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Citations for Thermo Fisher :
6851 - 6900 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 20 ng/mL TPO and 40 ng/mL IL-3 from day 1 to day 6 with a media refresh every 2 days (all cytokines from Thermofisher). On day 8 ...
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bioRxiv - Cell Biology 2024Quote: ... the DNA-lipid complex (3 μg linearized Rosa26 targeting vectors : 2 μg pX459-sfCherry-sgRosa26-1 : 7.5 μL Lipofectamine 3000 in 250 μL Opti-MEM; Thermo Fisher) was added to 1.62×105 cells as they were seeding onto a 35 mm dish with 2 mL of growth medium ...
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bioRxiv - Developmental Biology 2024Quote: ... column and the sample chamber was filled with E3 supplemented with 0.2 mM 1-phenyl-2-thiourea (10107703, Acros Organics) and 0.04% tricaine methanesulfonate (MS-222 ...
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bioRxiv - Cell Biology 2023Quote: ... ApoER2 plasmids were co-transfected with RAP plasmid at a 1:2 ratio in Opti-MEM medium (Gibco, Thermo Fisher) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... ApoER2 plasmids were co-transfected with RAP plasmid at a 1:2 ratio in Opti-MEM medium (Gibco, Thermo Fisher) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... After 2 hours incubation with HRP-conjugated secondary antibody (1:10,000; anti-mouse or anti-rabbit, Invitrogen #31430 and #31460) in TBST ...
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bioRxiv - Developmental Biology 2024Quote: ... goat-anti mouse Alexa Fluor 488 or 568) combined with 2 drops of NucBlue in 1 ml PBT (Invitrogen, RF37606), for 1-2 days ...
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bioRxiv - Immunology 2023Quote: ... The appropriate volume of cells was then pelleted at 1500rpm for 5 minutes after which the supernatant was decanted and the cells were resuspended at a 2:1 ratio of RPMI media (Gibco) + FLIPR Calcium 6 dye (Molecular Devices) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were centrifuged at 500 ×g for 5 minutes at 4°C and washed and resuspended with ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS) ...
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bioRxiv - Biochemistry 2024Quote: ... polyethyleneimine (PEI) was used as a transfection reagent and HEK293T cells were incubated with DNA:PEI (1:2) in OPTIMEM (Life Technologies) for two hours ...
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bioRxiv - Cell Biology 2024Quote: Synchronized day 1 adult worm populations were collected in M9 Buffer and then diluted with 2× LDS Sample Buffer (Invitrogen). The samples were subjected to a controlled heat treatment to induce disorder in protein conformations ...
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bioRxiv - Cell Biology 2024Quote: ... both at 1:300 dilution and mounted with ProLong Gold antifade reagent with DAPI (4’,6-diamidino-2-phenylindole; Invitrogen). Confocal microscopy (Olympus FLUOVIEW FV3000 ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids developed within 7 - 21 days after initial seeding and were first passaged (1:2) by dissociation using TrypLE Express Enzyme (ThermoFisher) and resuspension in OcellO primary organoid medium ...
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bioRxiv - Cancer Biology 2023Quote: ... Subsequent dissociation was performed in reduced serum media (DMEM media; #41966 with 2% FBS; #10270106, 1% Pen-strep; #15140122, and 25mM HEPES; #15630056, all products from Gibco) supplemented with Collagenase IV (1mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with 0.1% Triton X-100 in PBS supplemented with 4′,6-diamidino-2-phenylindole (DAPI) (1 μg/ml) (Invitrogen, # 62248) for 30 min ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... the larvae were exposed to 0.25% sodium dodecyl sulfate (SDS) for 2 min to permeabilize the cuticle followed by 1 h incubation with 10 µg/mL Hoechst 33342 (ThermoFisher) or 30 µM DRM oligoTEA linker and 10 µg/mL Hoechst 33342 ...
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bioRxiv - Neuroscience 2023Quote: ... incubation with primary antisera was followed by 2 hours of incubation with secondary antibody (1:350, goat anti-mouse IgG Alexa Fluor 488; Cat#A1101, Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... pMDG2.1-VSV-G and p8.91-GAG-POL in a ratio of 4:1:2 to a total of 10 μg of DNA using Lipofectamine 2000 (ThermoFisher) as per the manufacturer’s instructions overnight ...
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bioRxiv - Neuroscience 2023Quote: ... with TBST and incubated for 2 hours with horseradish peroxidase coupled secondary goat anti-rabbit or anti-mouse IgG (1:5000, Invitrogen) in TBST ...
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bioRxiv - Immunology 2022Quote: ... The dissociated cells were washed 3 times with chilled PBS containing 1% heat-inactivated FBS and 2 mM EDTA (Gibco).
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bioRxiv - Microbiology 2022Quote: ... Resulting cDNA was diluted 1:2 and used for real-time PCR with Power SYBR Green (Thermo Fisher Scientific 4367659) and Applied Biosystems 7500 real time PCR system ...
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bioRxiv - Genetics 2023Quote: ... differentiation was initiated by switching to DMEM-F12/Neurobasal media (2:1) supplemented with N2 and retinol-free B27 (N2B27 RA−; Gibco). For the first ten days ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed and stained with 1:10,000 DAPI (4=,6-diamidino-2-phenylindole, Thermo Fisher Scientific; catalog #: D3571) for 15 minutes at room temperature before coverslips were applied using PBS + glycerol as mounting medium.
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with anti-chicken Alexa Fluor 488 and/or anti-mouse Alexa Fluor 555 secondary antibodies (1:250) for 2 hours before mounting with Prolong Glass (Thermofisher). Confocal z-stacks were obtained by imaging on a Zeiss LSM 700 microscope in the UNC Microscopy Services Laboratory with an EC Plan Neofluar 40x objective using a 1.3 N.A ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were incubated with 1:1000 diluted PE-conjugated F(ab’)2-anti-human-kappa-light-chain (Invitrogen, PA1-74408) in PBS/ 0.1% BSA (20 min ...
