Labshake search
Citations for Thermo Fisher :
7901 - 7950 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95°C and 10 µL were then run on a 4–12% Bis-Tris NuPAGE gel (Invitrogen). Bands were visualized by staining with InstantBlue Coomassie Stain (Abcam ...
-
bioRxiv - Molecular Biology 2023Quote: ... was evaluated by running an SDS-PAGE gel (after denaturing for 15 min at 95°C with the addition of denaturing buffer (NP0009, Invitrogen)) with consequent staining using Coomassie blue dye.
-
bioRxiv - Cancer Biology 2023Quote: ... and then cells lysates were prepared using RIPA lysis buffer as described previously.30 Total proteins (8 µg/sample) were loaded onto 4%-20% Novex Tris-Glycine gels (Invitrogen), transferred onto polyvinylidene fluoride membrane ...
-
bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE was performed using NuPAGE 4–12% Bis-Tris gradient gel with a compatible MES SDS buffer system (both from ThermoFisher). Resolved proteins were electro-transferred to a nitrocellulose membrane ...
-
bioRxiv - Cancer Biology 2023Quote: ... 40 µg of cleared protein lysates were resolved by SDS-PAGE on a 4–12% gradient gel (Thermo Fisher Scientific). The proteins were transferred to nitrocellulose membrane (Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2023Quote: Collected cell pellets were lysed in RIPA and separated by electrophoresis on 4-12% NuPAGE Bis-Tris Mini Protein gels (Invitrogen), followed by semi-dry transfer to nitrocellulose membranes using a Power Blotter apparatus (Invitrogen) ...
-
bioRxiv - Microbiology 2023Quote: ... The resulting elution fractions were loaded onto a NuPAGE™ Bis-Tris Gel and run following the manufacturer’s protocol (Invitrogen). Protein bands were allowed to migrate only a short distance of approximately 10 mm ...
-
bioRxiv - Physiology 2023Quote: ... Liver proteins (15 μg) were separated via electrophoresis on a NuPAGE 4-12% Bis-Tris gel (Invitrogen, Carlsbad, CA, USA) and subsequently transferred to a PVDF membrane ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and the appropriate band was purified (ZymoClean Gel DNA Recovery Kit, ZymoResearch) before quantification by fluorescence (Qubit dsDNA HS Kit, Invitrogen).9
-
bioRxiv - Systems Biology 2023Quote: Samples were then heated at 100°C for 10 min and loaded onto a 1.5 mm NuPAGE 4-12% gradient gel (Invitrogen, NP0336BOX). After electrophoresis ...
-
bioRxiv - Biophysics 2023Quote: ... Liquid droplet permeability was studied using 1X SYPRO™ Orange Protein Gel Stain (5,000X Concentrate in DMSO - Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were separated on a 10 % SDS-PAGE gel and transferred to a PVDF membrane using the Power Blotter System (Invitrogen). The following primary antibody was used in this study ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5 ug of total RNA or 15% of the immunoprecipitated samples was separated on a 10% TBE-Urea gel (Invitrogen), transferred to Zeta-probe (BioRad ...
-
bioRxiv - Neuroscience 2023Quote: ... or precipitated conditioned medium) were loaded on precast Nu-PAGE 4 to 12% Bis-Tris protein gels (Thermo Fisher Scientific). After electrophoretic separation ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were separated by SDS-PAGE using 20 μg of protein per well of NuPAGE Novex 4-12% BIS-TRIS gels (Invitrogen) and transferred to 0.2 μm pore nitrocellulose membrane (Amersham ...
-
bioRxiv - Plant Biology 2023Quote: ... samples were loaded and run on Native PAGE 3–12% Bis-Tris gels alongside either NativeMark unstained protein standard (Invitrogen) or SERVA Native Marker (SERVA) ...
-
bioRxiv - Zoology 2023Quote: ... Gels were run in MES SDS running buffer and stained with SimplyBlue™ SafeStain (Thermo Fisher Scientific, Waltham, MA, USA).
