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Citations for Thermo Fisher :
6901 - 6950 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 2 μg of total RNA harvested from parental RPE-1 and D12 cells utilizing the TRIzol reagent (ThermoFisher Scientific, 15596026) was used for cDNA synthesis (Applied Biosystems ...
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bioRxiv - Genetics 2023Quote: Samples were then incubated at 37 °C for 1 h after addition of 2 μl of RNase Cocktail Enzyme Mix (Ambion). Then 40 μg of Proteinase K (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... The filtrate was then incubated for 1 h rotating at 4 °C with 2 mL Ni-NTA resin (Thermo Fisher) per 1.5 L of expression culture ...
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bioRxiv - Developmental Biology 2023Quote: ... The mRNA from plasmid #1 and #2 was transcribed using the mMESSAGE mMACHINE SP6 Transcription Kit (Thermo Fisher Scientific, AM1340) and purified with the NucleoSpin RNA Clean-up XS kit (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA and RNA products at various points of the iTP-seq protocol were analyzed on 9% acrylamide (19:1) TBE (90 mM Tris, 90 mM boric acid, and 2 mM EDTA) gels and stained with SyBR Gold (Invitrogen). Inverse toeprints were excised from 12% acrylamide TBE gels using a clean scalpel ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 h with secondary antibodies diluted in blocking buffer to 1:600 (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen, Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed with assay buffer (PBS containing 0.2% BSA and 1 mM CaCl2) and resuspended in assay buffer with 2 μM Fluo-4-AM (Life Technologies). Fluo-4-AM was prepared as a 1 mM stock in DMSO ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Differentiation was initiated by changing media to N2B27-RA media (2:1 ratio of DMEM/F12 and Neurobasal medium (Gibco), +1% L-glutamine ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for cells cultures or 2% (v/v) SVF for parasites cultures (Biowest, France) and supplemented with 1% antibiotics (penicillin-streptomycin) (GIBCO).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and perilipin-2 was stained with Alexa Fluor™ 488 goat anti-rabbit IgG (1:500, # 11034, Lot: 10729174; Invitrogen) for 30 min (RT).
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... and after 24-hour the medium was replaced with serum-free advanced DMEM supplemented with 2 mM L-glutamine and 1% antibiotic-antimycotic (Gibco). After incubation for 48 hours ...
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bioRxiv - Genetics 2024Quote: ... tissue samples were incubated for 2 h with 1 ml of an antibiotic solution containing Gentamicin (50 µg/ml Gibco) and PrimocinTM (50 µg/ml ...
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bioRxiv - Immunology 2024Quote: ... anti-mouse and anti-rabbit secondary antibodies was applied with DAPI nuclear stain (4’6-diamidino-2-phenylindole, 1:500, #62248, ThermoFisher Scientific). All reagents were prepared in multistaining buffer (BU06 ...
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bioRxiv - Developmental Biology 2024Quote: ... sections or cells were incubated with bisbenzimide (1:100 in 0.1M PB) for 2 min at room temperature and mounted using Fluoromount-G (Thermo Fisher).
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bioRxiv - Cell Biology 2024Quote: ... Egg chambers were washed 3X 20 min in PBST and fixed in 4% PFA followed by 3X 10 min washes in PBST preceding a 2 hr incubation in a fluorescently labeled secondary (1:1000; Dylight 550 or 650; ThermoFisher). Washed 3X for 10 min in PBST and mounted as previously described ...
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bioRxiv - Cell Biology 2024Quote: ... Switzerland) and Hoechst 33342 dye (1 drop/2 ml of NucBlue Live ReadyProbes Reagent, Thermo Fisher Scientific, Waltham, MA, USA) and have been confirmed to be mycoplasma free.
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bioRxiv - Molecular Biology 2024Quote: ... Roughly 2 mg of purified Tfb3 was covalently linked to 1 ml AminoLink® Plus Coupling Resin (Thermo Scientific #PI20505) following the manufacturer’s protocol for crosslinking at pH 10 with cyanoborohydride solution ...
