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Citations for Thermo Fisher :
8051 - 8100 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... whereas patient-derived cell lines were cultured as adherent monolayers in ECM gel-coated vessels using RHB-A stem cell culture medium (Life Technologies) supplemented with 20 ng/mL epidermal growth factor (Life Technologies) ...
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bioRxiv - Molecular Biology 2019Quote: ... tails were amplified by PCR using a gene-specific forward primer and the oligo-(dT)-anchor and migration of PCR product performed on 3% UltraPure Agarose 1000 gel (Life Technologies).
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bioRxiv - Bioengineering 2019Quote: ... 100 μL gels were formed containing the appropriate GelNB/PEG/LAP concentrations and either 50 nM Block-iT Oligonucleotide (Life Technologies) alone ...
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bioRxiv - Genomics 2020Quote: ... 1% Triton X-100 and 10% glycerol and resolved on 3–8% pre-cast gradient Tris-Acetate gels (Invitrogen, Waltham, MA). For mouse samples ...
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bioRxiv - Microbiology 2019Quote: ... of proteins (corresponding to around 10 pooled organs) were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris Gel, Life Technologies). Separated proteins were transferred to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Cell Biology 2019Quote: ... and 20-50 ug of protein per sample was separated on 4–12% Bis-Tris gels using MOPS or MES running buffer (Life Technologies). Western blotting was performed by wet transfer method in either NuPage transfer buffer or Tris-glycine transfer buffer (Life Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... Binding reactions were incubated for 30 min at room temperature and resolved on 4-20% native polyacrylamide gel (Thermo Fisher Scientific) running in 1×TBE buffer at 90 V for 1h in 4 °C cool-room ...
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bioRxiv - Biophysics 2019Quote: ... as well as 0.5 μL of 10,000X SYPRO Orange Protein Gel Stain (excitation/emission wavelength, λ = 490/590 nm) (Thermo Fisher Scientific). Temperature was increased at a rate of 1°C per minute from 4°C to 100°C and fluorescence was measured every minute with a qPCR thermocycler in FRET mode (Bio-Rad) ...
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bioRxiv - Genetics 2019Quote: ... 1/10 of the aggregates and 5μg of total protein were resolved on a 4-12% NuPAGE Bis-Tris gel (Thermo Fisher, NP0321BOX) followed by staining with the Colloidal Blue Staining Kit (Thermo Fisher ...
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bioRxiv - Biochemistry 2019Quote: ... For MS analysis proteins were run for 1-2 cm on a 4-12% PAGE (NuPAGE Bis-Tris Precast Gel, Life Technologies) and stained with silver (SilverQuest Silver Stain ...
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bioRxiv - Microbiology 2019Quote: Protein analysis was performed on 4%/10% polyacrylamide gels under reducing conditions (molecular weight marker Cat. Nr. 26616, Thermo Scientific, Lithuania). Immunoblotting was done as described in [73] ...
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A DNA-based optical nanosensor for in vivo imaging of acetylcholine in the peripheral nervous systembioRxiv - Bioengineering 2020Quote: ... Native PAGE was conducted to characterize DNA nanostructures by using 4% TBE gel and 1x TBE buffer at a constant voltage of 200 V (electrophoresis system Thermo Scientific) in an ice water bath ...
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bioRxiv - Neuroscience 2019Quote: ... After the last wash PBS was completely aspirated and proteins were eluted from the beads and samples prepared for loading onto electrophoresis gels by adding 150uL-1X sample buffer (IP buffer 100ul buffer + 35uL of 4X NuPAGE™ LDS Sample Buffer ThermoFisher cat# NP0007 ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were mixed in equal density ratios and then mixture PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific). Equal molar concentrations of PCR products for each sample were pooled together ...
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bioRxiv - Molecular Biology 2019Quote: ... The ligation product was purified using Nucleospin Gel and PCR cleanup kit (Macharey Nagel) and desalted using Illustra Microspin G-25 Columns (Thermo Fisher). The purified and desalted ligation product was then electroporated into high efficiency 5-alpha E.coli cells (NEB) ...
