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Citations for Thermo Fisher :
6601 - 6650 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... NPCs were cultured in 2% B-27-supplemented Neurobasal CTS Media (plus 2 uM L-glutamine and 0.2% vol/vol penicillin–streptomycin; Thermo Fisher Scientific) plus 20 ng/ml BDNF ...
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bioRxiv - Immunology 2024Quote: ... Efferocytosis was quantified by incubating BMDMs with Jurkat T cells (MOI 2, 45 min) which had been labelled with calcein-acetoxymethyl (2 h, 37 °C, ThermoFisher Scientific) and exposed to UV light (305 nm ...
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bioRxiv - Physiology 2024Quote: ... only 4 wells of cells were incubated in DMEM with 4 μM fura-2/AM (fura-2/AM, Molecular Probes, USA) at 37° C in the dark for 45 min ...
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bioRxiv - Neuroscience 2024Quote: ... Digested ganglia were gently washed once in ‘basal medium’ and twice in ‘complete medium’ (Neurobasal A medium supplemented with 2% B27 [Invitrogen/Thermo Fisher], 2 ng/ml GDNF [Invitrogen/Thermo Fisher] and 10 ng/ml NT4 [PeproTech ...
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bioRxiv - Physiology 2023Quote: ... The final plasmids for the sense and antisense expression of daf-2 or aak-2 under the str-3 promoter were generated using Gateway Cloning Technology (Life Technologies) and injected into FLP-7 secretion line (SSR1164 ...
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bioRxiv - Immunology 2024Quote: ... Digestion was stopped by adding acetonitrile to 2% and trifluoroacetic acid to 0.3% and the beads were collected with DynaMag-2 Magnet (Thermo Fisher Scientific). Digested peptides were purified by loading the supernatant into C18 StageTips (58) ...
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bioRxiv - Biochemistry 2024Quote: ... were applied onto freshly glow discharged R 2/2 UltrAuFoil grids prior to plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) with a blot force of 0 and 5 sec blot time at 100% humidity and 22°C ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of 1.1 mg/mL or 0.5 mg/mL of complex were loaded onto freshly glow discharged R 2/2 UltrAuFoil grids84 prior to plunge freezing using a Vitrobot Mark IV (ThermoFisher Scientific) with a blot force of 0 and 6 sec blot time at 100% humidity and 22°C ...
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bioRxiv - Bioengineering 2024Quote: ... and RSV B/ 18537 DS-Cav1 (51) at 2 µg/mL were coated onto 96 well ELISA plates (Immulon 2 HB, Thermo Fisher) at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... One-tenth volume of cDNA (2 µl) was added to qPCR reactions containing target specific primers (Table 2) and SYBR green master mix (Applied Biosystems) as per manufacturer’s specification ...
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bioRxiv - Microbiology 2022Quote: ... U-2 OS cells were transduced with lentiviral vector encoding shBmal1 and cells selected using 2 μg/ml of puromycin (Life Technologies).
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bioRxiv - Genetics 2023Quote: ... RNA pellets were resuspended with 16 µL of nuclease-free H2O and treated by DNase (2 µL of DNase buffer and 2 µL of TURBO DNase; Thermo Fisher) at 37 °C for 1 h ...
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bioRxiv - Immunology 2023Quote: ... cells were stained at 37°C for 30 min with 2 µM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCF-DA; Thermo Fisher Scientific) or 1 µM MitoSOX (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... sub-confluent HEK293T cells in 24-well plates were transfected with 500 ng of the psiCHECK-2 reporter plasmids mentioned above (G4Q27, G4M27, and native psiCHECK-2) using Lipofectamine 3000 (Invitrogen, ThermoFisher Scientific) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 variants were identified by the targeted sequencing approach with the Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The BALF cell pellet was treated with red cell lysis buffer and re-suspended in Hank’s Balanced Salt Solution (HBSS) supplemented with 2% fetal bovine serum (FBS) (2% FBS/HBSS; Gibco, CA, USA) for cell counting and flow cytometry analysis.
