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Citations for Thermo Fisher :
6651 - 6700 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 minutes prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... and HeLa (ATCC CCL-2) cells were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2021Quote: ... by heating at 95 °C for 5 min with 2 µL of SDS-PAGE loading dye (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation flow cytometry assays were performed using the 5-ethynyl-2’deoxyuridine (EdU) assay kit (Invitrogen #C10634). Cells were incubated with EdU for 24 h and labeled ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were treated with 10 μM of Edu (5-ethynyl-2′-deoxyuridine; Invitrogen™, Thermo Fisher Scientific, USA), and incubated for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were treated with 10 μM of Edu (5-ethynyl-2′-deoxyuridine; Invitrogen™, Thermo Fisher Scientific, USA), and incubated for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped by adding cold blocking buffer (5 mM EDTA, 2% fetal bovine serum (FBS) (Gibco) in HBSS) ...
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bioRxiv - Neuroscience 2024Quote: ... each animal was injected intraperitoneally with 37 mg/kg BW of 5-ethynyl-2′-deoxyuridine (EdU, ThermoFisher, A10044) at 48 hours after surgery ...
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bioRxiv - Neuroscience 2024Quote: ... each animal was injected intraperitoneally with 37 mg/kg BW of 5-ethynyl-2′-deoxyuridine (EdU, ThermoFisher, A10044) at 48 hours after surgery ...
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bioRxiv - Cell Biology 2024Quote: ... The seeded cells were allowed to attach overnight prior to transfection with plasmid DNAs (0.1-0.2 μg/well) using Lipofectamine 2000 (2-5 μL/well; Invitrogen) within a small volume of Opti-MEM (200 μL ...
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bioRxiv - Immunology 2024Quote: ... or BA.5 (2 μg/mL) spike proteins were coated onto 96-well Maxisorp plates (ThermoFisher, Cat. #439454) in 50 μL of 50 mM Na2CO3 pH 9.6 overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4–12% Bis-Tris gel (Thermo Fisher Scientific Cat#NP0335BOX) using MES running buffer (Thermo Fisher Scientific Cat#NP000202) ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with PBS and incubated 5 minutes with PBS containing 2 µg/mL Hoechst (33342, Fisher Scientific, UK) and washed in PBS and briefly in ddH2O then mounted on glass slides using ProLong GoldTM anti-fade glass mounting medium (P36980 ...
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bioRxiv - Microbiology 2022Quote: ... and an Acclaim PepMap 100 trap column (100 μm × 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 5 min with 0.05 M PB and mounted on Superfrost Plus (ThermoFisher Catalog# 12-550-15) microscope slides ...
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bioRxiv - Neuroscience 2023Quote: ... in plating media (Neurobasal, Gibco; supplemented with 5% horse serum, Sigma; 2 mM GlutaMAX, Gibco; and 1xGS21, GlobalStem). 500,000 cortical neurons were plated per well of a 6 well plate pre-coated with PDL ...
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bioRxiv - Genetics 2023Quote: ... 3-5 million cells or approximately 15 ug of DNA crosslinked with 2% PFA (Fisher Scientific F79-500) were used as input per reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5% 2-mercaptoethanol] and was resolved by SDS-PAGE on 4% to 12% Bis-Tris gels (Invitrogen). Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 min prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Biochemistry 2022Quote: ... 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher) through gold coated capillary needles that were prepared in-house30 ...
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bioRxiv - Cell Biology 2023Quote: Embryos were incubated for 1hr at 28ºC with 400 µM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen Cat# A5040) and 5% DMSO in E3/PTU ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM disuccinimidyl suberate (DSS) or 2 mM tert-butyl disuccinimidyl phenyl phosphonate (tBu-PhoX)(Thermo Fisher Scientific) in DMSO was added to the solution and incubated for 1 hr at room temperature ...
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bioRxiv - Genetics 2023Quote: ... Cells were then blocked with 2 mL blocking solution made of 5% Normal Goat Serum (cat#PCN5000 Thermofisher) and 0.05% Triton X-100 in PBS for 2 hours at RT or 4°C on a shaker.
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bioRxiv - Biochemistry 2024Quote: ... Cells were allowed to attach overnight prior to transfection with plasmid DNAs (0.5-0.75 μg/well) using Lipofectamine 2000 (2-5 μL/well; Invitrogen). Lipofection was done using small volumes of Opti-MEM (200 μL ...
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bioRxiv - Immunology 2024Quote: ... we injected the mice intraperitoneally with 4 mg/ml of 5-ethynyl-2′-deoxyuridine (EdU) (Invitrogen, Waltham, MA) with the goal of labeling circulating monocytes to assess macrophage retention among groups ...
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bioRxiv - Systems Biology 2024Quote: ... followed by reduction and alkylation in 5 mM tris(2 - carboxyethyl)phosphine (TCEP) (Thermo Fisher, Cat No. 77720) and 50 mM 2-Chloroacetamide (CAA ...
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bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4–12% Bis-Tris gel (Thermo Fisher Scientific Cat#NP0335BOX) using MES running buffer (Thermo Fisher Scientific Cat#NP000202) ...
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bioRxiv - Cell Biology 2024Quote: ... and then nutated in Grace’s Insect Medium containing 0.25 mg/ml 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen A10044) for 30 min at room temperature to label S-phase cells ...