-
bioRxiv - Immunology 2023Quote: ... Final mAb samples were also analyzed by SDS-Page gel in order to compare migration of reduced/denatured and not reduced/not denatured samples using the NuPage gel system (Invitrogen) with MES as running buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... 10-15ug total protein was loaded into wells of 3-8% Tris-Acetate gels and separated in tris acetate buffer (Invitrogen) ran at 65v for 30 minutes and then 125v until the dye front reached the bottom of the gel ...
-
bioRxiv - Cell Biology 2023Quote: ... The reactions were quenched with 2 μl of 2.7 M ammonium bicarbonate before loading on Bolt™ 4–12% Bis-Tris Plus gels (Invitrogen) for separation ...
-
bioRxiv - Molecular Biology 2023Quote: ... the reaction mix was cooled to 4°C and analyzed by standard agarose gel electrophoresis with 2% (w/v) Agarose in TAE Buffer with 1:20.000 SybrSafe (Thermo Fisher), followed by imaging with Odyssey Fc at 600nm.
-
bioRxiv - Cancer Biology 2023Quote: ... separation was performed using a NuPAGE Novex 3%–15% Tris-Acetate Mini Gel with 1× NuPAGE Tris-Acetate SDS running buffer (Invitrogen). Following an overnight transfer of proteins onto nitrocellulose membranes ...
-
bioRxiv - Physiology 2023Quote: ... standard glycine-based gel electrophoresis was applied with polyvinylidene difluoride (PVDF) and the following antibodies: SERCA1 (MA3-911; ThermoFisher Scientific), SERCA2 (MA3-919 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10-20µg of protein was loaded per well and run on a 4-12% bis-tris precast sodium-dodecyl sulfate page gel (NP0323BOX, Invitrogen). Semi-dry transfer was performed using the TransBlot Turbo transfer system (1704150 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Specificity and integrity of dsRNA were checked on 1% agarose gel and quantified with a NanoDrop One© spectrophotometer (ThermoFisher). Double-stranded RNA targeting the gene encoding green fluorescent protein (GFP ...
-
bioRxiv - Neuroscience 2023Quote: ... and an equal volume to 0.2 µl of the neat plasma was loaded from DP inputs and FT fractions onto an SDS-PAGE gel (Invitrogen). To enhance protein visualization ...
-
bioRxiv - Developmental Biology 2023Quote: Protein extracts were separated by 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (Invitrogen). Blocking and antibody incubations were performed in 5% bovine serum albumin ...
-
bioRxiv - Molecular Biology 2023Quote: ... A 10-µL sample of fragmented RNA was run on the first-dimension gel in 0.5x Novex TBE running buffer (Invitrogen, LC6675) at 30 W for 9 min ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and an equal amount of lysate was run on SDS–PAGE 4–12% Bis–Tris Protein Gels (Thermo Fisher Scientific) and then transferred to nitrocellulose membrane with a XCell II Blot Module Wet Tank Transfer System (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2023Quote: ... and 10% 2-Mercaptoethanol or 50 mM dithiothreitol (DTT) and run on NuPAGE 4-12% bis-tris gels (Thermo Fisher). Proteins were transferred to nitrocellulose membranes ...
-
bioRxiv - Microbiology 2023Quote: ... Five to 10 μg of total RNA were separated on a 7% (v/v) polyacrylamide gel (7.3 M urea, 1× TBE) and compared to RiboRuler LowRange RNA ladder (ThermoFisher Scientific). RNA from the gel was then blotted on a nylon membrane (Roche ...
-
bioRxiv - Microbiology 2023Quote: ... The mixture was incubated at room temperature for 10 min before addition of G-250 Native Gel Sample Loading Buffer (Invitrogen) to 0.005% final concentration ...
-
bioRxiv - Genomics 2023Quote: ... The average yield of gel-purified DNA from each multiplexed sample of 4 was approximately 50 ng as determined by Qubit (Invitrogen) and Bioanalyzer (Agilent ...