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bioRxiv - Biochemistry 2023Quote: ... The peptic peptides thus generated were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Physiology 2023Quote: ... Sense and antisense sequences targeting daf-2 or aak-2 were amplified from N2 lysates and subcloned into donor vectors using Gateway Cloning Technology (Life Technologies). The final plasmids for the sense and antisense expression of daf-2 or aak-2 under the str-3 promoter were generated using Gateway Cloning Technology (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... The culture dish was coated with 0.2% gelatin and the cells were cultured in endothelial growth medium (EGM)-2 complete media (#CC-3162, Lonza Clonetics, Fisher scientific) with EGM-2 SingleQuots supplement kit (CC-4176 ...
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bioRxiv - Microbiology 2023Quote: ... after which metal beads with DNA absorbed on their silica surface were recovered using the DynaMag™-2 Magnet tube holder (12321D-DynaMag-2 – Invitrogen) and washed twice with AMMLAV/E buffer (10 mM Tris pH 8 ...
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bioRxiv - Microbiology 2023Quote: 3 μL of 3 mg/mL purified Umb1 particle samples were loaded onto freshly glow discharged R 2/2 UltrAuFoil grids prior to plunge freezing using a Vitrobot Mark IV (ThermoFisher Scientific) with a blot force of 0 and 6 sec blot time at 100% humidity and 22°C ...
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bioRxiv - Microbiology 2023Quote: ... The resulting peptic peptides were collected on a trap column cartridge (2 mm x 2 cm) that was filled with POROS 20 R2 reversed-phase resin (Thermo Scientific) and kept at 0.5 °C for 3 min ...
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bioRxiv - Cell Biology 2024Quote: ... 11.2 and 20.2 µg/mL) concentrations as well as the following additional small molecule inhibitors were collected: puromycin (2 µg/mL; ThermoFisher, A1113803), rapamycin (9.1 nM ...
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bioRxiv - Cancer Biology 2024Quote: ... an IonPac AS24 analytical column and IonPac AG24 guard column (2 x 250 mm and 2 x 50 mm, Thermo Scientific) were connected to a Dionex Ultimate 3000 HPLC system (Thermo Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 μg/mL Penicilin-Streptomicin) supplemented with 2 mM L-Glutamine (ref.07100, Stemcell) and 10 mM 2-Mercaptoethanol (ref.11528926, Thermo Fisher). The medium was partially changed every other day extremely slowly to prevent disturbing the EB formation ...
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bioRxiv - Immunology 2024Quote: ... 2.7 μl of the mixture was pipetted to Quantifoil R 2/2 gold grids with vitrobot Mark IV (Thermo Fisher Scientific) using parameters set to 4 °C with 95% humidity and vitrified with no wait time ...
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bioRxiv - Microbiology 2024Quote: ... were applied onto freshly glow discharged R 2/2 UltrAuFoil grids72 prior to plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) with a blot force of 0 and 5.5 sec blot time at 100% humidity and 22°C ...
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bioRxiv - Genomics 2024Quote: ... Slides were blocked with 2% BSA in PBS and 4,6-diamidino-2-phenylindole (DAPI; D1306, Thermo Fisher Scientific, Waltham, MA, USA) for 5 minutes and permeabilized with 0.1% Saponion (Thermo ...
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bioRxiv - Biochemistry 2024Quote: ... were applied onto freshly glow discharged R 2/2 UltrAuFoil grids93 prior to plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) with a blot force of 0 and 5.5 sec blot time at 100% humidity and 22°C ...
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bioRxiv - Immunology 2024Quote: ... and subsequently exocrine-free islets were selected and dispersed with 0.02 % trypsin for 3 min at 37 °C and washed with FACS buffer (2% FBS, 2 mM EDTA (Thermo Fisher Scientific) in PBS (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... The dishes were then filled with 2 mL of serum-free DMEM-F12 medium supplemented with 2 mM thymidine and 0.02 mg rutin (Acros Organics, #132390050) to avoid GFP photobleaching.
