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Citations for Thermo Fisher :
8301 - 8350 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Secondary antibodies HRP-conjugated goat anti-rabbit IgG F(ab’)2 and HRP-conjugated anti-mouse IgG(H+L) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... falciparum strain 3D7 parasites were grown under 5% O2 and 5% CO2 in RPMI-1640 media supplemented with 5 g/L Albumax II (Life Technologies), 2 g/L sodium bicarbonate ...
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bioRxiv - Microbiology 2020Quote: ... 30,400 cortical neurons were added to poly-L-lysine coated glass coverslips in 24-well plates and later cultured in Neurobasal Media (Thermo Fisher) supplemented with GlutaMAX Supplement (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... transferred to a 10 cm dish and cultured from day 13 until day 25 in differentiation media without vitamin A (∼1 L differentiation media containing 500 ml DMEM/F-12 and 500 ml neurobasal media (Invitrogen, 21103049), 5 ml N2 supplement ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-associated or immunoprecipitated complexes were eluted (30 μl) at pH 2 for 5 min at 25°C and neutralized according to the manufacturer’s instructions (88805; Thermo Fisher Scientific). Samples were then reduced at 70°C for 5 min by dilution in 5x sample buffer (125 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... with 5 ng/μL ng of cDNA in each technical duplicate and the cycling parameters according to the protocol on a ViiA7 (Thermo Fisher). Primer and probe set assay IDs for the TaqMan assays were Hs00961640_g1 for SF3B1 and Hs03929097 for GAPDH ...
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bioRxiv - Neuroscience 2020Quote: ... with the appropriate secondary antibodies: Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 488 (Invitrogen, A23766) and Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... with 0.1% Tween-20 in 1X PBS pH 7.4 and then incubated overnight at 4°C with the appropriate secondary antibodies: Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 555 (Invitrogen, A32816) and Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... 14 mM Glucose and 4 mM L-AlanylGlutamine supplemented with 8% heat-inactivated fetal bovine serum (FBS) (#10082139; Thermo Fisher Scientific), EGF 5 ng/ml (#01-107 Millipore ...
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bioRxiv - Cancer Biology 2021Quote: OE33 cells were described previously (Grandal et al., 2017) and cultured in RPMI supplemented with 10% FBS and 2 mM L-glutamine (Thermo Fisher). Snu5 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed 3 times for 5 min and incubated for 30 min with secondary antibodies diluted in PBT + 0.5% BSA: goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 568 (1/500, Invitrogen #A-11031), goat anti-Rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2022Quote: ... CAT-1 (encoded by SLC7A1) and CAT-3 (encoded by SLC7A3) had 4 substrates including L-Lysine hydrochloride (Fisher Scientific #BP386), L-Histidine (Sigma-Aldrich #H6034) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: 10 μL aliquots of sample extracts were analyzed using liquid chromatography coupled to Orbitrap-based MS analysis (Dionex Ultimate 3000, Thermo Scientific Fusion or Thermo Scientific High-Field Q-Exactive) ...
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bioRxiv - Cancer Biology 2022Quote: ... and HCC4006 (ATCC) were cultured in RPMI 1640 with L-glutamine (Mediatech) supplemented with 10% heat-inactivated FBS (Thermo Fisher Scientific), penicillin (100 U/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions were plated onto poly-L-lysine (PLL)-coated plates in SVZ explant medium consisting of DMEM/F12 (Invitrogen, 11320074), 3% FBS (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were re-suspended at a concentration of 1 × 105 cells/μl in transplantation media (Leibovitz’s L-15 (Gibco, 11415-064), supplemented with caspase inhibitor (Promega G7231) ...
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bioRxiv - Immunology 2022Quote: ... Cells were further permeabilized with 0.2% Triton X-100 and incubated with cross-reactive rabbit sera anti-SARS-CoV-2-N for 1 hour at RT before adding an Alexa Fluor 488 goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (Life Technologies). The fluorescence density of SARS-CoV-2 infected cells were scanned using a Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... Lyophilized global and phosphorylated peptides were reconstituted in 12 μL of 0.1% FA with 2% ACN and 5 μL of the resulting sample was analyzed by LC-MS/MS using an Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo Scientific) connected to a nanoACQUITY UPLC system (Waters Corp. ...
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bioRxiv - Developmental Biology 2022Quote: ... the specimens were incubated for 1 hr at RT with anti-rabbit IgG(H+L)-Alexa 568-conjugated antibody (Donkey; Invitrogen, A10042), anti-mouse IgG2a-Alexa 488-conjugated antibody (Goat ...
