Labshake search
Citations for Thermo Fisher :
8251 - 8300 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2019Quote: ... Thirty micrograms denatured protein of each sample was mixed with SDS buffer and loaded onto a pre-cast 4-12% NuPAGE Bis-Tris gel (Invitrogen, USA). Later ...
-
bioRxiv - Microbiology 2019Quote: ... Reaction products were separated by agarose gel electrophoresis and the amplicon derived from the extended array was purified from the gel using a GeneJET Extraction Kit (ThermoFisher Scientifc) and sequenced on a NextSeq 500 system.
-
bioRxiv - Microbiology 2019Quote: ... Final cDNA libraries with ligated adaptors were size-selected to fall between 250 and 500 bp using the E-Gel® electrophoresis system (Invitrogen). cDNA quantity was measured using the Quant-iT™ PicoGreen® dsDNA Assay Kit (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein-RNA complexes were denatured at 75 °C for 15 mins and run on 4%–12% NuPAGE Bis-Tris gels in NuPAGE MOPS running buffer (all Thermo Fisher) at 150V for 1.5 h ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... PCR products were visualized by agarose gel electrophoresis and nucleic acid staining with SYBR safe DNA stain (S33102, Thermo Fisher Scientific), and imaged with an EZ Gel Documentation System (Bio-Rad ...
-
bioRxiv - Neuroscience 2019Quote: ... A total of 30 µg of protein per sample was separated on 4-12% Bis-Tris gradient gels in MOPS SDS running buffer (Thermo Fisher) at 100 V for 3 h followed by transfer to nitrocellulose membrane at 120 V for 1.5 h at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... Total isolated DNA was visualized on a 1% agarose gel and quantified using the NanoDrop 2000 (Thermo Scientific, Waltham, MA, USA) and Qubit dsDNA HS Assay kit (Thermo Scientific).
-
bioRxiv - Microbiology 2019Quote: ... All samples were checked for quality and quantity with gel electrophoresis and fluorescence-based analysis (Qubit 2.0, Thermo Fisher Scientific, USA) before being used in a second PCR step with barcoded B341 forward primer and reverse B785 primer containing the P1 adapter ...
-
bioRxiv - Cell Biology 2019Quote: ... 50 μg of enriched mitochondria were resolved approximately 6 cm into a pre-cast 4–12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific). The lane was excised and cut in 8 approximately equal chunks and the proteins reduced ...
-
bioRxiv - Developmental Biology 2019Quote: ... we eluted proteins and analyzed samples by electrophoresis on 4%–12% NuPAGE SDS-PAGE gels with MOPS SDS Running buffer (Life Technologies) and corresponding LDS buffer NuPage (Invitrogen ...
-
bioRxiv - Neuroscience 2019Quote: Brain and cell culture lysates containing 50 μg of protein were loaded into 10% SDS/PAGE gels and transferred onto methanol-activated PVDF membranes (Thermo Scientific). Membranes with transferred proteins were blocked/permeabilized for 1 hour in 5% BSA in TBS and incubated with different antibodies (mouse anti-pMLKL ...
-
bioRxiv - Biochemistry 2019Quote: ... Samples were further diluted with SDS-loading buffer and SDS-PAGE was performed using NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen). Proteins were tranferred to nitrocellulose membrane with iBlot 2 Dry Blotting System (Thermos Fisher ...
-
bioRxiv - Bioengineering 2019Quote: ... final concentrations) were loaded per sample in each well of a NuPAGE 10 % Bis-Tris protein gel (ThermoFisher #NP0301BOX, Reinach, Switzerland). Gel electrophoresis was done for 1.5 h at 130 V using a Mini Gel Tank filled with MOPS 1× buffer ...
-
bioRxiv - Plant Biology 2019Quote: ... Digested peptides were extracted from the gel by peptide extraction buffer (50% acetonitrile, 0.1% formic acid) and dried in a vacuum concentrator (Thermo Fisher Scientific). Peptides were resuspended in 0.1% formic acid and desalted using C18 ZipTips (Millipore ...
-
bioRxiv - Biochemistry 2020Quote: ... Samples were analyzed by 20 % denaturing polyacrylamide gel electrophoresis as above and stained with SYBR Gold (Thermo Fisher Scientific, Waltham, MA).
-
bioRxiv - Genetics 2020Quote: ... Samples were heated at 70°C for 10 minutes for denaturation and loaded onto a precast 4-12% mini bis-tris gel (Thermo Fisher) using the XCell SureLock Mini-Cell system (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2020Quote: SDS-PAGE was performed using NuPAGE Novex 4-12% Bis-Tris gels according to the instructions of the manufacturer (Life Technologies). Gels were stained with Coomassie Blue (Life Technologies) ...
