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Citations for Thermo Fisher :
6951 - 7000 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The slides were washed in incubation buffer 6x5 mins and incubated in secondary antibodies for another 2 hours at room temperature (Goat anti-Chicken-IgY antibody, Alexa-488, 1:2000, Cat# A11039, ThermoFisher; Goat anti-Rabbit-IgG antibody ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 2 hours at room temperature with Alexa fluor 488 and Alexa Fluor 633 conjugated goat secondary antibody (1:400, Invitrogen). Nuclei were detected by using Hoechst 33528 (1ug/ml ...
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bioRxiv - Synthetic Biology 2024Quote: Flow cytometry samples containing cells in 1x PBS (with 2 mg mL-1 of kanamycin) were analysed using the Attune NxT flow cytometer (Thermofisher) equipped with a Cytkick autosampler ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed 3×2 minutes with DPBS before a permeabilization solution (0.1% Triton-X (Fisher Scientific, 9002-93-1) in DPBS ...
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bioRxiv - Neuroscience 2024Quote: ... were co-transfected with EGFP-N1 in a proportion 2:1, by in-tube transfection as described previously (Halterman et al., 2009) with Lipofectamine 2000 (ThermoFisher). For neurite analysis ...
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bioRxiv - Microbiology 2024Quote: ... Cells were stained with 2 ml of antibody mixture diluted in Brilliant Stain Buffer (Table 1; Invitrogen #00-4409-42) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... from day 1-2 Sprague-Dawley rats and maintained in DMEM (L-glutamine, Sodium Pyruvate, Non-essential amino acids; Gibco) supplemented with 2% FBS (Gibco ...
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bioRxiv - Immunology 2024Quote: ... and transfected with either the D614G Wuhan-Hu-1 or Omicron BA.2 S constructs using Lipofectamine 2000 (Life Technologies). After 4–6 h ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were then incubated for 1 hour in 2-3 drops per slide of HRP-conjugated streptavidin (Alexa Fluor 594 Tyramide Superboost Kit, Invitrogen), washed 3 times for 10 minutes each in 1x PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Click reaction was used to detect EdU labelling by incubating the cells with a click buffer (100 mM Tris-HCl pH8; 2 mM CuSO4; 1 M Alexa488-Azide – Invitrogen AI0266 ...
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bioRxiv - Genomics 2024Quote: ... NRVM pellet was then resuspended in 2 mL of DMEM with 10% FBS and 1% pen/strep and counted using a Countess II cell counter (ThermoFisher). Cells were plated onto gelatin-coated 12-well plates at a density of 200-250k cells per well.
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bioRxiv - Microbiology 2024Quote: ... Part of the RBC pellet of the clinical malaria case were in vitro cultured at 1-2 % haematocrit in RPMI 1640 (Gibco) complete medium (with L-glutamine and HEPES) ...
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bioRxiv - Genomics 2024Quote: ... Samples were then incubated at 37 °C for 1 h after addition of 2 μl of RNase Cocktail Enzyme Mix (Ambion). Then 40 μg of Proteinase K (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... floating sections were incubated for 2 h at room temperature with the goat anti-mouse Alexa 488-labeled secondary antibody (1:500, Invitrogen). To visualize cell nuclei ...
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bioRxiv - Bioengineering 2024Quote: ... samples were washed again with PBST and incubated with 4′,6-diamidino-2-phenylindole (DAPI, Molecular Probes, 1:2000 dilution) and phalloidin-tetramethyl rhodamine B isothiocyanate (phalloidin-TRITC ...
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bioRxiv - Plant Biology 2024Quote: ... Tak-2 and Mpmri-1 thalli grown on cellophane membranes to preserve the rhizoids using TRI reagent (Ambion Life Technologies). Two μg of DNaseI-treated ...
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bioRxiv - Plant Biology 2024Quote: ... Tak-2 and Mpmri-1 thalli grown on cellophane membranes to preserve the rhizoids using TRI reagent (Ambion Life Technologies). Two μg of DNaseI-treated ...