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bioRxiv - Biochemistry 2022Quote: ... cells were incubated with secondary antibodies: Alexa Fluor 488® F(ab’)2 fragment of goat anti-mouse IgG (H+L) (Invitrogen) and/or Alexa Fluor 594® F(ab’)2 fragment of goat anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2022Quote: ... Cleaned library pools were adjusted to a concentration of 1.1 ng/µl ± 0.1 ng/µl and their concentrations were confirmed using the Qubit dsDNA high-sensitivity assay on a Qubit 3.0 fluorimeter (Invitrogen, Carlsbad, California). Microbial community sequencing on an Illumina MiSeq using 2 × 250 bp libraries (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... the beads were separated from the lysate and washed four times with 1 ml of CoIP buffer before resuspending in 15 μl of 1 x loading buffer (NuPAGE LDS sample buffer (4 x) and NuPAGE sample reducing agent (10 x) (ThermoFisher Scientific) diluted to 1 x with H2O) ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were then washed with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin and treated with ACK lysis buffer (Thermo Fisher) for 5 minutes ...
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bioRxiv - Physiology 2022Quote: ... Success of the transformation and choice of the recombinant plasmids was verified in LB agar cultures (Lennox L Agar, Thermo Fisher) containing Ampicillin (200 mg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... The secondary antibody was Alexa Fluor 549-conjugated goat anti-mouse IgG (H+L) (diluted 1:250 in PBST containing 5% goat serum, Invitrogen, USA) which was incubated overnight ...
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bioRxiv - Genomics 2022Quote: ... the nuclei were resuspended in 100-400 µl ST buffer without RNAse inhibitor and filtered with a 40 µm mesh filter attached to a FACS tube (Fisher Scientific). The nuclei concentration and dissociation quality was then determined in a 5 µl aliquot using Neubauer counting chambers (Bulldog Bio ...
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bioRxiv - Microbiology 2022Quote: ... injecting 2 µL in an analytical column (10 cm, PepMap RSLC C18, 2 µm, 100 A, 50 µm x 15 cm, Thermo Scientific). For chromatographic separation ...
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Understanding molecular mechanisms and predicting phenotypic effects of pathogenic tubulin mutationsbioRxiv - Genetics 2022Quote: ... cells were incubated with 20-50 nM SiR-tubulin dye (SpiroChrome) for 1-2 hours and transferred to L-15 medium (ThermoFisher Scientific) before imaging ...
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bioRxiv - Cancer Biology 2022Quote: ... AML cells were grown in RPMI L-Glutamine (4 mM) supplemented or minimum essential medium (MEM)-α Glutamax supplemented with 10% foetal bovine serum (FBS, Invitrogen), 100 IU/mL penicillin and 100 µg/mL streptomycin (Life Technology ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified T cells were labeled with far-red at a final concentration of 1 mol/L according to the manufacturer’s instruction (Molecular Probes, C34564). Far Red-labeled T cells were cultured similarly to above MLR for allogeneic reaction or Dynabeads T cell activator CD3/CD28 stimulation for up to 7 days ...
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bioRxiv - Immunology 2022Quote: ... the supernatants were removed and cell pellets were ressuspended in 400 µL of cold PBS-Glucose (1g/L) containing Sytox red 1/1000 (Catalog number: S34859, ThermoFisher scientific). The fluorescence of DHR123 were measured via Facscalibur flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... malayi parasites were washed several times in worm culture media warmed to 37°C (RPMI with 1% HEPES, 1% L-glutamine, 0.2% Penicillin/Streptomycin, and 1% w/v glucose (Thermo Fisher Scientific)) ...
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bioRxiv - Cell Biology 2022Quote: ... 3.7 g/l sodium bicarbonate and sodium pyruvate) (SH30243.01, Hyclone GE) supplemented with 10% FBS (10270-106, Gibco-Thermo Fisher Scientific) and 1% penicillin-streptomycin (A001A ...
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bioRxiv - Cell Biology 2022Quote: ... 3.7 g/l sodium bicarbonate and sodium pyruvate) (SH30243.01, Hyclone GE) supplemented with 10% FBS (10270-106, Gibco-Thermo Fisher Scientific) and 1% penicillin-streptomycin (A001A ...