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bioRxiv - Neuroscience 2024Quote: ... in a solution of PBS with 0.2% Triton–X100 and tissue sections were incubated with goat anti-eYFP (Anti-GFP chicken IgY fraction 1:1000, Invitrogen) and anti-c-Fos (Anti-c- Fos rabbit ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were imaged in non-fluorescent culture medium (FluoroBrite 4.5 g/l Glucose, 1% PenStrep, 2% FBS, L-Glutamine from Life Technologies). For secretion and capture ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... and the medium was then replaced with serum-free advanced DMEM supplemented with 2 mM L-glutamine and 1% antibiotic-antimycotic (Gibco). After incubation for 48 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... were cultured in complete DMEM (10% Tetracycline free FBS, 1% Penicillin-Streptomycin) including 2 mg/ml G418 (Generon) and 50ug/ml hygromycin (ThermoFisher). All cell lines were screened every three months for mycoplasma infection.
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bioRxiv - Neuroscience 2024Quote: ... retinas were incubated with secondary antibodies at 4°C for another 2-3 days: goat anti-chicken antibody conjugated to Alexa Fluor 488 (1:1000; A11039, Invitrogen), goat anti-rabbit 555 (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in RDM to OD600 of ≈0.03 and several 1-µl drops of diluted cell suspension were placed on an agarose pad prepared with RDM and 2% agarose containing 1 µM SYTOX Blue (Invitrogen) for staining of dead cells ...
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bioRxiv - Molecular Biology 2024Quote: ... and run at 120V for 1-2 hours in NuPAGE MES SDS Running Buffer or NuPAGE MOPS SDS running buffer (Invitrogen) with 250μL NuPAGE antioxidant (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... for 2 hours followed by secondary staining for 1h with Alexa Fluor anti-rabbit-647 (Life Technologies, 1:500 dilution), Alexa Fluor antimouse-594 (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... for 15 min three times and incubated at room temperature for 2 hours with corresponding secondary antibodies including: donkey anti goat 568 (1:500, A-11057, Invitrogen), donkey anti rat 647 (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... For buffered growth mDMEM-DMP was supplemented with 100 mM of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco 15630056).
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bioRxiv - Microbiology 2024Quote: ... before being washed and incubated with goat anti-mouse Alexa-Fluor 750 (1:100, 2 hours at 25°C, catalog no. A21037; Invitrogen) for 2 hours at 25°C ...
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bioRxiv - Immunology 2024Quote: ... Fisher)) and blocked with PBS with 1% BSA (w/v) and 2% goat serum diluted in PBS (Gibco; v/v) for 1hour at 22-25°C ...
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bioRxiv - Immunology 2024Quote: Immunogens expressed with a free terminal cysteine were reduced at 1 mg/mL with 10 molar equivalents of tris(2-carboxyethyl)phosphine (TCEP, ThermoFisher) in TBS and incubated at 25°C for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... resuspended in solubilization buffer (9M Urea containing 1% DTT and 2% Triton X and NuPAGE lithium dodecyl sulfate sample buffer 4X (Invitrogen) at a 3:1 ratio) ...
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bioRxiv - Genomics 2024Quote: ... 1-2 million cells were washed in 1X chilled PBS and stained with live/dead staining dye (L34976-Life Technologies) as per the manufacturer’s staining recommendations ...
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bioRxiv - Microbiology 2024Quote: ... We treated them with a 1:1 mixture of conditioned medium and normal culture medium (DMEM with high glucose [Sigma], 2% fetal bovine serum [ThermoFisher Scientific], 1% MEM NEAA [ThermoFisher Scientific] ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed 1 time with complete medium and cultured in complete medium containing 2 µg/mL puromycin (Gibco) at a final volume of 200 µL per well in flat-bottom plates and maintained at 37°C under an atmosphere of 5% CO2 for 24 hours ...