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bioRxiv - Cell Biology 2024Quote: ... Japan), 50 µg/mL streptomycin (Meiji Seika Pharma, Japan) and 0.1 mM 2-mercaptoethanol (2-ME; 21985-023, Thermo Fisher Scientific). The culture dish was pre-coated with 0.1% gelatin at 23 °C for 5min ...
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bioRxiv - Immunology 2020Quote: ... L-lysine-4,4,5,5-D4 (Gibco, Cat# 88438), L-arginine-13C6 (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... L-Lysine-2HCL,4,4,5,5-D4 (ThermoFisher Scientific) and 13C-labelled L-Arginine HCl (201203902 ...
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bioRxiv - Microbiology 2021Quote: ... which was boiled in the SDS loading buffer with 5% β-mercaptoethanol (Cat. #60-24-2, Acros Organics). The denatured protein samples were separated by Novex™ WedgeWell™ 4-20% SDS-PAGE Tris-Glycine gel and transferred to PVDF membrane (iBlot™ 2 Transfer Stacks ...
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bioRxiv - Genomics 2020Quote: ... Here, 40 μL pre-mix (5 μL 10x NEBuffer 2.1, 2 μL 100 mM ATP (Thermo Fisher, R0441), 3 μL 100 mM DTT ...
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bioRxiv - Developmental Biology 2021Quote: ... These vectors were co-transfected into GATA3 reporter cells (G3KI#5-2) using the Lipofectamine Stem reagent (Invitrogen). Two days after transfection ...
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bioRxiv - Immunology 2021Quote: ... we measured the proliferation capacity of NK92 cells by an EdU (5- ethynyl-2’-deoxyuridine; Invitrogen, California, USA) proliferation assay ...
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bioRxiv - Biochemistry 2020Quote: ... and an Acclaim PepMap 100 trap column (100 μm × 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific) were used to separate tryptic peptides by increasing concentrations of 80% acetonitrile (can ...
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bioRxiv - Microbiology 2021Quote: ... and an Acclaim PepMap 100 trap column (100 µm × 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
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bioRxiv - Microbiology 2021Quote: ... the monolayers were overlaid with 2 ml of Minimal Essential Medium (Mediatech) supplemented with 5% FBS (Life Technologies), nonessential amino acids (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: NO production was detected by DAF-FM diacetate (4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate) (D23844, ThermoFisher). Fly larvae were dissected at 24 or 48 h AI in PBS to expose the sensory neurons ...
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bioRxiv - Bioengineering 2022Quote: ... and blocked for 2 hours with a solution containing 0.5% TritonX-100 and 5% donkey serum (Thermo Fisher). Primary antibodies used were rat CD68 (FA-11 ...
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bioRxiv - Microbiology 2020Quote: Cells grown until A600 of ∼0.1 were labelled with 10 μM EdU (5-Ethynyl-2’-deoxyuridine, Thermofisher, C10337) for 15 min after which cells were washed ...
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bioRxiv - Neuroscience 2021Quote: ... Pipettes had resistances of 5–7 MΩ when filled with this solution supplemented with Fura-2 (Molecular Probes). Recordings were made using a Multiclamp 700B amplifier (Molecular Devices ...
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bioRxiv - Immunology 2020Quote: ... the proliferation capacity of NK92 cells was measured by an EdU (5-ethynyl-2 ‘-deoxyuridine, Invitrogen, CA, USA) proliferation assay ...
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bioRxiv - Biophysics 2021Quote: ... Cells were incubated with 10 μM of the synthetic nucleoside 5-Ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher Scientific) for 25 min at 37°C and 5% CO2.
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bioRxiv - Cancer Biology 2020Quote: ... Samples were then treated with ExoSAP-IT enzyme (2 μl of enzyme for 5 μl of sample, ThermoFisher) at 37 °C for 15 min followed by a 15 min inactivation at 80 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU)) staining were performed using the Click-iT Plus EdU Assay kit (C10339; Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Probes consisting of oligodeoxyribonucleotides (see Supplementary Table 2) were 5′-end labeled using T4 polynucleotide kinase (ThermoFisher Scientific) with 25 μCi of [γ-32P]dATP ...