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Citations for Thermo Fisher :
7601 - 7650 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... The gel was run at 100V for almost 3 h then electro-transferred to nitrocellulose membranes using the iBLOT2 system (Invitrogen), and blocked in 5% nonfat milk solution ...
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bioRxiv - Microbiology 2021Quote: ... RNA integrity upon treatment was verified on an 1% agarose gel containing ethidium bromide 10 mg/mL (Invitrogen, ThermoFisher Scientific) and revealed under UV on Gel Doc™ EZ system (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... RNA integrity upon treatment was verified on an 1% agarose gel containing ethidium bromide 10 mg/mL (Invitrogen, ThermoFisher Scientific) and revealed under UV on Gel Doc™ EZ system (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... Thirty μg of protein in each sample was resolved by electrophoresis using 4-12% bis-tris gels (Thermo Fisher Scientific) and transferred to nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... At the end of the incubation the samples were separated under native conditions on BN-PAGE (Native PAGE BisTris Gels System 4–16%, Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... At the end of the incubation the samples were separated under native conditions on BN-PAGE (Native PAGE BisTris Gels System 4–16%, Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... RNA was quality checked on an agarose gel and was quantified using Nanodrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). Following quality validation and quantification using a Life Technologies Qubit Fluorometer (Carlsbad ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were heated at 95°C for 5 min and separated on 4520% acrylamide Tris-Glycine gradient gels (Life Technologies). Proteins were transferred to polyvinylidene difluoride membranes (EMD Millipore ...
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bioRxiv - Molecular Biology 2019Quote: The enriched proteins were resolved by SDS-PAGE on a NuPAGE 4-12% Bis-Tris protein gel (Thermo Fisher Scientific) and stained using the Colloidal Blue Staining Kit (Life Technologies) ...
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bioRxiv - Biochemistry 2019Quote: Carboxylase (with or without FLAG tag) in microsome suspensions was analyzed as above except using a Novex BIS-TRIS 4-12% gel (Invitrogen) and an antibody against the C-terminus of the carboxylase (Pudota 1998 ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amount of protein extract (50-100 µg) was resolved on Bolt™ 4-12% Bis-Tris Plus Gels (Invitrogen) and transferred onto PVDF membrane ...
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miRNA activity contributes to accurate RNA splicing in C. elegans intestine and body muscle tissues.bioRxiv - Molecular Biology 2019Quote: ... The PCR products were analysed using gel electrophoresis analysis and used to perform Gateway BP Clonase reactions (Invitrogen, cat. 11789020) into pDONR P2RP3 as per the manufacturers protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... The gel slurry was then transferred to a Spin-X cellulose acetate column (#60702, Thermo Fisher Scientific; Waltham, MA, USA) and centrifuged for 3 min at 20,000 × gav ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were run on pre-cast 48-well gels and transferred using a semi-dry fast transfer (i-BLOT, Invitrogen). Membranes were blocked in a 50/50 (v/v ...
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bioRxiv - Genetics 2020Quote: ... Protein extracts were run for 30 min at 200 V on denaturing Bolt 4-12% Bis-Tris Plus Gels in 1× Bolt MOPS SDS buffer (Invitrogen), transferred to a nitrocellulose membrane for 1.5 h at 10 V ...
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bioRxiv - Genetics 2020Quote: Proteins eluted from the beads were separated by SDS/PAGE electrophoresis on an 10% polyacrylamide gel and stained with Colloidal blue silver (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA concentration was measured by gel electrophoresis (5μl sample on 1.2% agarose) and by spectrophotometry through Nanodrop 3300 (Thermo Fisher Scientific).
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bioRxiv - Genetics 2021Quote: ... the DNA was purified by separation using electrophoresis on a 1 % (w/v) agarose gel (TopVision Agarose, Thermo Fisher Scientific) in 1x Tris-acetate-EDTA (TAE ...