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bioRxiv - Biochemistry 2019Quote: ... purity and integrity of the RNA were evaluated by agarose gel electrophoresis and on a ND-2000 spectrophotometer (Thermo Scientific, USA). For each RNA sample ...
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bioRxiv - Genomics 2020Quote: ... The integrity and concentration of RNA was analysed by agarose gel electrophoresis and using the Nanodrop ND-2000 spectrophotometer (Thermo Scientific), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... 50 μg of enriched mitochondria were resolved approximately 6 cm into a pre-cast 4–12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific). The lane was excised and cut in 8 approximately equal chunks and the proteins reduced ...
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bioRxiv - Cell Biology 2019Quote: ... Samples with equal protein amounts were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using standard methods (89) and electroblotted onto polyvinylidene fluoride (PVDF) membrane (ThermoFisher, 88518). After blocking in Tris-buffered saline with 0.1% Tween (TBST ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA quality of each sample was evaluated on an agarose gel and quantified on a Qubit Fluorometer 2.0 (Thermo Fisher Scientific). Double-digest restriction-site associated DNA sequencing (ddRAD ...
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bioRxiv - Bioengineering 2019Quote: ... Proteins were loaded on 4-20%Tris-Gly gradient gels (invitrogen XP04200BOX) then transferred to nitrocellulose membranes with a dry transfer system (Invitrogen iBIot Blotting System and Gel Transfer Stacks Invitrogen IB301001). Membranes were stained with anti-laminin (Sigma L9393) ...
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bioRxiv - Molecular Biology 2019Quote: ... The precipitated and radiolabeled protein-RNA complexes on beads were heat-denatured and loaded on a 4-12% NuPAGE Bis-Tris gel (ThermoFisher NP0321BOX) with 1X MOPS running buffer (ThermoFisher NP0001) ...
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bioRxiv - Evolutionary Biology 2019Quote: Proteins extracted from purified VLPs were using Laemmli buffer were stacked in the top of a SDS-PAGE gel (4-12% NuPAGE, Life Technologies), stained with Coomassie blue R-250 and in-gel digested using modified trypsin (Promega ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were resolved for 4 hr at 100 V on 4% −8% polyacrylamide gradient Tris-acetate denaturing gels (Novex, Life Technologies) and transferred to PVDF membranes ...
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bioRxiv - Plant Biology 2019Quote: ... Mu-STOP transposon was then released from pGEM::Mu-STOP by BglII digestion and purified by GeneJET Gel Extraction Kit (Thermo Fisher). 100 ng of the purified Mu-STOP transposon was mixed with 500 ng of the target plasmid ...
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bioRxiv - Immunology 2019Quote: ... 30 or 40 µg of total protein from tissue lystate was denatured and separated by SDS polyacrylamide gel electrophoresis and transferred to a PVDF membrane (IB401002, Life Technologies). Membranes were cut along the 55 kDa ladder marker and blocked with either 5% BSA or 5% dry skim milk in TBST for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lysates were mixed with 6X sample buffer containing β-ME and separated by SDS-PAGE using NuPAGE 4-12% Bis-Tris gels (Invitrogen), followed by transfer to 0.45µm Nitrocellulose membranes (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... The percentages of acrylamide and bis-acrylamide were adjusted to modify the stiffness of the gel (Hind et al 2015, Reinhart-King et al 2003) and 500nm FluoSpheres (Life Technologies) were added as position markers ...
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bioRxiv - Genetics 2020Quote: ... Products were separated on a 1% SYBR Safe agarose gel and visualized by Safe Imager™ 2.0 Blue Light Transilluminator (Thermo Fisher).
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bioRxiv - Genetics 2019Quote: ... The protein samples were then size-separated by electrophoresis on a gradient Bolt 4-12% Bis-Tris plus gel (Invitrogen NW04120BOX), transferred on a polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA integrity was assessed using 1% (w/v) agarose gel electrophoresis and was quantified using a NanoDrop ND-1000 (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... Samples were then heated at 70°C for 10 minutes before loading on a gradient-PAGE (4 % −12 %) Bis-Tris gel (Invitrogen #NW04125BOX). After their separation ...
