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Citations for Thermo Fisher :
8101 - 8150 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Successful enrichment was validated by running 5 ul sample elution on NuPAGE 4-12% Bis-Tris gels followed by silver stain (ThermoFisher LC6070) following the manufacturer’s protocol.
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bioRxiv - Biophysics 2021Quote: ... The purity was checked by SDS- PAGE gels and molecular weights were verified by ESI-MS and Voyager STR time-of-flight-mass spectrometer (Applied Biosystems). The protein concentration was determined by the UV spectroscopic method in the presence of 8 M urea (44).
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of each sample was separated in 8% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (45-004-110, Fisher Scientific) in ice-cold transfer buffer (25 mM Tris-base ...
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bioRxiv - Neuroscience 2021Quote: ... Cell lysates were prepared as described and equal protein amounts were loaded onto 4% to 12% Bis-Tris NuPAGE gels (ThermoFisher Scientific). After electrophoreses ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were run on a 4%–12% polyacrylamide gel for 1 hour at 150 V and colored with PageBlue Protein staining solution (Thermo Scientific) overnight.
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bioRxiv - Cell Biology 2021Quote: ... 30 µg of protein from each lysate was loaded onto a Bolt 4-12% Bis-Tris Plus SDS gel (Life Technologies) for separation for 75 min at 100 V ...
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bioRxiv - Biophysics 2021Quote: 2% agarose gel was prepared in 1X TBE buffer supplemented with 10 mM MgCl2 and prestained with Sybr safe (Thermofisher, USA). 5 nM 10 ul DNA nanotube was mixed with DNA loading dye (Thermofisher ...
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bioRxiv - Cell Biology 2021Quote: ... or 1 cm 4-12% Bis-Tris gel for ProQ diamond stain with 1x MOPS running buffer (Thermo Fisher Scientific, NP0001), transferred onto a PVDF membrane (EMD Millipore ...
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bioRxiv - Cancer Biology 2020Quote: ... The amplified products were then resolved by electrophoresis using a 1.2% E-gel containing a SYBR Safe stain (Thermo Fisher Scientific), which was visualized on an E-Gel Imager Blue-Light Base (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by peptide analysis of the digested gel bands by C18 reversed-phase chromatography using an UltiMate 3000 RSLCnano System (Thermo Scientific) equipped with an Acclaim PepMap C18 column (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: The multimerization status of full-length and specified truncated SPR2-GFP proteins was analyzed using the NativePAGE Bis-Tris gel system (ThermoFisher Scientific). 2 μM of purified proteins were loaded onto a 4-16% NativePAGE gel and ran according to the manufacturer’s procedure at 150 V constant for about 120 min.
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bioRxiv - Cancer Biology 2020Quote: ... which then were cross-linked to polymerizing PAA gels premixes by their biotin tags to streptavidin-conjugated polyacrylamide (Streptavidin-acrylamide, Thermo Fisher). For that 7-10 μL of PAA premix of desired G’ was polymerized in the “sandwich” fashion between an “intermediate” patterned surface and glass-bottom 35 mm Petri dishes (MatTek Corp. ...
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bioRxiv - Cancer Biology 2020Quote: ... reagents including the SDS-PAGE (4–12% Tris-Glycine) gels and Nitrocellulose membranes of iBlot Dry-blotting system were purchased from Life technologies. Anti-actin (1:100,000 ...
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bioRxiv - Cell Biology 2021Quote: Equal amounts or fractions of indicated protein lysates were loaded onto NuPage 4-12% Bis-Tris Protein Gels (Invitrogen, Waltham, MA), and then subsequently immunoblotted with the primary antibodies listed in Supplementary Table 2 ...
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bioRxiv - Neuroscience 2020Quote: ... A total of 20 to 40µg of protein were loaded per lane and separated by 4-12% Bolt-PAGE gels (Invitrogen catalog # NW04122BOX) and transferred to nitrocellulose membrane in Towbin buffer that contained 25mM Tris ...
