Labshake search
Citations for Thermo Fisher :
6751 - 6800 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... The blocking buffer was then aspirated and 50 μL of a 1:4000 dilution of rabbit anti-SARS-CoV-2 nucleocapsid (N) antibody (MA536086, Invitrogen) in blocking buffer was added and incubated for 2 h at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... and then incubated for 2 hrs in 40 µL of secondary antibody (1:1,000 anti-rat Alexa Fluor 488, Life Technologies) in 3% BSA in PBS ...
-
bioRxiv - Developmental Biology 2022Quote: ... Epidermal progenitor cells were cotransfected with the luciferase construct and Renilla-luciferin 2-monooxygenase RLuc (in a ratio of 1:10) using Lipofectamine LTX (Thermofisher). The empty pGL3 backbone was used for background control ...
-
bioRxiv - Bioengineering 2022Quote: ... adipose tissue was minced into 1-2 mm pieces and the minced tissue was digested with 200 U/mL Collagenase I (ThermoFisher) at 37 °C in a shaking water-bath for 1 hour ...
-
bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was detected with the CDC assay kit (IDT) and the TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on the QuantStudio3 System (Invitrogen).
-
bioRxiv - Microbiology 2022Quote: Samples were serially diluted (tenfold dilutions starting at a 1:10 initial dilution) in DMEM medium supplemented with 2% FBS containing Antibiotic-Antimycotic (Gibco). The serial dilutions were added to Vero E6 cells seeded in 12-well plates at 3 × 105 cells per well 24 hours previously ...
-
bioRxiv - Biophysics 2022Quote: ... polyacrylamide hydrogel substrates of desired stiffness with 1% of 200 nm fluorescently labeled green beads (2% solid, Thermo Fisher Scientific) were prepared as described previously (82) ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated in primary antibody (rabbit-anti-p65/RELA; 2 ug/mL; ThermoFisher, Waltham, MA, #10745-1-AP) diluted in blocking solution for 1h ...
-
bioRxiv - Neuroscience 2021Quote: ... 50 μL Miltenyi beads per sample were pre-blocked with 48 μL 1× wash buffer and 2 μL yeast tRNA (Invitrogen), rotating for 20 min at room temperature ...
-
bioRxiv - Biochemistry 2021Quote: ... 1-O-(6-BODIPY®558/568-aminohexyl)-2-BODIPY®FL C5-sn-glycero-3-phosphocholine (Thermo Fisher Scientific) as described previously [38] ...
-
bioRxiv - Cancer Biology 2021Quote: ... ionomycin (1 μg/ml) and monensin (2 μg/ml) for 3-4 hours at 37°C in complete IMDM medium (Gibco). Viability staining was performed using the fixable viability dye eFluor780 ...
-
bioRxiv - Cell Biology 2021Quote: NRVMs were derived from 1- or 2-day old Sprague-Dawley rates using the Cellutron Neomyt kit (Thermo Fisher Scientific). Cell purity was routinely >90% cardiomyocytes ...
-
bioRxiv - Cancer Biology 2020Quote: ... organoids were incubated with 2 mM Calcein-AM and 4 mM Ethidium homodimer-1 (LIVE/DEAD assay kit, Molecular Probes) for up to 6h ...
-
bioRxiv - Bioengineering 2021Quote: ... The cornea was then incubated CE facing down on 1 mL of PBS++ containing 2 drops of NucBlue (Life Technologies), 2:100 dilution of mouse anti-ZO-1 antibody (Life Technologies ...
-
bioRxiv - Cancer Biology 2021Quote: ... Internal controls were present in Columns 13 and 14 of each library plate (Pre-miR miRNA Precursor Molecules Negative Control #1 - Ambion AM17110; Pre-miR miRNA Precursor Molecules Negative Control #2 – Ambion AM17111 ...
-
bioRxiv - Immunology 2022Quote: ... Doublets and dead cells were excluded based on forward scatter (FSC) and side scatter (SSC) and 4′,6-diamidino-2-phenylindole staining (DAPI, 1 µg/ml; ThermoFisher). All depicted flow cytometry plots were pre-gated on non-debris (by FSC and SSC) ...
