Labshake search
Citations for Thermo Fisher :
7951 - 8000 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... resolved by SDS-PAGE and analyzed by immunobloung as described previously (Barkan, 1998) except that precast Tris-Glycine 4-20% polyacrylamide gels (Novex, Invitrogen) were used for gel electrophoresis ...
-
bioRxiv - Biochemistry 2024Quote: RNAs were run for 1 hr at 180V in a 7M Urea 10% acrylamide gel and then transferred to a nylon membrane (Invitrogen) using XCell SureLock Mini-Cell system with the XCell II Blot Module (Invitrogen) ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... Gels were transferred to a polyvinylidene fluoride (PVDF) membrane using the iBlot 2 Dry Blotting System (Thermo Fisher Scientific Inc). Membranes were stained with Ponceau S red solution (Fluka Analytical ...
-
bioRxiv - Microbiology 2024Quote: ... The complexes were then applied to grids for electron microscopy analysis or resolved on 4–12% NuPAGE SDS–PAGE gels (Invitrogen) by staining with Instant Blue (Expedeon) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5 ug of total RNA or 15% of the immunoprecipitated samples was separated on a 10% TBE-Urea gel (Invitrogen), transferred to Zeta-probe (BioRad ...
-
bioRxiv - Biophysics 2023Quote: ... The proteins were transferred from SDS-PAGE gel to the polyvinylidene difluoride membranes by western blotting and were detected using anti-His(C-term)-HRP antibodies (Invitrogen) as described previously (80).
-
bioRxiv - Molecular Biology 2023Quote: ... The eluates from the streptavidin pulldown above were processed by SDS-PAGE using 10% Bis-Tris NuPage Mini-gel (Invitrogen) with the MES buffer system ...
-
bioRxiv - Physiology 2024Quote: ... or lectin-labelled RNA was lyophilized dry and combined with 9 μL of Gel Loading Buffer II (95% formamide, 18 mM EDTA, 0.025% SDS) with a 1x SybrGold (Thermo Fisher), incubated at 55°C for 10 min and finally cooled on ice for 2 min ...
-
bioRxiv - Physiology 2024Quote: ... resuspended in 15 μL of Gel Loading Buffer II (95% formamide, 18 mM EDTA, 0.025% SDS) with 1x SybrGold (Thermo fisher), incubated at 55°C for 10 min and finally placed on ice for 3 min ...
-
bioRxiv - Plant Biology 2024Quote: ... samples were loaded and run on Native PAGE 3%-12% Bis-Tris gels alongside either NativeMark unstained protein standard (Invitrogen) or SERVA Native Marker (SERVA) ...
-
bioRxiv - Plant Biology 2024Quote: Proteins were separated by SDS-PAGE and analyzed by immunoblotting as described previously (Barkan, 1998) except for the use of precast Tris-glycine 4-20% polyacrylamide gels (Novex, Invitrogen). Antibodies to AtpB ...
-
bioRxiv - Cell Biology 2024Quote: ... Five µg of proteins from total plant extracts were solubilized in Laemmli buffer and stacked in the top of a 4-12% NuPAGE gel (Invitrogen). After staining with R-250 Coomassie Blue (Biorad) ...
-
bioRxiv - Cell Biology 2024Quote: ... samples were heated for 10 minutes at 50°C and equal volumes were resolved on 4-12% bis-tris gradient NuPage gels with MES buffer or MOPS running buffer (Invitrogen) and transferred to 0.2 μm PVDF membrane (Millipore ...
-
bioRxiv - Cell Biology 2024Quote: ... A total of 20 ug of protein was loaded per well and electrophoresed through a 10% Bis-Tris NuPage acrylamide gel (Invitrogen), at 120 Volts constant by 2h with MES-SDS running buffer (Invitrogen) ...
-
bioRxiv - Cell Biology 2024Quote: ... Glycerol was added at final concentration of 10% and the sample was loaded onto NativePAGE 3–12% Bis-Tris Protein Gels (Invitrogen). Native PAGE was run using anode buffer (50 mM Bis-Tris HCl pH 7.0 ...
-
bioRxiv - Cell Biology 2024Quote: ... was used to measure protein concentration of each lysate and 50 μg of total protein of each was run on precast Bis-Tris 4–12% gradient gels (Invitrogen) and protein subsequently transferred to polyvinylidene fluoride membrane (Amersham) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Genomic DNA quality was evaluated by gel electrophoresis and quantity was determined using the PicoGreen DNA quantification assays (Life Technologies). Analysis of the DNA was performed using Illumina Infinium Methylation EPIC v1 BeadChips ...
