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Citations for Thermo Fisher :
6551 - 6600 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 2% fetal bovine serum (Gibco, 16-000-044), 200 μM adenosine (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 ml DMEM/F-12 medium (Gibco, 11330107) supplemented with 10% FBS (HyClone ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.1 mM 2-mercaptoethanol (Gibco, Cat# 31350-010), 0.1 mM L-glutamine (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 mM GlutaMAXTM supplement (Life Technologies, 35050-061), 100 µg/mL Primocin (InvivoGen ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated upon shaking at the growth temperature for 5 min with following concentrations: FM 5-95 (2 µg/ml; Thermo Fisher Scientific), DiSC3(5 ...
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bioRxiv - Cell Biology 2021Quote: ... The sgRNA sequences targeting CD33 exon 2 were 5’-TCCATAGCCAGGGCCCCTGT and 5’-GCATGTGACAGGTGAGGCAC.25 U937 cells were washed three times in PBS (Gibco 10010-023) and resuspended in complete Nucleofector Kit C (Lonza Biosciences VCA-1004 ...
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bioRxiv - Pathology 2020Quote: ... Aliquots of 15 μL per sample were loaded at a rate of 5 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) which was equilibrated with 98% Buffer A ...
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bioRxiv - Biophysics 2024Quote: ... for 2 hours at 37°C (with 5% CO2 and humidity) before indicator loading with 5 µM Fluo-4 AM (Invitrogen, Waltham, MA) with 1% PowerLoad Concentrate ...
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bioRxiv - Cancer Biology 2022Quote: ... with or without N-ethyl-N-nitrourea 10μg/ml or 50μg/ml and treated 2h with 1mg/ml Collagenase type IV (Gibco by life technologies ref 17104-019).
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bioRxiv - Plant Biology 2021Quote: ... two SlP4H3 OEX lines (#1, #2) and three SlP4H3 RNAi lines (#1, #6 and #7) by using the TRI reagent (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription of approximately 1 μg of total RNA was performed with Superscript II® Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... and 2-3 μL of the freshly phase-separated sample was placed into a chamber made on a glass slide (Fisher Scientific 3” × 1” × 1 mm). The chamber made by using double-sided tape was then sealed with a square coverslip to avoid evaporation of the sample ...
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bioRxiv - Cancer Biology 2022Quote: ... collected as single cell suspensions using cell strainer and resuspended in sphere formation medium which was composed of 1:1 DMEM and F-12 nutrient mix supplemented with B-27 and N-2 (Thermo Fisher Scientific, Waltham, MA, USA). 1,000 cells were plated in each well of ultra-low attachment 6-well plates (Corning ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells remaining on the bottom of the membrane were fixed in 2% paraformaldehyde and incubated with Hoechst nuclear dye (Invitrogen; 1:10,000 in 1× PBS). Five random 10× magnification fields were imaged ...
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bioRxiv - Molecular Biology 2022Quote: ... Sections were washed thrice with 1× PBS then stained with the secondary antibody goat anti-mouse Alexa Fluor® 555 Plus conjugate (2 μg ml−1, 1:1000-fold dilution; Cat No. A32727, Molecular Probes, ThermoFisher, Waltham, MA, USA) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Alu 1 and MTCO (1 and 2) markers were targeted with qPCR (QuantStudio™ 7 Pro Real-Time PCR System, Applied Biosystems, Massachusetts, United States). Cycling conditions consisted of initial denaturation at 95°C for 2 min ...
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bioRxiv - Plant Biology 2020Quote: ... peptides were reconstituted in 0.1% formic acid and 2% acetonitrile and loaded onto a trap column (C18 PepMap 100 μm i.d. × 2 cm trapping column, Thermo Fisher Scientific) at 5 µL/min for 6 min using a Thermo Scientific UltiMate 3000 RSLCnano system and washed before switching to the analytical column (BEH C18 ...
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bioRxiv - Cell Biology 2020Quote: ... The air-dried DNA was dissolved in 19 µL of sterile water and treated with Swa1 in two cycles (2×2 FastDigest units, Thermo Scientific), each cycle of 1 hour at 37°C (total volume 25 µl ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed twice with PBS/2% FBS prior to resuspension in PBS/2% FBS supplemented with 0.5 µm Sytox blue (ThermoFisher Scientific S11348) to assess cell viability ...
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Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used (8 μg each): F(ab’)2-goat anti-mouse IgG Alexa 647 & F(ab’)2-goat anti-rabbit IgG Alexa 555 (Thermo Fisher). In WGA-Alexa647 labeling experiments ...
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bioRxiv - Genetics 2021Quote: ... using 2 µg of total RNA in the presence of 2 U/µl of RNaseOut Recombinant RNase Inhibitor (Thermo Fisher Scientific). Genomic DNA was eliminated using the RapidOut DNA Removal Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was treated with or without (Mock) RNase R (2 U/μg) in the presence of Ribolock(2 U/μg) (ThermoFisher Scientific). The reaction was incubated at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: Approximately 60 WT and MafAS64F/+ islets from at least 6 female and male mice were analyzed with the ratiometric calcium indicator fura-2-acetoxymethylester (Fura-2 AM) (Life Technologies). Islets were maintained in 5 mM glucose for 30 min prior to measuring 11 mM glucose-induced calcium oscillations and depolarization-activated calcium influx with 30 mM KCl ...
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bioRxiv - Immunology 2021Quote: ... We generatead F(ab’)2 fragments using Pierce™ Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher Scientific #44980). ROS production upon stimulation with or without 10 μM peptide S100A6-1 coated to a Microfluor® 2 white-bottom 96-well plate (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... the concentrations of human IL-2 and IFN-γ were measured using a IL-2 Human Uncoated ELISA Kit (Thermo Fisher) and IFN-γ Human Uncoated ELISA Kit (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: Biotinylated heparin was prepared by mixing 2 mg of heparin and 2 mg of amine– PEG3–Biotin (Thermo Scientific, Waltham, MA) with 10 mg of sodium cyanoborohydride (NaCNBH3 ...
