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Citations for Thermo Fisher :
8251 - 8300 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Inc and utilized for transfection of HNSCC cell lines with a final concentration of 30 nmol/L siRNA with Lipofectamine RNAiMAX (Life Technologies). ROBO1 or EPHA2 expression vectors were constructed by fusing PCR fragments containing full length ROBO1 or EPHA2 upstream to eGFP ...
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bioRxiv - Neuroscience 2019Quote: ... containing either 350 μL dH2O added to a piece of Kim wipe (starved condition) or 350 μL of 0.2 M sucrose (Acros Organics #177140050) added to a piece of Kim wipe (non-starved condition).
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bioRxiv - Cancer Biology 2019Quote: ... the cells were washed with PBS and incubated with CM-H2DCFDA (1 μmol/L in PBS; Molecular Probes; Eugene, OR, USA) for 10 minutes ...
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bioRxiv - Bioengineering 2019Quote: ... Pups were extracted from the ovaries and immersed in a 10 cm petri dish containing cold L-15 media (Life Technologies). The pups were decapitated ...
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bioRxiv - Pathology 2020Quote: HCAEC cultures were transfected with siRNA sequences that are known to silence NOTCH4 (L-011883-00, Dharmacon) using the Lipofectamine® RNAiMAX transfection system (13778-150, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 1.7×107 cells were seeded into 175 cm² cell culture flasks in RPMI 1640 media with L-glutamine (Thermo Fisher Scientific) containing 10 % heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2019Quote: Protein concentration in g ⋅ L−1 of human serum was calculated using PierceTM Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were incubated for 1 hour at room temperature in appropriate secondary antibodies: Goat anti-mouse IgG (H+L) HRP (ThermoFisher Scientific) or Goat anti-rabbit IgG (H+L ...
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bioRxiv - Genomics 2019Quote: ... 5 μl of RNase A/T1 Mix (RNase A at 500 U/mL and RNase T1 at 20000 U/mL, Ambion AM2286) diluted 100 times were added to each tube ...
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bioRxiv - Bioengineering 2019Quote: ... were plated at low density on poly-L-lysine coated coverslips (18 mm) and transfected the following day using either Lipofectamine 2000 or 3000 (Thermo Fisher). 24-48 hrs after transfection ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... cells were purchased from ATCC (ATCC: CRL-2128) and grown in DMEM/Ham’s F-12 medium containing L-glutamine and 15 mM HEPES (GIBCO, Paisley, UK) supplemented with 5% NU-I serum (Becton Dickinson ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder, Applied Biosystems, in 10 μL of Hi-Di formamide, Applied Biosystems) and incubated at room temperature for 20 minutes ...
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bioRxiv - Genetics 2021Quote: DNA and total RNA was extracted from wild-type (Nicotiana tobacum L. cv. xanthi) and tobrhGAA seeds by kits (Thermo Scientific GeneJET Plant RNA purification kit-K0801 ...
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bioRxiv - Genomics 2021Quote: ... 2 wells of a 24 well plate were prepared and contained 2 mL RPMI-1640 media with 25 mM HEPES and L-glutamine (ThermoFisher Scientific) + 10% heat inactivated FBS (ThermoFisher Scientific).
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bioRxiv - Immunology 2021Quote: ... Triton X-100 with 5 % normal-goat-serum and unconjugated F(ab′)2 goat anti-mouse IgG (H+L) antibody (1:500, Thermo Scientific), at 4 °C for 2 h ...
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bioRxiv - Immunology 2021Quote: ... with unconjugated rabbit anti-human phosphorylated NF-κB p65 (Clone: Ser536 93H1) followed by goat anti-rabbit IgG (H+L) Alexa Fluor 488 (Thermo Fisher). For p38 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice in PBS then suspended in goat anti-mouse IgG (H&L) cross-adsorbed secondary antibody Alexa Fluor 488 (2 mg mL−1; Thermofisher Scientific) in PBS 0.1 % BSA for 45 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... the CST staining protocol was followed with mouse anti-human phosphorylated p38 MAPK (Clone: Thr180/Thr182) used with goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 (Thermo Fisher) as a secondary ...
