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Citations for Thermo Fisher :
6801 - 6850 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 1-2 × 105 cells were washed with facs wash (FW, PBS supplemented with 1% hiFCS and 0.05 mM EDTA (ThermoFisher Scientific)) and stained in FW with antibodies either 30 min on ice or 15 min at room temperature in the dark ...
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bioRxiv - Genomics 2021Quote: ... tumors and adjacent non-cancer tissues were cut into approximately 1-2 mm3 pieces in the RPMI-1640 medium (Gibco), and enzymatically digested with gentleMACS (Miltenyi ...
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bioRxiv - Genetics 2020Quote: ... maintenance in mTesR1 medium for 1-2 days was required before puromycine selection (0.8μg/ml puromycine [ThermoFisher] for 24 hours). 24-hour puromycine selection was made for every passage.
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bioRxiv - Neuroscience 2020Quote: ... We used a Nanoject II to inject 0.2 – 1 μl of 2% WGA-Alexa Fluor 555 (Thermo Fisher Scientific; #W32464) and 0.5% Fast Green (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Spleens were then explanted and placed into a 2 mL Eppendorf tubes filled with 1 mL of RPMI 1640 (Gibco), 50 μM 2-mercaptoethanol (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... with TBST and incubated for 2 hours with horseradish peroxidase coupled secondary goat anti-rabbit or anti-mouse IgG (1:5000, Invitrogen) in TBST ...
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bioRxiv - Immunology 2022Quote: ... The dissociated cells were washed 3 times with chilled PBS containing 1% heat-inactivated FBS and 2 mM EDTA (Gibco).
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bioRxiv - Microbiology 2022Quote: ... Resulting cDNA was diluted 1:2 and used for real-time PCR with Power SYBR Green (Thermo Fisher Scientific 4367659) and Applied Biosystems 7500 real time PCR system ...
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bioRxiv - Neuroscience 2023Quote: ... incubation with primary antisera was followed by 2 hours of incubation with secondary antibody (1:350, goat anti-mouse IgG Alexa Fluor 488; Cat#A1101, Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... pMDG2.1-VSV-G and p8.91-GAG-POL in a ratio of 4:1:2 to a total of 10 μg of DNA using Lipofectamine 2000 (ThermoFisher) as per the manufacturer’s instructions overnight ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed once in PBS and incubated in the following secondary antibody and lipid droplet dye for 2 hr at RT: donkey anti-rabbit 555 (1:500; Invitrogen) and LipidSpot 488 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs were transfected with PB-TO-hNGN2 vector (gift from M. Ward, NIH, Maryland) in a 1:2 ratio (transposase:vector) using Lipofectamine Stem (Thermo Fisher). After 72 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of total RNA was mixed with 2 µl of diluted ERCC RNA spike-in mix (1:100 dilution in nuclease-free H2O; Invitrogen). Ribosomal (r)RNAs were depleted using the Ribominus Eukaryote v2 kit (Ambion) ...
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bioRxiv - Neuroscience 2023Quote: ... and the nuclei pellet was resuspended in 2 mL of ice-cold resuspension buffer (1 M HEPES [#J16924- AE, ThermoFisher Scientific] ...
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bioRxiv - Developmental Biology 2023Quote: ... The 2° antibodies used were Invitrogen™ Alexa Fluor™ Plus 405 Phalloidin (1:100, Fisher Scientific, Cat. No. A30104), Alexa Fluor 647® Goat anti-Mouse IgG2b Cross-Adsorbed Secondary Antibody (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... retinas were incubated on a rocking platform for 2 hours at room temperature (RT) in Claudio’s blocking buffer (CBB) consisting of 1% FBS (LTID 10500-064, Thermo Fisher), 3% BSA (MB04602 ...
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bioRxiv - Cell Biology 2023Quote: Full-length cDNA sequence of Hs WDR23 Isoforms 1 and 2 were cloned into pcDNA 6.2/N-EmGFP/TOPO (Thermo Fisher), as previously described [19] ...
