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Citations for Thermo Fisher :
8051 - 8100 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with plasmids expressing bRSV N-GFP and P in DMEM supplemented with 2% FCS.16 h post transfection medium was replaced with Leibovitz’s L-15 medium without phenol red (Gibco, ThermoFisher Scientific), and imaged live with a Leica TCS SP8 confocal microscope using a 63× oil immersion objective ...
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bioRxiv - Developmental Biology 2022Quote: ... 5% CO2-95% air in Dulbecco’s minimal essential medium (DMEM) containing glucose (4.5 g/l) (Thermo Fisher Scientific, 11965-084) with 10% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated in culture medium that consists of Neurobasal medium supplemented with B27 (1X), L-glutamine (2 mM), and antibiotics (penicillin 200 units/ml, streptomycin, 200 μg/ml) (Invitrogen) for up to 4 weeks at 37 °C in a 5% CO2 humidified incubator ...
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bioRxiv - Microbiology 2023Quote: ... DSM 32851 (L. paracasei) freshly thawed from glycerol stocks stored at −80°C and the live cells diluted in PBS (Gibco). After twelve weeks ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated in culture medium that consists of Neurobasal medium supplemented with B27 (1X), L-glutamine (2 mM), and antibiotics (penicillin 200 units/ml, streptomycin, 200 µg/ml) (Invitrogen) for up to 4 weeks at 37 °C in a 5% CO2 humidified incubator ...
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bioRxiv - Neuroscience 2023Quote: ... explants were seeded in 96-well plates coated with poly-L-lysine/collagen type I and neurobasal medium supplemented with of 1X B27 (50X Gibco), 20 μM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... Lysed cells were resuspended in 500µL of Sonication buffer (SDS 1%, 10mM EDTA, 50mM Tris HCl pH 8) with an anti-protease cocktail (A32965, ThermoFisher) and sonicated for 15” at 4°C (30s ON ...
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bioRxiv - Microbiology 2023Quote: ... Each sample was blocked with 3% BSA in PBS then stained anti-β (1,3)-glucan antibody (Biosupplies Australia Pty Ltd., Australia) followed by secondary antibody of anti-Mouse IgG (H+L) Alexa Fluor 488 (ThermoFisher). The level of β-1,3-glucan exposure on the cell surface was then quantified on a Beckman Coulter CytoFlex 3-laser cytometers ...
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bioRxiv - Cell Biology 2023Quote: ... The pENTR constructs were shuttled into modified pQCXIH-GW-GFP or pQCXIH- GW-Halo plasmids using the L/R reaction of the GatewayTM Cloning system (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then washed and probed with horseradish-peroxidase-conjugated donkey anti-mouse or anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (SA1-100, Invitrogen; 31438, Invitrogen) for 1h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorophore-conjugated secondary antibodies and dilutions used were Alexa Fluor 488 goat anti-rabbit IgG (H + L; 1:500; A-11008, Invitrogen), Alexa Fluor 647 goat anti-rabbit IgG (H + L ...
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bioRxiv - Molecular Biology 2022Quote: 4 L exponentially growing Sf9 cells at a density of 2-3 × 106 cells/mL in Sf900-III SFM media (Invitrogen) was infected with the high-titer baculovirus stock ...
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bioRxiv - Cell Biology 2023Quote: ... Neurons were grown overnight in a plating medium (5% FBS, insulin, glutamate, G5, and B27) supplemented with 100× L-glutamine in Neurobasal medium (Invitrogen). Starting at DIV2 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in 5% FBS in PBS for 1 h at RT with appropriate secondary antibodies (1:2,000): cross-adsorbed goat anti–rabbit IgG (H+L) Alexa 594 (Invitrogen, A11012), anti–mouse IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: ... A total of 8.5 µL extracted RNA was subjected to one-step RT-PCR using the Superscript III one-step RT-PCR system (Invitrogen). The one-step RT-PCR was performed using the forward primer Pol F1 5’-TACAGTGCAGGGGAAAGAATA-3’ (nt 4809-4829 ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with Alexa Fluor™ 568 goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (Invitrogen, A11036) for 3 hours at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... and the following day 50 nM siRNA for DUXA (L-034920-02-0005) or non-targeted control (D-001810-01-05) (SMARTpool, Dharmacon) were transfected using Lipofectamine RNAiMAX (Invitrogen). RNA was isolated 48 hours post-transfection and the effect on cell viability was analyzed at 72 and 96 hours post-transfection.