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bioRxiv - Immunology 2023Quote: ... resuspended in solubilization buffer (9M Urea containing 1% DTT and 2% Triton X and NuPAGE lithium dodecyl sulfate sample buffer 4X (Invitrogen) at a 3:1 ratio) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed with FACS buffer (HBSS with 2% fetal bovine serum) and resuspended in FACS buffer containing propidium iodide (PI; 1:2000; Invitrogen). Panel 1 samples were measured with a FACS Canto II (BD Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: Full-length cDNA sequence of Hs WDR23 Isoforms 1 and 2 were cloned into pcDNA 6.2/N-EmGFP/TOPO (Thermo Fisher), as previously described [19] ...
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bioRxiv - Neuroscience 2023Quote: ... and the nuclei pellet was resuspended in 2 mL of ice-cold resuspension buffer (1 M HEPES [#J16924- AE, ThermoFisher Scientific] ...
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bioRxiv - Cell Biology 2023Quote: ... retinas were incubated on a rocking platform for 2 hours at room temperature (RT) in Claudio’s blocking buffer (CBB) consisting of 1% FBS (LTID 10500-064, Thermo Fisher), 3% BSA (MB04602 ...
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bioRxiv - Developmental Biology 2023Quote: ... The 2° antibodies used were Invitrogen™ Alexa Fluor™ Plus 405 Phalloidin (1:100, Fisher Scientific, Cat. No. A30104), Alexa Fluor 647® Goat anti-Mouse IgG2b Cross-Adsorbed Secondary Antibody (1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted 1:1000 in 2% goat serum/PBS for 1 hour at room temperature and mounted in ProLong Glass mounting media (ThermoFisher). Each slide was then imaged with a Leica Thunder microscope or Zeiss Axio Imager using the Zeiss AxioVision 4.8 software or Leica DM6 B using LASX software ...
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bioRxiv - Neuroscience 2023Quote: ... was diluted in assay buffer (2 μM in 10 mM HEPES, 150 mM NaCl) containing Sypro Orange (1 in 1000 dilution; Thermofisher). Compounds ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was DNase digested for 30 min at 37°C using 1 µl TURBO DNase (2 U/µl) (Invitrogen). A 500-ng aliquot of the isolated RNA was converted to cDNA with the iScript Advanced Kit (Bio-Rad ...
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bioRxiv - Genomics 2023Quote: ... cDNA was synthesized from 1-2 μg total RNA with the Multiscribe High-Capacity cDNA Reverse Transcription Kit (ThermoFisher 4368814).
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bioRxiv - Genetics 2023Quote: Samples were then incubated at 37 °C for 1 h after addition of 2 μl of RNase Cocktail Enzyme Mix (Ambion). Then 40 μg of Proteinase K (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... The filtrate was then incubated for 1 h rotating at 4 °C with 2 mL Ni-NTA resin (Thermo Fisher) per 1.5 L of expression culture ...
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bioRxiv - Developmental Biology 2023Quote: ... The mRNA from plasmid #1 and #2 was transcribed using the mMESSAGE mMACHINE SP6 Transcription Kit (Thermo Fisher Scientific, AM1340) and purified with the NucleoSpin RNA Clean-up XS kit (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA and RNA products at various points of the iTP-seq protocol were analyzed on 9% acrylamide (19:1) TBE (90 mM Tris, 90 mM boric acid, and 2 mM EDTA) gels and stained with SyBR Gold (Invitrogen). Inverse toeprints were excised from 12% acrylamide TBE gels using a clean scalpel ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 h with secondary antibodies diluted in blocking buffer to 1:600 (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen, Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed with assay buffer (PBS containing 0.2% BSA and 1 mM CaCl2) and resuspended in assay buffer with 2 μM Fluo-4-AM (Life Technologies). Fluo-4-AM was prepared as a 1 mM stock in DMSO ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed once in PBS and incubated in the following secondary antibody and lipid droplet dye for 2 hr at RT: donkey anti-rabbit 555 (1:500; Invitrogen) and LipidSpot 488 (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of total RNA was mixed with 2 µl of diluted ERCC RNA spike-in mix (1:100 dilution in nuclease-free H2O; Invitrogen). Ribosomal (r)RNAs were depleted using the Ribominus Eukaryote v2 kit (Ambion) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the DNA template was digested by adding 1 µl of TURBO™ DNase (2 U/µL) (Thermo Fisher Scientific) and incubating for 30 min at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding heavy and light chains were transfected into Expi293F cells in a 2:1 mass ratio using an ExpiFectamine 293 transfection kit (Gibco). Six days post-transfection ...
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bioRxiv - Plant Biology 2023Quote: ... were co-transformed with the plasma membrane marker PM-yk, endoplasmic reticulum ER-gk (Nelson et al., 2007) or stained with 2 µg mL-1 DAPI (D1306; Invitrogen) to stain nuclei ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated o/n at with rotation at 4°C with 2 µg IP antibody and for 1 h with Dynabeads Protein G (Invitrogen). Alternatively ...
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bioRxiv - Plant Biology 2023Quote: RNA in situ hybridization was conducted using Invitrogen ViewRNA ISH Tissue 1-Plex and 2-Plex Assay kits (ThermoFisher Scientific) using the manufacture’s protocol optimized for Medicago root and nodules sections (Kulikova et al. ...
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bioRxiv - Physiology 2023Quote: ... Tissues were then rinsed 2× in PBS and incubated in Bodipy FL Dye (1:500 dilution from a 1mg/mL stock, ThermoFisher) for 30min in PBS ...