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bioRxiv - Cell Biology 2019Quote: ... 40 μg were loaded in NativePAGE Novex Bis Tris Gels (3-12 %) and transferred on a PVDF membrane according to the manufacturer’s instructions (ThermoFisher Scientific). Gels were electrotransferred to Hybond-P nitrocellulose membrane (Amersham Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit) and the 5’-capped mRNAs were synthesized using SP6 mMessenger mMachine kit (Ambion). For GFP-centrin ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein (10-30 μg) were separated on 4-12% Bolt bis-tris plus gels (Thermo Fisher Scientific) and transferred to Immobilon FL PVDF membranes (Millipore Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: SDS-PAGE and western blotting were performed using a Novex NuPAGE SDS-PAGE Gel System and XCell II blot module (Invitrogen). Hybond-C Extra membrane (RPN203E ...
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bioRxiv - Neuroscience 2021Quote: ... The same amount of cell or EV lysates were then loaded into 4-12% Bis-Tris plus gels (Invitrogen, NW04125BOX) in reducing or non-reducing conditions ...
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bioRxiv - Cell Biology 2021Quote: ... was crosslinked to the PAAm gels using N-sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate (sulfo-SANPAH, from Life Technologies). 30 μL of 1mg/mL sulfo-SANPAH dissolved in milliQ H2O was added to PAAm gels which were irradiated under a 365nm UV lamp (ABM ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated in 4-12% Nu-Page Bis-Tris (FANCA analysis) or NuPAGE 3-8% Tris-Acetate (FANCD2) polyacrylamide gels (Invitrogen) and transferred to nitrocellulose membranes (Amersham Biotech ...
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bioRxiv - Cell Biology 2020Quote: Western blot analysis was performed by separating 20 μg of protein sample by SDS-PAGE with NuPage Novex 4–12% and 15% (w/v) Bis-Tris gels (Invitrogen). Separated proteins were transferred to Protran nitrocellulose membranes (Schleicher & Schuell ...
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bioRxiv - Biochemistry 2021Quote: The input (total Nav1.5 expression) and biotinylated (cell surface-expressed Nav1.5) samples were separated on NuPAGE 4–12% Bis-Tris gels (Invitrogen, ThermoFisher) and transferred to nitrocellulose membrane (iBlot transfer system ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The quantity and quality of DNA samples were first checked by 1% agarose gel electrophoresis followed by measurement on a Nanodrop spectrophotometer (ThermoFisher) and with a PicoGreen fluorescent dye assay (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... and the samples were boiled for 10 minutes before being separated by electrophoresis using 4 – 12% NuPAGE Bis-Tris gel (Invitrogen). The proteins were then transferred into a PVDF membrane (iBlot 2 Transfer Stacks ...
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bioRxiv - Biochemistry 2021Quote: ... The chromatin pellet was then resuspended in Laemmli loading buffer and was subjected to SDS-PAGE using 4–12% Bis-Tris gradient SDS–PAGE gel (Invitrogen). Control experiments determined that this protocol consistently recovered ∼90% of chromatin added to the extract (data not shown).
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bioRxiv - Biochemistry 2021Quote: ... The agarose gel purified PCR product served as template for in vitro transcription using the MEGAscript® T7 kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... 10-30 µg of samples were loaded onto NuPAGE 4–12% Bis–Tris Midi Gel (Thermo Fisher Scientific, Cat# WG1403BOX) and electrophoresed at 130 V for 2 h with the NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Protein concentration was estimated by the band intensities on a SDS-PAGE gel stained with Coomassie Brilliant Blue and by UV absorbance at 280 nm measured using NanoDrop (ThermoFisher).
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bioRxiv - Cell Biology 2021Quote: Pellets from microtubule cosedimentation were separated using a NuPAGE 12% Bis-Tris gel with MOPS running buffer and stained with Colloidal Blue Staining Kit (Invitrogen). 2 lanes were run for each pellet ...