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bioRxiv - Neuroscience 2021Quote: ... the slices were decolored with 100% ethanol for 2 minutes and incubated for 2 minutes in xylene before mounting with Permount mounting medium (Fisher scientific). Brain slices were digitized using a Zeiss Axio Scan.Z1 slide scanner (Carl Zeiss ...
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bioRxiv - Genomics 2021Quote: ... 1 million cells in 6-well plates were transfected with 2 μg of plasmid pT2_FF together with 2 μg of plasmid pCMV(CAT)T7-SB100x and 2 μg of plasmid pcDNA3.1-mCherry using Lipofectamine 2000 (Thermo Fisher, #11668027). After 24 h ...
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bioRxiv - Microbiology 2021Quote: ... Activation of CD4+ T cells was achieved by addition of 25 U/ml of interleukin-2 (IL-2) and CD4+ T-cell activation Dynabeads (Life Technologies). CD4+ T-cells activated for 4-5 days were used for HIV-1 infections ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Microbiology 2020Quote: ... 24 h later the dish was washed with PBS and treated with PR8 virus (MOI =2) in the infection medium [F-12K with 2% BSA fraction V (Thermo Fisher), 1% antibiotic-antimycotic (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... The concentration of IL-2 in 2x diluted supernatants was measured using an IL-2 mouse ELISA kit (Thermo Fisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Peptides were reconstituted in 0.1% formic acid and 2% acetonitrile and loaded onto a trap column (C18 PepMap 100 μm i.d. × 2 cm trapping column, Thermo Fisher Scientific) at 5 µL/min for 6 min using a Thermo Scientific UltiMate 3000 RSLCnano system and washed for 6 min before switching the precolumn in line with the analytical column (BEH C18 ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Biophysics 2022Quote: ... the glass coverslips were mounted onto a Warner chamber and the cells were loaded with 2 µM Fura-2/AM (Molecular Probes) in Ca2+ Imaging Buffer (Ca2+ IB;10 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... The samples were loaded in loading buffer (2% MeCN, 0.05% trifluoroacetic acid) and bound to an Acclaim Pepmap 100 µm × 2 cm trap (Thermo Fisher Scientific), and washed for 10 min to waste ...
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bioRxiv - Neuroscience 2020Quote: Cultured DRG neurons (18-48 hours) were loaded with 2.5 μM Fura-2-acetoxymethyl ester (Fura-2; Invitrogen, ThermoFisher Scientific, #F1221) in 0.01% pluronic F-127 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: Cultured DRG neurons (18-48 hours) were loaded with 2.5 μM Fura-2-acetoxymethyl ester (Fura-2; Invitrogen, ThermoFisher Scientific, #F1221) in 0.01% pluronic F-127 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... HeLa cells seeded on cover glasses were transfected with plasmids encoding full length SARS-CoV-2 spike protein for 2 days using Lipofectamine 2000 (Thermo Fisher). Cells were fixed with 2% formaldehyde in PBS for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2021Quote: ... Japan), 50 μg/mL streptomycin (Meiji Seika Pharma, Japan) and 0.1 mM 2-mercaptoethanol (2-ME; 21985-023, Thermo Fisher Scientific). For the maintenance of PKA-ESCs ...
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bioRxiv - Biochemistry 2020Quote: ... Primary antibodies were removed by 3 washes with 2% BSA 1x PBS and incubated with 2 μg/ml anti-rabbit IgG Alexa Fluor 594 (Thermo Fisher) for 2 h in the dark at 4°C ...
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bioRxiv - Genetics 2021Quote: ... and 1.5 µg of each of the sgRNAs plasmids (PX330-Nanog-sgRNA1/2 and PX330-Esrrb-sgRNA1/2) using 16.5 µl of lipofectamine 3000 and 22 µl of P3000 (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... cell pellet of 1 x106 – 2 x106 PBMCs was resuspended in 2 ml erythroblast cell expansion media containing basal blood media Stempo34 SFM (Thermo Fisher) supplemented with 1x Stempo34 nutrient supplement (Thermo Fisher) ...
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bioRxiv - Immunology 2020Quote: T or B cells (2-4 × 107) obtained from 2 spleens of actin-GFP mice by negative MACS kits (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The agarase-treated samples were then carefully poured into combing reservoirs containing 1.2 ml of MES solution supplemented with 2 mM Zn(O2CCH3)2 and either S1 nuclease (40 U/ml) or S1 nuclease dilution buffer (Thermo Fisher) and were incubated for 30 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... Filters were first cut into 2 mm by 2 mm pieces using scissors sterilized and treated with RNase AWAY surface decontaminant (Thermo Scientific). Filter pieces were transferred into MN Bead Tubes Type A (Macherey-Nagel ...
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bioRxiv - Biochemistry 2022Quote: ... were added to Au-flat 2/2 (2 µm hole, 2 µm space) cryo-electron microscopy grid (Protochips, Morrisville, NC, USA) using a Vitrobot Mk IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Microbiology 2022Quote: ... and the resulting peptides were collected on a trap column (2 mm × 2 cm) with POROS 20 R2 material (Thermo Scientific) at 0.5°C ...
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bioRxiv - Neuroscience 2023Quote: ... The following day cells were rinsed with PBS and incubated for 2 hours with the appropriate Alexa Fluor-conjugated secondary antibodies (2 μg/mL, Thermo Fisher) and DAPI (1 μg/mL ...