Labshake search
Citations for Thermo Fisher :
7651 - 7700 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... samples were heated (70°C) in Laemmli reducing sample buffer before separation on 4-12% Tris/Bis gradient SDS-PAGE gels (Invitrogen). After electrophoresis ...
-
bioRxiv - Microbiology 2020Quote: ... DNA quality and quantity was assessed by using agarose gel electrophoresis and a Nanodrop ND-2000c (Thermo Fisher Scientific, USA). 16S rRNA gene amplicon sequencing was performed by Novogene Ltd ...
-
bioRxiv - Immunology 2021Quote: ... SDS present on samples were removed by using Extract gel columns and the SDS-Out SDS precipitation kit (Thermo Scientific) (Hirschfield et al. ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were run at 120V for 1.5h and were stained using the Pro-Q Emerald 300 Lipopolysaccharide Gel Stain Kit (Molecular Probes) following manufacturer directions ...
-
bioRxiv - Molecular Biology 2021Quote: Samples were cooled to room temperature and supernatant was ran on Novex NuPAGE 4-12% Bis-Tris Gel (NP0321, Invitrogen). Samples were transferred to nitrocellulose membrane ...
-
bioRxiv - Microbiology 2021Quote: In gel proteolysis by trypsin and analysis by liquid chromatography– tandem mass spectrometry on the Q Exactive Plus (Thermo Fisher) and data analysis with MaxQuant 1.5.2.8 [37] and the UniProt database as the reference were done using a previously described protocol [31] ...
-
bioRxiv - Microbiology 2021Quote: ... concentrated eluates issued from immunopurification of endogenous and RACK1-HA-tagged protein are separated on 10% Tris-glycine SDS-PAGE gels (Invitrogen), and stained with Imperial Protein Stain (Thermo Fisher) ...
-
bioRxiv - Microbiology 2021Quote: ... The PCR products were gel purified and sequenced with an ABI Prism 3700 DNA analyzer (Applied Biosystems, Foster City, CA). The sequences were blasted against the GenBank database.
-
bioRxiv - Immunology 2020Quote: Fractions were pooled based on A280 signals and/or SDS-PAGE on 4-20% Mini-PROTEAN TGX protein gels stained with GelCode Blue Stain Reagent (ThermoFisher). Samples for immunizations were supplemented with 10% glycerol ...
-
bioRxiv - Immunology 2020Quote: ... Cell lysates were centrifuged at 13,000 rpm for 10 min at 4 °C and equal amounts of protein were resolved with a 4 to 20 % Bis-tris Gel/MOPS running buffer system (Invitrogen) and transferred to nitrocellulose membranes ...
-
bioRxiv - Immunology 2020Quote: ... or homogenized with a Bio-Gen PRO200 homogenizer with an attached 5mm X 75mm flat bottom generator probe (Pro-Scientific) and then loaded into 4-12% Bolt Bis-Tris gels (Invitrogen) for gel electrophoresis ...
-
bioRxiv - Immunology 2021Quote: ... Equal amounts of protein were electrophoresed on either 4-12% Bis-Tris or 10% Bis-Tris gels (NuPAGE, Life Technologies) and wet-transferred to nitrocellulose membranes ...
-
bioRxiv - Microbiology 2021Quote: ... PCR products were gel purified and cloned into the pCR4-TOPO vector using the Zero Blunt Topo Cloning Kit (Invitrogen). The clones were sequenced as described above ...
-
bioRxiv - Immunology 2020Quote: ... containing 50 mM dithiothreitol (DTT) for 5 min at 100°C before loading on 8-16% Tris-glycine gels (ThermoFisher). Gels were electrophoresed at RT in running buffer (25 mM Tris ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were sonicated and aliquots containing 5 x 106 cells were separated on a NuPAGE bis-tris 4–12% gradient polyacrylamide gel (Invitrogen) under reducing conditions ...
-
bioRxiv - Immunology 2021Quote: ... The concentrated samples were boiled in a reducing sample buffer and separated in 4-12% polyacrylamide gel (#NW04122BOX, Thermofisher Scientific) electrophoresis (PAGE ...
-
bioRxiv - Neuroscience 2020Quote: ... Supernatants containing the soluble proteins were subjected to SDS-PAGE on NuPAGE™ 4–12% Bis-Tris Protein Gels (Invitrogen) in the Mini Gel Tank and Blot Module Set (Thermo Fisher Scientific) ...
