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Citations for Thermo Fisher :
7751 - 7800 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Protein samples in lauryl-dodecyl sulfate sample buffer (LDS) were separated by standard SDS-PAGE on 4-12% Bis-Tris gradient gels (Novex Bolt, Invitrogen). Separated proteins were transferred onto PVDF membranes (Milipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Gels were then stained by shaking for 20 minutes in 50 mL TBE and 10,000x SYBR-green (Fisher Scientific, E33075) and washed with water ...
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bioRxiv - Molecular Biology 2024Quote: ... for 10 minutes at 70°C before loading on a Bolt Bis-Tris Plus 4 to 12% gel (ThermoFisher Scientific) for total protein staining or Western blotting ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then incubated at 95°C during 5 minutes and separated by SDS-PAGE using 4-12% Bis-Tris gels (Invitrogen), stained for 1 hour using BioSafe-Coomassie stain (BioRad) ...
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bioRxiv - Cancer Biology 2024Quote: ... 15-35 µg of protein samples were ran on precast 4-15% gradient gels (Bio Rad) and transferred to nitrocellulose membranes (Invitrogen). 4-15% Mini-PROTEAN® TGX™ precast protein gel (Bio-Rad 4561084 ...
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bioRxiv - Microbiology 2024Quote: ... and 20 µL of the supernatant was loaded and run on a NativePAGE 4-16% Bis-Tris gel (Invitrogen BN1002BOX) and transferred to PVDF membrane (pre-wetted in 70% methanol) ...
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bioRxiv - Molecular Biology 2024Quote: ... nuclear lysates of inputs and affinity-purified elution were loaded on a 4-20% Tris-glycine gel (Invitrogen, Cat# XP04205BOX), 10% acetic acid and 50% methanol was used to fix the gel in for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were boiled at 95 °C prior to separation on a 12% NuPAGE Bis-Tris precast gel (Thermo Fisher Scientific) for 15 min at 170 V in 1× MOPS buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... boiled at 95°C for 5 mins and spun at 21 300 rcf for 5 mins prior to loading in NuPAGE Tris-Acetate gels (Invitrogen). Gels were run at 150 V for 1 h and transferred onto 0.2 µm PVDF membranes using the High MW setting on the Trans-Blot Turbo (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... The generated amplicon was purified on an agarose gel and cloned to the pCR2.1-TOPO® plasmid (Thermo Fisher Scientific) according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was gel extracted and sgRNA was in vitro transcribed using the MegaShortScript™ T7 Transcription Kit (Invitrogen AM1354) at 30°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... 5µl samples of the PCR2 products were fractionated on a 2% agarose Tris-Acetate-EDTA (TAE) gel using Sybr Safe dye (Invitrogen) alongside a set amount of 100bp DNA ladder (NEB ...
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bioRxiv - Neuroscience 2024Quote: Samples prepared in loading buffer were run on 12% Bolt Bis-Tris Plus mini gels (ThermoFisher Scientific #NW00120BOX or NW00122BOX) at 165 V for ∼50 min ...
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bioRxiv - Biochemistry 2024Quote: Blue native PAGE analysis was carried out using NativePAGE 3-12% Bis-Tris 1.0 mm Mini Protein Gels (Thermo Fisher). Samples contained 10 μg of protein in NativePAGE Sample Buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... then equal quantities of protein lysate (3-10 µg) were separated by SDS-PAGE using 4–12% gradient gels (Invitrogen) and transferred to PVDF membranes ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Gels were transferred to a polyvinylidene fluoride (PVDF) membrane using the iBlot 2 Dry Blotting System (Thermo Fisher Scientific Inc). Membranes were stained with Ponceau S red solution (Fluka Analytical ...
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bioRxiv - Neuroscience 2024Quote: ... 30 µg of protein from each sample was loaded into Bolt 4-12% Bis-Tris Plus gels (Invitrogen, Waltham, MA). Each gel contained at least one control PN14 sample as a comparison value for calculating fold change from this predetermined baseline ...