-
bioRxiv - Cell Biology 2024Quote: ... and IFT46 mNeonGreen:VashL were boiled in 1X Laemmli Buffer and run in equivalents on 4-15% continuous gradient SDS-PAGE gels (Invitrogen). Bands were visualized by Coomassie staining and normalized between samples ...
-
bioRxiv - Genomics 2024Quote: ... Concentration of isolated protein was measured using a microBCA kit (Pierce, 23227) and 25µg was loaded on a 4-12% Bis-Tris protein gel (Invitrogen, NP0321BOX). Protein size was analyzed using PageRuler™ Prestained Protein Ladder (Thermo 26616) ...
-
bioRxiv - Plant Biology 2024Quote: ... The multiprotein complexes were separated by a NativePAGE™ 4-16% blue-native-polyacrylamide gel electrophoresis (BN-PAGE) gradient according to the manufacturer’s instructions (Invitrogen) using total amounts of 10 µg chlorophyll/lane.
-
bioRxiv - Genomics 2024Quote: ... Removal of RNA was confirmed through gel electrophoresis followed by gDNA quality and quantity assessment with a nanodrop spectrophotometer and a Qubit fluorometer (Invitrogen). A total of 508 ng and 752 ng of gDNA for D ...
-
bioRxiv - Immunology 2024Quote: Serum was isolated from whole blood collected in 3.5mL BD Vacutainer serum separation tubes containing spray-coated silica and a polymer gel for serum separation (Fisher Scientific). One tube was collected from each participant ...
-
bioRxiv - Cell Biology 2024Quote: ... a total of 30 µg of protein was separated on 4-12% Bis-tris SDS polyacrylamide gels (reducing) and then subjected to dry blot transfer onto nitrocellulose membranes according to the manufacturer’s instructions (Life Technologies). TMPRSS2 was detected by Western blotting using monoclonal anti-TMPRSS2 antibody (clone P5H9-A3 ...
-
bioRxiv - Biophysics 2024Quote: ... samples corresponding to 1 µg Chl were separated on a 4-12% NuPAGE Bis-Tris gel (Invitrogen, Thermo Fisher Scientific) following the manufacturer’s instructions.
-
bioRxiv - Plant Biology 2024Quote: ... The amplified sequence was purified from the gel and was recombined into the donor vector (pDONR221) using BP Gateway cloning according to the manufacturer’s instructions (Fisher Scientific). The donor vector was sequenced and the insert recombined into the destination vector pB7FWG.0 using LR Gateway cloning.
-
bioRxiv - Plant Biology 2024Quote: ... The amplified PCR fragments were purified from the gel and were transferred into donor vector pDONR221 using BP Gateway cloning according to the manufacturer’s instructions (Fisher Scientific). Both donor vectors were sequenced and recombined into four modified pB7WG2 vectors using LR Gateway cloning technology ...
-
bioRxiv - Biochemistry 2024Quote: EV samples were run on 7.5% SDS-PAGE Tris-Glycine gels and stained with Pierce Silver Stain for Mass Spectrometry (Thermo Scientific) according to the manufacturer’s protocol.
-
bioRxiv - Cancer Biology 2024Quote: ... Samples were incubated at 95 °C for 10 min then centrifuged at 14,000 xg for 1 min before loading on Novex 4-20% Tris-Glycine gel (Invitrogen, XP04205). After transfer to PVDF-FL (Merck Life Sciences ...
-
bioRxiv - Plant Biology 2024Quote: ... The amplified fragments were purified from the gel and were transferred into donor vector pDONR221 using BP Gateway cloning according to the manufacturer’s instructions (Fisher Scientific). The donor vector was sequenced to confirm the correct sequence ...
-
bioRxiv - Biophysics 2024Quote: ... The 3-12% native gradient gel in Figure 1A was made using an automatic gradient former and the NativePAGE 3-12% Bis-Tris gel in Supplementary Figure S1A was purchased from Invitrogen. PFO and the vesicle solution containing eggPC:cholesterol 1:1 (molar ratio ...
-
bioRxiv - Biophysics 2024Quote: ... samples corresponding to 1 µg Chl were separated on a 4-12% NuPAGE Bis-Tris gel (Invitrogen, Thermo Fisher Scientific) following the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2024Quote: ... NotI - linearized plasmids were purified (QIAquick Gel Extraction Kit) and the 5’-capped mRNAs were synthesized using SP6 mMessenger mMachine kit (Ambion). For mRNA injection ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Quality of extractions were checked with agarose gel electrophoresis and the quantity of extractions using a Qubit 3 Fluorometric Quantitation Kit (Invitrogen). DNA samples were standardized to (20ng/µl) ...
