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Citations for Thermo Fisher :
8101 - 8150 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The reaction was stopped with 5 mM L-cysteine and unreacted fluorophore again removed on a Zeba spin column (ThermoFisher). The degree of labeling was determined using the known extinction coefficients of the protein and both fluorophores at their absorbance maxima ...
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bioRxiv - Developmental Biology 2022Quote: ... in 1X phosphate buffered saline with Triton X-100 (PBST) at room temperature for 4 hours and stained with 0.2mg/L DAPI (Life Technologies) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The knock-in A673 cell line expressing endogenous EWS::FLI1-Halo (Chong et al., 2018) was grown in 4.5 g/L DMEM media (ThermoFisher, 10566016) with 10% FBS (Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The pellet of beads is resuspended in 20 μL of bead wash buffer and supplemented by 75 μL of the RT supermix composed of 1.3 x RT buffer supplied with Maxima H Minus reverse transcriptase (ThermoFisher, EP0751), 1.3 mM each dNTP ...
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bioRxiv - Immunology 2023Quote: ... then incubated in wash buffer (Advanced DMEM, 10% FBS, 2 mM L-glutamate, penicillin-streptomycin) with 2 mg/mL collagenase type I (Invitrogen) at 37°C for 5-15 mins with vigorous mixing as previously described.57 Crypts were plated in Matrigel with Human Organoid Growth Media (StemCell) ...
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bioRxiv - Microbiology 2023Quote: ... coli strains were grown overnight in a standard incubator at 37°C with 240 RPM shaking in Lennox L broth (LB, Invitrogen) supplemented when required with appropriate antibiotics (Kanamycin 50 μg/ml ...
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bioRxiv - Evolutionary Biology 2023Quote: Silicic acid uptake was calculated from PDMPO uptake by inoculating 500 μL samples with 0.125 μmol/L PDMPO dye (LysoSensor™ Yellow/Blue DND-160, Invitrogen) for 24 h ...
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bioRxiv - Developmental Biology 2023Quote: Fluor 647 conjugated donkey anti-mouse IgG (H+L) Highly Cross-Adsorbed secondary antibody (Thermo Fisher Scientific, Cat. No. PIA32787), all at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... CaCo-2 CRISPR control and VAPA knock-out cell lines were maintained in culture in DMEM (1x) GlutMax 5g/L D-glucose + pyruvate medium (Gibco) supplemented with 20% fetal bovine serum (Gibco) ...
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bioRxiv - Physiology 2023Quote: ... the slides were washed three times in PBS-T and incubated with goat anti rabbit IgG (H+L) secondary antibody conjugated with Alexa Fluor 568(1:1000, Invitrogen) for 1h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: Parental and SIRT4 wild-type/mutant expressing HEK293 cell lines were cultured at 37°C and 5% CO2 in DMEM (Dulbecco’s Modified Eagle Medium) containing high glucose (4.5 g/L; Thermo Fisher Scientific) with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... highly cross-adsorbed goat polyclonal anti-rabbit IgG (H+L)-Alexa FluorTM Plus 647 (Thermo Fisher Scientific Cat#A32733, RRID:AB_2633282), rabbit anti-PARP clone 46D11 (Cell Signaling Technology Cat#9532S ...
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bioRxiv - Microbiology 2023Quote: ... These saturated YPD cultures were used to inoculate SD minimal medium (6.7 g/L Difco Yeast Nitrogen Base without amino acids but with ammonium sulfate from Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... was reduced on the 78 ± 3 nm cores using 1 ml of 1 mM L-ascorbic acid (AA) (Fisher Scientific), making core@shell structures ...
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bioRxiv - Cancer Biology 2023Quote: ... siLuc (5′-CGUACGCGGAAUACUUCGAUUdTdT-3′) and siARP3 (SMARTpool siRNA L-012077-00-0010 (Dharmacon)) were transfected using RNAiMax (Thermo Fisher Scientific) according to manufacturer’s instruction.
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bioRxiv - Biophysics 2023Quote: ... Cells were then washed six times in PBST after which a Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody AlexaFluor 647 (Invitrogen, A-31571 ...
