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Citations for Thermo Fisher :
7001 - 7050 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 25 mM Tris pH 7.4, 2 mM EDTA, 2 mM EGTA, 100 μM PR-619 (Selleckhem, cat. # 7130) and protease inhibitors cocktail (Thermo Scientific, cat. # 784115,) by vortexing ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred to a nitrocellulose membrane (iBlot 2 Transfer Stacks, mini) in an iBlot 2 Gel Transfer Device (Invitrogen; Carlsbad, CA). Immunodetection was carried out using commercially available primary antibodies against PCCA (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... the disulfide bond linking the two Fab fragments of F(ab’)2 goat anti-mouse Ig(G+M) was reduced using 2-mercaptoethylamine HCL (Thermo Fisher Scientific) and biotinylated by using maleimide-PEG2-biotin (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... where (+T) is LNA] was diluted to a final concentration of 2 ng/µl in hybridization buffer [2× SSC (Thermo Fisher Scientific), 0.1 mg/ml yeast tRNA (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos at the desired developmental stage were fixed for one hour using MAD fixative (2 parts methanol [Thermo Fisher Scientific, Waltham, MA], 2 parts acetone [Thermo Fisher Scientific,
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bioRxiv - Biochemistry 2024Quote: ... Peptide separation was performed on an EASY-Spray C18 reversed-phase nano-LC column (Acclaim PepMap RSLC; length - 50 cm; inner diameter - 2 μm; particle size - 2 μm; pore size – 100 Å; Thermo Scientific) at 55 °C and a flow rate of 300 nL/min ...
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bioRxiv - Genomics 2023Quote: ... plugs were melted for 2 minutes at 70°C and solubilized with 2 μL of 0.5 U/μL AGARase enzyme (ThermoFisher Scientific, Waltham, MA) for 45 minutes at 43°C ...
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bioRxiv - Neuroscience 2024Quote: ... Digested ganglia were gently washed once in ‘basal medium’ and twice in ‘complete medium’ (Neurobasal A medium supplemented with 2% B27 [Invitrogen/Thermo Fisher], 2 ng/ml GDNF [Invitrogen/Thermo Fisher] and 10 ng/ml NT4 [PeproTech ...
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bioRxiv - Microbiology 2023Quote: ... the cultures were co-transfected with 2 µg of pCHMP-NS3-Flag and 2 µg of pGag using 4 µL of 293Fectin Reagent (Thermo Fisher Scientific). Cells were pelleted and high-pressure frozen as described in [68] using an HPM100 system (Leica Microsystems) ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections (25 μm thick) from Bregma −2 to +2 (Paxinos and Watson, 2007) were collected onto Superfrost Ultra Plus slides (Thermo Scientific, Germany) air dried at least 2 h at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: ... The colorectal adenocarcinoma Caco-2 cell line and Caco-2 expressing ACE2 were maintained in DMEM+GlutaMAX containing 1mM Sodium Pyruvate (GibcoTM, Thermo Fisher, UK), 10% FBS (GibcoTM ...
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bioRxiv - Microbiology 2023Quote: The purified PCR products were cloned into pCR-XL-2-TOPO™ vectors using the TOPO™ XL-2 Complete PCR Cloning Kit (Invitrogen). After incubation ...
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bioRxiv - Biochemistry 2024Quote: ... Cryo-EM samples were prepared on Quantifoil copper 300-mesh 2/2 holey carbon grids and were plunge-frozen in a Vitrobot Mark IV (Thermo Fisher Scientific) with a chamber temperature of 4°C and a humidity set to 95% ...
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bioRxiv - Immunology 2022Quote: ... and rested for 30 minutes at room temperature in a buffer consisting of 2% FBS in 1X PBS (2% FACS buffer) with 50nM dasatinib (Thermo Fisher Scientific). Cells were washed once with 2% FACS buffer with 50nM dasatinib and incubated with the Gag386-394GW9 and Nef103-111RM9 tetramers for 45 minutes at room temperature ...
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bioRxiv - Genomics 2022Quote: ... Individual DGs were removed from the sting complex using clean forceps and pooled according to treatment group in sterilized 2 mL FastPrep tubes containing six 2 mm zirconia beads and 250 µL Trizol (Invitrogen, Carlsbad, CA). Individual DGs were pooled to obtain sufficient amounts of RNA for downstream analyses ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated for 2-3 hours at room temperature with 2 µg/ml donkey anti-goat Alexa Fluor 488 (Cat# A21206, Thermo Fisher Scientific), 2 µg/ml donkey anti-rabbit Alexa Fluor 568 (Cat# A10037 ...
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bioRxiv - Cell Biology 2023Quote: ... on homemade coverslip-bottomed dishes were washed and loaded with Fura 2 by incubation with Fura 2 AM (4 μM, 45 min, 37°C, ThermoFisher, Cat# F1221), washed and imaged at room temperature in HEPES-buffered saline solution (HBSS) ...
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bioRxiv - Genomics 2023Quote: ... We combined 2 µg of the plasmid mixture with 2 µl of Lipofectamine™ Stem transfection reagent (Thermo Fisher Scientific, cat# STEM00008) and 100 µl of Opti-MEM I (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... sub-confluent HEK293T cells in 24-well plates were transfected with 500 ng of the psiCHECK-2 reporter plasmids mentioned above (G4Q27, G4M27, and native psiCHECK-2) using Lipofectamine 3000 (Invitrogen, ThermoFisher Scientific) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Tryptic peptides (2 µg) were loaded onto a C18 trap (75 µm × 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Fisher Scientific) at a flow rate of 2 µL/min of solvent A (0.1% formic acid and 3% acetonitrile in LC-mass spectrometry grade water) ...