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bioRxiv - Cancer Biology 2024Quote: ... 2-5 mg of minced tissue was loaded into a cuvette containing Amplex Red (Invitrogen, Waltham, MA, USA), horseradish peroxidase (5U/mL) ...
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bioRxiv - Bioengineering 2022Quote: ... was incubated for 2h at room temperature at 1:500 together with alexa-coupled wheat germ agglutinin (WGA, 1:500, Thermofisher). Sections were mounted in DAPI Fluoromount-G (SouthernBiotech ...
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bioRxiv - Molecular Biology 2022Quote: ... x 5 mm 5 μm 100 Å and an Acclaim PepMap RSLC 75 μm x 50 cm 2 μm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Dionex) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µL of 5 mM aa-dUTP/dNTP mix (5 mM each dATP, dGTP and dCTP, 3 mM dTTP and 2 mM 5-(3-aminoallyl)-dUTP (Thermo Fisher Scientific, AM8439)) and 1.25 µL of 40 µM oligo 1 ...
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bioRxiv - Microbiology 2024Quote: Total RNA extracted from BoDV-2-infected Vero cells was ligated with either 5’ adaptor oligoRNA (5’-CGACUGGAGCACGAGGACACUGACAUGGACUGAAGGAGUAGAAA (Thermo Fisher Scientific, USA; #L150201)) or 3’ adaptor oligoRNA (5’-GAAGAGAAGGUGGAAAUGGCGUUUUGG ...
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bioRxiv - Biochemistry 2024Quote: ... x 5 mm 5 μm 100 Å and an Acclaim PepMap RSLC 75 μm x 50 cm 2 μm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... Four-day-old seedlings grown under constant white light (70 μmol s−1 m−2) were transferred to 96-well plates (136101 Nunc MicroWell White plate ...
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bioRxiv - Plant Biology 2020Quote: ... After 24 h illumination cells were resuspended and re-plated onto f/2-NH4 agar plates containing 80 μg mL−1 zeocin (Invitrogen). The plates were maintained under illumination for 3 weeks and the resistant colonies were screened by PCR for the presence of SYN genes as previously described (Falciatore et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... and resuspended in water to 0.5–2 μg μl–1 measured by light absorbance on a NanoDrop spectrophotometer (Thermo Fisher), as previously described11,12.
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bioRxiv - Cancer Biology 2021Quote: ... targeting PARP1 (sequence 1: #HSS100243 and sequence 2: #HSS100244) or a scrambled (SCR) control sequence (sequence #12935300) with oligofectamine (Invitrogen), according to the manufacturer’s recommendations.
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bioRxiv - Biochemistry 2021Quote: ... RNA was precipitated overnight at −20°C by the addition of 1 volume of isopropanol and 2 μL of GlycoBlue (15 mg/mL, Invitrogen). RNA was pelleted by centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... Gel extraction buffer was added to 1 mL ice-cold 100% ethanol and 2 μL of glycogen (20 mg/mL, ThermoFisher) and incubated at −20°C for at least one hour ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were transfected with 40nM siRNA (siControl; silencer siRNA negative control Cat. #4390843, siSrsf3 #1; s73613, siSrsf3 #2; s73615, ThermoFisher) for 48h at 33°C using Lipofectamine RNAiMAX Transfection Procedure (ThermoFisher Cat.#13778-100).
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bioRxiv - Cell Biology 2020Quote: ... The mixture was then washed with sorting buffer (2% FBS and 1% Penicillin-Streptomycin in DMEM (Cat#31053028; Thermo Fisher)) ...
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bioRxiv - Genetics 2021Quote: ... animals were resuspended in 200 µl of M9 and 1 µl of 2 mg/ml DiI (Molecular Probes, Carlsbad, CA) in dimethylformamide (DMF ...
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bioRxiv - Biophysics 2020Quote: ... lymph-node derived cells were mixed in a 1:2 ratio with OVA-peptide pulsed spleenocytes and cultured in RPMI 1640 culture medium (Gibco) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Immunology 2022Quote: ... Organoids were then washed with PBS and then incubated for 1-2 h at room temperature with Alexa Fluor-conjugated secondary antibodies (Invitrogen). Organoids were washed again with PBS and mounted in Vectashield (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated with secondary antibodies in vehicle for 2 h at room temperature (goat-anti-rabbit Alexa 546, 1:500, ThermoFisher A-11035 ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: ... Samples were then incubated for 2 h with anti-rabbit Alexa Fluor 594 secondary antibody (1:500; Thermo Fisher Scientific). Slices were washed and mounted on microscope slides ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed in PBS and incubated (2 h) with secondary antibodies anti-rabbit or anti-mouse Alexa488 (1:1000, Invitrogen), and/or anti-rabbit or anti-mouse CY3 (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Detection of SARS-CoV-2 N1 gene sequences and host RNase P mRNA from de-identified SARS-CoV-2 positive samples was carried out with the one-step TaqMan RNA-to-Ct 1-Step Kit (ThermoFisher), using 2 μL of undiluted PEARL extract ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was then washed with PB and incubated overnight in PBT-X (2% NHS, 0.2% TritonX-100 in PB) with secondary antibody (1:1000 AlexaFluor 488-conjugate anti-rabbit, ThermoFisher Scientific). Tissue was washed with PB and mounted onto gelatinised slides ...