Labshake search
Citations for Thermo Fisher :
8301 - 8350 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... EV lysates corresponding to 5–10 µg of total protein were run into 4-12% Bis-Tris plus gels (Invitrogen, ThermoFisher Scientific). Prior to loading ...
-
bioRxiv - Cell Biology 2021Quote: ... First dimensional BN-PAGE was run on NativePAGE™ 3-12% Bis-Tris gels under native conditions at 150V for 30 minutes with 1x native cathode buffer (Invitrogen) followed by 90 minutes at 250V in 0.1x native cathode buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... Prepared samples were run on NativePAGE™ 3-12% Bis-Tris gels under native conditions at 150V for 30 minutes with 1x native cathode buffer (Invitrogen) followed by 90 minutes at 250V in 0.1x native cathode buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... The integrity of each sample was checked by loading 5 μL of total RNA alongside a spike-in control (P4P62HP, 50 ng) onto a PAGE-Urea-TBE gel and visualized by SYBR Gold (Thermo Fisher). Subsequently ...
-
bioRxiv - Immunology 2020Quote: 1 μg of SARS-Cov-2 S protein was diluted in loading buffer and loaded on 10–20% Novex Tricine pre-cast gel (Invitrogen, USA). The run was performed at 120 V for 1 h ...
-
bioRxiv - Immunology 2020Quote: ... and heated at 70°C for 5 min before separation on 4-12% Bis-Tris Plus ready gel (Thermo Fisher Scientific). To determine PGE2R glycosylation in the hemolymph samples ...
-
bioRxiv - Immunology 2020Quote: Protein samples in reducing condition were mixed with loading buffer containing β-mercaptoethanol and heated at 96 °C for 10 minutes before loading on 4-12% Bis-Tris Plus gels (Invitrogen). Samples in non-reducing conditions were diluted in loading buffer without any other treatment ...
-
bioRxiv - Biochemistry 2019Quote: ... Pellet samples were resuspended in Laemmli buffer without bromophenol blue and electrophoresed in a 4520% acrylamide Tris-Glycine gradient gel (Life Technologies) for ~3 min ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... DNA quality and quantity were assessed using agarose gel electrophoresis and the Qubit Fluorometer with the dsDNA BR Assay (Life Technologies). ddRADseq libraries were prepared using a modified version of the Recknagel et al ...
-
bioRxiv - Microbiology 2019Quote: ... After extraction the DNA was checked for quality by gel electrophoresis and quantified using a Qubit 4 (Thermo Fisher Scientific, U.S.A.).
-
bioRxiv - Biochemistry 2019Quote: ... Lysates were mixed with 6X sample buffer containing β-ME and separated by SDS-PAGE using NuPAGE 4-12% Bis-Tris gels (Invitrogen), followed by transfer to 0.45μm Nitrocellulose membranes (GE Healthcare) ...
-
bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were subjected to SDS-PAGE using pre-cast 4-12% BOLT Bis-Tris Plus gels (ThermoFisher Scientific) and SDS Running MOPS buffer (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2020Quote: ... polyadenylated mRNAs were electrophoretically resolved in a 1% 1× TBE agarose gel between two RNA ladders (RiboRuler High Range, ThermoFisher Scientific) and analyzed using Image Lab™ Software 6.0.1 (Bio-Rad) ...
-
bioRxiv - Neuroscience 2019Quote: ... Samples were centrifuged at full speed for 3 min before loading on NuPage 4%–12% Bis-Tris Gels (Invitrogen, Carlsbad, CA). Gels were run at 140V for 55 min ...
-
bioRxiv - Systems Biology 2019Quote: ... Equivalent amounts of protein in the specimen were separated based on their molecular weight by electrophoresis on 8% Bis-Tris gels and transferred to polyvinylidene difluoride (PVDF) membranes (Life Technologies). Membranes were immediately blocked with 5% nonfat dried milk diluted in TBST and incubated with primary antibodies (1:1,000 ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... DNA concentration was evaluated through agarose gel electrophoresis and fluorometry using a Qubit 2.0 (Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA). Library preparation ...
-
bioRxiv - Cell Biology 2019Quote: ... were poured into their respective tank compartment and the appropriate amount of protein (55μg or 5×105 parasites) was loaded per lane on a NativePAGE™ 4-16% Bis-Tris Gel (Novex-Life technologies). 5 μl NativeMark™ (Invitrogen ...
-
bioRxiv - Genomics 2019Quote: About 30 μg of total RNA isolated with Trizol was loaded into each lane of agarose gel and blotted onto membrane with Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher) according to manufacturer’s instruction ...