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bioRxiv - Microbiology 2024Quote: ... which led to a bright band at expected molecular weight for targeted proteins when the SDS-PAGE gel is visualized under UV (iBright 1500 imaging system, Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... RNA quality was assessed through agarose gel electrophoresis and quantified using a NanoDropTM Lite Spectrophotometer (Thermo Fisher Scientific Inc., USA).
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bioRxiv - Cell Biology 2024Quote: ... Bound proteins and input samples (whole cell lysates) were loaded on Novex NuPAGE 4-12% gradient Bis-Tris gel (Invitrogen) and transferred to Immobilon-P transfer membranes ...
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bioRxiv - Cell Biology 2024Quote: ... 35-50 µg of solubilized membrane fractions were loaded onto pre-cast Blue Native-PAGE gels (3-12% Bis-Tris minigels, Invitrogen) and run using standard protocols138 ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluates from the streptavidin pulldown above were processed by SDS-PAGE using 10% Bis-Tris NuPage Mini-gel (Invitrogen) with the MES buffer system ...
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bioRxiv - Plant Biology 2023Quote: ... Ten microlitres was subjected to SDS-PAGE (NuPAGE™ 4-12% Bis-Tris Mini Gel, Thermo Fisher, Catalogue number: NP0322BOX) at 150 V for 60 min.
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved on precast NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). Samples were transferred to 0.45 µm nitrocellulose membrane (0.9 A ...
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bioRxiv - Genomics 2024Quote: ... cell lysates and formalin-fixed chromatin preparations were run on Tris-Glycine SDS 4-20% WedgeWell gel (Thermo Fisher Scientific) and transferred on nitrocellulose membranes by incubation at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Protein extract was heat-denatured for 10 min at 80°C in NuPAGE LDS Sample Buffer an an equivalent of 2 µg total protein was separated by SDS-PAGE on a NuPAGE 4-12% Tris-Acetate Midi Gel in MOPS buffer (Invitrogen). Protein was transferred to a 0.45 µm PVDF Membrane (Cytiva) ...
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bioRxiv - Neuroscience 2024Quote: ... Twenty grams of each sample were loaded onto a gel (NuPAGE 4 to 12%, Bis-Tris) (Invitrogen, Waltham, MA, USA). NuPAGE LDS sample buffer (1:4 ...
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bioRxiv - Cancer Biology 2024Quote: ... Between 10 to 30 µg of protein were prepared in Laemmli buffer boiled for 5 min and proteins size separated by SDS-PAGE using NuPAGE 10% Bis-Tris 1.5 mm gels (Life Technologies). Proteins were then transferred onto nitrocellulose membranes (Life Technologies ...
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bioRxiv - Biochemistry 2024Quote: Symmetrical nucleosomes produced include AB/AB and AB-apm/AB-apm mutant nucleosomes used for Swc5 quenching and gel shift assays contained yH2B-S115C or xH4-Q27C labeled with Oregon GreenTM 488 Malemide (ThermoFisher) on a 0N0 DNA template43 ...
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bioRxiv - Biochemistry 2024Quote: ... Liquid droplet permeability was studied using 1X SYPRO™ Orange Protein Gel Stain (5,000X Concentrate in DMSO - Thermo Fisher Scientific). Data collection and analysis were performed with the SymPhoTime 64 software (PicoQuant).
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bioRxiv - Evolutionary Biology 2023Quote: ... purity and quantity of the DNA was assessed by running 5μl of the extracted DNA on a 1% agarose gel and by Thermo Scientific NanoDrop spectrophotometer ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products of bisulfite-treated DNA were purified with a Gel Extraction kit (NZY Tech) and cloned into TOPO cloning kit (Thermofisher). Individual clones were sequenced by standard Sanger sequencing and data were analysed by the online tool QUMA 54.
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bioRxiv - Genetics 2024Quote: ... samples were diluted to equal concentrations and heated at 70°C for 10 min before resolution on 12% or 10-20% SDS-PAGE gels (Invitrogen). Resolved proteins were transferred to nitrocellulose membrane in CAPS transfer buffer ...
