Labshake search
Citations for Thermo Fisher :
8001 - 8050 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... and IFT46 mNeonGreen:VashL were boiled in 1X Laemmli Buffer and run in equivalents on 4-15% continuous gradient SDS-PAGE gels (Invitrogen). Bands were visualized by Coomassie staining and normalized between samples ...
-
bioRxiv - Developmental Biology 2024Quote: ... Genomic DNA quality was evaluated by gel electrophoresis and quantity was determined using the PicoGreen DNA quantification assays (Life Technologies). Analysis of the DNA was performed using Illumina Infinium Methylation EPIC v1 BeadChips ...
-
bioRxiv - Cell Biology 2024Quote: ... was used to measure protein concentration of each lysate and 50 μg of total protein of each was run on precast Bis-Tris 4–12% gradient gels (Invitrogen) and protein subsequently transferred to polyvinylidene fluoride membrane (Amersham) ...
-
bioRxiv - Plant Biology 2020Quote: ... and 1 μL purified GST-FDL1 protein were incubated at room temperature for 20 min and loaded on a 6% DNA retardation gel (Life Technologies) before transfer to a nylon membrane ...
-
bioRxiv - Plant Biology 2019Quote: ... The amplified PCR products were resolved on 1.2% agarose gels stained with FloroSafe DNA Stain (Axil Scientific Pte Ltd, Singapore) by using 1kb DNA ladder (Fermentas, Thermo Scientific) as a reference ...
-
bioRxiv - Plant Biology 2019Quote: ... The supernatant was separated on Bolt pre-mixed cast gradient gels 4-12% with Bolt Mes-SDS running buffer (ThermoFisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2019Quote: ... 2x Tris-Glycin buffer was added and 20 µl of sample were resolved by 10% Novex Tris-Glycin gel (Life Technologies). For SDS PAGE ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples were incubated at 70°C for 10 minutes and run on pre-cast NuPage SDS 4-12% gradient gels in NuPage MES Running Buffer (Cat# NP0002, Thermo Fisher). Gels were transferred to nitrocellulose membranes using an iBlot Dry Blotting System (Cat# IB21001 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and protein lysates were subjected to electrophoresis through SDS–PAGE or 4-12% NuPage Bis-Tris protein gels (Thermo Fisher Scientific) followed by blotting onto nitrocellulose membranes ...
-
bioRxiv - Cell Biology 2020Quote: ... the streptavidin pulldown samples were separated by SDS-PAGE on a 4-12% precast gel (Blot™ Bis-Tris Plus, Invitrogen) and then stained with InstantBlue (Expedeon) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Then proteins were separated by electrophoresis in 4%–12% Bis-Tris Protein Gels and transferred to polyvinylidene difluoride membrane (Thermo Scientific). The membranes were blocked in 5% dry milk in 0.1% Tween-20 in PBS and detected with the indicated antibodies ...
-
bioRxiv - Cell Biology 2020Quote: ... and 10 µg of total protein was loaded into NuPAGE™ 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific, #NP0321BOX). Proteins were transferred to a nitrocellulose membrane using the Trans-Blot® Turbo™ RTA Mini Nitrocellulose Transfer Kit and Trans-Blot© Turbo™ Transfer System (Bio-Rad) ...
-
bioRxiv - Cell Biology 2020Quote: ... BMI1 level was assessed by western blot using standard SDS-PAGE in 4%-12% gel of NuPAGE electrophoresis system (Life Technology, Thermo Fisher). Antibodies are ...
-
bioRxiv - Cell Biology 2020Quote: ... H3K27me3 and H2AK119Ub modified histones were analyzed by standard SDS-PAGE in 4%-12% gel of NuPAGE electrophoresis system (Life Technology, Thermo Fisher). Antibodies are ...
-
bioRxiv - Cell Biology 2020Quote: ... the protein equivalent of 300 000 cells for each sample was loaded on NuPAGE Bis-Tris 4–12% acrylamide gels (Life Technologies). Electrophoretic migration was controlled to allow each protein sample to be split into six gel bands ...
-
bioRxiv - Immunology 2021Quote: ... The reactions were incubated for 30 min in the dark after which luminescence was visualized by illumination with 365 nm UV light or on an iBright gel documentation instrument (Thermo Fisher).