-
bioRxiv - Genomics 2021Quote: ... Purified DNA was eluted with 4 μL of 10 mM Tris-acetate (pH 8.0) and analyzed on a 10% Novex TBE-Urea gel (Thermo Fisher Scientific). Fluorescent images were obtained using Typhoon Trio+ and analyzed with ImageQuant software (GE Healthcare).
-
bioRxiv - Genetics 2020Quote: ... boiled in reducing sample buffer for 5 min and resolved on 4–12% Bis-Tris Bolt gels and transferred using an iBlot 2 (Thermo Fisher). Blots were blocked in 2.5% milk in 1% TBS-Tween before staining with antibodies (Table S5) ...
-
bioRxiv - Genetics 2020Quote: ... Amplicons obtained from the second PCR were purified by gel extraction and quantified using the Qubit dsDNA HS assay kit (Invitrogen #Q32851). Single-end 75 bp sequencing was performed on the NextSeq 500 platform by the Molecular Biology Core Facilities at Dana-Farber Cancer Institute ...
-
bioRxiv - Microbiology 2021Quote: ... Triplicate reactions were pooled after PCR and amplified DNA was visualized by agarose gel electrophoresis and quantified by Pico green (Thermo Fisher). After cleaning with Ampure beads (Beckman Coulter) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... A small aliquot of the Isolated DNA was run on a 1% agarose gel with a 1 kb plus DNA Ladder (Thermo Scientific) to check for degradation ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... RNA concentration was measured through electrophoresis by loading 1μl of each sample on 1% agarose gel and by spectrophotometry through Nanodrop 3300 (Thermo Fisher Scientific).
-
bioRxiv - Neuroscience 2021Quote: ... Equal amounts of protein (20-50 μg) were loaded and were separated by electrophoresis in 4 to 12% Bis-Tris Gel (ThermoFisher Scientific), transferred to polyvinylidene difluoride membranes ...
-
bioRxiv - Biochemistry 2020Quote: ... 500 mM 6-aminohexanoic acid) was added to lysates right before loading samples into a NativePAGE Bis-Tris gel 4-16% (Life Technologies). The proteins were separated according to Wittig et al.32 and transferred onto a PVDF membrane using a standard Tris-glycine transfer buffer with 0.05% SDS ...
-
bioRxiv - Molecular Biology 2021Quote: ... After gel isolation and cleaning inserts with Gel purification kit (Macherey Nagel) and destination vectors were ligated by using T4 DNA ligase (Thermo Scientific).
-
bioRxiv - Neuroscience 2021Quote: ... they were boiled for 10 min at 85°C before being loaded and separated in SDS–polyacrylamide gels 4–12% (NuPAGE Bis-Tris, ThermoScientific) in MOPS 1X buffer (NP0001-02, NuPAGE, Novex, Life Technologies). After migration ...
-
bioRxiv - Plant Biology 2021Quote: ... RNA quality was examined on a 1% agarose gel and RNA concentration was quantified using the Qubit RNA HS assay kit (Invitrogen, USA). 2ug of total RNA was used to construct stranded RNA-seq libraries using the Illumina TruSeq stranded total RNA LT sample prep kit (RS-122-2401 and RS-122-2402) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples were passed through a 25G syringe five times to reduce viscosity and separated on a 3%-8% NuPAGE Tris-Acetate gel (Thermo Fisher). Proteins were transferred onto a polyvinylidene fluoride membrane (Thermo Scientific ...
-
bioRxiv - Cell Biology 2019Quote: ... sonicated (30 s ON/30 s OFF for 5 min) resolved on 10.5% acrylamide/bis-acrylamide SDS–PAGE gels and transferred to nitrocellulose membranes (Thermo Fisher Scientific) in transfer buffer ...
-
bioRxiv - Developmental Biology 2021Quote: ... Proteins were quantified by the BCA assay (Pierce) and equal amounts loaded onto 4-12% Bolt Bis-Tris plus SDS-PAGE gels (Thermo Fisher) to separate proteins ...
-
bioRxiv - Biochemistry 2021Quote: ... and insoluble samples and were denatured in reducing Laemmli buffer at 100°C for 5-10 min prior to loading onto a 4-20%Tris-Gly SDS-PAGE gel (Life Technologies). Proteins were then transferred to a nitrocellulose membrane using an iBlot2 (Life Technologies) ...