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bioRxiv - Cell Biology 2023Quote: ... with 1x PBS. After washing, 1 ml of differentiation medium (10% FBS (Millipore, Cat.No. ES-009-B, 1X L-Glutamine (Thermo Fisher, Cat.No.25030081 ...
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bioRxiv - Developmental Biology 2023Quote: ... H2O2 content was measured by incubating lung homogenates with 100 μM/L of Amplex Red and horseradish peroxidase (Invitrogen, A22188) for 30 min at 37 °C ...
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bioRxiv - Biophysics 2023Quote: ... oligonucleotides were dialyzed in ∼1.5 L ultrapure water for 24 h using Slide-A-Lyzer cassettes (2 kDa cutoff, Thermo Scientific), where the water was replaced every ∼6 hrs ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice with PBS and 100 μL of Goat anti-human IgG (H+L) Alexa647 secondary antibody (2 μg/mL, Invitrogen) and Aqua viability dye was added for 20 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: HL-60 cells were grown in RPMI 1640 media supplemented with l-glutamine and 25 mM Hepes (Mediatech) and containing 10% (vol/vol) heat-inactivated fetal bovine serum (Gibco). Cultures were maintained at a density of 0.2–1.0 million cells/ml at 37°C/5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... which were then plated on poly-L-lysine-coated plates in D10C medium (80% high glucose DMEM (Thermo Fisher Scientific), 10% bovine calf serum ...
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bioRxiv - Neuroscience 2023Quote: ... NPCs were thawed onto poly-L-ornithine (PLO) and laminin-coated plates into Neuronal media consisting of Neurobasal Media (Thermofisher), Glutamax (Thermofisher) ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary Antibody (Cat # A10524) and Cy5 conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody (Cat # A10523) were purchased from Invitrogen.
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bioRxiv - Neuroscience 2023Quote: ... Hippocampal neurons were prepared from rat E18 embryos and maintained in Neurobasal Medium supplemented with B-27 and 2 mM L-glutamine (Invitrogen) for 18-21 days in vitro (DIV).
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bioRxiv - Cancer Biology 2023Quote: ... and 2ug/ml of secondary goat anti-rabbit IgG (H+L) antibody conjugated with Alexa Fluor 488 (Invitrogen, A-11008) at RT for 1h ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK-293 (CRL-1573) cells were obtained from ATCC and maintained in high-glucose (4.5 g/L) DMEM (Gibco 11995073) supplemented with 4 mM L-glutamine ...
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bioRxiv - Bioengineering 2023Quote: ... from IDT and 2.5µL of the diluted cDNA (1:5 dilution) in a 10µL reaction with Fast SYBR Green Master Mix (Life Technologies) and a LightCycler® 480 Real-time PCR System (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed with TBS-T 5x times and incubated with secondary antibodies: Goat anti-Rabbit IgG (H+L) Secondary Antibody HRP antibody at 1:1000 (#31460, ThermoFisher) or Anti-Mouse IgG (#NA931 ...
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bioRxiv - Biophysics 2023Quote: ... Samples were further desalted using 3 kDa centrifugal filters (Amicon) or dialyzed in ∼1.5 L ultrapure water for 24 h using Slide-A-Lyzer cassettes (2kDa cutoff, Thermo Scientific). Oligonucleotides were prepared in pH* 6.8 400 mM sodium phosphate buffer (SPB ...
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bioRxiv - Neuroscience 2023Quote: ... or mouse Neuroblastoma 2A cells (ATCC; CCL-131) were cultured in DMEM (high glucose and L-glutamine; Gibco 11965–092), supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Plant Biology 2023Quote: ... This tissue was left to recover for 3 days before transfer to AG medium supplemented with 0.2% sucrose and 10 mg/L hygromycin (#10687010; Invitrogen, USA), by moving the filter paper containing the tissue ...
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bioRxiv - Microbiology 2023Quote: ... High Five insect cells were grown at 27°C in Express Five Serum Fee Media supplemented with 16mM L-glutamine (Gibco) and 1% penicillin-streptomycin.