-
bioRxiv - Genomics 2021Quote: ... diluted in 1X TBE) over the surface of the gel and visualized using a Safe Imager 2.0 Blue Light Transilluminator (ThermoFisher Scientific). A high intensity band corresponding to the full length ultramer was cut out of the gel ...
-
bioRxiv - Genomics 2021Quote: ... DNA bands of around 450bp were cut out and purified using GeneJET gel extraction kit according to the manufacturer’s protocol (Thermo Fisher). DNA was eluted in water and then precipitated by adding 0.1 volumes of 3 M NaOAc (pH 5.2 ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were heated at 95 °Cfor 10 min and separated on 4–20% acrylamide Tris-glycine gradient gels (Life Technologies). Proteins were transferred to PVDF membranes (EMD Millipore ...
-
bioRxiv - Cell Biology 2021Quote: ... Ten micrograms of total RNA was mixed with the same volume of Gel Loading Buffer II (AM8546G, Thermo Fisher Scientific) and heated at 65°C for 10 minutes before loading ...
-
bioRxiv - Immunology 2021Quote: ... and purity of final products was confirmed by agarose gels and concentration measured using NanoDrop 2000c (Thermo Scientific ND-2000). Next ...
-
bioRxiv - Molecular Biology 2019Quote: A silver stain of the Tris-Glycine gel was performed using Pierce Silver Stain for Mass Spectrometry (ThermoFisher Scientific 24600). Each sample band in the gel was cut into ∼5 mm squares and was washed overnight in 50% methanol ...
-
bioRxiv - Plant Biology 2020Quote: ... Quality and quantity of bulked DNA was checked by running a DNA sample on an agarose gel (1%) and final DNA concentration determined using the Quibit-4 Fluorometer (dsDNA BR assay, Q32850, Thermofisher).
-
bioRxiv - Microbiology 2021Quote: ... BN-PAGE gels were run for 2 to 2.5 hours at 150 V using the NativePAGETM running buffer (Life Technologies) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2021Quote: ... detunicated and fragments of seminiferous tubules were cultured in agarose gel half-soaked in MEMα culture medium (Gibco A10490-01) supplemented with KnockOut Serum Replacement (KRS ...
-
bioRxiv - Microbiology 2022Quote: ... and F/F proteins in VLPs or in soluble F protein or G preparations were accomplished after their separation in polyacrylamide gels followed by silver staining (Pierce Silver Stain, ThermoFisher) or Western blots of the proteins in parallel with protein standards as previously described (22 ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were then separated by electrophoresis for a short amount of time (5 min) at 200 V on a NuPAGE 4-12% Bis-Tris gel in 1X MES/SDS (Invitrogen) running buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... quenched in 100 mM Tris-HCl pH 8.0 and analyzed on a NuPAGE™ 3–8 % Tris-Acetate gel (Invitrogen). Fractions containing protein bands corresponding to the expected size of a Pol II–APT complex were dialyzed against 10 mM K-HEPES pH 8.0 ...
-
bioRxiv - Cancer Biology 2022Quote: ... The protein lysates were combined with 6× loading buffer containing 2-mercaptoethanol and loaded on NuPAGE 4%-12% Bis-Tris Midi gels (Invitrogen). After separation of protein by SDS-PAGE ...
-
bioRxiv - Cell Biology 2022Quote: ... the samples were loaded (15 μl) in two identically arranged Bolt 4-12% gradient Bis-Tris PAGE gels (Thermo Scientific) – one gel was used for downstream visualization of DDK-tagged proteins while the other gel is for HA-tagged proteins ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were cooled to RT and loaded onto NuPAGE 4-12% Bis-Tris pre-cast gradient gels (Thermo Fisher Scientific) in the buffer-filled blotting module ...
-
bioRxiv - Molecular Biology 2022Quote: Protein samples were resolved on a Bolt 4-12% Bis-Tris protein gel (invitrogen) and transferred to a nitrocellulose membrane with the iBlot 2 dry blotting system (Invitrogen). The following primary antibodies were used for western-blotting at 1/1,000 dilution ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2×104 cells were resuspended in 1.5 ml of growth medium containing 0.4% agarose (4% Agarose Gel, Gibco; top layer) and plated on 1 ml bottom layer containing growth medium and 1.2% agarose in a 12-well plate ...