-
bioRxiv - Biochemistry 2021Quote: ... x 20 000g and 15 μL of supernatant was loaded on SDS-PAGE gel (NuPAGE™ 4 to 12%, Bis-Tris, Invitrogen). Proteins were electrophoretically separated for 35 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Gel lanes were sliced into of 1-2 mm2 pieces and following in-gel trypsin digestion followed by liquid chromatography tandem mass spectrometry (LC-MS/MS) using an Orbitrap Q Exactive (ThermoFisher Scientific) mass spectrometer coupled to a nano-ultra performance liquid chromatography (UPLC ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were then loaded onto a 4-12% Bis-Tris SDS polyacrylamide gel which was then stained with Imperial Protein Stain (ThermoFisher Scientific). Gel lanes were sliced into of 1-2 mm2 pieces and following in-gel trypsin digestion followed by liquid chromatography tandem mass spectrometry (LC-MS/MS ...
-
bioRxiv - Molecular Biology 2021Quote: ... and size selection for the desired 200-800 bp DNA library fragments was performed using PureLink DNA gel extraction kit (Invitrogen, K210012). Library quality was confirmed by Agilent 2200 TapeStation Nucleic Acids System.
-
bioRxiv - Molecular Biology 2021Quote: Proteins were separated by SDS-PAGE on 4%–20% polyacrylamide gradient gels and transferred to 0.45-μm nitrocellulose membranes (iBlot2, PVDF, mini Transfer Stacks, Thermo Scientific; IB24002v). The membranes were incubated with the appropriate primary and secondary antibodies and washed with PBS-Tween 20 ...
-
bioRxiv - Molecular Biology 2021Quote: ... and size selection for the desired 200-600 bp DNA library fragments was performed using PureLink DNA gel extraction kit (Invitrogen, K210012). Library quality was confirmed using the Agilent 2200 TapeStation Nucleic Acids System (Agilent).
-
bioRxiv - Biochemistry 2021Quote: ... The bottom of the PAGE gel on which the chromatin samples were resolved is cut off and stained with Colloidal Coomassie (SimplyBlue, Life Technologies) to stain histones which provide loading controls and indications of sample contamination with egg extract (cytoplasm).
-
bioRxiv - Biochemistry 2021Quote: ... Samples were stained for DNA using either SYBR Green I Nucleic acid gel stain Ex504/Em523 (10,000 X concentrate in DMSO – subsequently diluted to 1:1000) (Invitrogen, Thermo Fisher, UK) or SYTO 9 Green Fluorescent Nucleic acid stain Ex485/Em498 ...
-
bioRxiv - Developmental Biology 2021Quote: The T7-sgRNA PCR product was gel-purified and employed as the template for IVT using the MEGAshortscript T7 kit (Life Technologies). Both the Cas9 mRNA and Peg10-ORF2-sgRNA were DNase-treated to eliminate template DNA ...
-
bioRxiv - Developmental Biology 2021Quote: ... The T7-Cas9 PCR product was gel purified and used as the template for in vitro transcription (IVT) using the mMESSAGE mMACHINE T7 ULTRA kit (Life Technologies). The T7 promoter was added to the Peg10-ORF2-sgRNA region of the pX330 plasmid by PCR purification using the primers listed below.
-
bioRxiv - Cancer Biology 2021Quote: ... Samples were then analysed by SDS-PAGE on 8% polyacrylamide gels using a PageRuler™ Prestained Protein Ladder (Thermo Scientific, 26616). Proteins where then transferred onto a nitrocellulose membrane (GE Healthcare ...
-
bioRxiv - Synthetic Biology 2021Quote: ... All assembled plasmids (pFB66 to pFB72, pFB96 to pFB130) were checked correctly by agarose gel electrophoresis after FastDigest XbaI (Thermo Scientific) digestion ...
-
bioRxiv - Genomics 2020Quote: ... An estimated 60 µg of protein was loaded per well on a NuPAGE Novex 3-8 % Tris-Acetate Protein gel (Life Technologies), separated by electrophoresis and blotted on polyvinylidene fluoride membranes ...
-
bioRxiv - Neuroscience 2020Quote: ... heated at 95 °C for 5 min and were run in on 8–16% NuPAGE SDS-PAGE gradient gel (Life Technologies) in MOPS SDS Running Buffer (Life Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were loaded onto 15% SDS-PAGE gels and run via standard methods in NuPAGE MOPS SDS running buffer (Invitrogen #NP0001). Gels were transferred (Transfer Buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... was denatured for 10 minutes at 90°C and loaded onto a Bolt BT Plus 4-12% gel (Invitrogen, Carlsbad, CA). After electrophoresis ...
-
bioRxiv - Microbiology 2020Quote: ... The extended products were purified by a NucleoSpin Gel and PCR Clean-up Kit and then analyzed using an ABI 3730xl DNA analyzer (Applied Biosystems) at Macrogen.