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Citations for Thermo Fisher :
8201 - 8250 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 10 mM Bis-Tris pH 7.0) and resolved on a Novex NativePage 4–16% Bis-Tris Protein Gel (Invitrogen CAT# BN1004BOX) run at 150 V for 3 hours ...
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bioRxiv - Cell Biology 2022Quote: Proteins were separated by SDS-PAGE using Bis-Tris 4–12% gradient gels in MES or MOPS buffers (Lifetech) in a Mini Blot Tank (Thermo Fisher). Western blot analyses were performed using a wet transfer Criterion Blotter (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... 10 mg of protein from the heart or liver or 20 mg of protein from the skin was resolved on a 4 %-12 % gradient bis-tris gel (NP0322, Thermo Fisher). The proteins were transferred to a PVDF membrane (1704156 ...
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bioRxiv - Cell Biology 2022Quote: ... The bottom of the PAGE gel on which the chromatin samples were resolved was cut off and stained with Colloidal Coomassie (SimplyBlue, Life Technologies) to stain histones which provide loading controls and indications of sample contamination with egg extract (cytoplasm).
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bioRxiv - Cell Biology 2022Quote: ... The quality and quantity of DNA isolation across the 96 well plate were measured using agarose gel electrophoresis and Nanodrop (ThermoFisher Scientific), respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were mixed with 6X sample buffer containing β-ME and were separated by SDS-PAGE electrophoresis using NuPAGE 4-12% Bis-Tris gels (Invitrogen), and transferred onto the 0.45 μm nitrocellulose membranes ...
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bioRxiv - Neuroscience 2021Quote: Western blotting was performed (Uchida et al., 2017) using equal amounts of protein separated on 12% Bis-Tris gels (Life Technologies) and transblotted onto polyvinylidene difluoride membranes (GE Healthcare Bio-Sciences) ...
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bioRxiv - Microbiology 2020Quote: ... and samples were heated to 95°C for 5 minutes and subjected to electrophoresis in 12% NuPAGE Bis-Tris gels (Life Technologies). Gels were transferred to nitrocellulose membranes (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... was mixed with 20 μl of each sample (60 to 80 μg of protein) and loaded onto a BN-PAGE gradient gel (4 to 16%) (Invitrogen, Inc.). One ml of 2X BN sample buffer consisted of 1.5 M 6-aminohexanoic acid ...
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bioRxiv - Developmental Biology 2020Quote: ... The gel was fixed with 40% Methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain, Thermo Scientific). Each lane from the gel was cut and placed in 1.5 ml tubes ...
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bioRxiv - Neuroscience 2020Quote: ... sample buffer containing 40 mM DTT and denatured at 70°C for 10 min prior to loading on 10% NuPAGE Novex midi gels (Thermo Fisher). Gels were run using MES [2-(N-morpholino)ethane sulfonic acid] running buffer at 150-160 V (110 V for the first 15 min ...
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bioRxiv - Microbiology 2020Quote: ... the peptides from each gel slice in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Microbiology 2019Quote: Viral and cell lysates were resuspended in sodium dodecyl sulfate (SDS) sample buffer and separated by electrophoresis on Bolt 4-12% Bis-Tris Plus gels (Life Technologies), blotted onto nitrocellulose membranes and probed overnight at 4°C with the following antibodies in Odyssey Blocking Buffer (LI-COR) ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride membrane [36] and detected with the anti-His (C-terminal)-HRP antibody (Thermo Fisher/Invitrogen) according to the manufacturer's instructions ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: 10 μg of total RNA isolated from the combined fractions was resolved by 15% TBE-Urea gel (Thermo Fisher Scientific, EC6885BOX) and visualized with SYBR™ Gold Nucleic Acid Gel Stain(Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... PDH+DMTMM cross-linking was performed for 45 min at 37 °C followed by removal of the reagents by gel filtration (Zeba spin desalting columns, ThermoFisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... RAPD amplicons were visualized on a 1% agarose gel that included a GeneRuler 1kb plus DNA ladder (Thermo Scientific, Waltham, MA) and a RAPD performed with PAO1 genomic DNA to aid in comparison of clinical isolates’ patterns ...