-
bioRxiv - Immunology 2021Quote: ... was added to a concentration of 1X and 12.5-30 μg of total protein from each sample was resolved on NuPAGETM 4-12% BisTris (Phospho-PMK-1 and Total-PMK-1) or NuPAGETM 3-8% TrisAcetate (TIR-1::3xFLAG) gels (ThermoFisher Scientific), transferred to nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
-
bioRxiv - Genetics 2021Quote: ... Approximately 50 μg of lysate were electrophetically resolved on 4-20% Novex Tris-glycine gradient gels (Cat# XV04200PK20; ThermoFisher; Waltham, MA). Proteins were then transferred to 0.2 μm PVDF membranes (Cat# 88520 ...
-
bioRxiv - Genetics 2019Quote: ... Supernatants were subjected to preclearing with G sepharose beads prior immunoprecipitation reaction using an overnight incubation with anti-Myc affinity gel (Thermo Scientific). After immunoprecipitation ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples of SMG6-3xFLAG in vitro translation reactions were run on 3-8% Tris-Acetate NuPAGE gel (Invitrogen, Cat. No EA03752BOX). Transfer onto nitrocellulose membranes was performed using the iBlot 2 Dry Blotting System (Invitrogen ...
-
bioRxiv - Developmental Biology 2021Quote: ... The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain, Thermo Scientific). Each lane from the gel was cut and placed in 1.5 ml tubes ...
-
bioRxiv - Genetics 2020Quote: ... The fragment sizes were estimated by running the amplicons on a gel adjacent to a DNA ladder (FastRulerTM Middle Range DNA Ladder, ThermoFisher Scientific).
-
bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were checked on a 1% agarose gel to ensure proper amplification and then purified using Exosap-It (Applied Biosystems) according to manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... The bands were excised from the gel and subjected to a mass spectrometric analysis with Orbitrap Velos Pro (Thermo Fisher Scientific). For protein identification ...
-
bioRxiv - Genomics 2022Quote: ... The obtained Gel Beads-in-emulsion was subjected to reverse transcription using a ProFlex PCR System (Thermo Fisher, Waltham, MA, USA). The resulting cDNA was purified and amplified ...
-
bioRxiv - Microbiology 2022Quote: ... Ligated reaction mixture was heated to 95°C for 10 min for inactivation and mixed with 1 volume of 2x formamide orange G dye before purifying with 10% denaturing urea-TBE acrylamide gel (Thermo Scientific) at 300V for 20 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... the eluted co-precipitated proteins were separated by gel electrophoresis and gel fragments were processed as described in (Tucci et al. 2018) and then measured on a LTQ Orbitrap XL (Thermo Scientific) instrument ...
-
bioRxiv - Cell Biology 2022Quote: ... samples (equivalent of 2 x 105 cells) were subjected to electrophoresis in NuPAGE Novex 4–12% Bis-Tris pre-cast gels (NP0321 from Life Technologies). The same number of cells (see FACS sorting of diploid and tetraploid cells section ...
-
bioRxiv - Neuroscience 2021Quote: ... We loaded 30ug of protein per well into gradient 4-12% NuPAGE Bis-Tris acryl amid gels (#NP0321, Thermo Fisher Scientific) and performed western blotting analysis.Western blotting ...
-
bioRxiv - Molecular Biology 2021Quote: ... and then 10 μg of protein per sample was mixed with SDS and reducing reagent and loaded onto NuPAGE Bis-Tris protein gels (Invitrogen, NP0322) after boiling at 70 °C for 10 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4°C) and boiled at 95°C for 10 minutes before loading on to NuPAGE 4-12% Bis-Tris gels (Thermo Fisher).
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were separated on 4–12% NuPAGE® Bis-Tris gradient gels (1.0 mm x 12 well) (Invitrogen, Cat n°NP0321) using MOPS SDS Running Buffer (Invitrogen ...
-
bioRxiv - Molecular Biology 2020Quote: ... and the desired 200-800 bp DNA library fragments were isolated using PureLink™ Quick Gel Extraction Kit (Invitrogen, cat #K210012). For the clonal barcoding library ...
-
bioRxiv - Molecular Biology 2021Quote: ... supplemented with DTT and boiled at 95°C for five minutes prior to loading onto a BOLT 4-12% Bis-Tris Plus gel (Life Technologies). RNA extraction followed the TRIzol LS RNA extraction protocol (Invitrogen) ...