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bioRxiv - Pathology 2021Quote: ... Protein concentration was determined by absorption spectroscopy and purity by SDS-PAGE electrophoresis on 4-12% NuPAGE gels in MES buffer (Life Technologies).
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bioRxiv - Plant Biology 2021Quote: ... RNA concentration and purity were determined using Nanodrop and its integrity assessed in a 1% agarose gel stained with SYBR Safe (InvitrogenTM, Life Technologies).
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bioRxiv - Biochemistry 2020Quote: ... Samples were denatured by adding 4xLDS buffer and heating at 70°C for 10 min before being separated in 3-8% Tris-acetate gels (Thermo Fisher) run at 150 V for 3 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Equal amounts of protein (20-50 μg) were loaded and were separated by electrophoresis in 4 to 12% Bis-Tris Gel (ThermoFisher Scientific), transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Cell Biology 2021Quote: ... Samples prepared in NuPAGE LDS Sample Buffer were resolved on a Novex 4-12% Bis-Tris precast gels (Invitrogen, Waltham, MA) and blotted to nitrocellulose using the iBlot semi-dry transfer system (Invitrogen ...
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bioRxiv - Synthetic Biology 2021Quote: ... Samples in the amount 50-100 micrograms (GFP) or 200-400 ug (HA tag) were separated using NuPAGE 4 – 12% precast gels (Life Technologies) in SDS-containing buffer and transferred to Immobilon membrane (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: The samples were alkylated in presence of 120 mM iodoacetamide for 20 min in the dark and run on a precast 4-12% Bolt Bis-Tris plus gel (Thermo Fisher) using 1x MES buffer (Thermo Fisher) ...
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bioRxiv - Plant Biology 2020Quote: ... and a final extension of 5 min at 72°C. The target band (approx. 100 bp) was extracted from a 2% E-Gel (Thermo Fisher). For multiplex sequencing ...
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bioRxiv - Microbiology 2020Quote: ... 100 mM) was included in the loading dye and loaded on a Novex 10-20% Tris-Glycine gel (Thermo Fisher Scientific). Gels were run in a buffer containing 25 mM Tris ...
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bioRxiv - Microbiology 2020Quote: The crude extract was fractionated by vacuum liquid chromatography on silica gel 60 (Acros Organics™ultra-pure 60A 40-63u) eluting with a gradient of n-hexane−ethyl acetate-MeOH to give 10 subfractions (F1-F10) ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR products of ZmHXK7 were purified using Kangwei century Gel Extraction kit (CW23025) and used to construction entry vector (Invitrogen, USA) by TOPO cloning reaction according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants from overnight bacterial cultures were boiled in LDS sample buffer and separated by electrophoresis using NuPAGE 12% polyacrylamide gels (Life Technologies). The contents of the gels were transferred to PVDF membranes ...
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bioRxiv - Microbiology 2020Quote: ... The buffer with dissolved gel slice was added to 1 mL ice-cold 100% ethanol and 2 μL of glycogen (20 mg/ml, ThermoFisher, R0561) and incubated at −20°C for at least one hour ...
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bioRxiv - Microbiology 2021Quote: ... Briefly 2 μg of each protein was diluted in loading buffer and loaded on 10–20% Novex Tricine pre-cast gel (Invitrogen, USA). The run was performed at 120 V for 1 h ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We confirmed the presence of high molecular weight genomic DNA on 1% agarose gels and then quantified sample DNA concentration and purity using a NanoDrop 2000c Spectrophotometer (Thermo Scientific). Samples with < 10 ng/µL of DNA were dried down for 30 min on a vacuum centrifuge and resuspended in ∼50 µL of deionized ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
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Genomic and phenotypic characterization of finger millet indicates a complex diversification historybioRxiv - Genetics 2021Quote: ... The purity and quantity of extracted gDNA was determined by gel electrophoresis and a Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA), respectively ...
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bioRxiv - Microbiology 2021Quote: The plasmid DNA yields from the sewage samples were evaluated using gel electrophoresis and Qubit double-stranded DNA (dsDNA) BR assay kit on a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA). Plasmid DNA purity was measured and validated by absorbance ratio of 260/280 and 260/230 using NanoDrop 100 (ThermoFisher) ...