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bioRxiv - Cell Biology 2024Quote: ... Egg chambers were washed 3 x 10 min followed by 2 hr incubation in a fluorescently labeled secondary antibody (1:1000; DyLight 550 or DyLight 650; ThermoFisher). Egg chambers were washed 3 x 20 min in PBST and were fixed again in 2% PFA ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mL of cell culture media with 10 µM thapsigargin and 2 drops of NucBlue live cell stain (Life Technologies) were added immediately after beadloading to keep inducing U-bodies and to stain the nucleus ...
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bioRxiv - Bioengineering 2024Quote: ... cells were stained with 2 μg ml−1 of the cell-permeable DNA dye Hoechst 33342 (Thermo Fisher Scientific, 62249) for 10 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: 2E4 cells were seeded in 24-well microplates and incubated overnight prior to the addition of 2 μg/1E6 cells of HPV16 VLPs conjugated to pHrodo (10:1 dye:HPV L1 ratio) (ThermoFisher Scientific). pHrodo labelled particles were generated using the manufacturer’s protocol and were purified with 2% agarose beads (sized 50-150 μm ...
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bioRxiv - Cell Biology 2024Quote: ... Switzerland) and Hoechst 33342 dye (1 drop/2 ml of NucBlue Live ReadyProbes Reagent, Thermo Fisher Scientific, Waltham, MA, USA) and have been confirmed to be mycoplasma free.
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bioRxiv - Developmental Biology 2024Quote: ... Pan-retinal (PAN) differentiation medium contained SFM + 20ng/μL bFGF + 2 ng/μL heparin + 1% heat inactivated FBS (Gibco, 10082147), COCO differentiation medium was PAN medium + 50 ng/μL COCO (R&D ...
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bioRxiv - Neuroscience 2022Quote: ... Peptides were washed two times with 200 µl 1% TFA 99% ethyl acetate (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... and EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... 2h) conjugated to AF-568 (1:500 goat-anti-rabbit, Life Technologies #A11011; RRID: AB_143157) or AF-647 (1:500 goat-anti-mouse ...
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bioRxiv - Developmental Biology 2020Quote: ... After blocking for 2h at RT in CAS-Block (1:10 in PBST; ThermoFisher, #008120) blocking reagent was replaced by antibody solution (diluted in CAS-Block ...
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bioRxiv - Molecular Biology 2022Quote: ... Sections were washed thrice with 1× PBS then stained with the secondary antibody goat anti-mouse Alexa Fluor® 555 Plus conjugate (2 μg ml−1, 1:1000-fold dilution; Cat No. A32727, Molecular Probes, ThermoFisher, Waltham, MA, USA) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Samples were washed with PBS-T three times (flush×1 and 10 min×2) and stained with Donkey anti-rat IgG Alexa Fluor 488 (Thermo Fisher Scientific, A21208, 1:1000 dilution) and Hoechst 33342 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed five times in 1% BSA (in PBST) before staining with Alexa Fluor 568-conjugated secondary antibody (Invitrogen, A11036, 1:500 in 2% BSA) for 1.5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... 2ml serum free DMEM or 2) 2 mL Cell Mask Orange (CMO: 2.5 µg/mL in serum free DMEM) (Thermofisher Scientific cat C10045) and both were incubated at 37° C for 10 minutes.
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bioRxiv - Neuroscience 2020Quote: ... Primary human dermal fibroblasts were collected from VWMD patients (2) or control family members (2) and maintained in DMEM/F12 (Life Technologies, 21331020), 10% FBS (Bovogen ...
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bioRxiv - Synthetic Biology 2021Quote: ... Supernatant was harvested at the indicated timepoints and IL-2 levels in the supernatant were measured via IL-2 Human Instant ELISA kit (Thermo Fisher #BMS221INST). T-cell proliferation was also measured at the indicated timepoints using a BD FACSymphony Fortessa X-50.
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bioRxiv - Plant Biology 2020Quote: ... cinerea germlings exposed to different concentrations NCR044.1 was measured every 30 min for 2 h using the ROS indicator dye 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA, Invitrogen, Carlsbad, CA). ROS levels were quantified by measuring the fluorescence in a SpectraMax® M3 spectrophotometer microplate reader (exc ...
