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Citations for Thermo Fisher :
7701 - 7750 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The DNA was digested with PstI and EcoRI and run in a 1% agarose gel alongside a 1-kb Plus DNA Ladder (Invitrogen) that was 5’-labeled with [γ-32P]-ATP (6000 Ci/mmol ...
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bioRxiv - Genomics 2022Quote: ... DNA quality was examined on a 1% TAE agarose gel and DNA concentration was determined using the dsDNA BR Assay Kit on a Qubit 2 fluorometer (Invitrogen).
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bioRxiv - Immunology 2022Quote: ... the samples were mixed with Coomassie G-250 and resolved in 3-12% NativePAGE Bis-Tris gel (Invitrogen Catalog #BN1003BOX) as per the manufacturer’s recommendations ...
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bioRxiv - Zoology 2022Quote: ... PCR products were visualized using 1.2% agarose gel electrophoresis and purified using the ExoSAP procedure (Exonuclease I - Shrimp Alkaline Phosphatase from ThermoFisher, USA). PCR products were sequenced bi-directionally on an ABI automated capillary sequencer (ABI3130xl ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μl of the Phire PCR reaction was verified on a 1% agarose gel (Ultra-Pure agarose, # 16500-500, Invitrogen), with a 1kb bench top ladder (G754A ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were then purified by agarose gel electrophoresis and subcloned into a pCR2.1 vector with the TA Cloning kit (Life Technologies) according to the supplier’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... last wash and eluate fractions were analyzed by SDS-PAGE on Novex bis-tris 4 to 12% gradient gels (Invitrogen) using MOPS SDS as running buffer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and an equivalent amount (12 μL) of cell lysate from each sample was electrophoresed on a 4-12% NuPAGE gradient gel (Invitrogen) and transferred onto low florescent polyvinylidene difluoride membranes (Bio-Rad) ...
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bioRxiv - Systems Biology 2022Quote: ... Proteins were then denatured by incubating samples at 95°C for 5 min in 4 x Laemmli buffer and dithiothreitol (DTT) before loading onto 4 to 12% NuPAGE gradient precast gels (ThermoFisher). Gels were run for 10 min at 110 V ...
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bioRxiv - Neuroscience 2022Quote: 10 μl of the cleared cell lysate was loaded onto a 4-12% Bis-Tris pre-cast gel (ThermoFisher NW04120BOX) and transferred onto a nitrocellulose membrane using the iBlot 2 dry blotting system (ThermoFisher) ...
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bioRxiv - Neuroscience 2022Quote: ... SDS-PAGE was carried out by resolving the samples by electrophoresis in NuPAGE 4-12% Bis-Tris Protein polyacrylamide gels (#NP0323BOX, Invitrogen). After the electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... an equal amount of protein lysates was subjected to SDS-PAGE 4– 12% gradient gels and electro transferred to a nitrocellulose membrane (iBlot 2, Invitrogen). Membranes were blocked with 5% non-fat milk ...
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bioRxiv - Genomics 2022Quote: ... The quality of genomic DNA and RNA was evaluated using agarose gel electrophoresis and Qubit 3.0 Fluorometer (Life Technologies, USA).
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bioRxiv - Molecular Biology 2023Quote: ... After centrifugation (15,000rpm at 4°C for 10 minutes) samples were boiled in Laemmli buffer and loaded into a 10% MES NuPage Bis-Tris gel (Invitrogen) with the Novex™ SharpPre-stained Protein Standard (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... 4% 2-Mercaptoethanol and Bromophenol Blue) and loaded on NuPAGE 4-12% Bis-Tris Gel (catalog #WG1402BOX; Thermo Fisher Scientific). Proteins were transferred on PVDF membrane (catalog #162-0177 ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μl Coomassie Brilliant Blue G-250 and 2 μl water were added according to the manufacturer’s instructions (NativePageTM Bis-Tris Mini gel electrophoresis protocol, Thermo-Fisher Scientific). Samples were loaded on a NativePAGE™ 3-12% Bis-Tris Protein Gel (Thermo-Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were resolved by SDS-PAGE using Novex NuPAGE pre-cast gradient Bis-Tris acrylamide gels (4–12%) (Invitrogen). After gel electrophoresis ...