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bioRxiv - Genomics 2021Quote: ... gDNA linearization was verified by separation on agarose gels and DNA concentration measured on a Qubit Fluorometer using the Qubit dsDNA HS assay (Thermo Fisher). Linearized gDNA was brought to 2 pg/µL by serial dilution ...
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bioRxiv - Genomics 2020Quote: ... Bands corresponding to digested library vector and insert were cut and products were extracted using GeneJet Gel Extraction Kit (Thermo Scientific) and eluted in 40μl water ...
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bioRxiv - Genomics 2020Quote: ... equal amounts of total protein (10 – 30 μg) were loaded per lane into 4-12% Bis-Tris Bolt gels (Invitrogen # NW04125BOX). Western transfer was performed using an Invitrogen iBlot system (# IB21001 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The quality of the DNA was checked on 1% agarose gel and using a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... The proteins were mixed with sample buffer and G250 loading dye and added to a 4-12% Bis-Tris NativePAGETM gel (Life Technologies). BN-PAGE gels were run for 2 to 2.5 h at 150 V using NativePAGETM running buffer (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted in 6X Laemmli sample buffer and resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Thermo Scientific). Blots were probed with the indicated primary antibodies and with horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2021Quote: ... were then heated at 95° C for 10 minutes before resolving them in 10-20% SDS-PAGE gels (Novex, Life Technologies) and then transferred onto nitrocellulose membranes (VWR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We assessed DNA quality by 0.8 % agarose gel electrophoresis and quantity by fluorometry using a Qubit 4.0 Fluorometer (Invitrogen, Carlsbad, CA, USA). Samples were individually barcoded and pooled into a single library with the NEB Next Ultra DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... Evaluation of protein expression was performed by running 20 μg of total protein lysate on NuPage Bis Tris 4%-12% gels (Invitrogen, USA). Proteins were transferred using iBlot dry transfer system (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were analyzed by SDS-PAGE under reducing conditions (5% β-mercaptoethanol) on 4-12% Bis-Tris NuPage Gels (Thermo Fisher) and cleavage products were detected using mouse monoclonal BC-3 antibody which detects aggrecan cleavage at the Glu392↓Ala393 bond (Cat n. ...
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bioRxiv - Bioengineering 2021Quote: ... These proteins were then reduced at 90°C for 10 min and loaded into a 4-12% bis-tris gel (Life Technologies). This gel was run for 5 min at 150 V in 1x MOPS buffer and stained using Coomassie blue (Bio-Rad ...
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bioRxiv - Bioengineering 2021Quote: ... Cell viability was measured by casting assemblies in wells at initial timepoints or by sectioning gels with a scalpel and staining with Hoechst 33342 (5 µg/ml, stains all cell nuclei, Fisher Scientific) and ethidium homodimer-1 (4 µM ...
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bioRxiv - Biochemistry 2021Quote: Eluted proteins were stacked in a single band in the top of a SDS-PAGE gel (4-12% NuPAGE, Life Technologies) and stained with Coomassie blue R-250 before in-gel digestion using modified trypsin (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... 5 % of the input and all of the output volume were loaded on an SDS-PAGE using 8-12 % Novex Wedge Well Tris-Glycine gel (Thermo Scientific). The gel was then imaged on Amersham Typhoon (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The purity of the BRPF1 bromodomain was verified by SDS-polyacrylamide gel electrophoresis stained with GelCode Blue Safe protein stain (Thermo Scientific).
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bioRxiv - Biochemistry 2020Quote: Proteins extracted from pull-down eluates were stacked in the top of a SDS-PAGE gel (NuPAGE 4-12%, ThermoFisher Scientific), stained with Coomassie blue (R250 ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg protein/lane was then separated under denaturing conditions on reducing 3~8% Tris-Acetate gels (NuPAGE, Thermo Fisher Scientific), and HiMark protein standard (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The purity was checked by SDS-PAGE gels and molecular weights were verified by ESI-MS and Voyager STR time-of-flight-mass spectrometer (Applied Biosystems). The protein concentration was determined by the UV spectroscopic method in the presence of 8 M urea (46).