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor 594 and 647-conjugated (A-21209 and A-32733, Life Technologies Alexa Fluor H+L, Carlsbad, CA, USA) were used as secondary antibodies for 45 min.
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bioRxiv - Neuroscience 2023Quote: ... was used to transfect 0.375 ug of plasmid DNA per mL of Opti-MEM + GlutaMAX (+HEPES + 2.4g/L Sodium Bicarbonate; GIBCO, Life Tech). After 7-16 hours ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 and HeLa cells were maintained in CA media (DMEM + 1% non-essential amino acids + 1% L-Glutamine + 10% FBS heat inactivated + 1.43 mM-β-mercaptoethanol) on 100 mm (Nunc) dishes ...
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bioRxiv - Molecular Biology 2023Quote: ... A 10-µL sample of fragmented RNA was run on the first-dimension gel in 0.5x Novex TBE running buffer (Invitrogen, LC6675) at 30 W for 9 min ...
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bioRxiv - Neuroscience 2023Quote: ... diluted 1:2000 (final concentration 0.25 μg /ml) and goat anti rabbit IgG (H+L) poly-HRP (Thermo Fisher, #32260) secondary antibody diluted 1:2000 (final concentration 0.25 ng/ml ...
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bioRxiv - Genomics 2023Quote: ... and 1:200 (anti-NH2-UBP1) and the dilution used for Alexa 568-conjugated goat anti-rabbit IgG (H+L) was 1:10,000 (Molecular Probes). Coverslips were mounted and photographed using a Nikon Y-FL fluorescence microscope.
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bioRxiv - Microbiology 2023Quote: ... 8 µL of normalized culture were mixed with 200 µL of BHI or TSBG media in a 96-well plate (Nunc™ MicroWell™ 96-Well Microplates ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed for 60 min with TBST at room temperature and then incubated for 2h at room temperature with goat anti-rabbit (H+L) HRP-linked secondary antibody (Invitrogen) or goat anti- mouse HRP-linked secondary antibodies (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... N (75 fmol) and L (25 fmol) proteins and the T7 RNA polymerase (50 fmol) using lipofectamine 3000 (Invitrogen™) for 3 h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... 1x Penicillin-Streptomycin-Glutamine (100 units of penicillin, 100 µg of streptomycin, and 292 µg/ml of L-glutamine; Gibco), and 55 µM β-mercaptoethanol (Gibco)) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µl of cells and phage mix was spotted on an agarose pad (1.2% in MQ of UltraPure™ Agarose, Invitrogen) and directly imaged.
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bioRxiv - Cell Biology 2023Quote: Freshly isolated mouse mammary gland tissue was finely chopped and digested in 2.5 mL of Leibovitz’s L-15 Medium (Thermo Fisher Scientific) supplemented with 3 mg/mL Collagenase A and 1.5 mg/mL Trypsin in a GentleMACSTM Octo dissociator (Miltenyi ...
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bioRxiv - Cancer Biology 2023Quote: ... 1640 and MDA-MB-134-IV in 50:50 composition of DMEM and Leibovitz’s L-15 medium (Invitrogen #11415-114). MCF-7/C4-12 (RRID ...
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bioRxiv - Cancer Biology 2023Quote: ... 300,000 cells were transfected with 50nm ON-TARGETplus Human SMARCA4 siRNA (Dharmacon L-010431-00-0005) or the corresponding siControl (D0012061305) by lipofectamine RNAiMAX (ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Monocytes were differentiated into GM-CSF-polarised (M1) MDMs over seven days in RPMI 1640 media supplemented with 1 mM L-glutamine (Gibco), 2 mM sodium pyruvate ...
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bioRxiv - Cell Biology 2023Quote: ... Shinkai) were grown in high-glucose DMEM with supplements (1% penicillin/streptomycin, 10% FBS, 1% NEAA, 1% L-glutamine [Gibco] ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed three times for 15 minutes with TBS-T and the ChmA-containing membrane was incubated with HRP-conjugated goat anti-rabbit IgG (H + L) secondary antibody (dilution = 1:10,000; Thermo Fisher Scientific Cat# 65-6120 ...