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bioRxiv - Physiology 2023Quote: ... Proteins were separated by electrophoresis in NuPAGE™ 4 to 12% Bis-Tris Mini Protein Gels (Thermo Fisher Scientific, USA), and then transferred to Immobilon®-FL PVDF Membrane (Millipore ...
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bioRxiv - Immunology 2022Quote: ... Protein samples were run on 10-12% SDS polyacrylamide gels on a Bio-Rad Protean III system or 4-12% bis-Tris gradient Nu-PAGE gels on a Novex Mini-Cell system (Invitrogen) (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: EMSA experiments using the commercial 10 bp DNA step ladder substrate contained 500 nM total DNA and gels were stained with SYBR Green I stain (Invitrogen) prior to imaging.
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bioRxiv - Neuroscience 2022Quote: 10 μL of normalized lysate with loading buffer was loaded into NuPAGE 4 to 12% Bis-Tris protein gels (Invitrogen) and subjected to 100 V electrophoresis for 65 min ...
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bioRxiv - Neuroscience 2022Quote: ... 50-100 μg of protein in 10-40 μl volume was electrophoresed through 8% or 12% Bolt Bis-Tris Plus gels (InVitrogen) in Bolt MOPS or MES SDS running buffer (ThermoFisher ...
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bioRxiv - Physiology 2022Quote: ... Tissue proteins (15 µg) were separated using electrophoresis on a NuPAGE 4-12% Bis-Tris gel (Invitrogen, Carlsbad, CA, USA) and transferred to a PVDF membrane ...
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bioRxiv - Molecular Biology 2022Quote: Proteins were separated by electrophoresis using a NuPAGE 4-12% Bis-Tris gradient gel run in MES buffer (Life Technologies) and transferred to a Hybond-P PVDF membrane (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: Proteins were separated by electrophoresis using a NuPAGE 4-12% Bis-Tris gradient gel run in MES buffer (Life Technologies) and transferred to a Hybond-P PVDF membrane (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... Worms were immobilized with 20 mM sodium azide and mounted in a 1% low-melting-point agarose gel (UltraPure, Invitrogen). The TagBFP ...
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bioRxiv - Molecular Biology 2022Quote: ... 5μl of each of the three 9-LOX amplicons were mixed with 1μl of 6X gel loading dye (Thermo-Fisher Scientific), loaded on a 1% agarose gel and electrophoresed against a 1kb DNA ladder (RTU-Thermo Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... An equivalent to 100 mg of mitochondrial proteins were loaded and separated on 3–12% (w/v) polyacrylamide NativePAGE Bis/Tris gels (Invitrogen). Following electrophoresis ...
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... The gel was rinsed briefly in DI water before being stained for one hour with 1:5000 SYPRO Red (Invitrogen) diluted in 10% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... then loaded on a 1.0 mm NuPAGE 4-12% Bis-Tris Mini Protein Gel in MOPS buffer with 500 µL of NuPAGE Antioxidant (Invitrogen) in the inner chamber ...
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bioRxiv - Neuroscience 2022Quote: ... were separated on 15% SDS-polyacrylamide gels and blotted onto PVDF membranes (Hybond, Amersham) using the Novex Semi-Dry Blotter (Invitrogen). Primary antibodies were incubated in 5% milk powder in TBST over night at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... then loaded on a NuPAGE 4-12% Bis-Tris Mini Protein Gel in MOPS buffer with 500 µL NuPAGE Antioxidant (Invitrogen) in the inner chamber ...