-
Bipartite viral RNA genome heterodimerization influences genome packaging and virion thermostabilitybioRxiv - Molecular Biology 2022Quote: ... Entire 30 μL of both cellular and supernatant fractions were loaded on to a Bolt 4–12% Bis–Tris Plus Gels (Invitrogen). Details regarding membrane transfer and western blotting were documented previously (Zhou and Routh 2020) ...
-
bioRxiv - Neuroscience 2022Quote: ... of cell or COCS homogenate with or without PNGaseF digestion were mixed with loading dye (ThermoScientific) and loaded on a 4-12% precast NuPage gel (Invitrogen). Transfer to a PVDF membrane (Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: ... The same amount of sample (10 or 30 ng/ml) were loaded onto 4-15% pre-cast Bis-tris gel (Invitrogen) and run at 100V ...
-
bioRxiv - Biochemistry 2022Quote: ... The denaturing gel was run for 2 h at 200 V and RNA bands were visualized with SYBR Safe DNA Gel Stain (Invitrogen) under UV light.
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were size-fractionated on precast 10 cm 4-12% Bis-Tris or Tris-acetate gels (Thermo Fisher Catalog #NP0336BOX), then electroblotted onto nitrocellulose membranes (Thermo Fisher Catalog #88025) ...
-
bioRxiv - Bioengineering 2022Quote: ... To visualize ssDNA patches during time lapse imaging (figure 1) we stained ssDNA with 1-5x SYBR Gold nucleic acid gel stain (S11494, ThermoFisher) in 1x DPBS for 5-10 minutes and washed twice in 1x DPBS before introducing culture medium ...
-
bioRxiv - Biophysics 2022Quote: Protein lysates and immunoprecipitates were subjected to SDS-PAGE on 4–12% NuPAGE Bis-Tris gels (Thermo Fisher Scientific NP0322) using MOPS SDS running buffer (Thermo Fisher Scientific NP0001) ...
-
bioRxiv - Biochemistry 2022Quote: ... 500 μl fractions were collected and 10 μl of the sample of the fractions was taken for analysis on NuPAGE 4%-12% Bis-Tris gel (Invitrogen). Fractions containing the purified protein were concentrated using a 0.5 ml Amicon Ultra centrifugal filter (Merck Millipore ...
-
bioRxiv - Cancer Biology 2022Quote: ... PCR products were analysed using 1-2.5% agarose gels with EtBr and GeneRuler™ 1kB plus DNA ladder (Thermo Scientific). Gels were imaged on a UV gel reader or Bio-Rad ChemiDoc™ Imager.
-
bioRxiv - Cell Biology 2022Quote: ... at 180V on precast either NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen), or NuPAGE™ 3-8% Tris-Acetate gels in NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: Western blots were carried out by running whole cell lysate or co-IP samples on a NuPAGE 4-12% BisTris gel (ThermoFisher) with MOPS running buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... All samples were boiled for 5 min prior to separation by SDS PAGE on precast 3-8% Tris-Acetate NuPAGE gels (Invitrogen). SDS-PAGE fractionated proteins were transferred to a nitrocellulose membrane using a semidry Trans-Blot Turbo Transfer System (Bio-rad) ...
-
bioRxiv - Cell Biology 2022Quote: ... 6* protein loading sample buffer without DTT or SDS was added to samples before loading into a precast native 4-12% tris glycine gel (Invitrogen) without SDS in Tris-Glycine running buffer ...
-
bioRxiv - Genetics 2022Quote: ... Protein concentration was determined with the BCA protein assay kit (Pierce) and 75 μg of protein was resolved by electrophoresis using 12% Bis-Tris Plus NuPAGE gels (Invitrogen) and transferred to PVDF membranes (iBlot 2 system (Invitrogen)) ...
-
bioRxiv - Physiology 2022Quote: ... and equal amounts (10 μg) were loaded into each lane and run on a 4-20% Tris-Glycine gel (Novex, Invitrogen) under reducing conditions ...
-
bioRxiv - Molecular Biology 2022Quote: ... 20 μg total RNA was run on 20% (w/v) acrylamide/8M urea gels with a 32P-labeled Decade marker (Ambion), and then transferred onto Hybond-N membranes (Amersham Pharmacia Biotech) ...