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Citations for Thermo Fisher :
8151 - 8200 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the sensitizing media was then replaced with CO2-independent imaging medium (Phenol Red-free Leibovitz’s L-15 medium (Life Technologies) supplemented with 20% fetal bovine serum ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 picomol/μl of each primer per reaction in 10μl of final volume in ABI 7500 real-time PCR machine (Applied Biosystems). The relative fold change in mRNA expression for different genes was calculated using the comparative CT method to assess changes in gene expression ...
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bioRxiv - Cell Biology 2023Quote: ... For hESC-derived cerebral organoid differentiation hESCs were transferred while splitting on MEF feeder layer in ESC medium (DMEM F12 + L-glutamine (Gibco), 20% KnockOut Serum Replacement (Gibco) ...
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bioRxiv - Bioengineering 2023Quote: ... with Donkey anti-Rabbit IgG (H+L) highly cross-adsorbed secondary antibody alexa fluor 555 (Catalog A-31572, ThermoFisher Scientific) staining of the same spatial locations (Supplemental Fig ...
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bioRxiv - Neuroscience 2023Quote: ... were isolated from embryonic day 18 Sprague-Dawley rat embryos and cultured on poly-L-lysine coated plates or glass coverslips in Neurobasal medium containing N2 and B27 supplements (Invitrogen). Neurons were maintained in a 5% CO2 humidified atmosphere at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... the surfaces were rinsed thrice with PBS and the chambers were incubated with 0.5 g/l BSA-Biotin (ThermoFisher, 29130) in PBS overnight at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... Goat anti-rabbit IgG (H+L) secondary antibody was reconstituted in labeling buffer using 0.5 mL spin-desalting columns (40K MWCO, ThermoFisher, 87766). The conjugated antibody was purified using spin-desalting columns and stored in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... coated #1.5 18 mm glass coverslips in DMEM (4.5 g/L-1 D-G-glucose) supplemented with 10% fetal bovine solution (Gibco™), 1% antibiotic solution (penicillin Streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... cells were harvested and resuspended in ice-cold FACS buffer (2% v/v FBS, 50 mM EDTA in DMEM supplemented with high glucose, L-glutamine and HEPES, without phenol red [Gibco]). Cells were incubated with 5 μg/mL of RBD antibody CC12.325 for 1 h at 4 °C with gentle rocking ...
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bioRxiv - Genomics 2023Quote: ... were grown for at least three days in standard media containing RPMI 1640 + L-Glutamine (Hyclone) supplemented with 10% Fetal Bovine Serum (Gibco) and 1% Penicillin-Streptomycin (Gibco) ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... and the medium was then replaced with serum-free advanced DMEM supplemented with 2 mM L-glutamine and 1% antibiotic-antimycotic (Gibco). After incubation for 48 hours ...
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bioRxiv - Immunology 2024Quote: ... H292 cells were cultured in complete RPMI medium (cRMPI) (RPMI 1640, L-glutamine, 10% heat-inactivated fetal bovine serum [FBS] (Gibco) and 1% penicillin/streptomycin ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed with DMEM and incubated with DMEM either without FBS (L cells) or exosome free 10% FBS (for NHLF, HDFn, BJ, MEFs) (Gibco). After 3-4 days ...
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bioRxiv - Neuroscience 2024Quote: ... L cells were (1 × 106) were transfected with 40 nM siGENOME SMARTpool siRNAs (Dharmacon) using LipofectamineTM RNAiMAX reagent (Invitrogen, 13778075) according to manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... and enzyme activity was quenched by addition of FCS followed complete DMEM medium (supplemented with 10% FCS, 2 mM L-glutamine (ThermoFisher) and 10000 U/mL penicillin/streptomycin (ThermoFisher)) ...
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bioRxiv - Cancer Biology 2024Quote: ... and pooled stably expressing cell lines were selected with and maintained in indicated medium further supplemented with 500 µg/L puromycin (#A11138, Gibco).
