Labshake search
Citations for Thermo Fisher :
7801 - 7850 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... then 5 μg total protein in this buffer was separated by SDS-PAGE on a NuPAGE 3-8% Tris-Acetate Midi Gel (Invitrogen). Protein was transferred to a 0.45 μm PVDF Membrane (Cytiva) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Gels were rinsed three times in DI water and then soaked for 1 hr in SimplyBlue SafeStain (Thermo Fisher Scientific) at room temperature with gentle rocking ...
-
bioRxiv - Biophysics 2023Quote: ... The proteins were transferred from SDS-PAGE gel to the polyvinylidene difluoride membranes by western blotting and were detected using anti-His(C-term)-HRP antibodies (Invitrogen) as described previously (80).
-
Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... were incubated at 37°C for 2 h and analyzed by SDS-PAGE on a 3-8% tris-acete NuPAGE gel (Invitrogen) and stained with Pierce Silver Stain Kit (Thermo Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... and both elutions were examined by SDS-PAGE by loading samples in a NuPAGE 4-12% Bis-Tris precast gradient gel (Invitrogen) using MES SDS buffer.
-
bioRxiv - Immunology 2023Quote: ... 10 μL of PageRuler Protein Ladder and 7 μg of each sample of proteins mixed with 5 μL of loading buffer were loaded and separated on a NuPAGE 4 to 12% Bis-Tris Gel (Invitrogen) using MOPS SDS Running Buffer 20X (Life technologies ...
-
bioRxiv - Molecular Biology 2023Quote: Whole cell extracts were resolved using a Novex™ WedgeWell™ 6% Tris-Glycine Mini Protein Gel (Thermo Fisher XP00065BOX). Samples were transferred to a polyvinylidene difluoride (PVDF ...
-
bioRxiv - Molecular Biology 2023Quote: ... Amplified products were confirmed on a 1% Agarose gel stained with 4.6μl SYBR safe DNA stain (Invitrogen, Carlsbad, California, USA), and results were visualized with an UltraViolet gel viewer (Terra Universal ...
-
bioRxiv - Plant Biology 2023Quote: ... The resulting PCR products were gel-purified using the Zymo gel extraction kit (ZymoResearch) and recombined into the Gateway DONR vector pDONR207 using BP Clonase II (Invitrogen) with overnight incubation at 25°C ...
-
bioRxiv - Plant Biology 2023Quote: ... samples were loaded and run on Native PAGE 3%-12% Bis-Tris gels alongside either NativeMark unstained protein standard (Invitrogen) or SERVA Native Marker (SERVA) ...
-
bioRxiv - Plant Biology 2023Quote: ... The protein content of the samples was visualized by staining the gel with SYPRO™ Ruby (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.
-
bioRxiv - Evolutionary Biology 2022Quote: The concentration of the gel excised Round 2 Multiplex-Nested-PCR product was quantified by using the Qubit dsDNA HS Assay kit (Invitrogen) according to manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein concentration was measured by BCA protein assay kit (Pierce) and equal amount from each sample was loaded and separated on 4-12 % Bis-Tris SDS gels (Novex, Invitrogen). The proteins were transferred to PVDF membranes (Novex ...
-
bioRxiv - Microbiology 2023Quote: ... containing a final concentration of 2.5% beta-mercaptoethanol and loaded onto Bolt 4-12% Bis-Tris gels (ThermoFisher Scientific; NW0412) at equivalent concentrations (15-20 µg for cFLIP knockout validation based on available lysates ...
-
bioRxiv - Microbiology 2023Quote: ... PCR amplicons and restriction digestions were purified using the GeneJET Gel Extraction and DNA Cleanup Micro Kit (Thermo Scientific, Germany). All plasmids constructed in this work were analyzed by restriction endonuclease digestion and Sanger DNA sequencing.
-
bioRxiv - Evolutionary Biology 2022Quote: ... 5% Coomassie brilliant blue G-250 was added before loading ~100 μg on a 3-12% Bis-Tris BNE gel (Invitrogen). After electrophoresis (2.5 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... The solution was then replaced with ultrasound gel (HealthCare Plus) and a small cover slip (5 mm diameter; Fisher Scientific) was placed on top ...
