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Citations for Thermo Fisher :
8201 - 8250 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... isolates were revived from glycerol stocks into 500µL liquid LB-L media in wells of 2.0mL 96-well DeepWell™ blocks (ThermoFisher™ Nunc™). The blocks were incubated with shaking until the cultures reached stationary phase ...
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bioRxiv - Molecular Biology 2020Quote: ... We used 1.6 μg of c-Myc monoclonal antibody and 50 μL PanMouse IgG magnetic beads or 1 µl of anti-Flag antibody and 30 µl Protein G magnetic beads (Thermo Scientific). For Cdc5-TAP ChIP ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies were used: Alexa Fluor 488-conjugated goat anti-chicken IgY (H+L) (Invitrogen, A-11039, 1:1000) and Alexa Fluor 555-conjugated donkey anti-rabbit (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were resuspended in 250 µl of PBS and fixed by the addition of an equal volume of PBS containing 2% methanol free formaldehyde (Thermo Scientific Pierce PN28906 ...
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bioRxiv - Cell Biology 2019Quote: ... Serum was used in a 1:100 dilution and Alexa Fluor 488-goat anti-rabbit IgG (H+L) (Invitrogen; A-11034) 1:5,000 ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibodies (Goat anti-mouse IgG1 Alexa Fluor 488 conjugate; Invitrogen A21121, Goat anti-rabbit H+L Alexa Fluor 555 conjugate; Invitrogen A21428) with Hoechst 33342 (Thermo H3570 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed in PBS and incubated with Texas Red goat anti-mouse IgG or Alexa Fluor 488 goat anti-rabbit IgG (H+L, ThermoFisher Scientific) for 1h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... The GST-fused protein was eluted with 1 ml GST elution buffer and the eluted sample was dialyzed overnight at 4°C in 2 l 1x PBS in a Slide-A-Lyzer Dialysis Cassette G2 10 000 MWCO (Thermo Scientific).
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bioRxiv - Cell Biology 2019Quote: ... Nrf1α−/− cells were transfected with 80 nmol/L of the siNrf2 oligonucleotides in the mixture of Lipofectamine 3000 (Invitrogen, California, USA). Thereafter ...
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bioRxiv - Immunology 2021Quote: ... Embryos were then washed with PBST and blocked with 5% goat serum before addition of the secondary antibody (Goat anti-rabbit IgG H&L DyLight 650, 1:200, 84546 Invitrogen, USA) and incubated overnight at 4°C with gentle rocking ...
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bioRxiv - Genetics 2021Quote: ... The sections were rinsed in PBS/Tween buffer before incubating for 60 min in 1:200 goat anti-rat IgG (H+L) antibody conjugated to horseradish peroxide (HRP) (Invitrogen, ThermoFisher Scientific). The slides were then developed in 3,3’ diaminobenzidine (DAB ...
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bioRxiv - Genetics 2021Quote: ... and 1x Pen Strep Glutamine (100 Units/mL Penicillin, 100μg/mL Streptomycin, and 0.292 mg/mL L-glutamine) (Invitrogen cat# 10378-016) for a final volume of 1 mL ...
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bioRxiv - Neuroscience 2021Quote: ... Primary neurons were plated onto poly-L-lysine-coated coverslips for 6-well plates and cultured in MEM medium (Thermo Fisher) containing 2% B27 and 5% FCS ...
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The key features of SARS-CoV-2 leader and NSP1 required for viral escape of NSP1-mediated repressionbioRxiv - Molecular Biology 2021Quote: ... Peptides were pre-concentrated on a 100 μm x 2 cm C18 trapping column for 5 min using 0.1% TFA with a flow rate of 20 μL/min followed by separation on a 75 μm x 50 cm C18 main column (both Acclaim Pepmap nanoviper, Thermo Scientific) with a 60 min (BioID samples ...
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bioRxiv - Evolutionary Biology 2021Quote: ... ∼5 µl of the bacterial colony was transferred into 500 µl and the OD500 was measured on a NanoDrop Spectrometer (Thermo Fisher). Based on the OD reading ...
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bioRxiv - Microbiology 2021Quote: ... was seeded with influent solids harvested from the influent of the RAEBL full-scale WRRF by weekly centrifuging 10 L of a 24-h composite influent sample at 4,000×g using the IEC-Centra-8 centrifuge (Thermo Scientific, USA). The total suspended solids (TSS ...