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bioRxiv - Microbiology 2021Quote: ... 400 µL clear aqueous phase was aspirated from the top layer of the plate and transferred to a deep well plate containing 200 µL 100% isopropanol and 10 µL RNA binding beads from the MagMAX-96 for Microarrays Total RNA Isolation Kit (ThermoFisher, #AM1839). The Nanocell system then transferred this plate to a connected KingFisher Flex ...
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bioRxiv - Neuroscience 2020Quote: ... and donkey anti-mouse IgG (H+L, Highly Cross-Adsorbed, Alexa Fluor 488; 1:500, catalog: A-21202, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... biotinylated anti-mouse IgG (H+L) made in horse and biotinylated anti-mouse IgG2a made in goat (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... biotinylated anti-mouse IgG (H+L) made in horse and biotinylated anti-mouse IgG2a made in goat (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Biophysics 2021Quote: ... Cover glasses were mounted on the imaging chamber and DMEM medium was replaced by pre-warmed L-15 imaging medium (cat. no. 11415049, Gibco, Fisher scientific). Imaging was done at 37°C on a 3i second generation lattice-light-sheet microscope with a 0.71 NA LWD WI objective for excitation and a 1.1 NA WI objective for imaging and equipped with 2 Hamamatsu ORCA-Flash 4.0v3 sCMOS cameras for simultaneous dual color imaging providing a 62.5x magnification with 230×230×370 nm (x-y-z ...
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bioRxiv - Developmental Biology 2021Quote: ... Slides were washed 3 times in 1× PBS at RT and incubated in the combination with secondary antibodies Alexa 488-conjugated goat anti-rabbit IgG (H+L) (Molecular Probes) and Alexa-594-conjugated goat anti-chicken IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... Ribosomes were immunoprecipitated using anti-EGFP antibodies (an equal mixture of clones 19C8 and 19F7 from Monoclonal Antibody Core Facility at Memorial Sloan-Kettering cancer center) that were previously conjugated to Protein L coated magnetic beads (ThermoFisher Scientific) for 16-h in 4°C with rotating ...
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bioRxiv - Biochemistry 2021Quote: ... GP-1 was detected by incubating the slide with 2 μg/mL of Alexa Fluor 633 goat-anti-mouse (H+L) antibody (Invitrogen A21050) for 3 h ...
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bioRxiv - Bioengineering 2021Quote: ... Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol red-free DMEM + 10% FBS + 2 mM L-glutamine (Thermo Fisher). Cells were incubated with 100 nM TO-1 in HBSS starting 30 minutes prior to imaging and for the duration of the imaging period ...
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bioRxiv - Bioengineering 2021Quote: CHO cells and CHO EVs for SEM were fixed in 4% EM grade glutaraldehyde overnight on poly-L-lysine coated glass coverslips (Fisher Scientific). Samples were processed for SEM imaging by treating the samples to 1% osmium tetroxide for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... followed by washes and detection with Alexa488-conjugated goat anti-mouse IgG(H+L) Cross-Adsorbed Secondary Antibody (Invitrogen A-11001), essentially as described previously (Siggers et al ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed in TBST and the bound antibody was detected after 1 h incubation at RT with HRP-Goat Anti-Rabbit IgG (H-L) (ThermoFisher, G21234) diluted 1:4000 in 2% blocking buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Then 50 μL of stop solution was added and the absorbance was read at 450 nm by using Multiskan Go spectrophotometer (Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: Spinal cord tissue from stage HH 25-26 (Day 5-6) chick embryos (Venkateshwara hatcheries Ltd.) were dissected out in L-15 medium (Gibco, HiMedia) with added 1x penstrep (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... L = 20 mm) and separated on a C18-EasySpray analytical column (particle size = 2 μm, ID = 75 μm, L = 50 cm, ThermoFisher Scientific) using a nano-HPLC (Dionex U3000 RSLCnano) ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μl of sample was loaded at a rate of 0.3 μl/min in 0.1% FA onto a trap column (C18, Acclaim PepMap 100 75 um x 2 cm; Thermo Fisher Scientific) and eluted across a fritless analytical resolving column (C18 ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems]) and incubated at room temperature for 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... 338-AC, 100 ng/mL) in DE medium (RPMI 1640, 1% Penicillin/Streptomycin, 1% L-Glutamine (Gibco, ref. 25-030-081). DE differentiation was induced by threating MES differentiated cultures for 2 days with ACTIVIN A (R&D ...