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bioRxiv - Microbiology 2022Quote: ... Anti-HDAg was detected using donkey anti-rabbit IgG (H+L) Alexa Fluor® 488 conjugate (1:500; Invitrogen, A21206), and Anti-AQP5 ...
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bioRxiv - Microbiology 2022Quote: ... except that the fragment analysis was performed using the ABI3700×L Genetic Analyzer platform (Applied Biosystems, Palo Alto, CA, USA) (14) ...
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bioRxiv - Neuroscience 2023Quote: ... The attached cells were collected using trypsin and cultured on poly(L-lysine)-coated coverslips in proliferation medium containing Dulbecco’s modified Eagle’s medium (DMEM, catalog no. 41965; Thermo Fisher Scientific), Sato Supplement ...
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bioRxiv - Molecular Biology 2023Quote: ... ON-TARGETplus PAFAH1B1 siRNA J- 010330-07-0002 (Dharmacon) or SMARTPool: ON-TARGETplus DYNC1H1 siRNA L-006828-00-0005 (Dharmacon) using Lipofectamine RNAiMax (ThermoFisher). Electroporations were performed 48 hours after siRNA transfection using the Neon Transfection System (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation with goat anti-rat IgG (H+L) secondary antibody tagged with Alexa Fluor 488 (Invitrogen, A-21470). Tissue sections were washed and blocked with 1:100 mouse Fc block in 5% goat serum ...
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bioRxiv - Developmental Biology 2023Quote: ... three OE and three control sibling sticklebacks were euthanized in 250 mg/L MS-222 and their VTPs were collected and placed into 50 µl of Trizol (Invitrogen). Samples were shaken by hand ...
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bioRxiv - Cell Biology 2023Quote: ... were then seeded at a density of ~8 × 104 cells/cm2 in EpiLife medium containing 60 µmol/L CaCl2 (#MEPI500CA Gibco) and 1% Human Keratinocyte Growth Supplement (#S0015 Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μl of eluted proteins were briefly run on SDS-PAGE (4 min at 160 V, 10% SDS gel [NP0301, Invitrogen]), stained with SimplyBlue SafeStain (LC6065 ...
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bioRxiv - Biophysics 2023Quote: ... PLL with 0.1 mg/L were incubated on the surface for 30 minutes and washed with 0.1 M HEPES (Gibco ™, USA) buffer ...
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bioRxiv - Molecular Biology 2023Quote: Vero E6 cells (ECACC batch #15A033) were grown in DMEM (with 10% FBS, 1% L-glutamine, 1% penicillin+streptomycin, no glutamax; Gibco) at 37?C under 5% CO2 ...
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bioRxiv - Genetics 2023Quote: ... in Leibovitz L-15 media (Biowest, Minneapolis, MN, USA) amended with fetal bovine serum bovine (10%) and 1% penicillin/streptomycin (Gibco) and incubated at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... A549 cells were maintained in A549 growth media (DMEM, high glucose, with L-glutamine, without Na pyruvate (Invitrogen 11965-092), with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 μL cell-RNP mixture was aspirated into a 10 μL Neon tip and electroporated by a Neon Transfection System (Invitrogen). YUMM1.7 cells were electroporated at 1500 V ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated on poly-L-lysine coated glass coverslips and transfected using Lipofectamine 2000 or 3000 (Thermo Fisher Scientific). For all transfections ...
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bioRxiv - Physiology 2023Quote: ... and clinical CRS-isolates (Drs. N. Cohen and L. Chandler, Philadelphia VA Medical Center [56]) were grown in Luria broth (Gibco). For anti-bacterial assays ...
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bioRxiv - Cell Biology 2023Quote: ... the ON-TARGETplus human Myo10 siRNA-SMARTpool from Dharmacon RNA Technologies (L-007217-00) was transfected into WT Hela cells using Lipofectamine RNAiMAX reagent (Invitrogen). To increase knockdown efficiency ...