-
bioRxiv - Molecular Biology 2022Quote: ... An equal amount of proteins per sample were resolved by SDS- PAGE on NuPAGE Bis-Tris gels (4-12%) (Invitrogen), followed by transfer onto 0.2 μm Nitrocellulose membrane using a Trans-Blot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Immunology 2023Quote: ... added with 50 mM DTT for 10 min at 75 C and loaded onto precast NuPAGETM 4-12% bis-tris protein gels (Invitrogen), or NuPAGETM 12% bis-tris protein gels (these ones were used for LC3 immunoblotting ...
-
bioRxiv - Immunology 2023Quote: ... The purified pooled library was then quantified with a gel-based method using the Low- Range Quantitative Ladder (Life Technologies), dsDNA High-Sensitivity Qubit (Life Technologies) ...
-
bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE gels were electroblotted onto 100% methanol pre-soaked polyvinylidene difluoride membranes (PVDF, 0.45 µm, Thermo Fisher Scientific, 88518) in transfer buffer (25 mM Tris pH 8 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Total RNA (5 μg per lane) was electrophoresed in an agarose-formaldehyde gel and transferred to a 0.45 μM Nylon membrane (Thermo Scientific). After UV crosslinking ...
-
bioRxiv - Microbiology 2023Quote: ... around 3 to 5 μg total RNA from samples were loaded onto a 6% formaldehyde-containing 1% agarose gel in the fume hood with RNA Millenium Marker (Ambion) as RNA ladder ...
-
bioRxiv - Molecular Biology 2023Quote: ... and were heated at 95°C for 10min prior to resolving on a 4-12% Bis-Tris NuPage NOVEX gradient gel (Invitrogen) in 1x Novex NuPAGE MOPS SDS Running Buffer (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10 µl of the eluted proteins were separated by migration on a NuPAGE 4–12% Bis- Tris Protein Gel (Invitrogen) and silver stained ...
-
bioRxiv - Microbiology 2022Quote: ... and total IgG was isolated from post-ART plasma samples using the Melon gel IgG spin purification kit (Thermo Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... The standard curve templates were generated by serial dilution of previously amplified and gel-purified products quantified by Qubit (ThermoFisher).
-
bioRxiv - Molecular Biology 2023Quote: ... 5×106 cell equivalents were loaded into wells on a 3-12% Bis-Tris gradient gel with unstained NativePage protein standards in the first well (ThermoFisher) and run according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: Elutions from anti-GFP nanobody beads in 1.2x LDS/60mM DTT protein sample buffer were fractionated on 4-12% polyacrylamide Bis-Tris NuPAGE gels (Thermofisher Scientific) using MOPS running buffer ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Total DNA quality was checked using gel electrophoresis and yield was quantified using a Qubit DNA Assay Kit (Thermo Fisher). Families were pooled in equimolar concentrations such that each individual was represented equally to generate 4 libraries ...
-
bioRxiv - Neuroscience 2023Quote: ... Then the samples were heated at 95°C for 5 min and loaded onto a pre-cast Polyacrylamide 4-12% gradient gel (Bolt, Invitrogen). The proteins were then transferred onto a nitrocellulose membrane and then blocked with 10% Blotto (Santa Cruz Biotechnology ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were then heated at 100°C for 10 min and loaded onto a 1.5 mm NuPAGE gradient (4-12%) gel (Invitrogen, NP0336BOX). After electrophoresis ...
-
bioRxiv - Developmental Biology 2023Quote: ... Proteins were digested by incubating gel pieces with 25 mM Triethylammonium bicarbonate (TEAB) containing 5 μg/mL Trypsin (ThermoFisher #90058), at 30°C ...
-
bioRxiv - Genomics 2023Quote: Fifty µg of total protein from each sample were separated on 10% Bis-Tris NuPAGE gels (Invitrogen, Carlsbad, CA, USA) with 6X sample buffer containing 300mM Tris-HCl ...