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bioRxiv - Cell Biology 2021Quote: ... buffer was aspirated from the bottom compartment and replaced with warm explant medium (15% fetal bovine serum and 0.02% L-ascorbic acid in Waymouth’s media (Thermo Fisher Scientific #11220035)) (based on Farbman ...
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bioRxiv - Cell Biology 2021Quote: ... and then replaced with imaging media consisting of phenol red-free DMEM with L-glutamine and HEPES (Thermo Fisher Scientific 21063029) supplemented with 10% heat-inactivated fetal bovine serum (Sigma F4135 ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with an Alexa 594 Goat Anti-Rabbit IgG (H+L) (1:500) (Thermo Fisher Scientific, Waltham, MA, USA) and Alexa 488 Phalloidin (1:50 ...
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bioRxiv - Microbiology 2020Quote: ... One microliter of each sample was mixed with 15.4 μl of highly deionized formamide and 0.1 μl of HD400 ROX-labeled DNA size marker (both reagents from Thermo Fisher Scientific).
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bioRxiv - Genetics 2020Quote: ... then incubated with DAPI (1:5000) and Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibodies (1:200; Thermo Fisher). After incubation ...
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bioRxiv - Genomics 2021Quote: ... as well as nasal and oral swab/scrapings were placed in 25 mL of RPMI-1640 media with 25 mM HEPES and L-glutamine (ThermoFisher Scientific) + 10% heat inactivated FBS (ThermoFisher Scientific ...
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bioRxiv - Genomics 2021Quote: Cryopreserved stocks of the KH004 parent and the Mal31×KH004 uncloned bulk recombinant progeny pool and were thawed and grown in complete media (CM) [RPMI 1640 with L-glutamine (Gibco, Life Technologies), 50 mg/L hypoxanthine (Calbiochem ...
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bioRxiv - Bioengineering 2021Quote: MDCK.2 wild type canine kidney epithelial cells (ATCC) were cultured in customized media consisting of low-glucose (1 g/L) DMEM with phenol red (Gibco, USA), 1 g/L sodium bicarbonate ...
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bioRxiv - Microbiology 2021Quote: ... 10µl of RV- C15 stock or 100µL of experimental sample was first treated with 1u RNase A (#EN053; Thermo) and 1u DNase I (#18047019; Invitrogen – Thermo Scientific) for 1 hr at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were stained with DAPI (1 mg/l) for 10 min and the slides washed five times with PBS before mounting with ProLong™ Gold Antifade Mountant (ThermoFisher). Sections were imaged on a Nikon 90i fluorescence microscope with NIS-Elements software ...
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bioRxiv - Microbiology 2021Quote: ... Cells were further permeabilized with 0.2% Triton X-100 and incubated with cross-reactive rabbit sera anti-SARS-CoV-2-N for 1 hour at RT before adding Alexa Fluor 488 goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (Life Technologies). The fluorescence density of SARS-CoV-2 infected cells was acquired using a Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Cells were further permeabilized with 0.2% Triton X-100 and incubated with cross-reactive rabbit sera anti-SARS-CoV-2-N for 1 hour at RT before adding an Alexa Fluor 488 goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (Life Technologies). The fluorescence density of SARS-CoV-2 infected cells were scanned using a Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed 3 x 5 min with 500 mml per well of TBS and incubated at 37°C for 1h with 250ul of a mixture containing goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody conjugated with Alexa Fluor plus 488 (Invitrogen, USA) and goat anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2021Quote: ... 1.5 µl of 3 ng µl-1 cDNA was used as template in the QuantStudio 5 Real-Time PCR system (Applied Biosystems). Amplifications were performed with 5 μl of SYBR® green JumpStart Taq ReadyMix (Sigma-Aldrich ...