-
bioRxiv - Molecular Biology 2024Quote: ... An aliquot of this material for synthesized FLEXIs was analyzed on a Novex 10% TBE-Urea polyacrylamide gel (Thermo Fisher) to separate linear and circular RNAs and imaged with SYBR Gold (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The sequence specificity of all primer sets was confirmed by gel purifying a representative subset of qPCR amplicons and either Sanger sequencing amplicons directly or after TOPO-TA cloning (ThermoFisher). A circular form of the 3I_RAN FLEXI synthetic oligonucleotide control was generated by incubating the 5’ phosphorylated-3I_RAN FLEXI oligonucleotide (500 ng ...
-
bioRxiv - Neuroscience 2024Quote: ... An equal amount (10-40 μg per lane) of total proteins from each preparation were loaded and separated on NuPAGE gels (Invitrogen), and transferred to nitrocellulose membranes ...
-
bioRxiv - Molecular Biology 2024Quote: ... The samples were analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and detected using Streptavidin-HRP (Thermo Fisher) or anti-LC serum as previously described.
-
bioRxiv - Molecular Biology 2024Quote: ... The cycle number for library amplification was empirically determined using SYBR Green I Nucleic Acid Gel Stain (Thermo Fisher; S7585) and real-time qPCR tracking (Biorad CFX96) ...
-
bioRxiv - Bioengineering 2024Quote: ... Wells were loaded at 400 ng (standards) or 100 µg total protein/well into a Novex WedgeWell 8% Bis-Tris gel (Invitrogen) and separated in Tris-glycine SDS running buffer (Novex) ...
-
bioRxiv - Microbiology 2024Quote: ... 1.0 µg of total RNA was resuspended in 2x formamide loading buffer (Thermo) and run on a precast 6% PAGE gel (Invitrogen) with the Riboruler RNA ladder (Thermo) ...
-
bioRxiv - Neuroscience 2024Quote: Electrophoresis was performed from 10-20μg of each sample in 4-12% acrylamide- bisacrylamide gradient gels (Invitrogen, Cat. No. NP0322BOX), transferring the samples to nitrocellulose membranes using the iBlot2 dry transfer system (Invitrogen ...
-
bioRxiv - Neuroscience 2024Quote: Total protein from whole cell lysates of mPFC and NAc (6-8 µg) were loaded onto NuPAGE 4-12% Bis-Tris gels (Invitrogen) under reduced and denatured conditions (Pierce 4X LDS sample buffer ...
-
bioRxiv - Bioengineering 2024Quote: ... The line stencil was placed on top of the initial gel and 80 mM calcium chloride (CaCl2 Fisher Scientific, C79) dissolved in either PBS ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... The gel purified fragments were then used as a template for in vitro transcription reactions using Megascript RNAi kit (Invitrogen) or High-Yield T7 RNAi Kit (Jena Biosciences ...
-
bioRxiv - Biochemistry 2024Quote: ... Cytosolic and nuclear extracts and acid-extracted histones were separated on 10% or 4-12% Bis-Tris gels (Life Technologies) and transferred to nitrocellulose membranes (0.2 μm ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The integrity and quantity of RNA was verified through gel electrophoresis and NanoDrop One/OneC spectrophotometer (Thermo Fisher Scientific, US). Total RNA was submitted to Novogene Hong Kong for library construction and sequencing on the Illumina Novaseq6000 (PE150 ...
-
bioRxiv - Evolutionary Biology 2024Quote: Samples containing 1 μg total protein were diluted in 1 x loading dye with 0.1 M dithiothreitol and incubated at 70 °C with shaking at 1000 rpm for 10 mins and then separated by electrophoresis on a 4-12% NuPAGE Bis-Tris gel (Invitrogen) at 120 V for 1 h 35 mins ...
-
bioRxiv - Evolutionary Biology 2024Quote: Samples containing 1 μg total protein were diluted in 1 x loading dye with 0.1 M dithiothreitol and incubated at 70 °C with shaking at 1000 rpm for 10 mins and then separated by electrophoresis on a 4-12% NuPAGE Bis-Tris gel (Invitrogen) at 120 V for 1 h 40 mins ...
-
bioRxiv - Cell Biology 2024Quote: ... Eluates were mixed with protein sample buffer for SDS-PAGE and analyzed with SYPRO Ruby protein gel stain (ThermoFisher S12000), autoradiography or western blotting.
-
bioRxiv - Genomics 2024Quote: ... Protein concentration was measured by the BCA method and 30 µg of protein loaded on 4-12% Bis-tris novex gel (Invitrogen). After electrophoresis ...