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bioRxiv - Systems Biology 2024Quote: ... Tryptic peptides (2 μ were loaded onto a C18 trap (75 um x 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Scientific) at a flow rate of 2μl/min of solvent A (0.1% formic acid in LC-MS grade water) ...
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Functional and epitope specific monoclonal antibody discovery directly from immune sera using cryoEMbioRxiv - Immunology 2024Quote: ... Cells were resuspended in phosphate buffered saline(PBS) + 2% fetal bovine serum(FBS) 200 ng/mL 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific). Cell sorting was performed on a BD FACSAria Fusion (BD Biosciences).
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bioRxiv - Synthetic Biology 2024Quote: ... subtilis LLO was centrifuged (10,000 x g) for 2 min then resuspended in CellTracker Orange CMRA Dye (CTO, Invitrogen, C34564, 2 µM) in PBS then incubated at 37°C and 250 RPM for 25 min ...
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bioRxiv - Cell Biology 2024Quote: ... The click reaction was performed for 2 hrs at room temperature using 2 μM of the Alexa647 alkyne (Thermo Fisher Scientific, A10278), in a copper-mediated click reaction with CuSO4 ...
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bioRxiv - Cell Biology 2024Quote: ... we changed the medium to a 1:1 mixture of KSR and N2B medium (DMEM F12, Thermo Fisher 11320033, with 1% GlutaMAX, 3% dextrose, N2-Supplement B, StemCell Technologies 07156, 5 μg/mL puromycin, Life Technologies A11138-03, and 2 μg/mL doxycycline). On day three ...
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bioRxiv - Microbiology 2020Quote: ... 2 μL of RNase T1 (0.01 U/μL; Ambion), or 2 μL of Shortcut RNase III (0.02 L/μL ...
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bioRxiv - Microbiology 2020Quote: ... 2% Plant RNA Isolation Aid (Ambion, Woodward, TX, USA), 5 mM ascorbic acid ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 μl Dynabeads Protein G (Thermo Fisher Scientific, 10004D), and 400 μl dilution buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM L-Glutamine (Gibco®, Thermo Fisher Scientific), and a combination of 100 U/ml Penicillin and 100 μg/ml Streptomycin (Gibco® Pen Strep ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM L-Glutamine (Gibco®, Thermo Fisher Scientific), and a combination of 100 U/ml Penicillin and 100 μg/ml Streptomycin (Gibco® Pen Strep ...
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bioRxiv - Developmental Biology 2021Quote: ... 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies #D1306) was used for nuclei staining in conjunction with secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mM L-glutamine (Gibco, Thermo Fisher Scientific). After transfection ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mM L-glutamine (Gibco, Thermo Fisher Scientific). After transfection ...
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bioRxiv - Cell Biology 2020Quote: ... or EVOS FL Auto 2 Cell Imaging System (Invitrogen).
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bioRxiv - Immunology 2021Quote: ... + 2 mM Glutamax + 1x non-essential amino acids (Gibco) + 57µM β-Mercaptoethanol (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mg/ml bovine serum albumin (Thermo Fisher, AM2616), RVC (Ribonucleoside Vanadyl Complex ...
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bioRxiv - Microbiology 2021Quote: ... stained with SYTOX Green (2 μM; Life Technologies, USA), and their DNA content was determined using a FACSAria cytometer (Becton Dickinson) ...
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bioRxiv - Microbiology 2020Quote: ... containing 2% fetal bovine serum (Gibco-Thermo Fisher Scientific) and antibiotic-antimycotic (Gibco-Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... containing 2% fetal bovine serum (Gibco-Thermo Fisher Scientific) and antibiotic-antimycotic (Gibco-Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and 2 hours Proteinase K (Thermo Fisher Scietific #AM2548) treatments ...
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bioRxiv - Biophysics 2020Quote: ... a 2% glucose solution is prepared in DMEM (Gibco). Glucose oxidase (0.5 mg/mL ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM L-glutamine (Thermo Fisher Scientific, Waltham, MA), 50 U/mL penicillin (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Negative control #2 siRNA (Silencer Select, Thermo Fisher Scientific) was used as a negative control.
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bioRxiv - Genomics 2020Quote: ... which contains 2 mg/mL proteinase K (Thermo Fisher), 10 mM Tris (pH = 8.0) ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μl Superscript II First-Strand Buffer (5x, Invitrogen), 0.1 μl MgCl2 (100 mM ...
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bioRxiv - Cell Biology 2022Quote: ... dissolve in 2 μL dry DMSO (Cat# D12345, Invitrogen). The mixture was incubated for 3 days at 37 °C in the dark for conjugation and purified for 3 rounds using Monarch® PCR & DNA Cleanup Kit (5 μg ...
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bioRxiv - Microbiology 2022Quote: ... 2 mM L-glutamine and 10% FBS (Thermo Fisher). HUVECs (Lonza ...
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bioRxiv - Biochemistry 2022Quote: ... and 4U of Turbo DNase (Invitrogen, 2 U/μL) were added to extracts and incubated at 37 °C for 3 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... and 2 mM of L-glutamine (Life Technologies, UK), while for the ECAR measurements the media was supplemented with 1 mM of Sodium Pyruvate and 2 mM of L-glutamine (both from Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... and Bis-acrylamide Solution (2% w/v; 10193523; ThermoFisher) and the level of crosslinking in the gel (between ≍ 40 Pa up to supraphysiological values ...