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bioRxiv - Developmental Biology 2020Quote: ... Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (Abcam, #ab150079, or Thermo Fisher Scientific, #A21247, 1:1000 dilution).
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bioRxiv - Developmental Biology 2021Quote: ... All cell lines were cultured under standard conditions in ES medium (DMEM/Gibco, 15 % FBS/Gibco, 2mM L-glutamine/Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 x 105 – 4 x 105 mESCs were cultured in 60mm Petri dishes in DFNB medium (Neurobasal medium/Gibco, DMEM F12 1:1/Gibco, 1.5 mM L-glutamine/Thermo Fisher Scientific, 1X Penicillin Streptomycin/Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... 30min prior to the measurements the culture medium was exchanged to Leibovitz’s L-15 medium without phenol red (21083027, Thermo Fisher Scientific) and the non-control samples were treated with 5mM sodium arsenite ...
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bioRxiv - Biophysics 2022Quote: ... Directly before the imaging the culture medium was exchanged to Leibovitz’s L-15 Medium without phenol red (21083027, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... from carrageenan injected and non-injected sides were collected separately in dissociation media (L-15 + GlutaMAX growth media supplemented with 24 mM NaHCO3; Life Technologies). Dissected DRG were then incubated in dissociation media containing 1 mg/ml type 1A collagenase (Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... or 13C6 L-Lysine-2HCl (Lys-8) and 13C6 L-Arginine-Hcl (Arg-10) for the metabolic incorporation of ‘light’ and ‘heavy’ stable isotopes respectively (ThermoFisher Scientific). HEK293FT cells were grown in light or heavy SILAC media for at least 10 doublings ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were finally resuspended in adhesion media (MEM, 10% horse serum, 30% glucose, L-glutamine, and penicillin/streptomycin; Life Technologies) and plated in 24- or 6-well plates previously treated with poly-L-lysine 0.1% w/v in water (Brunschwig ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed thrice for 5 min in PBS before incubation with Alexa 594 goat-anti rabbit secondary antibody at 1:1,000 and Alexa Fluor 488 goat anti-human IgG(H+L) at 1:500 (both Invitrogen; in PBS) for 2 h at RT ...
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bioRxiv - Molecular Biology 2021Quote: TeloHAECs were transfected with a pool of Atp6ap2 (Dharmacon, L-013647-01-0005) and non-targeting control-siRNAs (Dharmacon, D-001810-10-05) using Lipofectamine 3000 (ThermoFisher, L3000015) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Sample were loaded via autosampler onto a 20-µL sample loop and injected at 0.3-µL/min onto a 75-µm x 150-mm EASYSpray column (Thermo Fisher Scientific) containing 2-µm C18 beads ...
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bioRxiv - Molecular Biology 2020Quote: ... The secondary antibodies (1:1000) used were: goat anti-mouse IgG(H+L) Alexa Fluor 594 (Thermo Fisher Scientific, A-11005).
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bioRxiv - Molecular Biology 2021Quote: All media contained 2 mM alanyl-L-glutamine dipeptide (GlutaMAX™) and were supplemented with 10 % fetal bovine serum (HyClone, Gibco™ ...
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bioRxiv - Molecular Biology 2021Quote: ... was incubated with 10 µL of mouse polyclonal anti-SmHDAC8 or mouse polyclonal anti-SmRho1 crosslinked to protein-L Agarose beads (Thermo Scientific) overnight at 4°C on a rotator ...
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bioRxiv - Molecular Biology 2021Quote: ... Yeast cells were cultured overnight in 2 mL of YPAD liquid medium (10 g/L Bacto Yeast Extract, #212750, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Dissociated cells were seeded onto coverslips coated with poly-L-lysine and cultured in 500 μL Neurobasal medium containing B27 supplement (Thermo Fisher) and 24 mM KCl at a density of 8-10k cells/well to allow for observation of isolated neurons ...
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bioRxiv - Cell Biology 2022Quote: ... were obtained from the UC Berkeley cell culture facility and were cultured in DMEM (Gibco-Thermo Fisher 1165092 high glucose, +L-glutamine,10% FBS, with the addition of 100 U/ml Pen/Strep-Gibco). Cells were seeded at 5000 cells/ cm2 for passaging ...
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bioRxiv - Immunology 2022Quote: ... 10% foetal calf serum, 10 units/ml penicillin, 10 μg/ml streptomycin and 2 mM L-glutamine, all supplied by Gibco, ThermoFisher UK). All cells were incubated for 72 hours with Myelin Oligodendrocyte Protein (MOG ...
