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Citations for Thermo Fisher :
7851 - 7900 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Integrity of extracted genomic DNA was assessed via agarose gel electrophoresis and DNA concentration quantified fluorometrically using the Qubit (Invitrogen) according to manufactures instructions.
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bioRxiv - Microbiology 2023Quote: ... The DNA band at expected size of the circle was extracted by the Qiagen gel purification kit and then cloned into pCR4 Blunt-TOPO vector (Invitrogen) for sequencing.
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bioRxiv - Molecular Biology 2023Quote: ... and 30 μg of sample was loaded into 4-12% SDS-PAGE Novex Bis-Tris gels (Invitrogen Cat No. NP0321). After protein transfer onto 0.45 μM nitrocellulose membrane (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... Sample was heated 5 min at 95 degrees and analysed on a 7M urea 15% acrylamide gel further stained with SYBR Gold (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... Samples were heated 5 min at 95 degrees and analysed on a 7 M urea 15% acrylamide gel further stained with SYBR Gold (ThermoFisher). As a positive control ...
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bioRxiv - Genetics 2023Quote: ... and restriction enzyme digested pUC19-3xP3-Dsred-attB-Gypsy-MCS was purified with a GeneJET Gel Extraction Kit (ThermoFisher Scientific).
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bioRxiv - Genomics 2023Quote: ... Samples were normalized by BCA quantifications and then loaded in 4 12% or 4-20% Novex Tris-glycine gels (Invitrogen) and run at 165V for ∼1 h in Tris-glycine buffer (25 mM Tris and 192 mM glycine ...
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bioRxiv - Developmental Biology 2023Quote: ... 50 µg of protein lysate per lane was then run on a NuPage 4-12% Bis-Tris gel (Thermo Fisher) at 120 V ...
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bioRxiv - Physiology 2023Quote: ... Twenty µg of protein in each sample was resolved by electrophoresis using 4-12% Bis-Tris gels (Thermo Fisher Scientific) and transferred to nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... DNA quality was assessed by agarose gel electrophoresis and quantified using a QuBit 3 Fluorometer (Thermo-Fisher Scientific, Renfrew, Scotland). Amplicon sequencing was then carried out using the universal bacterial and archaeal primer set (515f and 806r ...
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bioRxiv - Biochemistry 2023Quote: ... the buffer was exchanged to HBS buffer (1×HBS pH 7.2, 5% (v/v) glycerol) by gel filtration (Zeba Spin Desalting column, 2 mL, Thermo Fisher). For immunoblotting ...
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bioRxiv - Plant Biology 2022Quote: ... a 1/200 dilution of cells were transferred to fresh media containing 1X SYBR Green I Nucleic Acid Gel Stain (Invitrogen, now Theremo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... and an equal amount of lysate was run on SDS–PAGE 4–12% Bis–Tris Protein Gels (Thermo Fisher Scientific) and then transferred to nitrocellulose membrane with a XCell II Blot Module Wet Tank Transfer System (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were heated at 95°C for 5 minutes before loading on a 4-12% gradient Bis-Tris gel (Invitrogen). MOPS SDS running buffer (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins in the gel fragments were reduced using 5 mM Bond-Breaker TCEP solution (Thermo Scientific, Product No. 77720), alkylated using 100 mM iodacetamide (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein samples were resolved on 4-12% NuPAGE Bis-Tris gel with protein molecular weight standards (PageRuler Prestained Protein Ladder, 10 to 180 kDa, Thermofisher). The gels were transferred onto PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: To detect protein via western blotting 60µg of protein was resolved on SDS-PAGE 4- 12% Bis-Tris Bolt gels (Life Technologies). Protein was transferred onto a nitrocellulose membrane using a Mini Trans-Bolt Cell (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2022Quote: ... were then heated at 95°C for 10 min and resolved on pre-cast 4-12% SDS-PAGE gradient gels (Invitrogen). Transfer of proteins from SDS-PAGE gels to nitrocellulose membranes was performed by dry transfer using the iBlot™ 2 Gel Transfer Device (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... The quality of extracted DNA was visualized using 1% agarose gel electrophoresis and quantified using Quant-iT PicoGreen ds-DNA Assay kits (Thermofisher) and a fluorescent plate reader ...
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bioRxiv - Genomics 2023Quote: ... The quality and quantity of fragmented DNA were verified using agarose gel (1.5%) electrophoresis and Qubit® 4.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... Protein was separated on a 4–12% Bis-Tris gel and transferred using iBlot 2 transfer apparatus (Thermo Fisher Scientific). A 5% milk solution in TBST was used for blocking for 1 hour at room temperature ...
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bioRxiv - Genetics 2023Quote: Proteins from IP eluates solubilized in Laemmli buffer were stacked in the top of a 4-12% NuPAGE gel (Invitrogen), stained with Coomassie blue R-250 (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... One to 20 μg of total protein were separated by NuPAGE™ 4-12% Bis-Tris Gel (Thermo Fisher Scientific) and transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen) and transferred to 0.2 µm PVDF membranes via wet tank or semi-dry method (specified in the supplementary information) ...
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bioRxiv - Cell Biology 2023Quote: ... Total protein (35 µg per sample) was resolved on 10% SDS-polyacrylamide gels and transferred to PVDF membranes (Fisher Scientific).