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bioRxiv - Cell Biology 2021Quote: ... the 20μL samples were diluted in 40 μL of water and run on a 0.8% agarose gel for 1 hour at 70 Volts in TAE buffer and post-stained with SYBR Gold (Invitrogen, cat# S11494) for 30 minutes in TAE buffer ...
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bioRxiv - Microbiology 2021Quote: ... and was then analyzed by electrophoresis on a 1% agarose gel as well as a Qubit 2.0 (Thermo Scientific, Waltham, USA). DNA libraries were generated using a NEBNext UltraTM II DNA Library Prep Kit (NEB ...
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bioRxiv - Molecular Biology 2020Quote: Cell lysates with normalized total protein concentrations were mixed with SDS sample buffer and boiled, separated by SDS-PAGE (Novex 4-12% Tris-Glycine Mini Protein Gels, 1.0mm, 15 well (Life Technologies, EC6025)) ...
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bioRxiv - Microbiology 2021Quote: ... 10ul of lysate or purified supernatant samples were run on a 4-12% Bis-Tris SDS-PAGE Gel (Invitrogen, Cat #NW04120BOX) with subsequent transfer to 0.45 μm PVDF membrane ...
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bioRxiv - Systems Biology 2020Quote: ... The integrity of the extracted genomic DNA was analyzed by running it on an agarose gel and the quality was assessed and quantified using Multiskan GO (Thermo Scientific). The genomic DNA library was prepared using Illumina TruSeq DNA Nano Kit ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of protein (20-50 μg) were loaded and were separated by electrophoresis in 4 to 12% Bis-Tris Gel (ThermoFisher Scientific), transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were then washed twice (30 min each) in transfer buffer containing 1 mM EDTA (1×NuPAGE Transfer Buffer, Invitrogen, NP00061). Western blot transfer on PVDF membranes was performed overnight at 4°C in 1×NuPAGE Transfer Buffer containing 10% methanol and 5 mM sodium bisulfite ...
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bioRxiv - Neuroscience 2021Quote: ... 10-30ug of protein was incubated with 1x reducing agent and 1x LDS sample buffer at 70°C for 10 minutes before immediately being loaded onto a BOLT 4-16% Bis-Tris gel (ThermoFisher Scientific) in 1x MES buffer ...
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bioRxiv - Neuroscience 2021Quote: Proteins from tissue preparations were solubilized in Laemmli buffer before being stacked in the top of a 4–12% NuPAGE gel (Life Technologies), stained with R-250 Coomassie blue (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The resulting amplification products were verified by gel electrophoresis and cleaning and normalization of individual PCR products was performed with SequalPrep™ Normalization Plate (96) Kit (Invitrogen). The normalized PCR amplicons were mixed ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were separated on 4-12% SDS-PAGE gel (Life Technology) and silver stained using Pierce Silver stain kit (Thermo Fisher).
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bioRxiv - Biochemistry 2020Quote: ... The samples were separated on 4-12% SDS-PAGE gel (Life Technology) and silver stained using Pierce Silver stain kit (Thermo Fisher).
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bioRxiv - Biochemistry 2020Quote: ... The FSE 1D chemical mapping construct′s assembled dsDNA was 2% agarose gel purified by size using blue-light transillumination using SyBrSafe (Invitrogen™) and purified using a Qiagen MinElute Gel Extraction kit prior to IVT.
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bioRxiv - Microbiology 2021Quote: ... Gels were electrophoresed at 60 °C and 70 V for 16 h and visualized with SYBR Gold staining (Life Technologies, NY). Digital photographs of gel images were analyzed using GelComparII v.5.10 (Applied Maths ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates (10 µg of protein/well) were run on 20-well 4-12% NuPage BisTris midi gels in 1X MES buffer (ThermoFisher Scientific) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2021Quote: ... LC-MS/MS analysis of all gel fractions was performed on an LTQ-Orbitrap™ mass spectrometer (Thermo Fisher, Waltham, MA) coupled with a nanoAcquity™ UPLC™-system (Waters ...