-
bioRxiv - Cell Biology 2023Quote: ... 20 μl of eluted proteins were briefly run on SDS-PAGE (4 min at 160 V, 10% SDS gel [NP0301, Invitrogen]), stained with SimplyBlue SafeStain (LC6065 ...
-
bioRxiv - Neuroscience 2023Quote: ... 30 μg protein/sample was resolved on NuPAGE 4–12% Bis-Tris gels on the NuPAGE electrophoresis system (Life technologies). Proteins were then transferred onto PVDF membranes ...
-
bioRxiv - Cell Biology 2023Quote: ... A portion of each sample was loaded onto a pre-cast Novex 4–20% polyacrylamide TBE gel (Thermo Fisher Scientific), followed by electrophoresis at 150 volts (constant voltage ...
-
bioRxiv - Systems Biology 2023Quote: ... A second purification step was performed in a 1% agarose gell using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific) to remove the original vector used as template in the PCR reaction ...
-
bioRxiv - Molecular Biology 2023Quote: Thermal shift assays were carried out using 4 μM PhPINK1 (residues 115–575) and 5× SYPRO Orange Protein Gel Stain (Invitrogen) in 25 mM Tris (pH 8.5) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and samples were run on reducing 17.5% Phos-tag gels (containing 50 μM Phos-tag Acrylamide AAL-107 (Wako) and 100 μM MnCl2) and NuPAGE 4–12% Bis-Tris gels (Invitrogen). All gels were stained with InstantBlue Coomassie Protein Stain (Abcam).
-
bioRxiv - Biochemistry 2023Quote: Protein constructs were subjected to native electrophoresis on NativePAGE™ Novex® Bis-Tris Gel system following manufacturer’s instructions (ThermoFisher). Briefly ...
-
bioRxiv - Genetics 2023Quote: ... Proteins were resolved by SDS-PAGE in NuPAGE Novex 4-12% Bis-Tris gels and transferred onto nitrocellulose membranes using the iBlot system (Invitrogen). Membranes were then blocked in 50 mM Tris-HCl ...
-
bioRxiv - Plant Biology 2023Quote: ... and then rotated for another 5 min before loading the strip into the well of a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen).
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were heated for 10 min at 100°C and loaded onto a 1.5 mm NuPAGE gradient (4-12%) gel (Invitrogen, NP0336BOX). After electrophoresis ...
-
bioRxiv - Cell Biology 2023Quote: 1% Triton cell lysates were treated with 50mM DTT and 100mM NEM before loading on a 12% Nu-PAGE gel (Invitrogen). Separated proteins were blotted on PVDF membranes (Millipore) ...
-
bioRxiv - Biophysics 2023Quote: ... samples were mixed with 2X Novex Tris-glycine native sample buffer and loaded into NuPAGE 3-8% Tris-glycine gels (Invitrogen) with NativeMark unstained protein standard (Thermo) ...
-
bioRxiv - Biophysics 2023Quote: ... SDS-PAGE analyses were performed at 180V for 75 min using precast 4-20% Tris-HCl polyacrylamide gradient gels (ThermoFisher). In-gel fluorescence was detected using a ChemiDoc MP imaging system (Bio-Rad).
-
bioRxiv - Microbiology 2023Quote: ... The integrity and quantity of RNA samples were confirmed by running RNA samples in 1.5% agarose gel and NanoDrop (Thermo Fisher), respectively.
-
bioRxiv - Molecular Biology 2023Quote: ... LDS sample buffer was added and proteins were resolved on 4-12% NuPAGE Bis-Tris precast gels (Life Technologies, NP0322) alongside PageRuler Plus Prestained Protein Ladder (Thermo Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were loaded onto the gel alongside the Novex™ Sharp Pre–stained Protein Standard ladder (ThermoFisher Scientific, Paisley, UK). Gels were run at 100V until completion of electrophoresis and visualised using silver stain (Thermofisher Scientific ...