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bioRxiv - Plant Biology 2021Quote: ... The flow-through was transferred to two 1.5-ml tubes (each containing 250 μl flow-through) and 625 µl ethanol, 25 µl NaAc (3 M, pH 5.0) and 1 µl glycogen (Invitrogen, Cat. 10814010) was added ...
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bioRxiv - Developmental Biology 2021Quote: 6 dpf larvae produced from incrossing heterozygous parents were euthanised with an overdose of MS-222 (300mg/L) and the tails were removed for genotyping while the head and remaining body were stored in RNALater™ (Invitrogen). Total RNA was extracted with Trizol reagent (Fisher ...
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bioRxiv - Microbiology 2020Quote: ... medium was changed to F12 medium supplemented with 1% L-glutamine and 10% Fetalclone III serum (FCl-III) (Thermo Fisher Scientific). A total of 1.6 μg of Cas9 Nuclease Expression Plasmid (Dharmacon ...
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bioRxiv - Neuroscience 2020Quote: HEK293T cells were seeded into 12-well culture plates containing coverslips treated with poly-L-lysine and transfected the next day with 1g of each construct using Lipofectamine 2000 (ThermoFisher Scientific) for 18 hours ...
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bioRxiv - Neuroscience 2021Quote: ... using 100 μg custom monoclonal anti-GFP antibodies (50 μg of clone 19C8 with 50 μg of clone 19F7) bound to biotinylated Protein L (Pierce, Thermo Fisher) coated streptavidin-coated magnetic beads (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 cells (ATCC®, C1008) were grown in MEM/EBSS with L-Glutamine (Hyclone) supplemented with 1X non-essential amino acids (Gibco), 1 mM sodium pyruvate (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... they were collected and re-plated on adherent dishes treated with 10 µg/mL Poly-L-ornithine and 2.5 µg/mL laminin (Thermo Fisher Scientific). After passages using Accutase ...
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bioRxiv - Microbiology 2020Quote: ... The purified amplicons were sequenced in both forward and reverse directions using Big Dye Terminator v3.1 Cycle Sequencing kit on Applied Biosystems 3730 x l Genetic Analyzer (Applied Biosystems, USA). The ymdB gene sequences were compared with existing sequences from the NCBI GenBank database using the BLAST algorithm (Altschul S.F et al. ...
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bioRxiv - Cell Biology 2021Quote: ... then incubated with 25 µL of the Click-iT EdU detection mix (prepared as per manufacturer’s instructions using Alex Fluor 455®; Life Technologies, ThermoFisher) for 1 hr in the dark ...
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bioRxiv - Bioengineering 2021Quote: ... Ab constructs or anti-RSV IgG1 control (4 μL) was added to diluted and pH-adjusted CVM (60 μL) and mixed well in a CultureWell™ chamber slide (Invitrogen) followed by the addition of 1 x 106 PM sperm mL-1 of fluorescently labeled sperm (4 μL) ...
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bioRxiv - Immunology 2020Quote: ... 1.5 g/L sodium bicarbonate and 20 lg/mL gentamycin) and fetal bovine serum (FBS) were purchased from Gibco (Carlsbad, CA). Purified anti-mouse CD16/32 ...
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bioRxiv - Bioengineering 2021Quote: Ovaries from 6 to 8 day-old CBA × C57BL/6 (F1) mice were collected and transferred to Leibovitz L-15 media (P/N 11415, Gibco, USA). The ovaries were dissected into 2–4 pieces and transferred into maintenance media (α-MEM ...
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bioRxiv - Neuroscience 2020Quote: ... highly cross-adsorbed AlexaFluor A594 for CD31 and donkey anti-rabbit IgG (H+L) highly cross-adsorbed AlexaFluor A647 for NTSR2 (Life Technologies), diluted in blocking solution at 1:800 ...
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bioRxiv - Neuroscience 2020Quote: ... coverslips were washed three times in PB 0,12 M under agitation at RT and incubated with goat anti-rabbit IgG (H+L) highly cross-adsorbed AlexaFluor A488 (1:800, Life Technologies) diluted in the blocking solution for 2H ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5 µl of 20× primer/probe suspension and 4.5 µl of cDNA diluted in DNase/RNase-free water (10977015, Thermo Fisher Scientific). The thermocycler program consisted of an initial UDG incubation at 50°C for 2 min ...
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with 1 μl of the 1:105 diluted ERCC spike-in mix and 1 μl (50 μM) of random hexamer primer (ThermoFisher 3005) for first strand cDNA synthesis ...