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bioRxiv - Genetics 2024Quote: ... Samples were boiled at 95°C for 5 min before loading (60 µg) on 4-12% Bis-Tris gel (ThermoFisher) and ran with 1X MES buffer (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... The membranes were placed in an opaque gel box and incubated with blocking buffer (PBST + 10% Fish gelatin (PBS 10X, pH 7.2, Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The gels were stained in 1xTBE with 1:20,000 SYBRTM Gold nucleic acid stain (Thermo Fisher Scientific Corp, Waltham MA) for 30 minutes and imaged on a ChemiDoc MP system (Bio-Rad Laboratories Inc. ...
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bioRxiv - Biochemistry 2023Quote: ... at 70°C for 10 min and run on 10% Bis-Tris precast gels (Thermo Fisher Scientific, Waltham, MA, USA) using NuPAGE MOPS SDS running buffer at 150 V for 1h20 min ...
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bioRxiv - Biochemistry 2023Quote: ... The gels were stained in 1xTBE with 1:20,000 SYBRTM Gold nucleic acid stain (Thermo Fisher Scientific Corp, Waltham MA) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: LC-MS/MS analyses of peptide mixtures coming from in-gel digestions were done using RSLCnano system (Thermo Fisher Scientific) on-line connected to Impact II Qq-Time-Of-Flight mass spectrometer (Bruker ...
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bioRxiv - Genetics 2023Quote: ... Protein concentration was measured using a Rapid Gold BCA kit (Pierce) before being loaded onto Tris- Glycine 4-12% gels (Invitrogen). Gels were transferred using NuPAGE transfer buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Fixed Matrigel containing PDO cultures were gently dissociated by pipetting and embedded in HistoGel Specimen Processing Gel (Thermo Fisher Scientific), followed by standard immunohistochemical procedures as previously described (11).
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bioRxiv - Biochemistry 2024Quote: K562 and HCT116 cell lysates were combined with Laemmli buffer and resolved on Novex WedgeWell 4-20% Tris-Glycine gels (Invitrogen). Gels were transferred to an Immuno-Blot PVDF membrane (Bio-Rad) ...
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bioRxiv - Bioengineering 2024Quote: ... Purity of final preparations was assessed by Tris-Glycine SDS-PAGE 4-12% BT Novex gel with MES running buffer (Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: ... Cre recombinase-mediated deletion of the K700E reporter was assessed on 2% agarose gel in 1xTAE buffer with a 1kB Plus Ladder (Invitrogen). RhAMP SNP primers (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... 15-25 µg of protein was loaded into a 4-12% NuPAGE novex Bis-Tris gradient gel (Thermo Fisher Scientific) and ran at 170 Volts for 1 hour or until ladder was appropriately resolved ...
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bioRxiv - Cancer Biology 2024Quote: ... 10-30ug of whole cell lysate was loaded into each well of 4-12% Bis-Tris NuPage polyacrylamide gels (Invitrogen). Proteins were transferred overnight onto PVDF membranes ...
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bioRxiv - Cell Biology 2024Quote: ... and the lysate was subjected to Western blot on NuPAGE Novex 3–8% Tris-acetate gradient gels (Invitrogen™ # EA0375BOX), transferred to a nitrocellulose membrane and blotted against OPA1 (Anti-Mouse ...
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bioRxiv - Cell Biology 2024Quote: ... Gel pieces of 1cm x 1cm were cut from the expanded gel and attached on 24 mm round Poly-D-Lysine (A3890401, Gibco) coated coverslips to prevent gel from sliding and to avoid drifting while imaging ...
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bioRxiv - Bioengineering 2023Quote: ... Transfer to a nitrocellulose membrane was done using 25 V for 6 min in the iBlot2 Gel Transfer device (IB21001, ThermoFisher). Then ...
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bioRxiv - Biochemistry 2023Quote: ... INPUT and UNBOUND (both 10 % of initial protein extract) and BOUND (IP) fraction were resolved by electrophoresis on acrylamide gels (4-12% Invitrogen) and the transferred onto nitrocellulose membrane that were saturated for 1 hour ...