-
bioRxiv - Microbiology 2019Quote: ... The proteins were mixed with sample buffer and G250 loading dye and added to a 4-12% Bis-Tris NativePAGETM gel (Life Technologies). BN-PAGE gels were run for 2.5 hours at 150 V using the NativePAGETM running buffer (Life Technologies ...
-
bioRxiv - Molecular Biology 2019Quote: ... to 100 mM final concentration and heated for 10 min at 70°C before being loaded on SDS-PAGE gels (Life Technologies).
-
bioRxiv - Synthetic Biology 2020Quote: The size of the PCR products was verified on an TAE agarose gel (1% w/v) using SybrSafe staining (Thermo Fisher). The BenchTop 1-kb DNA Ladder from Promega was used ...
-
bioRxiv - Pathology 2020Quote: ... and resolved on 4-12% Bis-Tris Plus Bolt™ gels at 4-5 ug of protein per lane in MES SDS Running Buffer (Invitrogen). Target-protein signal was detected with Horseradish Peroxidase (HRP ...
-
bioRxiv - Biochemistry 2020Quote: The gel was run for 2-3 cm and stained with colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific) after which each lane was cut out ...
-
bioRxiv - Cancer Biology 2020Quote: ... Samples were heated to 95 °C for 5 min and cooled on ice prior to loading on NuPAGE 4-12% Bis-Tris gels (Thermo Fisher). SDS-PAGE was performed of all compared samples in parallel according to the manufacturer’s specification ...
-
bioRxiv - Bioengineering 2020Quote: ... The products were gel purified and quantified using the Qubit 3.0 fluorometer with the dsDNA HS assay kit (Thermo Fisher Scientific). Samples were sequenced on an Illumina MiSeq.
-
bioRxiv - Developmental Biology 2020Quote: ... The gel was fixed with 40% methanol/10% acetic acid and then stained for 1 h using colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). After in-gel digestion (Noordstra et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein lysates were separated on tris-glycine or tris-acetate SDS-PAGE gels and transferred to a nitrocellulose membrane using the iBlot 2 dry blotting system (Invitrogen IB21001). The following primary antibodies where used for western-blotting at 1/1000 dilution ...
-
bioRxiv - Systems Biology 2021Quote: ... Aliquots of 15 μg of total protein extract from each sample (3 strains x 4 conditions x 3 replicates) and the three bulks were separated on onedimensional SDS-PAGE short-migration gels (11×1 cm lanes, Invitrogen, NP321BOX). Yeast proteins digestion was performed on excised bands from gel gradient and digested peptides of UPS2 (Sigma ...
-
bioRxiv - Plant Biology 2021Quote: ... Proteins were detected with a Gel Doc XR+ Gel Documentation System by using ECL SuperSignal West Femto Maximum Sensitivity Substrate and ECL Western Blotting substrate (Thermo Scientific) in a ratio of 2:1.
-
bioRxiv - Molecular Biology 2020Quote: ... In-gel digestion was performed using trypsin and resulting peptides were extracted from gel pieces using acetonitrile and trifluoroacetic acid and finally purified on PepClean C-18 Spin columns (Thermo Scientific). Liquid chromatography tandem mass spectrometry (LC-MS/MS ...
-
bioRxiv - Plant Biology 2021Quote: ... and the quality and quantity of the sample was assessed using agarose gel electrophoresis and a fluorometric procedure (Qubit Fluorometer, Invitrogen, USA) by the Beijing Genomics Institute (BGI ...
-
bioRxiv - Microbiology 2021Quote: ... separated through 10-12.5% separating polyacrylamide mini-gels and then visualized by GelCode Blue Stain Reagent (ThermoFisher Scientific, Grand Island, NY). Recombinant proteins expressed in E ...
-
bioRxiv - Biochemistry 2020Quote: ... and lysates containing 30 µg of protein were electrophoresed on a NuPageTM 4–12% Bis-Tris Protein gel (Thermo Fisher Scientific). Proteins were transferred to a PVDF membrane (Amersham) ...
-
bioRxiv - Pathology 2020Quote: ... Whole lanes containing TbGPI3 and wild type cell lines samples were cut identically into 3 slices and the gel pieces were dried in Speed-vac (Thermo Scientific) for in-gel reduction with 0.01 M dithiothreitol and alkylation with 0.05 M iodoacetamide (Sigma ...
-
bioRxiv - Synthetic Biology 2021Quote: ... PCR amplicons were size-verified by electrophoresis on a 0.8% TAE agarose gel stained with SYBR Safe (Thermo Scientific, Waltham, MA) and then excised and purified using the Wizard® SV Gel and PCR Clean-Up System kit (Promega ...