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bioRxiv - Cancer Biology 2024Quote: ... coverslips were incubated in Goat anti-Rabbit IgG (H+L) highly cross-adsorbed Alexa Fluor Plus 488-conjugated secondary antibody (Invitrogen) at a concentration of 1:1000 in Background Sniper for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were cultured under stem cell-like conditions in Neurobasal A medium supplemented with 2 mM L-glutamine (GlutaMAX supplement, 35050061; ThermoFisher) 1X B27 (17504044 ...
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bioRxiv - Biochemistry 2024Quote: ... Aliquots of 4 μL of reaction were terminated at the indicated timepoints by addition of 20 μL 1.2x NuPAGE LDS buffer (Thermo Fisher) supplemented with 120 mM DTT ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 488 and goat anti-rabbit IgG (H + L) Alexa Fluor 647 secondary antibodies (1:200 in PBS; Cat. Nos. A11017 and A21245, Invitrogen) for 3 h ...
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bioRxiv - Neuroscience 2024Quote: ... 250,000 hippocampal cells were plated into a 12-well poly-l-lysine-coated plate in Neurobasal Medium (Invitrogen; Waltman, MA) supplemented with 1 μg/ml gentamycin (VWR ...
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bioRxiv - Cancer Biology 2024Quote: ... Collagen plugs were set at 37°C/5%CO2 for 10 minutes, and then fibroblast growth medium was added (DMEM medium supplemented with 10% FCS, 2 mM L-glutamine (ThermoFisher) and 100U/mL penicillin/streptomycin (ThermoFisher)) ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then washed extensively in TBS before being incubated with biotinylated goat anti-rabbit IgG (H+L) secondary antibody (ThermoFisher Cat# B-2770 ...
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bioRxiv - Bioengineering 2024Quote: ... A secondary labeling step was performed with goat anti-mouse IgG (H+L) HRP conjugate according to manufacturer’s instructions (Thermo Fisher) to facilitate chemiluminescent detection ...
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bioRxiv - Bioengineering 2024Quote: ... After incubation with a similarly diluted alkaline phosphatase-conjugated goat anti-mouse IgG (H+L) secondary antibody (31346, Fisher Scientific), and washing steps ...
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bioRxiv - Biochemistry 2024Quote: ... PXDN-over-expressing HEK293T cells and catalase-over-expressing HEK293T cells were cultured in DMEM containing 4.5 g/l glucose (Gibco, 11965). The culture medium was supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... 12.5 μL of 2 × reaction buffer provided with the Superscript III one step RT-PCR system with Platinum Taq Polymerase (Invitrogen, Darmstadt ...
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bioRxiv - Microbiology 2024Quote: One million cells were spun down in 1.5 mL tubes and lysed in 150–200 μL SDS sample buffer (2% SDS, 50 mM Tris, 12.5 mM EDTA, plus Halt protease and phosphatase inhibitors (Thermo Fisher)) ...
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bioRxiv - Physiology 2024Quote: ... were cultured in pen/strep-free media overnight and transfected with 25 nmol/L of either Lgr4 (Thermo Fisher Scientific) or scrambled (SC ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with ice-cold PBS and the primary antibody was detected using FITC-conjugated goat anti-mouse IgG (H+L) antibody (Invitrogen). Relative pre-TCR surface pre-TCR expression at each time point was calculated as the percentage of positive cells normalized to time 0.
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bioRxiv - Immunology 2024Quote: ... Lavages of the same genotype were pooled and resuspended in complete medium (RPMI 1640 supplemented with 10% FBS, 100 U/ml penicillin, 10 μg/ml streptomycin, and 400 μM L-glutamine [Invitrogen]). Typically ...
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bioRxiv - Neuroscience 2024Quote: ... and (2) Alexa Fluor Plus 488 Goat anti-Rabbit IgG (H+L) (for Calretinin stain; Catalog # A32731, Invitrogen; RRID: AB_2633280). Sections were then incubated for 10min in a 1:40,000 dilution of DAPI stock solution in PBT (5mg/mL ...