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bioRxiv - Neuroscience 2022Quote: ... 2.8×106 neural progenitor cells were grown on glass coverslips in 6-well plate and loaded with 2 µM Fura-2/AM and Pluronic F127 in OptiMEM (Thermo Fisher Scientific) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: 3 μl of purified ribosome sample was applied to Quantifoil 300 mesh R2/2 grids with an ultrathin 2 nm carbon layer and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) at 15 °C ...
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bioRxiv - Biophysics 2022Quote: ... The sample was diluted to 0.5 mg/ml and directly applied to freshly glow-discharged 2/2 Cu Quantifoil grids in a Vitrobot Mark IV (Thermo Fisher Scientific) at 4° C and 100% humidity ...
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bioRxiv - Microbiology 2020Quote: ... Tryptic peptides (2 μg) were loaded onto a C18 trap (75 um x 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Fisher Scientific) at a flow rate of 2 ul/min of solvent A (0.1% FA and 3% acetonitrile in LC-MS grade water) ...
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bioRxiv - Microbiology 2021Quote: ... incubated in primary antibody (anti-SARS-CoV-2 Spike, which detects the S2 subunit of SARS-CoV-2 S (Invitrogen, PA1-41165), anti-GAPDH (proteintech ...
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bioRxiv - Neuroscience 2020Quote: ... sequences were amplified from plasmids derived from ser-2(2)p::FRT::YFP,andclonedupstreamof FRT::STOP::FRT::GCaMP6s to generate WY016 using Gateway (Thermo Fisher Scientific). To generate PSAB1206 ...
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bioRxiv - Cancer Biology 2022Quote: ... was diluted in T cell medium (RPMI 1640 + 10%FBS + L-glutamine (2 mM) + penicillin (100 U/ml + streptomycin (100 μg/ml) + 55 μM 2-Mercaptoethanol (Thermo Fisher, #21985023)) ...
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bioRxiv - Neuroscience 2020Quote: ... Some slices (used for experiments in Fig. 2 and 3) were cultured in media that included 2 % B-27 Supplement (Thermo Fisher Scientific). Slices were maintained at 37°C and 5 % CO2 and used for experiments at DIV10-15.
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bioRxiv - Microbiology 2021Quote: ... incubated in primary antibody (anti-SARS-CoV-2 Spike, which detects the S2 subunit of SARS-CoV-2 S (Invitrogen, PA1-41165), anti-GAPDH (proteintech ...
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bioRxiv - Cell Biology 2021Quote: Amnion and choriodecidua explants (2 cm2) were placed in six-well plates with 2 ml of DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: Amnion and choriodecidua explants (2 cm2) were placed in six-well plates with 2 ml of DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... scrambled or PUS1/PUS7ko HEK-293Ts were transfected with 2 μg PAN and 2 μg ORF57-eGFP plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and rested for 30 minutes at room temperature in a buffer consisting of 2% FBS in 1X PBS (2% FACS buffer) with 50nM dasatinib (Thermo Fisher Scientific). Cells were washed with 2% FACS buffer with 50nM dasatinib and incubated with the Gag386-394GW9 tetramer for 45 minutes at room temperature ...
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Plastid double-strand RNA transgenes trigger small RNA-based gene silencing of nuclear-encoded genesbioRxiv - Plant Biology 2022Quote: ... The gel was imaged and washed 2 x 15 minutes in nuclease-free water then 2 x 15 minutes in 10X UltraPure SSC Buffer (Invitrogen, Carlsbad, CA) with gentle shaking ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa (CRM-CCL-2) and U-2 OS (HTB-96) cells obtained from ATCC were cultured in DMEM (Thermo Fisher Scientific, 12800017) supplemented with 10% fetal bovine serum (Lonsera ...
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bioRxiv - Biochemistry 2023Quote: ... To initiate reaction, 200 µL of Assay Buffer with substrate mixture (30 mM K3PO4, 2 mM MgSO4, 2 mM DTT (Fisher Scientific, AAJ1539706), 0.56 mM TPP (Sigma-Aldrich ...