-
bioRxiv - Immunology 2020Quote: ... 75 μg of total proteins were separated by 15 % SDS–polyacrylamide gel electrophoresis and afterwards transferred onto polyvinylidene difluoride membranes using a transblot transfer chamber (X-Cell SureLock™, Invitrogen Novex Mini-cell ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were heated in LDS (lithium dodecyl sulfate) loading buffer then loaded into 4-12% bis-tris gels (Thermo Scientific #NW04122BOX). When blotting for DNA-PKcs ...
-
bioRxiv - Molecular Biology 2021Quote: ... Input controls and 80% of the IPed protein-RNA complexes were run on a NuPAGE 4-12% Bis-Tris Plus Gels (Invitrogen, NP0321BOX), transferred to nitrocellulose membrane (Invitrogen ...
-
bioRxiv - Microbiology 2020Quote: ... The quality and quantity of the DNA extraction were evaluated by 1% agarose gel electrophoresis and Qubit 3.10 Fluorometer (Invitrogen, Life, USA). The good-quality DNA was first sheared randomly to fragments of around 500bp by Focused-ultrasonicattor (Covaris M220 ...
-
bioRxiv - Microbiology 2021Quote: Treponemes harvested at passage #15 (Week 20 post-transformation) were resuspended in SDS-PAGE sample buffer and proteins were separated on a 12% pre-made acrylamide gel (Thermo Fisher) to evaluate the expression of the KanR protein using mass spectrometry after gel band excision and digestion (see protocol below).
-
bioRxiv - Immunology 2020Quote: Proteins were separated by SDS-PAGE (Bis-Tris NuPAGE 12% gel (CALR immunoblot, 10% Bis-Tris NuPAGE gel), ThermoFisher) and transferred to a PVDF membrane (Amersham Hybond-P, GE Healthcare) (CALR, nitrocellulose, Invitrogen) at 25 V for 12 min by using the semidry Trans-Blot Turbo (BioRad ...
-
bioRxiv - Genomics 2019Quote: ... Completed reactions were run on 5% or 4-20% native acrylamide gel and transferred blots were developed using LightShift Chemiluminescent EMSA kit (Thermo Scientific) and imaged on Chemidoc Touch (Bio-Rad) ...
-
All-trans retinoic acid (ATRA) reduces proliferative capacity and Brachyury levels in chordoma cellsbioRxiv - Cancer Biology 2020Quote: ... Samples were denatured in Bolt LDS Sample buffer and Reducing Agent and run on an SDS-polyacrylamide pre cast gel (Bolt 4-12% Bis-Tris Plus, Life Technologies). Samples were transferred onto PVDF membrane in Towbin buffer (10% methanol ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Cells were lysed in Laemmli buffer and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to a polyvinylidene fluoride membrane (ThermoFisher, 88518). Membranes were blocked with 5% milk in TBST for 1h ...
-
bioRxiv - Cancer Biology 2020Quote: ... 12 µg of total protein was loaded per well and resolved in NuPAGE Novex 4-12% Bis-Tris Gels (ThermoFisher Scientific). Proteins were transferred to nitrocellulose membrane ...
-
bioRxiv - Developmental Biology 2019Quote: Conventional interactome analysis of the IP samples was performed as described before [57] with the following changes: The enriched proteins were separated by SDS-PAGE with a 4-12% Bis-Tris protein gel (NuPAGE, Thermo Scientific) and stained with Colloidal Blue Staining Kit (Life Technologies) ...
-
bioRxiv - Genetics 2020Quote: ... Cells were heated for 10 min at 95 °C and separated on a 4-12 % NuPAGE Bis –Tris precasted PAGE gel (Thermo Scientific). Samples were run at 180 V for 10 min and processed by in-gel digestion (78) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein concentration was measured with the BCA Protein Assay Kit (Pierce Biotechnology) and equal amounts of protein were separated on 4–12% Tris-Glycine Gels (Thermo Scientific) and transferred onto Immobilon-P PVDF membranes (Merck Millipore) ...
-
bioRxiv - Immunology 2020Quote: ... IL-33 was also measured in CMT-64 supernatants by western blot – supernatants were ran on 4-12% NuPAGE gels (ThermoFisher Scientific) under reducing conditions ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Gels were run at 260 V (10 V/cm) for 10-20 min and visualised by SYBR Safe staining (Thermo Fisher).
-
bioRxiv - Molecular Biology 2019Quote: ... the samples were boiled at 95°C for 5 minutes before loading 1 % on a 10 % Tris-Glycine SDS-PAGE gel with 10 µL PageRuler Plus Prestained Protein Ladder (Thermo Scientific). After approximately 1 ½ hours electrophoresis proteins were immobilized on an Immobilon-P Transfer Membrane (EMD Milipore ...