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bioRxiv - Cell Biology 2021Quote: ... were electrophoresed on precast 4-12% Bis-Tris Plus Bolt Mini gradient gels and electroblotted onto nitrocellulose membranes with the Mini Bolt-iBlot2 system (ThermoFisher). Membranes were blocked in 5% non-fat milk in Tris buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Neuroscience 2021Quote: ... pH 6.8) for 5 min at 95° C and loaded to NuPAGE 4-12% Bis-Tris Protein polyacrylamide gels (Invitrogen, #NP0323BOX). After electrophoresis ...
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bioRxiv - Molecular Biology 2021Quote: ... and the desired 200-600 bp DNA library fragments were selected and isolated using the PureLink DNA gel extraction kit (Invitrogen). Library quality was confirmed using the Agilent 2200 TapeStation nucleic acids system and the Agilent High Sensitivity D1000 DS DNA kit ...
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bioRxiv - Biochemistry 2021Quote: ... Glycoproteins were resolved by SDS–PAGE (approx. 1×107 cell equivalents/lane) on NuPAGE bis-Tris 4–12% gradient acrylamide gels (Invitrogen) and transferred to nitrocellulose membrane (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were run at 200V for an hour through either a 4-12% Bis-Tris or 3-8% Tris Acetate gel (Invitrogen), and then transferred at 300 mA for one hour in 15% methanol 1x Transfer Buffer on a methanol activated PVDF membrane ...
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bioRxiv - Cell Biology 2019Quote: ... 15 ul of the first elution (3% of total) was loaded on NuPAGE 4%–12% Bis-Tris gels (Life Technologies) and analyzed by silver staining.
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bioRxiv - Cell Biology 2020Quote: ... and separated by SDS-PAGE using NuPAGE Bis-tris or Bolt Bis-Tris (12% or 4-12%) precast gels (Invitrogen) and MOPS or MES running buffers ...
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bioRxiv - Cell Biology 2020Quote: ... of cell lysate proteins per lane were resolved on SDS-PAGE (8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis) along with pre-stained protein ladder and transferred onto PVDF membranes (Invitrogen). The transferred membranes were blocked for 1 hour using 5% BSA in TBST (25 mm Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... These prepared samples were loaded on 3–12% Bis-Tris Native PAGE gels and electrophoresed using 1×Native PAGE Running buffer system (Invitrogen). The cathode buffer included 1×Cathode Buffer Additive (Invitrogen) ...
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bioRxiv - Genomics 2020Quote: ... The sample was run on ice at 150V in 1x MOPS Buffer for 75 minutes on a Novex NuPAGE 4-12% Bis-Tris Gel (NP0321, Invitrogen) with 10μL dilute protein ladder (12μL Wash Buffer ...
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bioRxiv - Microbiology 2020Quote: Samples were suspended in diluted with Laemmli sample buffer and typically electrophoresed on a 4–12% gradient SDS-polyacrylamide gel (NuPAGE Novex, Invitrogen). Gels were either stained with Coomassie blue or transferred to a nitrocellulose membrane using an iBlot system (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Aliquots equivalent to 1 μg and 3 µg protein were separated on NuAGE 12% Bis-Tris Gels using MOPS 1X (Invitrogen) running buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... or purified directly from the mix without running it on the gel using a GeneJET PCR purification kit (Thermo Fisher). Purified digested PCR product and digested dephosphorylated vector were ligated together for 2 hours at room temperature using a 1:1 and a 1:3 vector:insert ratio ...
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bioRxiv - Immunology 2020Quote: ... Equal amounts (∼20 μg) of protein extracts were separated by electrophoresis on 4-12% NuPAGE Bis-Tris Mini Gels (Invitrogen) and then transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Amplification products were purified using an Isolate II PCR and gel kit according to manufacturer’s instructions (Invitrogen, Carslbad, California, USA). Sanger sequencing of both strands of each PCR product was carried out commercially ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of total cellular proteins fractionated through 3-8% polyacrylamide Tris-acetate or 4-12% polyacrylamide Bis-Tris gels (Invitrogen) were subsequently transferred to polyvinylidene difluoride (PVDF ...