-
bioRxiv - Physiology 2022Quote: ... Counterstaining was performed by incubating with 2 μg/ml DAPI and 1:100 Alexa Fluor™ 488 Phalloidin (ThermoFisher, #A12379) diluted in 0.1% PBSTx for 20 min at RT in the dark ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were plated and transfected simultaneously by collecting cells via trypsinization and diluting to 2x105 cells/mL and mixing with a pre-incubated cocktail of 1:2 volume Lipofectamine 2000 (11668-019, Invitrogen) to 2mM DsiRNA in Opti-MEM (31985-070 ...
-
bioRxiv - Immunology 2022Quote: ... were collected from naive or SNI mice and incubated in a solution of 1 ml of RPMI 1640 medium with 2 mg/ml of collagenase type II (Gibco) for 30 minutes at 37◦C ...
-
bioRxiv - Biochemistry 2022Quote: ... were seeded in a 10- cm culture dish at a concentration of 2 x 105 cells ml-1 (10 ml per dish in DMEM (Nissui) supplemented with 10% fetal bovine serum (Gibco), glutamine ...
-
SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: HEK-293 cells were transfected with the indicated plasmids mixed at a 1:2 (µg DNA : µL transfection reagent) ratio with Lipofectamine2000 (Invitrogen) in OpitMEM (Gibco ...
-
bioRxiv - Developmental Biology 2021Quote: ... DAPI (0.2 mg ml-1) was added to the last wash and coverslips were mounted with ProLong Diamond Antifade Mountant (Invitrogen).
-
bioRxiv - Plant Biology 2020Quote: ... SunTag-VP64 or SunTag-TET1 transgenic plants were selected on 1/2 MS medium + 35 μg/mL Hygromycin B (Invitrogen). The number of leaves at the time of flowering was calculated by counting the total number of rosette and cauline leaves.
-
bioRxiv - Synthetic Biology 2020Quote: ... HEK293T (ATCC CRL-3216), was cultured in complete media (DMEM; 1 g/l glucose, 2 mM L-glutamine, 10 % heat-inactivated FBS (Gibco)) with 5 % CO2 at 37 °C ...
-
bioRxiv - Neuroscience 2020Quote: ... Slices were washed again in PBS and incubated for 2 h at room temperature in secondary antibody in carrier solution (donkey anti-goat 647, 1:1000, ThermoFisher; donkey anti-rabbit 405 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Human globin mRNA was depleted from all samples except from samples #1 and #2 using the GLOBINclear kit (ThermoFisher Scientific). The quality of the RNA was assessed using the Agilent 6000 Pico kit with the Bioanalyzer 2100 (Agilent ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... buffer for 2 hours prior to treatments with either insulin (1 hour) or FGF21 (24 hours) in low glucose media (Invitrogen) supplemented with 0.5% heat-inactivated fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2020Quote: Ex vivo cultures from primary tumor explants were generated by mechanical dissociation and incubation for 1 hour at 37°C with a solution of collagenase IV/dispase (2 mg/mL) (Invitrogen), resuspended in DMEM (Lonza ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 μL rDNase I and 2 μL DNase I Buffer from DNase Treatment and Removal Reagents Kit (ThermoFisher Scientific AM1906) were added to each RNA sample and then incubated at 37°C for 30 min ...
-
bioRxiv - Microbiology 2021Quote: ... ticks of each pool were put into a 2-mL centrifuge tube containing 1 mL of Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Carlsbad, USA) prior to being homogenized in a frozen grinder (Jingxin ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were diluted 1:1000 and were added to the 2 mL liquid MSgg in 24 well plates (Thermo Scientific). The cultures were then grown at 30°C for 72 h in the dark ...
-
bioRxiv - Synthetic Biology 2020Quote: ... All pieces were run on 1 or 2% (w/v) Agarose gel stained with SYBR Safe DNA gel stain (S33102, Invitrogen). Bands at expected sizes were isolated from the gel (740609.50 ...
-
bioRxiv - Neuroscience 2020Quote: ... in a 35-mm culture dish containing 1 ml of medium (DMEM plus 10% fetal bovine serum and 2% B27; Gibco) and were incubated in the cell culture incubator for 1 h to allow recovery from injury ...
-
bioRxiv - Microbiology 2021Quote: ... the formazan crystals were dissolved in 100 µl of a 1:2 mixture of dimethyl sulfoxide (DMSO, Invitrogen, Cat#D12345) and ethanol ...