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bioRxiv - Systems Biology 2022Quote: ... 20 μg of protein from each sample were loaded in separate wells in a 4-12% Bis-Tris gel (Invitrogen #WG1403A) and ran at 90V using MOPS SDS running buffer (Invitrogen #NP0001) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products (3µl of the initial reaction mixture) were separated on a 1.2% agarose gel stained with SYBR® Safe (Invitrogen™, France). Gel production and electrophoresis were conducted using TAE buffer (40mM Tris-acetate pH 8.0 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Quality was checked by running individual samples on 2% agarose gel stained with ethidium bromide and also with a UV-Vis Spectrometer (NanoDrop 1000, Thermo Scientific). Quantity was checked with a QuBit fluorometer (dsDNA HS ...
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bioRxiv - Microbiology 2022Quote: ... Equal amount of the products from the three fragments of each sample were purified (PureLink Quick Gel Extraction Kit, ThermoFisher Scientific) and mixed ...
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bioRxiv - Immunology 2022Quote: ... Denatured tri-S protein (50 µg total) was separated by SDS-PAGE with a NuPAGE® 4-12% Bis-Tris Gel (1-well, Invitrogen), electro-transferred onto nitrocellulose membranes ...
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bioRxiv - Immunology 2022Quote: ... The quality/purity of the different purified fractions was evaluated by SDS-PAGE using 3–8% Tris– Acetate gels (Life Technologies) under non-reducing conditions followed by silver staining (Silver Stain kit ...
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bioRxiv - Molecular Biology 2022Quote: ... were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) before transferring onto 0.45 μm nitrocellulose membrane (Thermo Scientific™) with a Hoefer TE77X semi-dry transfer unit at 20V for 1 h ...
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bioRxiv - Biophysics 2022Quote: ... with 10 mM KCl or LiCl with Invitrogen 1.0 mm x 12 well 20% TBE-PAGE gels (EC63152BOX, Thermo Fisher Scientific). Gels were pre-run and run for 1 hour (unless otherwise stated ...
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bioRxiv - Cell Biology 2022Quote: ... was added to the supernatant and 100 μg of protein was loaded onto a 3-12% native gel (NativePAGE™ (Invitrogen)) ...
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bioRxiv - Immunology 2022Quote: ... and 3-10μg of total protein cell lysates was run on 4 to 12% NuPAGE Bis-Tris SDS-PAGE gels (Invitrogen by ThermoFisher Scientific) and transferred to polyvinylidene difluoride membranes (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2019Quote: ... Single strands were separated by electrophoresis in a 1.2% (m/V) agarose gel stained with Sybr™ Gold (Thermo Fisher Scientific). After electrophoresis ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were split into two 30 μg aliquots and separated on a Bolt 4-12% Bis-Tris Plus SDS gel (Cat. No. BG04120BOX, Life Technologies) for 2 h at 80 volts ...
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bioRxiv - Zoology 2019Quote: PCR products were run and purified by agarose gel electrophoresis and cloned into the pJET 1.2 cloning vector (Thermo Fisher Scientific). The inserts were sequenced.
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bioRxiv - Biophysics 2019Quote: ... Both the biotinylated and intracellular fractions were normalized to total protein and loaded on a 4-12% Bis-Tris pre-cast gel (Thermo Fisher Scientific ...
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bioRxiv - Zoology 2020Quote: ... The quality of extracted genomic DNA was assessed using gel electrophoreses and the concentration was measured by Nanodrop (ND-1000; Thermo Scientific). The resultant genomic DNA was stored at −20□°C until further analysis.
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bioRxiv - Molecular Biology 2019Quote: ... incubated at 95°C for 5 min and the supernatant loaded onto a either 3-8% Tris-acetate or 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Microbiology 2019Quote: ... The quality and concentration of the PCR products were determined through analysis on a 1% (w/v) agarose gels stained with 1×SYBR® Safe ethidium bromide (Invitrogen) and using the dsDNA HS Assay kit for Qubit fluorometer (Invitrogen) ...