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bioRxiv - Microbiology 2024Quote: ... antibodies at a concentration of 20 µg/mL followed by goat anti-mouse IgG (H+L) AF488 secondary antibodies (Invitrogen). Data was acquired on a BD FACS Canto II and analyzed using FlowJo v10.7.1.
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bioRxiv - Bioengineering 2024Quote: ... 7–35) were routinely grown as described with high glucose (4.5 g/L) and 1% (v:v) GlutaMax (Thermo Fisher Scientific), supplemented with 10% (v:v ...
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bioRxiv - Plant Biology 2024Quote: ... 0.2-0.5 µl of the remaining PCR product was used directly for sequencing using BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) or SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems).
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bioRxiv - Cell Biology 2024Quote: ... in PBS and 0.l µm Tetraspec beads were added to the Ibidi channels (l:l00 dilution from stock, T7279, Thermo Fisher) to be used as fiducials for drift correction ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used in this study were: anti-rabbit IgG (H+L) Alexa Fluor 488 (Invitrogen, A11008, 1:800 (IF)) ...
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bioRxiv - Cell Biology 2024Quote: ... we conjugated poly-L-lysine/polyethylene glycol to the PA substrate using the bi-functional crosslinker sulfo-SANPAH (ThermoFisher Scientific). We sterilized low-attachment microwell substrates with antibiotics and then seeded a single-cell suspension of MDCK stable lines onto the substrate surface ...
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bioRxiv - Cell Biology 2024Quote: ... 1:500 dilution of Alexa Fluor Plus 647 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (Invitrogen) was added to the coverslips and incubated for 1 hr at room temperature.
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bioRxiv - Immunology 2024Quote: ... Cells were plated in complete (c)RPMI (RPMI supplemented with 10% FBS, 1% sodium pyruvate, 1% HEPES and 1% L-glutamine, all GIBCO) supplemented with Penicillin-Streptomycin (100 U mL-1 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and then washed twice in antibody mix (1:200 FluoTag-X2 anti-ALFA Alexa647 [Nanotag, Cat No. N1502-AF647-L]; 1:200 phalloidin Alexa546 [Invitrogen, Cat ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded in coverslips treated with poly-L-lysine (10 μg/mL) and grown for 6 days in Neurobasal medium (Gibco) supplemented with 0.05% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... The CCE mouse embryonic stem cell (ESC) line (Robertson et al., 1986) was cultured in DMEM supplemented with L-glutamine (Gibco), 15% FBS (Gibco) ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... and after 24-hour the medium was replaced with serum-free advanced DMEM supplemented with 2 mM L-glutamine and 1% antibiotic-antimycotic (Gibco). After incubation for 48 hours ...
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bioRxiv - Cell Biology 2024Quote: ... we used mouse BCAR1 siRNA (12927; ON-TARGETplus, SMARTpool, L-041961-00-0005, Horizon Discovery/ Dharmacon) or scrambled control siRNA (AM4636; Ambion). Knockdown efficiency and p130Cas over-expression levels were confirmed by Western blotting of cell lysates in radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Cell Biology 2024Quote: ... Clarified bacterial lysates from a 1 L culture were bound to 1 mL column of HisPur Ni-NTA Spin column (ThermoFisher) by gravity flow ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used: goat anti-mouse IgG (H + L) highly cross-adsorbed secondary antibody Alexa Fluor 488 (Invitrogen/ThermoFisher), and goat anti-rabbit IgG (H + L ...
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bioRxiv - Neuroscience 2024Quote: Spinal cords were dissected from E11.5 mouse embryos as previously described36 in ice-cold L-15 medium (Gibco, #11415-114), and dorsal regions of the spinal cord were cut into 150 x 150 µm segments ...
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bioRxiv - Neuroscience 2024Quote: ... 15 μL of master mix was added to each sample to a final concentration of 0.8X First Strand Buffer (Invitrogen, 28025013), 0.4 mM dNTP mix (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... at a final concentration of 10 or 20 nmol/L for 72 h using Lipofectamine® RNAiMAX reagent (Invitrogen, 13778-150) in an antibiotic-free EGM-2 medium (without GA-1000) ...