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bioRxiv - Neuroscience 2023Quote: ... was diluted in assay buffer (2 μM in 10 mM HEPES, 150 mM NaCl) containing Sypro Orange (1 in 1000 dilution; Thermofisher). Compounds ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with anti-chicken Alexa Fluor 488 and/or anti-mouse Alexa Fluor 555 secondary antibodies (1:250) for 2 hours before mounting with Prolong Glass (Thermofisher). Confocal z-stacks were obtained by imaging on a Zeiss LSM 700 microscope in the UNC Microscopy Services Laboratory with an EC Plan Neofluar 40x objective using a 1.3 N.A ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted 1:1000 in 2% goat serum/PBS for 1 hour at room temperature and mounted in ProLong Glass mounting media (ThermoFisher). Each slide was then imaged with a Leica Thunder microscope or Zeiss Axio Imager using the Zeiss AxioVision 4.8 software or Leica DM6 B using LASX software ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was DNase digested for 30 min at 37°C using 1 µl TURBO DNase (2 U/µl) (Invitrogen). A 500-ng aliquot of the isolated RNA was converted to cDNA with the iScript Advanced Kit (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: ... differentiation was initiated by switching to DMEM-F12/Neurobasal media (2:1) supplemented with N2 and retinol-free B27 (N2B27 RA−; Gibco). For the first ten days ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed and stained with 1:10,000 DAPI (4=,6-diamidino-2-phenylindole, Thermo Fisher Scientific; catalog #: D3571) for 15 minutes at room temperature before coverslips were applied using PBS + glycerol as mounting medium.
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bioRxiv - Genomics 2023Quote: ... cDNA was synthesized from 1-2 μg total RNA with the Multiscribe High-Capacity cDNA Reverse Transcription Kit (ThermoFisher 4368814).
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bioRxiv - Immunology 2023Quote: ... Cells were then washed with FACS buffer (HBSS with 2% fetal bovine serum) and resuspended in FACS buffer containing propidium iodide (PI; 1:2000; Invitrogen). Panel 1 samples were measured with a FACS Canto II (BD Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were incubated with 1:1000 diluted PE-conjugated F(ab’)2-anti-human-kappa-light-chain (Invitrogen, PA1-74408) in PBS/ 0.1% BSA (20 min ...
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bioRxiv - Immunology 2023Quote: ... resuspended in solubilization buffer (9M Urea containing 1% DTT and 2% Triton X and NuPAGE lithium dodecyl sulfate sample buffer 4X (Invitrogen) at a 3:1 ratio) ...
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bioRxiv - Immunology 2023Quote: ... Antigens were separately diluted down to a concentration of 500nM in 50uL of the 2:1 ratio of RPMI media (Gibco) + FLIPR Calcium 6 dye (Molecular Devices ...
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bioRxiv - Cancer Biology 2024Quote: ... [1-14C]-palmitate (0.1μCi/mL) conjugated to 2% (wt/vol) FA-free BSA and 1 mmol/L -carnitine in RPMI glucose-free medium (Gibco) for 4 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed 3 times in TBS for 10 min each and incubated for 1 h at room temperature with a combination of 2 fluorescent dye-labelled secondary antibodies: a Cy3-conjugated goat anti-rabbit antibody (1:200; Invitrogen, Waltham ...
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bioRxiv - Neuroscience 2022Quote: ... counterstained with 1 μg/mL diamidino-2-phenylindole dihydrochloride (DAPI) and cover-slipped with ProLong Glass antifade (Life Technologies #P36980) before imaging ...
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bioRxiv - Cell Biology 2022Quote: ... and control cell lines were obtained by transduction with retroviral particles generated in Hek293T cells and sustained selection with puromycin 2 μg ml-1 (A1113803, ThermoFisher). Specifically ...
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bioRxiv - Neuroscience 2022Quote: ... Maturation medium I was DMEM/F12 supplemented with 100 U/ml Penicillin-Streptomycin and 1% N-2 Supplement (Gibco, 17502048). On day 10 ...