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bioRxiv - Genetics 2023Quote: ... followed by 3 washes with PBST (3% BSA in PBS with 0.1% Triton) and a secondary Alexa Fluor 647 donkey anti-rabbit IgG (H+L) (A31573, Thermo Fisher) for another 30 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... Two μl of the sample was resolved on a PepMap RSLC C18 column (75 μm i.d. x 50 cm; Thermo Scientific) at the flow-rate of 300 nl/min using 0.1% formic acid/acetonitrile gradient system (2-22% acetonitrile in 150 min;22-32% acetonitrile in 40 min ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were grown on 10 cm tissue culture dishes in RPMI 1640 medium with L-glutamine (Thermo Fisher, Waltham, MA) and supplemented with 10% fetal bovine serum (ATCC ...
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bioRxiv - Neuroscience 2023Quote: ... Cell suspensions of mixed neuronal and astrocyte populations were plated onto glass coverslips pre-coated with poly-L-lysine in Dulbecco’s Modified Eagle Media (ThermoFisher Scientific) supplemented with 5% FBS (to promote astrocyte proliferation and to kill neurons) ...
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bioRxiv - Microbiology 2023Quote: ... Then, conidiospores were collected and transferred to a flask with 100 mL potato dextrose broth (PDB, 34g/L) (Thermo Scientific™ Oxoid™ ...
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bioRxiv - Immunology 2023Quote: ... was obtained from ATCC and maintained in RPMI-1640 medium with L-Glutamine (Quality Biological) supplemented with 10% FBS (Gemini Bio- Products) and 1% GlutaMax (Gibco), in vented T25 flasks (CytoOne ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed and blocked with 200 μl of complete RPMI 1640 (10% FBS, 1% Penn-Strep, 1% L-glutamine; Gibco) medium ...
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bioRxiv - Microbiology 2023Quote: Whole bodies from both superinfected and singly infected control groups were collected 3 days post superinfection and placed into 2 ml microcentrifuge tubes containing 300 µl mosquito diluent (20% FBS, 100 µg/ml of streptomycin, 100 µg /ml of penicillin, 50 µg/ml gentamicin [Gibco/Thermo Fisher Scientific] ...
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bioRxiv - Molecular Biology 2023Quote: Transient transfection of siTOP1 (Dharmacon; SMARTpool siRNA, L-005278-00-0005) and siSc (Dharmacon; D-00181010) was achieved using lipofectamine (ThermoFisher). Cells were seeded to reach a confluency of 60-70% on the day of transfection ...
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bioRxiv - Bioengineering 2023Quote: ... The AF tissues were maintained in 4.5-g/L glucose Dulbecco’s Modified Eagle Medium (DMEM, 52100-021, Gibco, Paisley, UK), 10% fetal calf serum (FCS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We diluted the DNA in 50 μl of elution buffer and assessed the DNA concentration of each of the 76 extracts using a Qubit fluorometer (ThermoFisher).
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bioRxiv - Bioengineering 2023Quote: Human alveolar epithelial cells (A549; Cat#: CCL-185, ATCC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) with L-glutamine (Life Technologies) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2023Quote: ... NF-L primary antibody was detected with a Alexa Fluor Plus 647-conjugated goat anti-rabbit IgG (H+L) secondary antibody (A32733, Invitrogen). Tissues were counterstained with DAPI and whole slide images were obtained using a Vectra Polaris slide scanner with a 40x objective (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... or goat anti-Rabbit IgG (H+L) cross-adsorbed secondary antibody Alexa fluor 633 (Thermo fisher, A21070, 1:500 dilution) for 1h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation for 2 hours in secondary antibody (Alexa Fluor 633 goat anti-rabbit IgG (H+L) (Invitrogen A21071) 1:250 in block solution) ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were resuspended in 24.5 ml/tube of complete Percoll media (composed of 25 ml of L-15 medium [Life Technologies] supplemented with 0.429 g/L HEPES [Sigma] ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK293T/17 cells (ATCC CRL-11268) were maintained in DMEM (L-glutamine, Sodium Pyruvate, Non-essential amino acids; Gibco) supplemented with 10% FBS (Gibco ...