3K activity in the immunoprecipitates was then assayed by PI3K ELISA according to the manufacturer's instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Acquisitions were performed in 1,000-10,000 m/z range with a 4 s scan time in positive mode The mass spectrometer was also calibrated using singly charged ions produced by a 2 g/L solution of cesium iodide (Acros organics, Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The mass spectrometer was calibrated using singly charged ions produced by a 2 g/L solution of cesium iodide (Acros organics, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... LS-C40832, rabbit polyclonal anti-RORC/RORC2 L-C116877 both from LSBio (Seattle, WA) and mouse mAb anti-c-Myc (ThermoFisher, 9E10).
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bioRxiv - Molecular Biology 2020Quote: ... RNA was reverse-transcribed by addition of 3 μl of RT mix (2 μl 5x SSIV buffer [Invitrogen], 0.5 μl 0.1 M DTT, 0.25 μl Superscript IV reverse transcriptase [Invitrogen], 0.25 μl of RNasin) and incubation for 5 min at 25°C ...
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bioRxiv - Neuroscience 2021Quote: ... We used the following secondary antibodies conjugated with Alexa Fluor fluorochromes: 488 goat anti-guinea-pig IgG H+L (catalog #A11073, Invitrogen/ThermoFisher), 555 donkey anti-rabbit IgGs H+L (#A-31572) ...
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bioRxiv - Microbiology 2021Quote: ... 1x106 - 1x107 cortical neurons were plated onto poly-L-lysine coated 15cm diameter culture dishes and later cultured in Neurobasal Media (Thermo Fisher) supplemented with GlutaMAX Supplement (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... K562 cells were maintained at 37°C with 5% CO2 in R10 media: RPMI with stabilized L-glutamine (Thermo Fisher 11875119) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2020Quote: ... The following secondary antibodies were used: Alexa Fluor 488 goat anti-mouse IgG (H+L) (A-11001, 1:500; Molecular Probes), Alexa Fluor 555 goat anti-rabbit IgG (H+L ...
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bioRxiv - Systems Biology 2021Quote: ... lyophilized peptides were resuspended in 105 µl of equilibration buffer (1% TFA/ 80% ACN) and the peptide concentration was measured optically at 280nm (Nanodrop 2000, Thermo Scientific) and subsequently equalized using equilibration buffer ...
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bioRxiv - Immunology 2020Quote: ... Unconjugated primary antibodies were detected with fluorescently labeled species appropriate secondary antibodies including Goat Anti-Rabbit IgG (H+L) Alexa Fluor 594 (ThermoFisher Scientific) and Goat Anti-Rat IgG (H+L)-Alexa Fluor 647 (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... EPS cells were pushed toward differentiation using TX media 70: TX medium formulation was DMEM/F12 without HEPES and L-glutamine (Life Technologies), 64 mg/l l-ascorbic acid-2-phosphate magnesium ...
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bioRxiv - Microbiology 2020Quote: ... chamber slides were incubated with secondary antibody Alexa-Fluor 488 goat anti-human IgG (H+L) (Invitrogen #A-11013, Paisley, UK) diluted 1:500 and Hoechst 33342 (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Infectious RNA (3 μg) was diluted in 100 μl of serum free L-15 media and mixed with Lipofectamine™ 3000 (Invitrogen) transfection reagents following the manufacturer’s instruction ...
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bioRxiv - Physiology 2020Quote: ... were collected from a local abattoir and transported on ice in 100 mL of transport medium [Leibovitz’s L-15 medium (Thermo Fisher, UK) supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... and Pierce Goat Anti-Mouse IgG (H+L) peroxidase conjugated goat secondary antibody (Cat # 31430, AB_228307) were purchased from Invitrogen (Waltham, MA). Cy™5 AffiniPure Donkey Anti-Rabbit IgG (H+L) ...
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bioRxiv - Bioengineering 2020Quote: ... and 1500 mg/L sodium bicarbonate supplemented with FBS (10%v/v) and 1X P/S (Thermo Fisher Scientific, Waltham, MA). B13-24 cells are capable of producing up to 100 μg/L of humanized immunoglobulin IgG4 at a rate of 25 pg/cell/day38.
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bioRxiv - Microbiology 2019Quote: ... and samples were resuspended in 40 μl of sterile ddH2O and sequenced at the University of Wisconsin-Madison Biotechnology Center using an ABI 377 instrument (Applied Biosystems).
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bioRxiv - Immunology 2020Quote: ... and 105.8 mg/mL L-Arginine U-13C6,U-15N2 R10 (CKGAS, #CNLM-539-H-0.25)) prepared in RPMI SILAC media (Thermo Scientific, #88365) supplemented with 10% dialyzed FBS (HyClone ...
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bioRxiv - Molecular Biology 2019Quote: ... washed twice with 80% ethanol and resuspended in 7 µL nuclease-free water before in vitro transcription at 37°C for 14 hr using the MEGAScript T7 kit (Invitrogen, AM1334). Library preparation was done as described in the CEL-seq protocol with minor adjustments 4 ...
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bioRxiv - Cancer Biology 2019Quote: ... or siRNA targeting ADK (GE Dharmacon L-062728-00-0005) were transfected at 12.5 nM using Lipofectamine RNAiMAX (ThermoFisher Scientific #13778150) according to manufacturer’s instructions.