-
bioRxiv - Genomics 2023Quote: ... these reactions were electrophoresed on a non-denaturing 8% PAGE gel alongside O’RangeRuler 10 bp DNA and GeneRuler 50 bp DNA ladders (Thermo Fisher), followed by SYBR gold staining ...
-
bioRxiv - Immunology 2023Quote: ... 10/20µg of protein sample was loaded in the wells of precasted 4-12% polyacrylamide NuPAGE Novex gel (Invitrogen, NP0322BOX) submerged in 1X MOPs running buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 15 μL of each sample was run on two separate 4–12% Bis-TRIS SDS-PAGE gels (ThermoFisher: NP0321BOX). Proteins were then transferred to a nitrocellulose membrane using the Invitrogen iBlot 2 transfer device (ThermoFisher ...
-
bioRxiv - Cancer Biology 2023Quote: ... and samples were boiled at 95°C for 10 minutes and an equal amount of protein lysate (10-20 ug) was electrophoresed through 4–12% SDS-PAGE gels (Invitrogen) and then transferred to PVDF membranes ...
-
bioRxiv - Developmental Biology 2023Quote: ... Eluted proteins were resolved be SDS-page western blotting using 4–12% NuPAGE Bis-Tris gels (Thermo Fisher Scientific, NP0321BOX). Blots were incubated with Rabbit mAb anti-DYKDDDK Tag (HRP Conjugated ...
-
bioRxiv - Cell Biology 2023Quote: Protein size separation of 25 µg of protein was done on a NuPAGE 4-12% Bis-Tris gel (Invitrogen, NP0336BOX) in NuPAGE MES SDS-buffer (Invitrogen ...
-
bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation was performed as described above and proteins were subsequently separated by SDS-PAGE (4-12% NuPAGE tris gel (Invitrogen)) for 7 min at 200 V and stained with Coomassie blue ...
-
bioRxiv - Cell Biology 2023Quote: ... The protein samples were then separated by SDS-PAGE gels and transferred to the PVDF membrane for either immunoblotting with His antibody (Invitrogen) or staining with Ponceau S ...
-
bioRxiv - Cell Biology 2023Quote: ... 20-30ug of total protein per sample was loaded into wells of 4-12% Bis-Tris gels (Genscript or Invitrogen) and separated in MES or MOPS buffer ran at 65v for 30 minutes ...
-
bioRxiv - Genetics 2023Quote: ... The resulting products were confirmed by visualizing on a gel and quantified using either the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) or Qubit fluorometry (Life Technologies) ...
-
bioRxiv - Bioengineering 2023Quote: ... incubated at 85°C for 10 min and loaded on Bolt 4-12% Bis-Tris polyacrylamide gels (Thermo Fisher Scientific). Proteins were electrotransferred to Immobilon-FL polyvinylidene difluoride (PVDF ...
-
bioRxiv - Biochemistry 2023Quote: ... with TcPINK1 KD at 0.5 mg/mL (approximately 10 μM) and SYPRO Orange Protein Gel Stain 5,000X (Thermo Fisher Scientific) at 6X in a 300 mM NaCl ...
-
bioRxiv - Biophysics 2023Quote: A total of 2.5 μg of each nuclear extract was loaded in duplicate on a 4-12% Bis Tris gels (Invitrogen NP0323BOX) with a dye free sample buffer (4X buffer ...
-
Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: ... The equivalent amount of protein from each reaction was also electrophoresed on 4-12% SDS NuPage gels and detected by Simply Blue Safe stain (Invitrogen).
-
bioRxiv - Bioengineering 2023Quote: ... The gels were bisected into horizontal cross-sections using a razor and placed on a number 1.0 glass coverslip (Fisher Scientific) for z-stack scanning ...
-
bioRxiv - Cell Biology 2023Quote: ... The samples were separated on a NativePAGE Novex 3%–12% Bis-Tris protein gel system (Thermo Fisher Scientific, catalog BN1003BOX) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... The lysate was homogenised using a sonicator (10 pulses, five seconds each) and run on NuPAGE 4-12% Bis-Tris protein gels (ThermoFisher). The iBlot 2 Dry Blotting System (ThermoFisher ...