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bioRxiv - Neuroscience 2020Quote: ... The amplified products were resolved by gel electrophoresis in 2% Roti®agarose (Agarose High Resolution; Roth) in 0.5x UltraPureTM TRIS-Borat-EDTA (TBE) buffer (Thermo Fisher). Non-intron spanning primers for DAZL (DAZL(I)) ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were left at room temperature for 24 hours and run in a 2% agarose gel containing 1/10000 SYBR Gold (ThermoFisher S11494) at 90V for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... The purity of the ATAD2B bromodomain was verified by SDS–PAGE gels stained with GelCode Blue Safe protein stain (Thermo Scientific).
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bioRxiv - Synthetic Biology 2021Quote: ... RNA integrity was confirmed by the presence of 18S and 28S bands on a 2% E-Gel EX (Invitrogen cat# G402002). RNA libraries were prepared with NEBNext Poly(A ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... PCR products were visualized via agarose gel electrophoresis and nucleic acid staining with SYBR safe DNA stain (S33102, Thermo Fisher Scientific), and imaged using an EZ Gel Documentation System (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... All in vitro transcribed RNAs were analysed by electrophoresis in 1.5% formaldehyde agarose gel and RNA concentration was determined on Nanodrop ND-1000 spectrophotometer (ThermoFisher Scientific, USA). RNA was transfected into cells using in-suspension protocol53.
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bioRxiv - Molecular Biology 2020Quote: ... The PCR amplicons were confirmed on a 1% agarose gel electrophoresis and purified with ExoSAP-IT™ PCR Product Cleanup Reagent (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... each ribose-seq library was loaded on a 6% non-denaturing polyacrylamide gel and stained using 1X SYBR Gold (Life Technologies) for 40 min.
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bioRxiv - Microbiology 2020Quote: Viral and cell lysates were resuspended in SDS sample buffer and separated by electrophoresis on Bolt 4-12% Bis-Tris Plus gels (Life Technologies), blotted onto nitrocellulose membranes ...
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bioRxiv - Microbiology 2020Quote: 10 μg per lysate or 10 μL of immunoprecipitated sample was resolved on a NuPAGE 4-12% Bis-Tris Protein Gel (Invitrogen, NP0336). Western blotting was performed using the primary antibodies anti-HA (Proteintech ...
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bioRxiv - Microbiology 2020Quote: ... gel purified and quantified using the Invitrogen™ Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA). For all 2018 samples sequencing was performed using an Illumina MiSeq and the MiSeq Reagent Kit v2 (Illumina ...
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bioRxiv - Microbiology 2020Quote: Eluted proteins were stacked in a single band in the top of a SDS-PAGE gel (4-12% NuPAGE, Life Technologies) and stained with Coomassie blue R-250 before in-gel digestion using modified trypsin (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs and associated proteins were eluted from the beads by boiling for five minutes in 2X SDS-PAGE reducing sample buffer and analyzed on 4-12% Bis-Tris gels (Life Technologies) run in MOPS buffer at 180 V for 60 min ...
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bioRxiv - Neuroscience 2021Quote: ... and samples were loaded onto a BOLT 4–12% Bis-tris gel and transferred using MES buffer (all from Life technologies). Proteins were blotted onto a 0.2 μM nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... Protein samples (10 μg/well) were loaded to NuPAGE Novex 4–12% Bis-Tris protein gel (ThermoFisher Scientific, Carlsbad, CA, NP0336BOX) and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2021Quote: ... The samples intended for HPLC analysis were prepared as follows: the SDS-PAGE gel was stained with colloidal Coomassie Brilliant Blue (Imperial Protein Stain, Thermo Scientific), and the whole lane was cut into four parts and submitted to in-gel digestion ...
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bioRxiv - Cell Biology 2021Quote: ... The T7-gRNA PCR product was gel purified and used as the template for in vitro transcription (MEGA shortscript T7 kit, Invitrogen AM1345). The in vitro transcribed gRNA was then purified using the Invitrogen MEGAclear kit ...