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Citations for Thermo Fisher :
8151 - 8200 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Purification was confirmed by running the protein on a 4-12% Bis-Tris gel (Novex, Waltham, MA) and performing a Coomassie (PageBlue, Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: Equal amounts of total cell extracts were run under denaturing conditions using NuPAGE Novex 4–12% Bis-Tris gradient gels (Life Technologies). Proteins were transferred onto nitrocellulose membranes using the iBlot system (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... using either XCell SureLock Mini-Cell with XCell II Blot Module or Mini Gel Tank with Blot Module (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... To determine the point of elution of aSyn from the chromatography column protein fractions which were collected and monitored on absorption at 280 nm were ran on a 4-12% Bis-Tris gel (Invitrogen, Thermo Fisher) using SDS-PAGE and stained with Coomassie blue ...
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bioRxiv - Biophysics 2020Quote: ... All samples were then re-suspended in 12% SDS loading buffer and run on a NuPAGE™ 4–12% Bis-Tris gel (Invitrogen) at 200 V for 45 min ...
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bioRxiv - Cancer Biology 2021Quote: ... equal amounts of protein (typically 20-50 µg of protein per lane) were separated by electrophoresis on a NuPAGE 10% Bis-Tris Gel (Invitrogen, NP0315BOX) in NuPAGE™ MOPS SDS Running Buffer (20X ...
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was performed using Invitrogen Bolt 4-12% Bis-Tris Plus Gels (NW04120BOX, NW04122BOX, or NW04125BOX Thermo Fisher Scientific Inc.) in 1× running buffer with 3-(N-morpholino)propanesulfonic acid (MOPS ...
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bioRxiv - Cell Biology 2020Quote: ... Identity and purity of CIZ1-containing elution fractions was assessed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) with SimplyBlue™ safe stain (Invitrogen) and pre-stained protein ladder 10-250 kDa (Thermo Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... were loaded on to NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate 1.0 mm Mini Protein Gels (Invitrogen, NP0321, EA0375PK2) buffer alongside PageRuler Protein Ladder (5 µL/lane ...
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bioRxiv - Cell Biology 2020Quote: ... samples were separated on a Novex™ NuPAGE™ 4-12 % Bis-Tris Mini Protein Gel (Art. No. NP0323, Invitrogen™) in 1x Novex™ NuPAGE™ MOPS SDS Running Buffer (Art ...
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bioRxiv - Cell Biology 2021Quote: 40 µg of brain extracts were loaded onto a NuPAGE 4–12% Bis–Tris Midi Gel (Thermo Fisher Scientific, Cat# WG1402BOX) and electrophoresed at 130 V for 2 hrs with NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins in the gel were then transferred to a membrane using the iBlot 2 membrane transfer device (Invitrogen, IB21001, US) (2014) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with a 4x solution of NuPage LDS sample buffer containing 100 mM DTT and loaded on SDS PAGE gels (Invitrogen, XP04205) and analysed by Coomassie protein stain (Abcam ...
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bioRxiv - Genomics 2020Quote: ... This piece was then recirculized in a 20 µL reaction containing 60 ng of gel purified DNA and 1 µL T4 DNA Ligase (Thermo Fisher) in buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Normalized soluble supernatant or 20 μL of conditioned media was run on a 4-20% Tris-Gly SDS-PAGE gel (Life Technologies) and transferred onto a nitrocellulose membrane using an iBlot2 device (P0 program ...
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bioRxiv - Microbiology 2022Quote: ... Parasite proteins were reduced with 200 □M dithiotreitol (DTT) and separated on 4-12% NuPAGE Bis-Tris gels (Thermo Fisher Scientific). Following wet protein transfer to nitrocellulose ...
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bioRxiv - Neuroscience 2022Quote: ... The footprint samples were size-selected using the 17 and 34 nt markers as a guide on a 15% TBE-Urea polyacrylamide gel (Thermo Scientific), while total RNA samples were randomly heat fragmented by incubating at 95 °C in an alkaline fragmentation solution (50 mM NaCO3 pH 9.2 ...