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bioRxiv - Biophysics 2023Quote: ... denatured by heating for 5 min at 95°C and subjected to electrophoresis using precast NuPAGE Novex 4–12% Bis-Tris Proteins Gels (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... They were resuspended in loading gel buffer (LDS sample buffer reference 84788, supplemented with NuPAGE sample reducing agent reference NP0009, ThermoFisher) and separated on a 4-12% NuPAGE acrylamide gel (reference NP0321BOX ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA and RNA products at various points of the iTP-seq protocol were analyzed on 9% acrylamide (19:1) TBE (90 mM Tris, 90 mM boric acid, and 2 mM EDTA) gels and stained with SyBR Gold (Invitrogen). Inverse toeprints were excised from 12% acrylamide TBE gels using a clean scalpel ...
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bioRxiv - Microbiology 2023Quote: ... Samples were heat-denaturated and reduced as described for purified proteins before loading onto precast NuPAGE 4-12% Bis-Tris gels (#NP0322, Invitrogen). Proteins were migrated for 2-3 h at 120 V in NuPAGE MOPS SDS running buffer (#NP0001 ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 100mM DTT and were heated at 95°C for 5 min prior to resolving (40% of the sample) on a 4-12% Bis-Tris NuPage NOVEX gradient gel (Invitrogen) in 1x Novex NuPAGE MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved on NuPAGE 3-8% (wt/vol) Tris-acetate or 4-12% (wt/vol) Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 100mM DTT and were heated at 95°C for 10 min prior to resolving on a 4-12% Bis-Tris NuPage NOVEX gradient gel (Invitrogen) in 1x Novex NuPAGE MOPS SDS Running Buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The immunoprecipitation elute was running in gradient SDS-PAGE gel and stained with a silver staining kit (Thermo Fisher, #24600).
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed in RIPA buffer and 20 micro-g of total protein was subjected to Western blot analysis using 4-20% gradient gel (Invitrogen). The primary antibodies used in this study are ...
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bioRxiv - Biochemistry 2023Quote: ... The gel was then transferred onto a 0.45 μM nitrocellulose membrane using an iBlot2 (Thermo Fisher Scientific, Waltham, MA, USA) for 7 min at 25 V ...
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bioRxiv - Cell Biology 2023Quote: ... chilled for 5 minutes on ice and loaded onto an agarose gel alongside RiboRuler High Range RNA Ladder (ThermoFisher, #SM1823). The presence of two prominent bands (rRNA ...
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bioRxiv - Biochemistry 2022Quote: ... The quality of the extracted DNA was evaluated on agarose gel and quantified with the Quant-iT dsDNA Assay Kit (Invitrogen) on a Cary Eclipse fluorimeter (Varian/Agilent ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were resolved by SDS-PAGE (approximately 1 × 107 cell equivalents/lane) on NuPAGE bis-Tris 4–12% gradient acrylamide gels (Invitrogen) and transferred to nitrocellulose membrane (Invitrogen) ...
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bioRxiv - Biophysics 2023Quote: ... Synthesis reactions were mixed with SDS-PAGE loading dye and applied to a 1% agarose gel (Ultra-pure agarose, Invitrogen) casted in an Owl B2 system (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... the cell extracts or de-crosslinked samples were subjected to SDS–PAGE separation on NuPAGE 4–12% gels (Invitrogen, NP0321) in MES buffer ...
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bioRxiv - Genetics 2023Quote: ... PCR amplification products were separated and detected by capillary gel electrophoresis on an ABI 3730 Genetic Analyzer (Applied Biosystems Inc.) including a size standard GeneScanTM-500 ROX (Applied Biosystems Inc.) ...
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bioRxiv - Microbiology 2023Quote: ... The first half was treated with 1x SDS loading dye for protein visualisation and incubated at 70°C for 5 minutes and loaded onto a WedgeWell Tris Glycine 4-12% gradient gel (ThermoFisher). Coomassie Brilliant Blue (CBB ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then loaded at equal volume into NuPAGE 4–12% Bis-Tris gels (ThermoFisher Scientific/Invitrogen, Cat. No. NP0336BOX). Gels were then transferred onto nitrocellulose membranes in Towbin buffer (25mM Tris base ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were boiled at 70 °C for 10 minutes and loaded into 4 – 12 % Bis-Tris SDS-PAGE gels (ThermoFisher) at 10 – 20 µg protein / well ...
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bioRxiv - Cell Biology 2023Quote: ... bromophenol blue 0.005%) and extracted proteins were separated by SDS–PAGE in 4–20% SDS–polyacrylamide gel (Thermo Fisher Scientific) and transferred to polyvinylidenedifluoride membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... 10% of the sample was separated on a NuPAGE™ 4-12% gradient Bis-Tris SDS-PAGE gel (Thermo Fisher) in MOPS running buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 100mM DTT and were heated at 95°C for 10min prior to resolving on a 4-12% Bis-Tris NuPage NOVEX gradient gel (Invitrogen) in 1x Novex NuPAGE MOPS SDS Running Buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and a 10-μL sample of each was run on a 1.5% agarose-TAE-ethidium gel alongside 5μL Invitrogen 1 Kb Plus DNA Ladder (Thermo Fisher 10787018) before imaging with a Bio-Rad ChemiDoc MP Imaging System (Ethidium Bromide mode ...
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bioRxiv - Cell Biology 2023Quote: ... Flagellar suspensions obtained through isolation following the described protocol were loaded in ready-to-use 4-12% electrophoresis gels (NuPAGE Bis-Tris Mini, Invitrogen), together with a molecular weight ladder (PAGERuler Plus or Chameleon Duo) ...
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bioRxiv - Bioengineering 2023Quote: ... 15 µg of PBS-soluble protein from the lysates of the cortex was loaded onto precast NuPAGE 4-12% Bis-Tris gels (Invitrogen). For western blotting of the anti-α-synuclein antibodies (cinpanemab and H2.7) ...