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bioRxiv - Microbiology 2024Quote: ... 1,000,000 EGI-1 or HepG2 cells were plated in 2 mL complete DMEM media in 6-well Nunclon Delta-treated plates (ThermoFisher) and incubated at 37 °C with 5% CO2 overnight ...
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bioRxiv - Microbiology 2024Quote: ... Mutated heavy and corresponding light chain genes were cloned into appropriate expression plasmids for eukaryotic cell expression and were co-transfected at a 2:1 ration into FreeStyle 293-F cells (ThermoFisher). Fabs were purified using KappaSelect affinity chromatography (Cytiva).
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bioRxiv - Physiology 2024Quote: ... hearts were loaded with 1 mg/ml Rhod-2 AM in 0.5 mL of DMSO containing 10% pluronic acid (Molecular Probes) followed by 50 µL 1 mg/ml RH237 in DMSO (Molecular Probes) ...
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bioRxiv - Immunology 2024Quote: ... Plasmids encoding antibody heavy and light chain genes were used to transfect suspension Expi293F cells in a 1:2 heavy to light chain ratio using ExpiFectamine 293 transfection reagents (Gibco) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... DRGs cultures were grown at 37 °C in Neurobasal-A medium supplemented with 2% B-27 and 1% Glutamax-I (Gibco).
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bioRxiv - Microbiology 2024Quote: ... 1% hematocrit) were resuspended in binding medium (RPMI 1640 medium, 2% glucose, pH 7.2; Gibco, Thermo Fisher Scientific, Bremen, Germany) and co-incubated with HBEC-5i cells (70-90% confluence ...
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bioRxiv - Neuroscience 2024Quote: ... PolyA+ RNA-seq libraries were prepared from 500 ng total RNA input supplemented with 2 μl 1:200 ERCC RNA Spike-In Mix (Invitrogen) using the TruSeq Stranded mRNA HT Kit (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were washed 6 times with PBS for 2 min and incubated with Alexa Fluor 568- (1:500 dilution, Invitrogen), Alexa Fluor 488- (1:500 dilution ...
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bioRxiv - Molecular Biology 2024Quote: ... All samples were then incubated with DAPI (4’,6’-diamidino-2-phenylindole) at 1 ug/ml in PBS to visualize DNA and mounted in Prolong Gold mounting media (Invitrogen). Images were acquired on the Zeiss LSM 980 microscope.
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bioRxiv - Developmental Biology 2024Quote: ... for 2 hrs at RT and incubated overnight at 4°C in primary antibody solution (1:100 rabbit anti-GFP (ThermoFisher) in blocking reagent) ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... ExpiCHO cells were transiently transfected with the vector containing the gene encoding soluble spike glycoprotein ectodomain or CR3022 (using a heavy chain:light chain ratio of 1:2) using the Expifectamine 293 Transfection Kit (ThermoFisher #A29129), following the user manual ...
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bioRxiv - Microbiology 2024Quote: ... blocked with 2% BSA in a PBS buffer for 10 min and incubated with 0.1–1 μg/ml DAPI (4’, 6-diamidyno-2-fenyloindol, Molecular Probes) and WGA-Texas Red (Wheat Germ Agglutinin-Texas Red ...
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bioRxiv - Synthetic Biology 2024Quote: ... inserts were fabricated using 3D-printed resin molds and 1 (or 2) mm biopsy punches (Fisher Scientific #12-460-402). Printed molds were washed with isopropanol after printing ...
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bioRxiv - Microbiology 2024Quote: ... 750 mM aminocaproic acid, 2 mg/ml lysozyme, 1 mM EDTA, 1.43 mM BME, 1x EDTA-free Pierce Protease Inhibitor [Thermo Scientific]) and incubated 20 min on ice ...
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bioRxiv - Neuroscience 2024Quote: ... before incubation with DAPI (4’,6-diamidino-2-phenylindole, dilactate, 0.1 µg ml−1 final concentration in PB, Invitrogen D3571) in 0.1 M PB for 10–20 mins at RT ...