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bioRxiv - Neuroscience 2022Quote: ... 4 μg of purified protein was mixed with DTT and analysed using a NuPAGE™ 4-12% Bis-Tris mini protein gel (ThermoFisher) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 25 μg total protein extract was fractionated on a 10% polyacrylamide gel and transferred onto PVDF membranes (ThermoFisher Scientific, United States). The membrane was blocked with 5% non-fat dry milk in 0.2% Tween-20 in Tris-buffered saline for 1h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... All purified recombinant mAbs were analyzed by SDS-PAGE under reducing conditions using NuPAGE Novex 4-12% Bis-Tris polyacrylamide gels and NuPAGE reducing agent (Life Technologies) according to the manufacturer’s instructions and by binding to SARS-CoV-2 spike and RBD by ELISA.
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bioRxiv - Biochemistry 2022Quote: ... and heated at 95ºC for 5 minutes and resolved using a 4-20% tris-glycine gel (ThermoFisher Scientific, Waltham MA, XP04200). Next ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μl of each binding reaction was loaded directly onto a pre-run 6% acrylamide DNA retardation gel (Thermo Fisher Scientific) and electrophoresed at ≤20 V cm−1 at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10-20 µg of protein were loaded into 4-12% Bis-Tris Bolt gel and run at 150-200V constant for 1h in Bolt MOPS/MES 1X running buffer (Thermo Scientific). Dry transfer of the proteins to a nitrocellulose membrane was done using IBLOT2 (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was stained in 20 ml of 1 x TBE buffer containing 2 μl of SYBR Gold stain (ThermoFisher Scientific) for 5 minutes at room temperature and a band migrating just above the 35 nt oligo was cut from the Ribo-seq sample lane.
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bioRxiv - Immunology 2022Quote: ... The cell lysate was heated to 95°C for 10 minutes and was resolved by SDS-PAGE using a 4-12% Bolt Bis-Tris gel (Thermo Fisher) and transferred to a nitrocellulose membrane (Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: ... Proteins and DNAs were incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Genetics 2022Quote: ... aliquots of each product were resolved via electrophoresis in agarose gels to confirm amplification of the SVA repeat sequence and then run on the ABI 3730 DNA sequencer (Applied Biosystems) with GeneScan 500 LIZ as internal size standard ...
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bioRxiv - Pathology 2022Quote: ... Amplified PCR products (766 bp) from each fish were isolated using NucleoSpin™ Gel PCR Clean-up Kit (Fisher Scientific, Sweden) according to the manufacturer’s protocol and then submitted for sequencing service (U2Bio ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were centrifuged at 14,000g for 5 minutes at 4°C and electrophoresed on NuPAGE™ 4-12% Bis-Tris Polyacrylamide gels (Thermo Fisher) with NuPAGE™ MOPS running buffer (Thermo Fisher ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were further purified before analysis by MS by loading directly onto a precast 4-16% BN-PAGE gel (Life Technologies).
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bioRxiv - Neuroscience 2022Quote: ... and loaded into a NuPAGE 4–12% Bis-Tris Gel in a chamber filled with NuPAGE™ MOPS SDS running buffer (ThermoFisher) and run at 100V for ∼110 min ...
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bioRxiv - Pathology 2022Quote: ... Protein concentration was determined with Bio-Rad Protein Assay Kit II and 15 μg sample protein was loaded in a NuPAGE 3-8 % Tris-Acetate gel (Thermo Fisher). The gel was run at a constant voltage of 150 V for 4 h ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 50 μg of WCE or 20 μg of nuclear extract was electrophoretically separated on a 4-20% Tris-glycine gel (EC6028; Life Technologies) and transferred to nitrocellulose membranes using an iBlot 2 Transfer Stack (IB 3010-01 ...