-
bioRxiv - Immunology 2020Quote: ... gradient centrifugation and resuspended at 1-2 x 106 cells/ml in RPMI-10% FCS (RPMI 1640 w/L-glutamine (Cellgro Gibco) supplemented with 10% FCS (Cellgro Gibco) ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were incubated for 1 h with primary/detection SARS-CoV-2 anti-RBD rabbit polyclonal antibody (Sinobiologicals) and then 1 h with secondary anti-rabbit HRP-conjugate antibody (Invitrogen). Foci were visualised using TrueBlue™ Peroxidase Substrate (Sera Care ...
-
bioRxiv - Neuroscience 2021Quote: ... The sections were then washed and incubated for 2 hours at room temperature in 1:500 Streptavidin protein 488 (21832, Invitrogen). Images were captured using a fluorescence microscope with 1s exposure time and no change of contrast for all pictures to visualize various hypothalamic nuclei ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by a 2 hour incubation period at room temperature with secondary antibody (1:200 Alexa 555 anti-rabbit [Invitrogen] ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary microglial cells were prepared from post-natal day 2-4 pups as previously described and cultured in DMEM containing 1% PSF (ThermoFisher) 10% heat inactivated FBS (ThermoFisher ...
-
bioRxiv - Immunology 2021Quote: ... mice were directly perfused with PBS/ 2 % FCS/ 1 mM EDTA and liver digest medium (ThermoFisher Scientific/ Gibco, Waltham, Massachusetts) through the vena cava ...
-
bioRxiv - Cell Biology 2021Quote: ... HeLa and BJ 1-2 μg of total cell RNA was reverse transcribed into cDNA using SuperScript VILO cDNA Synthesis Kit (11754050, ThermoFisher). A volume corresponding to 25-50 ng of cDNA was used for each RT-qPCR reaction using Roche LightCycler 480 SYBR Green I Master (04707516001 ...
-
bioRxiv - Neuroscience 2021Quote: ... cell pellets were resuspended in 1 mL FACS buffer (DPBS with 2% BSA, Miltenyi Biotec #130-091-376, plus 0.05mM EDTA, ThermoFisher #15575020 ...
-
bioRxiv - Neuroscience 2021Quote: Astrocytes at 3 DIV or at 7-8 DIV after lentivirus infection were loaded with 1 μg/ml Fura-2-AM (#F1221, ThermoFisher) in astrocyte culture medium for 30 min at 37 °C ...
-
bioRxiv - Biophysics 2021Quote: ... were seeded in a 10-cm culture dish at a concentration of 2 × 105 cells mL-1 (10 mL per dish in DMEM (Nissui Pharmaceutical) supplemented with 10% fetal bovine serum (Gibco), glutamine ...
-
bioRxiv - Genomics 2020Quote: ... Cells were electroporated with 2 pulses at 65 mV for 20 ms in a 1 mm cuvette (Fisher Scientific 15437270). After electroporation ...
-
bioRxiv - Microbiology 2021Quote: ... the cells were counterstained in 1 μg/mL DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific GmbH, Bremen, Germany) for 10 min at 46°C ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... RT mixtures were incubated at 52°C for 2 min before addition of 1 μL (200 U) of Superscript III Reverse transcriptase (Invitrogen) and reactions were incubated at 55°C for 2 hr ...
-
bioRxiv - Microbiology 2020Quote: Cells were fixed with 2% PFA at room temperature for 20 min before incubating in 1:10,000 Hoescht 33342 (Life Technologies) in PBS at 4°C overnight ...
-
bioRxiv - Molecular Biology 2021Quote: ... N2a cells were transfected with pSpCas9(BB)-Puro empty vector or vectors expressing each of the three gRNAs targeting the eIF4E3 exons (meIF4E3 gRNA exon 1, exon 2, exon 4) using Lipofectamine 2000 (Invitrogen). pSpCas9(BB)-Puro was a generous gift from Dr ...
-
bioRxiv - Cell Biology 2021Quote: ... and then stained for 30 min with LipidTox Red (1:1000 dilution) or for 15 min with BODIPY 493/503 (2 μg/ml) (Invitrogen) to visualize LDs ...