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bioRxiv - Immunology 2022Quote: ... 10% foetal calf serum, 10 units/ml penicillin, 10 μg/ml streptomycin and 2 mM L-glutamine, all supplied by Gibco, ThermoFisher UK). All cells were differentiated for 48 hours in the presence of plate-bound αCD3 (2.5ug/ml ...
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bioRxiv - Immunology 2021Quote: ... FACs sorted tonsil B cell subsets were seeded on to poly-L-lysine coated chamber slides (Nunc Lab-Tek, Thermo Scientific) stimulated ...
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bioRxiv - Immunology 2021Quote: ... FACs sorted tonsil B cell subsets were seeded on to poly-L-lysine coated chamber slides (Nunc Lab-Tek, Thermo Scientific) stimulated ...
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bioRxiv - Cell Biology 2020Quote: ... Differentiation was stimulated by replacing the proliferation medium with differentiation medium (high glucose DMEM with sodium pyruvate, 5% horse serum, 2% L-glutamine, ThermoFisher Scientific). The cells were maintained at 37°C and 5% CO2 throughout differentiation.
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bioRxiv - Systems Biology 2020Quote: ... of the immunodepleted pooled CSF samples were separately dissolved in 100 mM TEAB buffer (625 μL) and labeled with 5 mg of TMT 126 channel reagent (cohort 1 lot# TF266326, cohort 2 lot# SG253447, ThermoFisher Scientific) in anhydrous ACN (256 μL) ...
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bioRxiv - Cell Biology 2019Quote: ... 3 μl of the final reaction was subjected to electrophoresis on a 1.5% agarose gel using 2xRNA loading buffer (Thermo Fisher Scientific). A 1:1 mix of RNA and loading buffer was heated to 65°C for 5 min and then placed on ice to denature any secondary structure of RNA.
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bioRxiv - Cancer Biology 2019Quote: ... BCL2L11 (Bim) siRNA SMARTpool was from Dharmacon (L-004383-00-0005) and BAK siRNA was obtained from Ambion (Life Technologies 4457298).
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bioRxiv - Cell Biology 2019Quote: ... and diluted 1.25 volumes of blood with 1 volume RPMI 1640 medium (HyClone) supplemented with 2mM L-Glutamine (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2019Quote: ... 1-2 µl of sample were analyzed with a hybrid LTQ-Orbitrap Velos or with an Orbitrap Fusion Tribrid Mass Spectrometer (ThermoFisher, USA). For the Velos system ...
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bioRxiv - Cell Biology 2019Quote: ... 100 µL samples were placed in Slide-A-Lyzer mini dialysis cups with a two kDa molecular weight cut-off (ThermoFisher #69553). Samples were dialyzed against a 10 mM Tris pH 7.4 buffer with 150 mM NaCl unless otherwise stated ...
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bioRxiv - Cell Biology 2019Quote: ... genes of interest were cloned into pDONR223 then recombined into destination vectors pHAGE-N-FLAG-HA or pHAGE-N-GFP using L Recombinase (Life Technologies) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The 0.2 mL PCR tubes each containing 50 μL placed into a reaction was allowed to take place in a GeneAmp PCR System 9700 (MTHE 01326 PE Applied Biosystems) thermocycler for 3 hours ...
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bioRxiv - Genetics 2019Quote: ... The samples were washed with PBS containing 0.1% BSA after exposed secondary antibody Alexa Fluor® 488 goat anti-rabbit IgG (H+L, Life Technologies, ref ...
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bioRxiv - Biochemistry 2019Quote: ... T2 cells (174 x CEM.T2) (ATCC® CRL-1992™, distributed by Biological Industries) were cultured in RPMI 1640 medium containing L-glutamine (Gibco, distributed by Rhenium Ltd ...
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bioRxiv - Biochemistry 2019Quote: ... T2 cells (174 x CEM.T2) (ATCC® CRL-1992™) were grown as described above in RPMI 1640 medium containing L-glutamine (Gibco), supplemented with 20% heat inactivated fetal calf serum ...
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bioRxiv - Biochemistry 2019Quote: ... the antibody-coupled beads were washed three times in 100 μl PBS-T before labelling the antibodies with a 150x molar excess of EZ-Link-NHS-PEG4-Biotin (21329, Thermo Scientific) dissolved in DMSO (276855 ...