-
bioRxiv - Molecular Biology 2021Quote: Eluted proteins were separated on a 1.5 mm NuPAGE™ Novex™ 4-12 % Bis-Tris protein gel (Thermo Fisher Scientific) until the running front had migrated roughly 2 cm ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were denatured for 10 min at 70°C while shaking and loaded on 4-12% Bis-Tris gel along with a pre-stained size marker (Thermo Scientific) and electrophoresed at 200 V for 5 h ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 10-20 µg of protein loaded on a 3%–8% NuPAGE Tris-Acetate Gel in tris-acetate buffer (Novex Life Technologies) or a 4-12% NuPAGE TrisBis NuPAGE gels in MES buffer (Novex Life Technologies ...
-
bioRxiv - Molecular Biology 2020Quote: ... The samples were then denatured for 5 min at 95C and loaded onto a 4-12% Novex Bis-Tris acrylamide gel with 1x MES running buffer (Life Technologies) run at 120V for 1.5-2h ...
-
bioRxiv - Biophysics 2022Quote: Samples were heated at 95°C for 5 minutes before loading the onto the PAA-gel (marker: PageRuler Plus Prestained protein ladder (Thermo Scientific)) ...
-
bioRxiv - Immunology 2021Quote: ... and checked for purity and quality on a silver-stained SDS-PAGE gel (3-8% Tris-Acetate Novex, Thermo Fisher Scientific). The anti-NTD neutralizing antibody NTD-18 and the pan-coronavirus anti-S2 non-neutralizing antibody Ab-10 were previously described 9,10.
-
bioRxiv - Genomics 2020Quote: ... samples were heated for 5min at 70°C and size-separated by electrophoresis using 1.5mm 4-12% NuPAGE Bis-Tris protein gels and 1x NuPAGE MES SDS running buffer (Thermo Fisher Scientific) for 35-50min at 200V ...
-
bioRxiv - Molecular Biology 2021Quote: ... The eluted proteins were analyzed on a NuPAGE 4-12% Bis-Tris 1.0 mm Mini Protein Gel (Invitrogen, cat. No. NP0321) and stained with Instant Blue (Abcam ...
-
bioRxiv - Molecular Biology 2021Quote: ... was denatured at 70°C for 5 min and resolved on 4-20% Tris-borate-EDTA (TBE) Urea gel (ThermoFisher scientific) in cold 0.5 × TBE buffer (Invitrogen ...
-
bioRxiv - Molecular Biology 2021Quote: ... the gels were fixed by 40% ethanol/10% acetic acid for 1 hr and stained overnight in 200 mL of Krypton Fluorescent gel stain (Life Technologies) diluted 1/10 in water ...
-
bioRxiv - Biophysics 2019Quote: ... S4) and mixed with 15 μl of denaturing buffer containing 5 μl 4x Tris/SDS NuPAGE gel-loading buffer (Thermo Fisher), 10 mM tricarboxyethyl phosphine (TCEP ...
-
bioRxiv - Microbiology 2019Quote: ... Primers contain a 5’ T7 polymerase promoter used for in vitro transcription from gel purified amplicons using the MEGAshortscript in vitro transcription kit (ThermoFisher Scientific). dsRNA was gel purified prior to use.
-
bioRxiv - Cell Biology 2019Quote: ... The gel was fixed with 40% methanol/10% acetic acid and then stained for 1 h using colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). After in-gel digestion ...
-
bioRxiv - Biochemistry 2019Quote: ... Samples were diluted to equal concentrations and separated using the Novex Native PAGE Bis–Tris Gel system (4–16%; Invitrogen, USA). The gel was vacuum-dried for 2 hours and autoradiographed using a tritium storage phosphor screen.
-
bioRxiv - Cell Biology 2019Quote: ... 3 μl of the final reaction was subjected to electrophoresis on a 1.5% agarose gel using 2xRNA loading buffer (Thermo Fisher Scientific). A 1:1 mix of RNA and loading buffer was heated to 65°C for 5 min and then placed on ice to denature any secondary structure of RNA.
-
bioRxiv - Molecular Biology 2019Quote: ... The gels were washed three times with Milli-Q water for 10 min and stained with PageBlue protein staining solution (ThermoFisher Scientific) for 1 h under gentle agitation ...