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bioRxiv - Physiology 2022Quote: ... Products were then analysed by 1% agarose gel electrophoresis in TBE buffer followed by SYBR Gold staining (catalog no. S11494, ThermoFisher Scientific). Gels were imaged with a ChemiDoc MP Imaging System ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lysates were quantified by bradford and equal amount of total protein was loaded per lane on NuPAGE 4%–12% Bis-Tris pre-cast gels (Life Technologies), transferred to nitrocellulose membranes (Whatman) ...
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bioRxiv - Microbiology 2022Quote: ... The samples were resuspended in hot Laemmli buffer and separated in NuPAGE™ 4 to 12% Bis-Tris protein gels (Invitrogen) before in-gel digestion ...
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bioRxiv - Bioengineering 2022Quote: ... was used to cool down the pancreas before excising and trimming it into smaller blocks to be embedded in 3.8% agarose gel (Invitrogen #15517-022). These blocks were then covered with ice-cold extracellular media and glued on the vibratome (Leica Microsystems ...
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bioRxiv - Microbiology 2022Quote: ... The amplified PCR fragments were purified using a gel/PCR DNA isolation system (Viogene) and sequenced in both directions using the ABI PRISM 3130 genetic analyzer (Applied Biosystems) with specific primers for SARS-CoV-2.
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bioRxiv - Bioengineering 2022Quote: ... Protein was transferred to a nitrocellulose membrane for 7.5 minutes via the iBlot™ 2 Gel Transfer Device (IB21001, Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Purified surface proteins or total cell lysates were separated on a NuPage 3-8 % Tris-acetate protein gel (Thermo Fisher Scientific) at 200 V for 40 min and transferred to a PVDF membrane ...
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bioRxiv - Microbiology 2022Quote: ... The quality and quantity of the retrieved DNA were assessed both before and after treatment via gel electrophoresis and Nanodrop analysis (ThermoFisher Scientific). Sequencing libraries for salivary samples were prepared using the NEBNext Ultra DNA Library Prep kit (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... boiled for 15 min at 100 °C and loaded into the wells of a gel alongside a molecular weight marker (Thermo fisher). The gel was run in a Mini-PROTEAN Tetra Cell system (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... IP control antibody included IgG purified from normal rabbit serum with Melon Gel IgG Spin Purification Kit (Thermo Scientific, Rockford, IL). The Co-IPs performed in triplicate.
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bioRxiv - Biochemistry 2022Quote: ... purified RML or ME7 fibils were prepared as above and subjected to SDS-PAGE separation using NuPAGE 12% Bis-Tris mini protein gels (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... gels were functionalized with 0.5 mg/ml solution of sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino) hexanoate (Sulfo-SANPAH) (Thermo Fisher Scientific) prepared in 50 mM HEPES buffer (Santa Cruz Biotechnologies) ...
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bioRxiv - Biophysics 2022Quote: ... The no-UV monomer and plus-UV dimer bands were excised and were each digested in-gel with MS grade Trypsin (Thermo Scientific) and GluC (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... a total of 120 μg of protein from lysates was migrated on a pre-cast 4-12% Novex NuPAGE SDS Mini Gel (Invitrogen/Thermo Fisher) using the Bis/Tris buffer system (product number NP0326BOX ...
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bioRxiv - Biophysics 2022Quote: ... An aliquot of the full-length construct and the post-reaction purified KcsA were analyzed by SDS-PAGE using a 4–12 % Bis-Tris mini gel (Thermo Fisher) at 200 V for 35 min ...
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bioRxiv - Bioengineering 2022Quote: ... Flash column chromatography was performed using silica gel 60 (40-63 μm, 230-400 mesh, 60 A°) purchased from Fisher Scientific. Analytical thin-layer chromatography (TLC ...
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bioRxiv - Developmental Biology 2022Quote: ... For western blot analysis 40 μg of protein extracts were loaded per well on Bolt 4-12% Bis-Tris Plus Gels (ThermoFisher, NW04120BOX) and blotted on PVDF immobilon-p membranes (Millipore ...