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bioRxiv - Physiology 2022Quote: ... 2×105 CD11b+ monocytic BM cells that were isolated by biotinylated anti-CD11b (1:100; clone M1/70; ThermoFisher Scientific) and anti-biotin microbeads (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1×106 cells were resuspended in 500μl FACS buffer (PBS with 2% fetal bovine serum (Gibco, 10099-141, lot 1751896)) ...
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bioRxiv - Immunology 2022Quote: ... Purified DEL was conjugated to biotin at a 1:2 molar ratio using Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific) according to the manufacturer’s directions and incubated for 3 hours on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was generated from 1-2 mg of total RNA using random hexamers and Maxima Reverse Transcriptase (Thermo Fisher Scientific). Quantitative PCR was performed on the ViiA 7 Real-Time PCR System (Life Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... polyethyleneimine (PEI) was used as a transfection reagent and HEK293T cells were incubated with DNA:PEI (1:2) in OPTIMEM (Life Technologies) for two hours ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were centrifuged at 500 ×g for 5 minutes at 4°C and washed and resuspended with ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS) ...
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bioRxiv - Cell Biology 2024Quote: Synchronized day 1 adult worm populations were collected in M9 Buffer and then diluted with 2× LDS Sample Buffer (Invitrogen). The samples were subjected to a controlled heat treatment to induce disorder in protein conformations ...
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bioRxiv - Cell Biology 2024Quote: ... both at 1:300 dilution and mounted with ProLong Gold antifade reagent with DAPI (4’,6-diamidino-2-phenylindole; Invitrogen). Confocal microscopy (Olympus FLUOVIEW FV3000 ...
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bioRxiv - Microbiology 2024Quote: Phage DNA was isolated by treating 2 ml of Phage solution with 1 U/μL DNAse (Invitrogen, Carlsbad, CA, USA) to remove free DNA ...
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bioRxiv - Microbiology 2024Quote: ... and differentiated using 2.5% FBS neurobasal media (1X B27, 20 mM KCl, 1% Pen-Strep, 2 mM Glutamax (Thermo Fisher) 50 ng/mL Brain-derived neurotrophic factor (BDNF) ...
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bioRxiv - Immunology 2024Quote: ... IgGs from individual serum or pooled sera bound to the plates were detected after a 2-h incubation with goat anti– mouse-IgG–linked horseradish peroxidase (1:8,000 Invitrogen, USA). For measuring IgG isotypes ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed x5 with PBS for 5 min and incubated for 2 hr at room temperature with 1:1000 Alexa Fluor 568 donkey anti-goat antibody (Invitrogen) dissolved in PBS containing 0.5% Triton X-100 and 3% normal donkey serum.
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bioRxiv - Immunology 2023Quote: ... The appropriate volume of cells was then pelleted at 1500rpm for 5 minutes after which the supernatant was decanted and the cells were resuspended at a 2:1 ratio of RPMI media (Gibco) + FLIPR Calcium 6 dye (Molecular Devices) ...
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bioRxiv - Developmental Biology 2024Quote: ... goat-anti mouse Alexa Fluor 488 or 568) combined with 2 drops of NucBlue in 1 ml PBT (Invitrogen, RF37606), for 1-2 days ...
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bioRxiv - Developmental Biology 2024Quote: ... column and the sample chamber was filled with E3 supplemented with 0.2 mM 1-phenyl-2-thiourea (10107703, Acros Organics) and 0.04% tricaine methanesulfonate (MS-222 ...
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bioRxiv - Cell Biology 2023Quote: ... ApoER2 plasmids were co-transfected with RAP plasmid at a 1:2 ratio in Opti-MEM medium (Gibco, Thermo Fisher) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... ApoER2 plasmids were co-transfected with RAP plasmid at a 1:2 ratio in Opti-MEM medium (Gibco, Thermo Fisher) using Lipofectamine 2000 (Invitrogen) ...