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bioRxiv - Molecular Biology 2022Quote: 20~60μg lysates from culture cells or mouse tissues were separated on 10% Bis-Tris or 3~8% Tris-Acetate Novex NuPAGE gels (Invitrogen) and transferred to nitrocellulose membrane following standard procedures ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were loaded onto the gel alongside the Novex™ Sharp Pre-stained Protein Standard ladder (ThermoFisher Scientific, Paisley, UK). Gels were run at 100V until completion of electrophoresis and visualised using colloidal coomassie blue (Thermofisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... reduced and alkylated followed by overnight trypsin digestion and peptide extraction following manufacturer’s instructions (In-Gel Tryptic Digestion kit, ThermoFisher Scientific). Extracted peptides were vacuum dried and redissolved in 2% acetonitrile in 0.1% formic acid in water.
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bioRxiv - Cell Biology 2022Quote: ... These prepared samples were loaded onto 3–12% Bis-Tris Native PAGE gels and electrophoresed using a 1× native PAGE running buffer system (Invitrogen). The cathode buffer included 1× cathode buffer additive (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... We then loaded 29 μL of lysed RBC-EVs in NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher Scientific). Separated proteins were transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2022Quote: ... Protein samples were run on 10-12% SDS polyacrylamide gels on a Bio-Rad Protean III system or 4-12% bis-Tris gradient Nu-PAGE gels on a Novex Mini-Cell system (Invitrogen) (Invitrogen). After electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... denatured (70°C for 10min) protein samples (1 µg/µl) were loaded onto a NuPAGE 4-12% Bis-Tris Gel (Invitrogen) along with Chameleon 800 pre-stained protein ladder (LI-COR) ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of CSF were loaded and separated by electrophoresis in a NuPAGE 4–12% Bis-Tris gel (Invitrogen #NP0322), transferred to a nitrocellulose membrane (250 mA ...
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bioRxiv - Systems Biology 2023Quote: ... and 10-15 µg of protein were loaded on 4-12% Bis-Tris Midi NuPage Protein Gels (Thermo Fisher Scientific) and separated at 120V for 2 h in MES running buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... the gels were either stained with 0.5 µg/mL of ethidium bromide solution or 1X SYBR safe (Thermo Fisher Scientific) and followed by de-staining in Milli-Q water for 15 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... 10 μl of the PCR product was loaded onto a 1.5% agarose gel stained with ethidium bromide (Invitrogen, Carlsbad, CA). The electrophoresis was conducted at 100 V for 40 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... resolved by SDS-PAGE and analyzed by immunobloung as described previously (Barkan, 1998) except that precast Tris-Glycine 4-20% polyacrylamide gels (Novex, Invitrogen) were used for gel electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... Glycoproteins were resolved by SDS–PAGE (approx. 1×107 cell equivalents/lane) on NuPAGE bis-Tris 4–12% gradient acrylamide gels (Invitrogen) and transferred either to nitrocellulose or PVDF membranes (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... To remove detergents from the chromatin fractions and to also increase the dynamic range of the analysis the samples were loaded onto a 1.0 mm NuPAGE 4-12% Bis-Tris Gel (Life Technologies). The gel was run for approximately 30 minutes and stained using GelCode Blue Stain Reagent (Thermo Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... The complexes were then applied to grids for electron microscopy analysis or resolved on 4–12% NuPAGE SDS–PAGE gels (Invitrogen) by staining with Instant Blue (Expedeon) ...
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bioRxiv - Biochemistry 2024Quote: RNAs were run for 1 hr at 180V in a 7M Urea 10% acrylamide gel and then transferred to a nylon membrane (Invitrogen) using XCell SureLock Mini-Cell system with the XCell II Blot Module (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... then proteins separated on 4-12% Bis-Tris Bolt gels using electrophoresis before transfer to nitrocellulose membranes (Thermo Fisher Scientific) by iBlot (Invitrogen) ...
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bioRxiv - Physiology 2024Quote: 25-35 μg of protein was loaded onto NuPAGE 4–12% Bis–Tris Midi Gel (Thermo Fisher Scientific, Cat# WG1403BOX) and electrophoresed with the NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific ...